目的:探讨电针治疗帕金森病(PD)的作用机制。方法:50只雄性SD大鼠随机分为正常组、假手术组,每组10只,模型组、电针组,每组15只。采用6-羟基多巴胺(6-OHDA)单侧纹状体立体定向微量注射法制备PD大鼠旋转模型。假手术组注射方法和注射部...目的:探讨电针治疗帕金森病(PD)的作用机制。方法:50只雄性SD大鼠随机分为正常组、假手术组,每组10只,模型组、电针组,每组15只。采用6-羟基多巴胺(6-OHDA)单侧纹状体立体定向微量注射法制备PD大鼠旋转模型。假手术组注射方法和注射部位同模型组,仅注射含0.2%维生素C的0.9%氯化钠溶液。电针组在模型制作成功后给予电针"风府、太冲"穴治疗,每天一次,每次30min,7d为1个疗程,连续治疗2个疗程。各组大鼠选取10只进行取材及处理。运用HPLC荧光法检测纹状体谷氨酸(Glu)浓度,运用RT-PCR法检测谷氨酸转运体-1(GLT-1)m RNA的表达。结果:模型组大鼠GLT-1 m RNA的表达水平较正常组及假手术组显著降低,Glu浓度显著升高(P<0.01),电针组GLT-1 m RNA较模型组显著升高(P<0.05),Glu浓度降低(P<0.05)。结论:电针对PD多巴胺能神经元的保护和促进修复作用可能与电针提高了脑内谷氨酸转运体GLT-1的表达和活性,减轻了细胞外Glu引起的细胞毒作用有关。展开更多
Objective: To observe the effect of subarachnoid nerve block anesthesia on glutamate transporter glutamate-aspartate transporter(GLAST) and GLT-1 expressions in rabbits, and to investigate the effect of peripheral ner...Objective: To observe the effect of subarachnoid nerve block anesthesia on glutamate transporter glutamate-aspartate transporter(GLAST) and GLT-1 expressions in rabbits, and to investigate the effect of peripheral nerve anesthesia on the morphology and function of the spinal cord. Methods: Twenty healthy New Zealand white rabbits were randomly divided into two groups: the experimental group and control group; with 10 rabbits in each group. For spinal nerve anesthesia, 5 g/L of bupivacaine was used in the experimental group, and sterile saline was used in the control group. After 30 min of cardiac perfusion, GLAST and GLT-1 protein expression in spinal neurons were detected by immunohistochemistry and immunofluorescence staining. Results: GLAST and GLT-1 protein-positive cells increased in neurons in the experimental group, compared with the control group(P<0.05). Conclusions: After subarachnoid nerve block anesthesia, rabbit glutamate transporter GLAST and GLT-1 expression is increased; and spinal cord nerve cell function is inhibited.展开更多
文摘目的:探讨电针治疗帕金森病(PD)的作用机制。方法:50只雄性SD大鼠随机分为正常组、假手术组,每组10只,模型组、电针组,每组15只。采用6-羟基多巴胺(6-OHDA)单侧纹状体立体定向微量注射法制备PD大鼠旋转模型。假手术组注射方法和注射部位同模型组,仅注射含0.2%维生素C的0.9%氯化钠溶液。电针组在模型制作成功后给予电针"风府、太冲"穴治疗,每天一次,每次30min,7d为1个疗程,连续治疗2个疗程。各组大鼠选取10只进行取材及处理。运用HPLC荧光法检测纹状体谷氨酸(Glu)浓度,运用RT-PCR法检测谷氨酸转运体-1(GLT-1)m RNA的表达。结果:模型组大鼠GLT-1 m RNA的表达水平较正常组及假手术组显著降低,Glu浓度显著升高(P<0.01),电针组GLT-1 m RNA较模型组显著升高(P<0.05),Glu浓度降低(P<0.05)。结论:电针对PD多巴胺能神经元的保护和促进修复作用可能与电针提高了脑内谷氨酸转运体GLT-1的表达和活性,减轻了细胞外Glu引起的细胞毒作用有关。
基金supported by Natural Science Foundation of Shandong Province(Y2006C02)
文摘Objective: To observe the effect of subarachnoid nerve block anesthesia on glutamate transporter glutamate-aspartate transporter(GLAST) and GLT-1 expressions in rabbits, and to investigate the effect of peripheral nerve anesthesia on the morphology and function of the spinal cord. Methods: Twenty healthy New Zealand white rabbits were randomly divided into two groups: the experimental group and control group; with 10 rabbits in each group. For spinal nerve anesthesia, 5 g/L of bupivacaine was used in the experimental group, and sterile saline was used in the control group. After 30 min of cardiac perfusion, GLAST and GLT-1 protein expression in spinal neurons were detected by immunohistochemistry and immunofluorescence staining. Results: GLAST and GLT-1 protein-positive cells increased in neurons in the experimental group, compared with the control group(P<0.05). Conclusions: After subarachnoid nerve block anesthesia, rabbit glutamate transporter GLAST and GLT-1 expression is increased; and spinal cord nerve cell function is inhibited.
