Objective:To investigate die ameliorative effect of dietary inclusion of garlic(Allium sativum)on gentamycin-induced hepatotoxicity in rats.Methods:Adult male rats were randomly divided into four groups with sis anima...Objective:To investigate die ameliorative effect of dietary inclusion of garlic(Allium sativum)on gentamycin-induced hepatotoxicity in rats.Methods:Adult male rats were randomly divided into four groups with sis animals in each group.Groups 1 and 2 were fed basal diet while Groups 3 and 4 were fed diets containing 2%and 4%garlic respeetively for 27 d prior to gentamycin administration.Hepatotoxicity was induced by the intraperitoneal administration at gentamycin(100 mg/kg body weight)for 3 d.The liver and plasma were studied for hepatotoxicity and antioxidant indices.Results:Gentamycin induces hepatic damage as revealed by significant(P<0.05)elevation of liver damage marker enzymes(aspartate transaminase and alanine aminotransferase)and reduction in plasma albumin level.Gentamycin also eaused a significant(P<0.05)alteration in plasma and liver enzymatic(catalase,glutathione and super oxygen dehydrogenises)and non-enzymatic(glutathione and vitamin C)antioxidant indices with concomitant increase in the malondialdehyde content;however,there was a significant(P<0.05)restoration of the antioxidant status coupled with significant(P<0.05)decrease in the tissues malondialdehyde content,following consumption of diets containing garlic.Conclusions:These results suggest that dietary inclusion of garlic powder could protect against gentamycin-induced hepatotoxicity,improve antioxidant status and modulate oxidative stress;a function attributed to their phenolic constituents.展开更多
It was demonstrated pathologically that the histological structures of kidneys were impaired after subcutaneous injection of gentamycin for 16 consecutive days in rats. Laboratory parameters showed that serum blood ur...It was demonstrated pathologically that the histological structures of kidneys were impaired after subcutaneous injection of gentamycin for 16 consecutive days in rats. Laboratory parameters showed that serum blood urea nitrogen(BUN) and creatinine (Cr) elevated, while the indices of the erythrogenic line reduced markedly, and erythropoietin (EPO) was not detectable by ELISA. When the rats received recombinant human erythropoietin (rhEPO) for 5 d, the level of EPO in serum went up, and the indices of reticulocyte, red blood cell (RBC),hemoglobin (Hb) and hematocrit (Hct) improved rapidly, of which the response of reticulocyte was most sensitive. The results suggested that the erythrogenic function of the bone marrow in rats was enhanced after the stimulation of rhEPO, and the effect of rhEPO was dose dependent.展开更多
The expanding resistance of pathogenic bacteria to antibiotics casts a serious threat to the public health.Thus,a new strategy is required to resolve this problem.This study aims to compare the antibacterial impact of...The expanding resistance of pathogenic bacteria to antibiotics casts a serious threat to the public health.Thus,a new strategy is required to resolve this problem.This study aims to compare the antibacterial impact of biosynthesized silver nanoparticles(Bio-AgNPs),gentamycin(GEN),and the conjugation of biosynthesized silver nanoparticles and gentamycin(GEN:Bio-AgNPs)on multidrugresistant Pseudomonas aeruginosa isolates.The characteristic properties of Bio-AgNPs were detected by the following analyses:ultraviolet–visible(UV–Vis)spectroscopy,Fourier transform infrared spectroscopy(FTIR),dynamic light scattering(DLS),Zeta potential,transmission electron microscopy(TEM),and scanning electron microscopy(SEM).Depending on micro-dilution assay,the minimum inhibitory concentration(MIC)of the tested subjects(Bio-AgNPs,GEN,GEN:Bio-AgNPs)are calculated to be 112±400,1536±525,and 49±18.8μg/mL,respectively.The obtained results confirmed that the GEN:Bio-AgNPs have greater potency effect than Bio-AgNPs and GEN alone,showing that AgNPs of low concentration can potentially enhance the effect of GEN against resistant P.aeruginosa.展开更多
