摘要
目的 检测肺癌耐药蛋白 (Lungresistanceprotein ,LRP)、DNA拓扑异构酶Ⅱ (Topoisomerase ,TOPO Ⅱ )和酸性谷胱甘肽 -S -转移酶 (Glutathion S transferase ,GST Pi)在非小细胞肺癌 (Non smallcelllungcancer ,NSCLC)中的表达。探讨其在NSCLC中的表达水平及其临床相关性。 方法 用SP免疫组化方法分别检测 95例术前未进行化疗的NSCLC组织中LRP、TOPO Ⅱ和GST Pi的表达情况。 结果 在NSCLC中LRP、TOPO Ⅱ和GST Pi的阳性率分别为 64 2 1%( 61 95 ) ;60 % ( 5 7 95 ) ;73 69% ( 70 95 )。TOPO Ⅱ与组织类型相关 ,GST Pi表达与组织类型、细胞分化有关。TOPO Ⅱ与GST Pi之间呈负相关 (P <0 0 5 ) ;LRP、TOPO Ⅱ和GST Pi的阳性表达均与生存率相关 ,且三者协同表达与生存率之间差异有显著性 (P <0 0 1)。 结论 LRP、TOPO Ⅱ和GST Pi在不同类型的NSCLC中均可表达 ,但水平不同。它们与NSCLC的耐药明显相关 ,且有一组有效判断预后的综合指标 ;它们的联合检测 ,将为优化治疗方案 ,提高非小细胞肺癌化疗效果提供科学的依据。
Objective To examine the expression of LRP,TOPO-Ⅱ and GST-Pi in NSCLC and Study on clinical relationship between LRP、TOPO-Ⅱ、GST-Pi Co-expression in NSCLC. Methods S-P immunohistochemicel method was used to examine expression of LRP,TOPO-Ⅱ and GST-Pi in 95 cases of NSCLC,which had not been treated by chemotherapy before surgery. Results Of 95 NSCLC,the positive rates of LRP,TOPO-Ⅱ and GST-Pi were 64.21%(61/95),60%(57/95),73.69%(70/95) respectively.The expression of TOPO-Ⅱ is correlated to histological type.The expression of GST-Pi is correlated to histological type,and the differentiation of tumor cells.negatiue correlation were observed in the co-expression of TOPO-Ⅱ and GST-Pi(P<0.05).The positive rates of LRP,TOPO-Ⅱ and GST-Pi were correlated with survival rate.and significant differences were observed between the three and the survival rate (P<0.01). Conclusion LRP,TOPO-Ⅱ and GST-Pi can express in different types of NSCLC,with different expression levels.and correlate with multidrug resistance of NSCLC There is a group of comprehensive indexes for prognosis.Co-examination of these proteins may provide scientific evidence for optimal therapeutic schedules and for achieving better curative effect in NSCLC.
出处
《中国热带医学》
CAS
2004年第1期17-19,共3页
China Tropical Medicine
