Objective:To prepare and evaluate a glycerol-preserved antigen from an Iranian strain of Leishmania infantum(L infantum) for use in glycerol-preserved direct agglutination tests (GP-DAT) as an alternative to freeze dr...Objective:To prepare and evaluate a glycerol-preserved antigen from an Iranian strain of Leishmania infantum(L infantum) for use in glycerol-preserved direct agglutination tests (GP-DAT) as an alternative to freeze dried direct agglutination teals(FD-DAT) that use freezedried antigen.Methods:Glycerol-preserved DAT antigen was prepared and stored at different temperatures.We tested antigen stored at 4℃,22-37℃and 50℃over a period of 365 days. Seven hundred twenty-nine serum samples were collected from different geographical zones of Iran from 2007-2009,and 80 of these samples were pooled to produce sera.Each pooled serum contained 10 sera.All positive and negative pooled sera were separately tested for anti-L. infantum antibodies with GP-DAT,FD-DAT and formaldehyde-fixed direct agglutination test (FF-DAT) antigens;tests were performed on both human and dog sera over a period of 12 months. Results:There was strong agreement between the results obtained using GP-DAT and FDDAT antigens stored at 22-37℃for 12 months for both human(100%) and dog(100%) pooled sera.The direct agglutination test results were highly reproducible(weighted kappa:GP=0.833, FD=0.979 and FF=0.917).Conclusions:Because GP-DAT antigen is highly stable over a range of temperatures and is easy to transport in the field,this type of antigen may be particularly useful in areas with endemic visceral leishmaniasis.展开更多
目的探讨早期梅尼埃病的畸变产物耳声发射(DPOAE)特点。方法选取22例梅尼埃病患者和15例正常对照,分别于口服甘油前及30 m in后测试DPOAE。结果患者组DPOAE幅值在783、1 105、1 560 H z出现下降,与对照组有明显差异(P<0.05),2 kH z...目的探讨早期梅尼埃病的畸变产物耳声发射(DPOAE)特点。方法选取22例梅尼埃病患者和15例正常对照,分别于口服甘油前及30 m in后测试DPOAE。结果患者组DPOAE幅值在783、1 105、1 560 H z出现下降,与对照组有明显差异(P<0.05),2 kH z以上幅值较对照组偏低,但无明显差异;口服甘油30 m in后,DPOAE幅值在783、1 105、1 560 H z明显升高,与口服前比较有明显差异(P<0.05),较对照组偏低但差异不显著。结论DPOAE有助于早期梅尼埃病的诊断。展开更多
基金funded by Tehran University of Medical Sciences (Project No:88-01-27-9353)National Institute of Health Research,Islamic Republic of Iran(Project No:241/1441)
文摘Objective:To prepare and evaluate a glycerol-preserved antigen from an Iranian strain of Leishmania infantum(L infantum) for use in glycerol-preserved direct agglutination tests (GP-DAT) as an alternative to freeze dried direct agglutination teals(FD-DAT) that use freezedried antigen.Methods:Glycerol-preserved DAT antigen was prepared and stored at different temperatures.We tested antigen stored at 4℃,22-37℃and 50℃over a period of 365 days. Seven hundred twenty-nine serum samples were collected from different geographical zones of Iran from 2007-2009,and 80 of these samples were pooled to produce sera.Each pooled serum contained 10 sera.All positive and negative pooled sera were separately tested for anti-L. infantum antibodies with GP-DAT,FD-DAT and formaldehyde-fixed direct agglutination test (FF-DAT) antigens;tests were performed on both human and dog sera over a period of 12 months. Results:There was strong agreement between the results obtained using GP-DAT and FDDAT antigens stored at 22-37℃for 12 months for both human(100%) and dog(100%) pooled sera.The direct agglutination test results were highly reproducible(weighted kappa:GP=0.833, FD=0.979 and FF=0.917).Conclusions:Because GP-DAT antigen is highly stable over a range of temperatures and is easy to transport in the field,this type of antigen may be particularly useful in areas with endemic visceral leishmaniasis.
文摘目的探讨早期梅尼埃病的畸变产物耳声发射(DPOAE)特点。方法选取22例梅尼埃病患者和15例正常对照,分别于口服甘油前及30 m in后测试DPOAE。结果患者组DPOAE幅值在783、1 105、1 560 H z出现下降,与对照组有明显差异(P<0.05),2 kH z以上幅值较对照组偏低,但无明显差异;口服甘油30 m in后,DPOAE幅值在783、1 105、1 560 H z明显升高,与口服前比较有明显差异(P<0.05),较对照组偏低但差异不显著。结论DPOAE有助于早期梅尼埃病的诊断。