Surface functionalization of iron oxide nanoparticles(Fe_(2)O_(3) NPs)with antibiotics is a novel approach that opens the door to drug delivery applications.In the present work,we report iron oxide nanoparticles synth...Surface functionalization of iron oxide nanoparticles(Fe_(2)O_(3) NPs)with antibiotics is a novel approach that opens the door to drug delivery applications.In the present work,we report iron oxide nanoparticles synthesized by chemical co-precipitation method.As-synthesized nanoparticles were characterized using field emission scanning electron microscopy(FESEM),energy dispersive X-ray(EDX),X-ray diffraction(XRD),ultraviolet(UV)-visible(Vis)spectroscopy,Fourier transform infrared(FTIR),and vibrating sample magnetometer(VSM).The poly-shaped Fe_(2)O_(3) NPs of size(34±10)nm with hematite(α-Fe_(2)O_(3))phase were synthesized.The antibacterial activity of chloramphenicol and gentamicin and their formulation with encapsulated iron oxide nanoparticles was investigated by the agar well diffusion technique.Drug-encapsulated Fe_(2)O_(3) NPs showed antibacterial activity against Escherichia coli and Staphylococcus aureus strains,possibly in a dose-dependent manner.Significant effectiveness was confirmed by the increase in the single range of inhibition against the tested microorganisms.Furthermore,the effect of iron oxide nanoparticle concentrations ranging from 1 to 9μg/μL on bacterial growth was examined.展开更多
The aim of this paper was to observe the clinical effect of gentamycin combined with sodium bicarbonate for the prevention of irinotecan-induced diarrhea.A total of 98 patients with stage IV cancers were recruited and...The aim of this paper was to observe the clinical effect of gentamycin combined with sodium bicarbonate for the prevention of irinotecan-induced diarrhea.A total of 98 patients with stage IV cancers were recruited and divided into a prevention group(52 patients)and a control group(46 patients).All patients received the chemotherapy including irinotecan.The prevention group received gentamycin and sodium bicarbonate before the use of irinotecan for 4 days;the control group did not receive any prevention.The use of gentamycin and sodium bicarbonate resulted in signifi-cantly higher stool pH(P<0.001),while the incidence of diarrhea by irinotecan was reduced(prevention group 13.70%versus control group 34.83%;P<0.001).Genta-mycin combined with sodium bicarbonate appears to be useful in preventing the diarrhea induced by irinotecan and reducing the dosage of loperamide andfluid replacement.展开更多
Drug absorptive function of the nasal mucosa has been a new and interesting subject of research in bridging rhinology and pharmacology. The authors applied a solution of gentamycin at a concentration of 40 mg/ml to 15...Drug absorptive function of the nasal mucosa has been a new and interesting subject of research in bridging rhinology and pharmacology. The authors applied a solution of gentamycin at a concentration of 40 mg/ml to 15 dogs at a dosage of 5 mg/kg for each through three routes of administration, ie, the intravenous, intramuscular and iutranasal. The serum levels of gentamyciu from 0 to 180 minutes after receiving the drug were determined with thin-layer scanning chromatographic method. It was found that if the bioavailability of the intravenous route was considered as 100%, the bioavailability of the intranasal route was 80.5%,and that of the intramuscular route was 42％. After giving drops of the drug into nasal cavity in above-mentioned dosage,the serum drug level reached a peak in 10 to 30 min ranging in 3.7 to 6.6 μg/ml. The half-life period of the drug given through this route was about three hours, indicating that the na sal mucosa is apt to be penetrated by gentamycin and allows the drug to be abso展开更多
Background:Urinary tract infections(UTIs),primarily caused by uropathogenic Escherichia coli(UPEC),are a significant global health concern.The complications arise from antibiotic resistance and biofilm formation,which...Background:Urinary tract infections(UTIs),primarily caused by uropathogenic Escherichia coli(UPEC),are a significant global health concern.The complications arise from antibiotic resistance and biofilm formation,which reduce the effectiveness of conventional treatments.This study aims to evaluate the antibiofilm activity of the homeopathic medicine Terebinthinae oleum in potencies 30C,200C,and 1M against UTI-causing E.coli,and to compare its effectiveness with the standard antibiotic Gentamycin.Methods:An in-vitro biofilm model was employed.E.coli biofilms were cultivated in microtiter plates and treated with Terebinthinae oleum(30C,200C,1M)and Gentamycin.Biofilm