In order to explore whether the protective function of GST-pi can prevent transformation in vitro, NIH3T3 cells and carcinogen glycidyl methacrylate (GMA) have been used in cell transformation study. NIH3T3 cells have...In order to explore whether the protective function of GST-pi can prevent transformation in vitro, NIH3T3 cells and carcinogen glycidyl methacrylate (GMA) have been used in cell transformation study. NIH3T3 cells have been transfected with GST-pi cDNA inserted retrovirus vector, pXT1, and then G418 resistant clones have been analyzed by Southern and Northern analyses. NIH3T3/pXGST clones that stably express GST-pi and control cells,展开更多
AIM： To investigate the possible role of c-erbB-2 and glutathione S-transferase （GST-Pi） in primary hepatocellular carcinogenesis and the relationship between liver hyperplastic nodule （LHN）, liver cirrhosis （LC...AIM： To investigate the possible role of c-erbB-2 and glutathione S-transferase （GST-Pi） in primary hepatocellular carcinogenesis and the relationship between liver hyperplastic nodule （LHN）, liver cirrhosis （LC）, and hepatocellular carcinoma （HCC）.METHODS： The expression of c-erbB-2 and GST-Pi was detected immunohistochemically in 42 tissue specimens of HCC and 77 specimens of its adjacent tissue.RESULTS： The positive expression of c-erbB-2 in LHN （28.6%） was significantly higher than that in LC （0%） （P = 0.032〈0.05）, but no significant difference was seen between HCC and LHN or LC （P〉0.05, X^2 = 0.002, 3.447）.The positive expression of GST-Pi in HCC （89.6%） or LHN （72.2%） was significantly higher than that in LC（22.9%, P〈0.001, ;X^2= 49.91, 16.96）. There was a significant difference between HCC and LHN （P〈0.05,X^2= 6.353）.CONCLUSION： The c-erbB-2 expression is an early event in the pathogenesis of HCC. GST-Pi may be a marker enzyme for immunohistochemical detection of human HCC and its preneoplastic lesions. LHN seems to be a preneoplastic lesion related to hepatocarcinogenesis.展开更多
文摘In order to explore whether the protective function of GST-pi can prevent transformation in vitro, NIH3T3 cells and carcinogen glycidyl methacrylate (GMA) have been used in cell transformation study. NIH3T3 cells have been transfected with GST-pi cDNA inserted retrovirus vector, pXT1, and then G418 resistant clones have been analyzed by Southern and Northern analyses. NIH3T3/pXGST clones that stably express GST-pi and control cells,
基金Supported by the Scientific Research Foundation of Shandong Provincial Education Committee (J94, K26)
文摘AIM： To investigate the possible role of c-erbB-2 and glutathione S-transferase （GST-Pi） in primary hepatocellular carcinogenesis and the relationship between liver hyperplastic nodule （LHN）, liver cirrhosis （LC）, and hepatocellular carcinoma （HCC）.METHODS： The expression of c-erbB-2 and GST-Pi was detected immunohistochemically in 42 tissue specimens of HCC and 77 specimens of its adjacent tissue.RESULTS： The positive expression of c-erbB-2 in LHN （28.6%） was significantly higher than that in LC （0%） （P = 0.032〈0.05）, but no significant difference was seen between HCC and LHN or LC （P〉0.05, X^2 = 0.002, 3.447）.The positive expression of GST-Pi in HCC （89.6%） or LHN （72.2%） was significantly higher than that in LC（22.9%, P〈0.001, ;X^2= 49.91, 16.96）. There was a significant difference between HCC and LHN （P〈0.05,X^2= 6.353）.CONCLUSION： The c-erbB-2 expression is an early event in the pathogenesis of HCC. GST-Pi may be a marker enzyme for immunohistochemical detection of human HCC and its preneoplastic lesions. LHN seems to be a preneoplastic lesion related to hepatocarcinogenesis.
