Neural stem cells(NSCs)and glioblastoma stem cells(GSCs)share a complex regulatory landscape in which cholinergic signaling plays a pivotal role in both neural development and tumor progression.While acetylcholine(ACh...Neural stem cells(NSCs)and glioblastoma stem cells(GSCs)share a complex regulatory landscape in which cholinergic signaling plays a pivotal role in both neural development and tumor progression.While acetylcholine(ACh)regulates NSC quiescence and differentiation within neurogenic niches,glioblastoma cells exploit th ese pathways to enhance their adaptability and invasiveness.The involvement of muscarinic(M3)and nicotinic(α7)receptors in both cell types suggests that glioblastoma retains neural progenitor-like traits,contributing to its plasticity and resilience.This article explores the shared cholinergic mechanisms between NSCs and GSCs,highlighting their role in both neural development and glioblastoma progression.展开更多
Background:Microglia and brain macrophages contribute significantly to the tumor microenvironment in highly malignant glioblastoma where they are considered important drivers of tumor progression.A better understandin...Background:Microglia and brain macrophages contribute significantly to the tumor microenvironment in highly malignant glioblastoma where they are considered important drivers of tumor progression.A better understanding of the role of the brain macrophages present in glioblastoma appears crucial for improving therapeutic outcomes,especially in the context of novel immunotherapeutic approaches.Methods:We investigated the regulation of two well-established markers for microglia and brain macrophages,IBA1 and CD163,in relation to glioblastoma tumor necrosis using immunohistochemistry and modality fusion heatmaps of whole slide images obtained from adjacent tissue sections.Results:IBA1 and CD163 showed remarkable differences in relation to glioblastoma tumor necrosis.Generally,IBA1 immunoreactive cells were far less common in necrotic tissue areas than CD163-expressing cells.We also found extensive and frequently diffuse extracellular CD163 deposition,especially in hypocellular necrobiotic tumor regions where IBA1 was typically absent.Conclusions:Resident microglia seem more likely to be important for the diffuse infiltration of glioma cells in hypercellular tissue areas,whereas myeloid macrophages may be the main macrophage population in the wake of tumor necrosis.Since the necrotic niche with its interactions between microglia,brain macrophages,and glioblastoma/glioma stem cells is increasingly recognised as an important factor in tumor progression,further detailed studies of the macrophage populations in glioblastoma are warranted.展开更多
Precast concrete structures have gained popularity due to their advantages.However,the seismic performance of their connection joints remains an area of ongoing research and improvement.Grouted Sleeve Connection(GSC)o...Precast concrete structures have gained popularity due to their advantages.However,the seismic performance of their connection joints remains an area of ongoing research and improvement.Grouted Sleeve Connection(GSC)offers a solution for connecting reinforcements in precast components,but their vulnerability to internal defects,such as construction errors and material variability,can significantly impact performance.This article presents a finite element analysis(FEA)to evaluate the impact of internal grouting defects in GSC on the structural performance of precast reinforced concrete columns.Four finite elementmodels representing GSC with varying degrees of defects were used to investigate the effects on mechanical properties,including bearing capacity,stress-deformation behavior,and stiffness degradation.The study highlights the significant impact of internal grouting defects on the mechanical performance of GSC,with findings indicating a decrease in stiffness,increased plastic deformation,and reduced energy dissipation as the proportion of internal defects rises.The analysis reveals that the internal defects in GSC act as stress concentration points,leading to early crack formation and accelerated damage under cyclic loading.By improving construction quality and reducing the prevalence of grouting defects,the adverse effects on the performance of GSC can be mitigated.Compared to defect-free specimens,those with defects of 30%exhibited a 31.23%reduction in horizontal bearing capacity,highlighting the importance of minimizing defects in practical engineering applications.展开更多
Owing to the constraints of depth sensing technology,images acquired by depth cameras are inevitably mixed with various noises.For depth maps presented in gray values,this research proposes a novel denoising model,ter...Owing to the constraints of depth sensing technology,images acquired by depth cameras are inevitably mixed with various noises.For depth maps presented in gray values,this research proposes a novel denoising model,termed graph-based transform(GBT)and dual graph Laplacian regularization(DGLR)(DGLR-GBT).This model specifically aims to remove Gaussian white noise by capitalizing on the nonlocal self-similarity(NSS)and the piecewise smoothness properties intrinsic to depth maps.Within the group sparse coding(GSC)framework,a combination of GBT and DGLR is implemented.Firstly,within each group,the graph is constructed by using estimates of the true values of the averaged blocks instead of the observations.Secondly,the graph Laplacian regular terms are constructed based on rows and columns of similar block groups,respectively.Lastly,the solution is obtained effectively by combining the alternating direction multiplication method(ADMM)with the weighted thresholding method within the domain of GBT.展开更多
文摘Neural stem cells(NSCs)and glioblastoma stem cells(GSCs)share a complex regulatory landscape in which cholinergic signaling plays a pivotal role in both neural development and tumor progression.While acetylcholine(ACh)regulates NSC quiescence and differentiation within neurogenic niches,glioblastoma cells exploit th ese pathways to enhance their adaptability and invasiveness.The involvement of muscarinic(M3)and nicotinic(α7)receptors in both cell types suggests that glioblastoma retains neural progenitor-like traits,contributing to its plasticity and resilience.This article explores the shared cholinergic mechanisms between NSCs and GSCs,highlighting their role in both neural development and glioblastoma progression.