biomass was assessed through crystal violet staining,and optical density(OD)was measured using an ELISA microplate reader.Results:Among the tested potencies,Terebinthinae oleum 30C showed the most prominent inhibitory activity on E.coli biofilms.The inhibition percentage was compared with Gentamycin as a control.Terebinthinae oleum 30C demonstrated 41.88%inhibition of biofilm biomass,while Gentamycin exhibited up to 78.98%inhibition.Higher potencies of Terebinthinae oleum(200C and 1M)showed reduced activity(30.15%and 24.81%,respectively).Conclusion:Terebinthinae oleum,especially at 30C potency,exhibits measurable antibiofilm activity against E.coli,although less effective than Gentamycin.These findings support its use as a complementary therapy in managing biofilm-associated UTIs,justifying further clinical and immunological research.展开更多
基金Supported by Federal University of Technology,Akure Staff Development Grant No.VCPU/URGC/46
文摘Objective:To investigate die ameliorative effect of dietary inclusion of garlic(Allium sativum)on gentamycin-induced hepatotoxicity in rats.Methods:Adult male rats were randomly divided into four groups with sis animals in each group.Groups 1 and 2 were fed basal diet while Groups 3 and 4 were fed diets containing 2%and 4%garlic respeetively for 27 d prior to gentamycin administration.Hepatotoxicity was induced by the intraperitoneal administration at gentamycin(100 mg/kg body weight)for 3 d.The liver and plasma were studied for hepatotoxicity and antioxidant indices.Results:Gentamycin induces hepatic damage as revealed by significant(P<0.05)elevation of liver damage marker enzymes(aspartate transaminase and alanine aminotransferase)and reduction in plasma albumin level.Gentamycin also eaused a significant(P<0.05)alteration in plasma and liver enzymatic(catalase,glutathione and super oxygen dehydrogenises)and non-enzymatic(glutathione and vitamin C)antioxidant indices with concomitant increase in the malondialdehyde content;however,there was a significant(P<0.05)restoration of the antioxidant status coupled with significant(P<0.05)decrease in the tissues malondialdehyde content,following consumption of diets containing garlic.Conclusions:These results suggest that dietary inclusion of garlic powder could protect against gentamycin-induced hepatotoxicity,improve antioxidant status and modulate oxidative stress;a function attributed to their phenolic constituents.
文摘It was demonstrated pathologically that the histological structures of kidneys were impaired after subcutaneous injection of gentamycin for 16 consecutive days in rats. Laboratory parameters showed that serum blood urea nitrogen(BUN) and creatinine (Cr) elevated, while the indices of the erythrogenic line reduced markedly, and erythropoietin (EPO) was not detectable by ELISA. When the rats received recombinant human erythropoietin (rhEPO) for 5 d, the level of EPO in serum went up, and the indices of reticulocyte, red blood cell (RBC),hemoglobin (Hb) and hematocrit (Hct) improved rapidly, of which the response of reticulocyte was most sensitive. The results suggested that the erythrogenic function of the bone marrow in rats was enhanced after the stimulation of rhEPO, and the effect of rhEPO was dose dependent.
文摘The expanding resistance of pathogenic bacteria to antibiotics casts a serious threat to the public health.Thus,a new strategy is required to resolve this problem.This study aims to compare the antibacterial impact of biosynthesized silver nanoparticles(Bio-AgNPs),gentamycin(GEN),and the conjugation of biosynthesized silver nanoparticles and gentamycin(GEN:Bio-AgNPs)on multidrugresistant Pseudomonas aeruginosa isolates.The characteristic properties of Bio-AgNPs were detected by the following analyses:ultraviolet–visible(UV–Vis)spectroscopy,Fourier transform infrared spectroscopy(FTIR),dynamic light scattering(DLS),Zeta potential,transmission electron microscopy(TEM),and scanning electron microscopy(SEM).Depending on micro-dilution assay,the minimum inhibitory concentration(MIC)of the tested subjects(Bio-AgNPs,GEN,GEN:Bio-AgNPs)are calculated to be 112±400,1536±525,and 49±18.8μg/mL,respectively.The obtained results confirmed that the GEN:Bio-AgNPs have greater potency effect than Bio-AgNPs and GEN alone,showing that AgNPs of low concentration can potentially enhance the effect of GEN against resistant P.aeruginosa.
