A novel sesquiterpenoid was isolated from the whole plant of Glechoma longituba. Its structure was elucidated as 1, 8-epoxy-7（11）-germacren-5-one-12, 8-olide 1 on the basis of spectral evidences.
[Objectives]To explore the action mechanism of Glechomae Herba in the treatment of bladder cancer through network pharmacology.[Methods]TCMSP database retrieval and literature text mining were used to find out the mai...[Objectives]To explore the action mechanism of Glechomae Herba in the treatment of bladder cancer through network pharmacology.[Methods]TCMSP database retrieval and literature text mining were used to find out the main chemical active components and their targets,and the"component-target"network map was constructed by Cytoscape 3.7.0 software.The CTD database was used to select targets related to bladder cancer,and the interaction between the targets of the main active components of Glechomae Herba and the targets related to bladder cancer was taken.A total of 87 targets were obtained,then imported into the STRING database to obtain PPI network,and the topological analysis of the network was conducted to find out the key targets.The key targets were introduced into the DAVID database,and the biological process and KEGG(Kyoto Encyclopedia of Genes and Genomes)pathway of the key targets in the treatment of bladder cancer were obtained.[Results]Active compound components interfere with the occurrence and development of bladder cancer possibly through bladder cancer signaling pathway,proteoglycan in cancer signaling pathway,HIF-1 signaling pathway,tumor necrosis factor signaling pathway,PI3K-Akt signaling pathway,toll-like receptor signaling pathway,node-like receptor signaling pathway,and MAPK signaling pathway,etc.[Conclusions]The pharmacological mechanism of Glechomae Herba in the treatment of bladder cancer may be realized through multiple components,multiple targets and multiple pathways.In conclusion,Glechomae Herba has certain medicinal value.展开更多
Aims Most plants are clonal in nature.Clonal ramets can share water,nutrients and photosynthate,especially when they experience patchy resources.Patch contrast(i.e.a difference in resources among patches)and patch dir...Aims Most plants are clonal in nature.Clonal ramets can share water,nutrients and photosynthate,especially when they experience patchy resources.Patch contrast(i.e.a difference in resources among patches)and patch direction(i.e.source–sink relations)are among the basic attributes of spatial patchiness.Here,I hypothesize that young established ramets in nutrient-rich patches support old ramets in nutrient-poor patches when ramets are subjected to different patch contrasts and patch directions.Methods In a greenhouse experiment,old and young ramets of Glechoma longituba were grown in four combinations consisting of patch contrast and patch direction.Minus patch direction refers to a patch combination in which parent ramets grow in nutrient-rich patches while connected daughter ramets grow in nutrient-poor ones and plus patch direction is the opposite direction.Imeasured photosynthesis and fluorescence traits,harvested all ramets,took morphological measures,weighed their dry mass and determined their nutrient uptake and use.Important Findings For parental ramets of G.longituba,patch contrast and patch direction and their interactions had no significant effects on net photosynthetic rate,maximal fluorescence yield,photochemical quenching(quenching refers to any process which decreases the fluorescence intensity of a given substance),non-photochemical quenching,nutrient uptake,biomass and stolon weight ratio.Patch direction alone significantly affected root weight ratio.Large patch contrast enhanced N use efficiency(NUE)and P use efficiency(PUE);plus patch direction decreased NUE,but increased PUE;the patch contrast by patch direction interaction affected PUE and K use efficiency(KUE).There were significant interactions between patch direction and patch contrast on PUE and KUE.It is concluded that soil nutrient patchiness may influence nutrient use strategies,but not nutrient uptake,photosynthesis and growth of parent ramets of G.longituba connected to daughter ramets,and that patch contrast and patch direction jointly affect PUE and KUE.展开更多
采用乙醇提取,硅胶柱反复层析的方法,从白透骨消[Glechoma biondiana(Diels)C.Y.Wu et C.Chen]全草的乙酸乙酯部分分离了11个化合物,利用现代波谱技术(MS,1H-NMR,13C-NMR,DEPT)以及与文献对照的方法鉴定了这些化合物的结构,分别为2α-...采用乙醇提取,硅胶柱反复层析的方法,从白透骨消[Glechoma biondiana(Diels)C.Y.Wu et C.Chen]全草的乙酸乙酯部分分离了11个化合物,利用现代波谱技术(MS,1H-NMR,13C-NMR,DEPT)以及与文献对照的方法鉴定了这些化合物的结构,分别为2α-羟基乌苏酸(1)、2α,3α-二羟基乌苏-12-烯-28-酸(2)、乌苏酸(3)、2α,3α,23-三羟基乌苏-12-烯-28-酸(4)、3β,24-二羟基乌苏-12-烯-28-酸(5)、3α-羟基乌苏-12-烯-28-酸(6)、豆甾烷-3,6-二酮(7)、7β-羟基谷甾醇(8)、豆甾醇(9)、β-胡萝卜苷(10)、棕榈酸(11)。除化合物(3)和(10)外,其余均为首次从该植物中分离得到。展开更多
基金the National Natural Science Foundation of China [No. 30460150] the Previous Special Research Program of Significant Foundation Research 2004CCA03800.