基金funding but aspects of this work(computer analysis)were supported by an Australian Research Council(ARC)grant,DP150104472(MBG)The Australian Genomics and Outcomes of Glioma project was funded by a Cancer Council NSW Strategic Research Partnership Grant.
文摘Background:Microglia and brain macrophages contribute significantly to the tumor microenvironment in highly malignant glioblastoma where they are considered important drivers of tumor progression.A better understanding of the role of the brain macrophages present in glioblastoma appears crucial for improving therapeutic outcomes,especially in the context of novel immunotherapeutic approaches.Methods:We investigated the regulation of two well-established markers for microglia and brain macrophages,IBA1 and CD163,in relation to glioblastoma tumor necrosis using immunohistochemistry and modality fusion heatmaps of whole slide images obtained from adjacent tissue sections.Results:IBA1 and CD163 showed remarkable differences in relation to glioblastoma tumor necrosis.Generally,IBA1 immunoreactive cells were far less common in necrotic tissue areas than CD163-expressing cells.We also found extensive and frequently diffuse extracellular CD163 deposition,especially in hypocellular necrobiotic tumor regions where IBA1 was typically absent.Conclusions:Resident microglia seem more likely to be important for the diffuse infiltration of glioma cells in hypercellular tissue areas,whereas myeloid macrophages may be the main macrophage population in the wake of tumor necrosis.Since the necrotic niche with its interactions between microglia,brain macrophages,and glioblastoma/glioma stem cells is increasingly recognised as an important factor in tumor progression,further detailed studies of the macrophage populations in glioblastoma are warranted.
文摘Precast concrete structures have gained popularity due to their advantages.However,the seismic performance of their connection joints remains an area of ongoing research and improvement.Grouted Sleeve Connection(GSC)offers a solution for connecting reinforcements in precast components,but their vulnerability to internal defects,such as construction errors and material variability,can significantly impact performance.This article presents a finite element analysis(FEA)to evaluate the impact of internal grouting defects in GSC on the structural performance of precast reinforced concrete columns.Four finite elementmodels representing GSC with varying degrees of defects were used to investigate the effects on mechanical properties,including bearing capacity,stress-deformation behavior,and stiffness degradation.The study highlights the significant impact of internal grouting defects on the mechanical performance of GSC,with findings indicating a decrease in stiffness,increased plastic deformation,and reduced energy dissipation as the proportion of internal defects rises.The analysis reveals that the internal defects in GSC act as stress concentration points,leading to early crack formation and accelerated damage under cyclic loading.By improving construction quality and reducing the prevalence of grouting defects,the adverse effects on the performance of GSC can be mitigated.Compared to defect-free specimens,those with defects of 30%exhibited a 31.23%reduction in horizontal bearing capacity,highlighting the importance of minimizing defects in practical engineering applications.
基金National Natural Science Foundation of China(No.62372100)。
文摘Owing to the constraints of depth sensing technology,images acquired by depth cameras are inevitably mixed with various noises.For depth maps presented in gray values,this research proposes a novel denoising model,termed graph-based transform(GBT)and dual graph Laplacian regularization(DGLR)(DGLR-GBT).This model specifically aims to remove Gaussian white noise by capitalizing on the nonlocal self-similarity(NSS)and the piecewise smoothness properties intrinsic to depth maps.Within the group sparse coding(GSC)framework,a combination of GBT and DGLR is implemented.Firstly,within each group,the graph is constructed by using estimates of the true values of the averaged blocks instead of the observations.Secondly,the graph Laplacian regular terms are constructed based on rows and columns of similar block groups,respectively.Lastly,the solution is obtained effectively by combining the alternating direction multiplication method(ADMM)with the weighted thresholding method within the domain of GBT.