文摘Surface functionalization of iron oxide nanoparticles(Fe_(2)O_(3) NPs)with antibiotics is a novel approach that opens the door to drug delivery applications.In the present work,we report iron oxide nanoparticles synthesized by chemical co-precipitation method.As-synthesized nanoparticles were characterized using field emission scanning electron microscopy(FESEM),energy dispersive X-ray(EDX),X-ray diffraction(XRD),ultraviolet(UV)-visible(Vis)spectroscopy,Fourier transform infrared(FTIR),and vibrating sample magnetometer(VSM).The poly-shaped Fe_(2)O_(3) NPs of size(34±10)nm with hematite(α-Fe_(2)O_(3))phase were synthesized.The antibacterial activity of chloramphenicol and gentamicin and their formulation with encapsulated iron oxide nanoparticles was investigated by the agar well diffusion technique.Drug-encapsulated Fe_(2)O_(3) NPs showed antibacterial activity against Escherichia coli and Staphylococcus aureus strains,possibly in a dose-dependent manner.Significant effectiveness was confirmed by the increase in the single range of inhibition against the tested microorganisms.Furthermore,the effect of iron oxide nanoparticle concentrations ranging from 1 to 9μg/μL on bacterial growth was examined.
文摘The aim of this paper was to observe the clinical effect of gentamycin combined with sodium bicarbonate for the prevention of irinotecan-induced diarrhea.A total of 98 patients with stage IV cancers were recruited and divided into a prevention group(52 patients)and a control group(46 patients).All patients received the chemotherapy including irinotecan.The prevention group received gentamycin and sodium bicarbonate before the use of irinotecan for 4 days;the control group did not receive any prevention.The use of gentamycin and sodium bicarbonate resulted in signifi-cantly higher stool pH(P<0.001),while the incidence of diarrhea by irinotecan was reduced(prevention group 13.70%versus control group 34.83%;P<0.001).Genta-mycin combined with sodium bicarbonate appears to be useful in preventing the diarrhea induced by irinotecan and reducing the dosage of loperamide andfluid replacement.
文摘Drug absorptive function of the nasal mucosa has been a new and interesting subject of research in bridging rhinology and pharmacology. The authors applied a solution of gentamycin at a concentration of 40 mg/ml to 15 dogs at a dosage of 5 mg/kg for each through three routes of administration, ie, the intravenous, intramuscular and iutranasal. The serum levels of gentamyciu from 0 to 180 minutes after receiving the drug were determined with thin-layer scanning chromatographic method. It was found that if the bioavailability of the intravenous route was considered as 100%, the bioavailability of the intranasal route was 80.5%,and that of the intramuscular route was 42％. After giving drops of the drug into nasal cavity in above-mentioned dosage,the serum drug level reached a peak in 10 to 30 min ranging in 3.7 to 6.6 μg/ml. The half-life period of the drug given through this route was about three hours, indicating that the na sal mucosa is apt to be penetrated by gentamycin and allows the drug to be abso
文摘Background:Urinary tract infections(UTIs),primarily caused by uropathogenic Escherichia coli(UPEC),are a significant global health concern.The complications arise from antibiotic resistance and biofilm formation,which reduce the effectiveness of conventional treatments.This study aims to evaluate the antibiofilm activity of the homeopathic medicine Terebinthinae oleum in potencies 30C,200C,and 1M against UTI-causing E.coli,and to compare its effectiveness with the standard antibiotic Gentamycin.Methods:An in-vitro biofilm model was employed.E.coli biofilms were cultivated in microtiter plates and treated with Terebinthinae oleum(30C,200C,1M)and Gentamycin.Biofilm biomass was assessed through crystal violet staining,and optical density(OD)was measured using an ELISA microplate reader.Results:Among the tested potencies,Terebinthinae oleum 30C showed the most prominent inhibitory activity on E.coli biofilms.The inhibition percentage was compared with Gentamycin as a control.Terebinthinae oleum 30C demonstrated 41.88%inhibition of biofilm biomass,while Gentamycin exhibited up to 78.98%inhibition.Higher potencies of Terebinthinae oleum(200C and 1M)showed reduced activity(30.15%and 24.81%,respectively).Conclusion:Terebinthinae oleum,especially at 30C potency,exhibits measurable antibiofilm activity against E.coli,although less effective than Gentamycin.These findings support its use as a complementary therapy in managing biofilm-associated UTIs,justifying further clinical and immunological research.
