The low egg production of goose greatly limits the development of the industry.China possesses the most abundant goose breeds resources.In this study,genome resequencing data of swan goose(Anser cygnoides)and domestic...The low egg production of goose greatly limits the development of the industry.China possesses the most abundant goose breeds resources.In this study,genome resequencing data of swan goose(Anser cygnoides)and domesticated high and low laying goose breeds(Anser cygnoides domestiation)were used to identify key genes related to egg laying ability in geese and verify their functions.Selective sweep analyses revealed 416 genes that were specifically selected during the domestication process from swan geese to high laying geese.Furthermore,SNPs and Indels markers were used in GWAS analyses between high and low laying breed geese.The results showed that RTCB,BPIFC,SYN3,SYNE1,VIP,and ESR1 may be related to the differences in laying ability of geese.Notably,only ESR1 was identified simultaneously by GWAS and selective sweep analysis.The genotype of Indelchr3:54429172,located downstream of ESR1,was confirmed to affect the expression of ESR1 in the ovarian stroma and showed significant correlation with body weight at first egg and laying frequency of geese.CCK-8,EdU,and flow cytometry confirmed that ESR1 can promote the apoptosis of goose pre-hierarchical follicles ganulosa cells(phGCs)and inhibit their proliferation.Combined with transcriptome data,it was found ESR1 involved in the function of goose phGCs may be related to MAPK and TGF-beta signaling pathways.Overall,our study used genomic information from different goose breeds to identify an indel located in the downstream of ESR1 associated with goose laying ability.The main pathways and biological processes of ESR1 involved in the regulation of goose laying ability were identified by cell biology and transcriptomics methods.These results are helpful to further understand the laying ability characteristics of goose and improve the egg production of geese.展开更多
分布式控制是智能配电网实现广域保护、控制应用的重要方式,报文的实时性是影响分布式控制性能的关键因素。文中在分析配电网分布式控制应用对通信的要求基础上,对现有的快速报文传输方式进行了比较,提出了基于用户数据报协议(UDP),用...分布式控制是智能配电网实现广域保护、控制应用的重要方式,报文的实时性是影响分布式控制性能的关键因素。文中在分析配电网分布式控制应用对通信的要求基础上,对现有的快速报文传输方式进行了比较,提出了基于用户数据报协议(UDP),用通用面向对象变电站事件(GOOSE)机制传输实时报文(GOOSE over UDP)的方案,可实现快速报文在广域网传输。通过试验平台对该方案的传输性能进行了测试,测得配电终端之间传输延时最大不超过10 ms,满足配电网分布式控制应用的需求。展开更多
基金supported by the China Agriculture Research System of MOF and MARA(CARS-42-4)School Cooperation Project of Ya’an(21SXHZ0028)the Key Technology Support Program of Sichuan Province,China(2021YFYZ0014),for the financial support。
文摘The low egg production of goose greatly limits the development of the industry.China possesses the most abundant goose breeds resources.In this study,genome resequencing data of swan goose(Anser cygnoides)and domesticated high and low laying goose breeds(Anser cygnoides domestiation)were used to identify key genes related to egg laying ability in geese and verify their functions.Selective sweep analyses revealed 416 genes that were specifically selected during the domestication process from swan geese to high laying geese.Furthermore,SNPs and Indels markers were used in GWAS analyses between high and low laying breed geese.The results showed that RTCB,BPIFC,SYN3,SYNE1,VIP,and ESR1 may be related to the differences in laying ability of geese.Notably,only ESR1 was identified simultaneously by GWAS and selective sweep analysis.The genotype of Indelchr3:54429172,located downstream of ESR1,was confirmed to affect the expression of ESR1 in the ovarian stroma and showed significant correlation with body weight at first egg and laying frequency of geese.CCK-8,EdU,and flow cytometry confirmed that ESR1 can promote the apoptosis of goose pre-hierarchical follicles ganulosa cells(phGCs)and inhibit their proliferation.Combined with transcriptome data,it was found ESR1 involved in the function of goose phGCs may be related to MAPK and TGF-beta signaling pathways.Overall,our study used genomic information from different goose breeds to identify an indel located in the downstream of ESR1 associated with goose laying ability.The main pathways and biological processes of ESR1 involved in the regulation of goose laying ability were identified by cell biology and transcriptomics methods.These results are helpful to further understand the laying ability characteristics of goose and improve the egg production of geese.
文摘分布式控制是智能配电网实现广域保护、控制应用的重要方式,报文的实时性是影响分布式控制性能的关键因素。文中在分析配电网分布式控制应用对通信的要求基础上,对现有的快速报文传输方式进行了比较,提出了基于用户数据报协议(UDP),用通用面向对象变电站事件(GOOSE)机制传输实时报文(GOOSE over UDP)的方案,可实现快速报文在广域网传输。通过试验平台对该方案的传输性能进行了测试,测得配电终端之间传输延时最大不超过10 ms,满足配电网分布式控制应用的需求。
