Objectives To construct a recombinant plasmid carrying enhanced green fluore- scent protein (EGFP) and human vascular endothelial growth factor (VEGF) 121 gene and detect its expre- ssion in rat mesenchymal stem cells...Objectives To construct a recombinant plasmid carrying enhanced green fluore- scent protein (EGFP) and human vascular endothelial growth factor (VEGF) 121 gene and detect its expre- ssion in rat mesenchymal stem cells (MSCs). Methods Human VEGF121 cDNA was amplified with polymerase chain reaction (PCR) from pCD/hVEGF121 and was inserted into the eukaryotic expression vector pEGFP- C1. After being identified with PCR, double enzyme digestion and DNA sequencing. The recombinant plasmid pEGFP/hVEGF121 was transferred into rat MSCs with lipofectamine. The expression of EGFP/VEGF121 fusion protein were detected with fluorescence microscope and immunocytochemical staining respectively. Results The recombinant plasmid was confirmed with PCR, double enzyme digestion and DNA sequencing. The fluoresce- nce microscope and immunocytochemical staining results showed that the EGFP and VEGF121 protein were expressed in MSCs 48 h after transfection. Conclusions The recombinant plasmid carrying EGFP and human VEGF was successfully constructed and expressed positively in rat MSCs. It offers a promise tool for further research on differentiation of MSCs and VEGF gene therapy for ischemial cardiovascular disease.展开更多
This article examines how the twin transitions of digitalisation and decarbonisation are reshaping global trade by encoding environmental rules into data structures,digital carriers and verifcation algorithms,a proces...This article examines how the twin transitions of digitalisation and decarbonisation are reshaping global trade by encoding environmental rules into data structures,digital carriers and verifcation algorithms,a process conceptualised as Green Digital Expression(GDE).It develops a system-oriented“Digital TradeEnvironmentRegulation”model and the notion of algorithmic isomorphism to explain how registry systems,platform logistics tools and infrastructure projects translate green norms into code and thereby reconfgure market access,compliance costs and bargaining power.Comparing an EU-style regulatory coercive path,a platform-based mimetic path and a China-centred infrastructure path,the paper shows that digitalisation can raise green total factor productivity yet simultaneously intensify energy rebound effects and widen a“digital green divide”for the Global South.It concludes with policy recommendations on interoperable standards,support for digital MRV capacity and green IT requirements to align trade facilitation reforms with global climate governance goals.展开更多
文摘Objectives To construct a recombinant plasmid carrying enhanced green fluore- scent protein (EGFP) and human vascular endothelial growth factor (VEGF) 121 gene and detect its expre- ssion in rat mesenchymal stem cells (MSCs). Methods Human VEGF121 cDNA was amplified with polymerase chain reaction (PCR) from pCD/hVEGF121 and was inserted into the eukaryotic expression vector pEGFP- C1. After being identified with PCR, double enzyme digestion and DNA sequencing. The recombinant plasmid pEGFP/hVEGF121 was transferred into rat MSCs with lipofectamine. The expression of EGFP/VEGF121 fusion protein were detected with fluorescence microscope and immunocytochemical staining respectively. Results The recombinant plasmid was confirmed with PCR, double enzyme digestion and DNA sequencing. The fluoresce- nce microscope and immunocytochemical staining results showed that the EGFP and VEGF121 protein were expressed in MSCs 48 h after transfection. Conclusions The recombinant plasmid carrying EGFP and human VEGF was successfully constructed and expressed positively in rat MSCs. It offers a promise tool for further research on differentiation of MSCs and VEGF gene therapy for ischemial cardiovascular disease.
文摘This article examines how the twin transitions of digitalisation and decarbonisation are reshaping global trade by encoding environmental rules into data structures,digital carriers and verifcation algorithms,a process conceptualised as Green Digital Expression(GDE).It develops a system-oriented“Digital TradeEnvironmentRegulation”model and the notion of algorithmic isomorphism to explain how registry systems,platform logistics tools and infrastructure projects translate green norms into code and thereby reconfgure market access,compliance costs and bargaining power.Comparing an EU-style regulatory coercive path,a platform-based mimetic path and a China-centred infrastructure path,the paper shows that digitalisation can raise green total factor productivity yet simultaneously intensify energy rebound effects and widen a“digital green divide”for the Global South.It concludes with policy recommendations on interoperable standards,support for digital MRV capacity and green IT requirements to align trade facilitation reforms with global climate governance goals.