文摘A novel sesquiterpenoid was isolated from the whole plant of Glechoma longituba. Its structure was elucidated as 1, 8-epoxy-7（11）-germacren-5-one-12, 8-olide 1 on the basis of spectral evidences.
基金Project of National Natural Science Foundation of China(82060303)Natural Science Foundation Project of Guangxi(2018GXNSFBA294007)Open Project of the Ministry of Education's Key。
文摘[Objectives]To explore the action mechanism of Glechomae Herba in the treatment of bladder cancer through network pharmacology.[Methods]TCMSP database retrieval and literature text mining were used to find out the main chemical active components and their targets,and the"component-target"network map was constructed by Cytoscape 3.7.0 software.The CTD database was used to select targets related to bladder cancer,and the interaction between the targets of the main active components of Glechomae Herba and the targets related to bladder cancer was taken.A total of 87 targets were obtained,then imported into the STRING database to obtain PPI network,and the topological analysis of the network was conducted to find out the key targets.The key targets were introduced into the DAVID database,and the biological process and KEGG(Kyoto Encyclopedia of Genes and Genomes)pathway of the key targets in the treatment of bladder cancer were obtained.[Results]Active compound components interfere with the occurrence and development of bladder cancer possibly through bladder cancer signaling pathway,proteoglycan in cancer signaling pathway,HIF-1 signaling pathway,tumor necrosis factor signaling pathway,PI3K-Akt signaling pathway,toll-like receptor signaling pathway,node-like receptor signaling pathway,and MAPK signaling pathway,etc.[Conclusions]The pharmacological mechanism of Glechomae Herba in the treatment of bladder cancer may be realized through multiple components,multiple targets and multiple pathways.In conclusion,Glechomae Herba has certain medicinal value.
基金National Natural Science Foundation of China(40435014).
文摘Aims Most plants are clonal in nature.Clonal ramets can share water,nutrients and photosynthate,especially when they experience patchy resources.Patch contrast(i.e.a difference in resources among patches)and patch direction(i.e.source–sink relations)are among the basic attributes of spatial patchiness.Here,I hypothesize that young established ramets in nutrient-rich patches support old ramets in nutrient-poor patches when ramets are subjected to different patch contrasts and patch directions.Methods In a greenhouse experiment,old and young ramets of Glechoma longituba were grown in four combinations consisting of patch contrast and patch direction.Minus patch direction refers to a patch combination in which parent ramets grow in nutrient-rich patches while connected daughter ramets grow in nutrient-poor ones and plus patch direction is the opposite direction.Imeasured photosynthesis and fluorescence traits,harvested all ramets,took morphological measures,weighed their dry mass and determined their nutrient uptake and use.Important Findings For parental ramets of G.longituba,patch contrast and patch direction and their interactions had no significant effects on net photosynthetic rate,maximal fluorescence yield,photochemical quenching(quenching refers to any process which decreases the fluorescence intensity of a given substance),non-photochemical quenching,nutrient uptake,biomass and stolon weight ratio.Patch direction alone significantly affected root weight ratio.Large patch contrast enhanced N use efficiency(NUE)and P use efficiency(PUE);plus patch direction decreased NUE,but increased PUE;the patch contrast by patch direction interaction affected PUE and K use efficiency(KUE).There were significant interactions between patch direction and patch contrast on PUE and KUE.It is concluded that soil nutrient patchiness may influence nutrient use strategies,but not nutrient uptake,photosynthesis and growth of parent ramets of G.longituba connected to daughter ramets,and that patch contrast and patch direction jointly affect PUE and KUE.
文摘采用乙醇提取,硅胶柱反复层析的方法,从白透骨消[Glechoma biondiana(Diels)C.Y.Wu et C.Chen]全草的乙酸乙酯部分分离了11个化合物,利用现代波谱技术(MS,1H-NMR,13C-NMR,DEPT)以及与文献对照的方法鉴定了这些化合物的结构,分别为2α-羟基乌苏酸(1)、2α,3α-二羟基乌苏-12-烯-28-酸(2)、乌苏酸(3)、2α,3α,23-三羟基乌苏-12-烯-28-酸(4)、3β,24-二羟基乌苏-12-烯-28-酸(5)、3α-羟基乌苏-12-烯-28-酸(6)、豆甾烷-3,6-二酮(7)、7β-羟基谷甾醇(8)、豆甾醇(9)、β-胡萝卜苷(10)、棕榈酸(11)。除化合物(3)和(10)外,其余均为首次从该植物中分离得到。
基金This work was supported by the National Natural ScienceFoundation of China(30460150)the Previous Special Research ProgramofSignificant Fundation Research(2004CCA03800)
