摘要
目的:构建人Bcl-2基因的转基因表达载体用于转基因动物模型的建立,以便深入探讨脑缺血再灌注损伤中的基因机制。方法:利用RT-PCR技术,用包含bcl-2cDNA的引物从慢性淋巴细胞白血病患者的血液扩增得到一约为708bp的DNA片段,克隆后测序和同源性分析。结果:经RT-PCR扩增获得的产物大小与预计的目的片段大小一致,目的基因测序与国外报道的参考序列相比,序列同源性为99%。结论:以pEGFP-C1载体作为真核表达载体,形成以绿色荧光蛋白作为报告基因的融合基因,可以用于转基因的研究。
AIM:To construct transgenome expressional vector of human bcl-2 gene applied to construct transgenic animals. METHODS: The cDNA primer which include bcl-2 gene was amplified in the blood of chronic lymphatic leukemia patients with RT-PCR. A DNA fragment about 708 bp was acquired from it, and its sequences and isogenies were measured and analyze d after cloning. RESULTS:The production of gene amplification with RT-PCR was identical to the expectation in size. The sequence isogenies of object gene with that of referen ce gene were 99%.
出处
《中国临床康复》
CAS
CSCD
2003年第26期3592-3593,共2页
Chinese Journal of Clinical Rehabilitation
基金
国家自然科学基金资助项目(30070731)~~
关键词
脑
缺血再灌注损伤
BCL-2基因
真核表达载体
绿色荧光蛋白
Using pEGFP-C1 vector as eukaryotic expressional vector and formin g fused gene with green fluorescent protein can be used in the studying of trans genome.
