背景:鼻咽癌(Nasopharyngeal carcinoma, NPC)是一种源于鼻咽上皮的恶性肿瘤。Alisol A是一种来源于泽泻根茎的三萜类化合物,具有抑制癌细胞生长和诱导癌细胞凋亡的能力。Alisol A对鼻咽癌的影响尚不明确。方法:Western blot检测蛋白表...背景:鼻咽癌(Nasopharyngeal carcinoma, NPC)是一种源于鼻咽上皮的恶性肿瘤。Alisol A是一种来源于泽泻根茎的三萜类化合物,具有抑制癌细胞生长和诱导癌细胞凋亡的能力。Alisol A对鼻咽癌的影响尚不明确。方法:Western blot检测蛋白表达。采用AutoDock Vina和Discovery Studio软件进行分子对接。结果:Alisol A可抑制鼻咽癌细胞的活力、增殖、迁移和侵袭。分子对接模拟实验证实Alisol A与YAP蛋白结合。此外,在鼻咽癌细胞中,Alisol A促进YAP的磷酸化并抑制YAP的表达。结论:Alisol A通过抑制Hippo信号通路抑制鼻咽癌细胞。Alisol A可能是治疗鼻咽癌的候选药物。Background: Nasopharyngeal carcinoma (NPC) is a malignant tumor originating from the nasopharyngeal epithelium. Alisol A, a triterpenoid compound derived from rhizome of Alismatis alismatis, has been shown to inhibit cancer cell growth and induce apoptosis. The effect of Alisol A on nasopharyngeal carcinoma (NPC) is still unclear. Methods: Western blot was used to detect protein expression. AutoDock Vina and Discovery Studio software were used for molecular docking. Results: Alisol A inhibited the viability, proliferation, migration, and invasion of NPC cells. The molecular docking simulation assay confirmed that Alisol A is bound to YAP protein. In addition, Alisol A promoted the phosphorylation of YAP and suppressed the expression of YAP in NPC cells. Conclusion: Alisol A inhibits the growth of NPC cells by inhibiting Hippo signaling pathway. Alisol A may be a candidate drug for the treatment of NPC.展开更多
文摘背景:鼻咽癌(Nasopharyngeal carcinoma, NPC)是一种源于鼻咽上皮的恶性肿瘤。Alisol A是一种来源于泽泻根茎的三萜类化合物,具有抑制癌细胞生长和诱导癌细胞凋亡的能力。Alisol A对鼻咽癌的影响尚不明确。方法:Western blot检测蛋白表达。采用AutoDock Vina和Discovery Studio软件进行分子对接。结果:Alisol A可抑制鼻咽癌细胞的活力、增殖、迁移和侵袭。分子对接模拟实验证实Alisol A与YAP蛋白结合。此外,在鼻咽癌细胞中,Alisol A促进YAP的磷酸化并抑制YAP的表达。结论:Alisol A通过抑制Hippo信号通路抑制鼻咽癌细胞。Alisol A可能是治疗鼻咽癌的候选药物。Background: Nasopharyngeal carcinoma (NPC) is a malignant tumor originating from the nasopharyngeal epithelium. Alisol A, a triterpenoid compound derived from rhizome of Alismatis alismatis, has been shown to inhibit cancer cell growth and induce apoptosis. The effect of Alisol A on nasopharyngeal carcinoma (NPC) is still unclear. Methods: Western blot was used to detect protein expression. AutoDock Vina and Discovery Studio software were used for molecular docking. Results: Alisol A inhibited the viability, proliferation, migration, and invasion of NPC cells. The molecular docking simulation assay confirmed that Alisol A is bound to YAP protein. In addition, Alisol A promoted the phosphorylation of YAP and suppressed the expression of YAP in NPC cells. Conclusion: Alisol A inhibits the growth of NPC cells by inhibiting Hippo signaling pathway. Alisol A may be a candidate drug for the treatment of NPC.
