摘要
目的:检测急性眼高压后大鼠视网膜GLT-1的表达变化。方法:成年大鼠左眼眼压升高至闪光视网膜电图b波消失的临界眼压且维持缺血60min。实验动物分别存活1、3、7或14d后通过免疫组织化学检测大鼠视网膜GLT-1的表达变化,尼氏染色检测节细胞的变化。结果:急性眼高压后视网膜随着再灌时间的延长,内层视网膜厚度逐渐变薄,节细胞层细胞数目进行性下降。GLT-1阳性产物主要表达于OPL和IPL。急性HIOP后再灌1天时,与正常组相比,此时GLT-1表达增加(P＜0．05)。再灌3天时, GLT-1表达量继续增加。第7天时GLT-1表达开始下调,至14天时,GLT-1表达明显低于3天组,但仍高于正常组(P＜0．05)。结论:急性眼高压可导致视网膜GLT-1表达增加,其机制可能与其自身的保护反应有关。
Objective： To observe the expression of GLT-1 in the rat retina following acute high intraocular pressure （HIOP）. Methods： The intraocular pressure of left eyes in the adult rats were increased to b wave disappearance of flash electroretinogragh and then sustained 60 minutes. After survival for 1,3,7 or14d, the expression of GLT-1 in the rat retina were detected by immunohistochemistry and the cell number in the ganglion cell layer （GCL） were observed by Nissl staining. Results： The cell number in the GCL and the thickness of inner retina were gradually decreased with the increase of survival after HIOP. GLT-1 immunoreacfivity was mainly localized in inner plexiform layer（IPL） and outer plexiform layer（OPL）. Compared to the normal control, the GLT-1 expression significantly increased at day 1 after reperfusion.（P〈0.05）, and furhter increased at 3 days reperfusion. At day 7 and day 14, the GLT-1 expression reduced gradually but still higher than that in normal control group （P〈 0.05）. Conclusion： The results suggest that HIOP can lead to the increase of retinal GLT-1 expression, which is probably related to the retinal protection responses
出处
《现代生物医学进展》
CAS
2007年第6期827-829,共3页
Progress in Modern Biomedicine
基金
国家自然科学基金(30100098
30570979)
