Aim: To compare serum gelsolin levels of women with androgenetic alopecia with PCOS and patients with non-PCOS alopecia. Materials and Methods: A total of 30 PCOS patients who applied to our dermatology clinic with th...Aim: To compare serum gelsolin levels of women with androgenetic alopecia with PCOS and patients with non-PCOS alopecia. Materials and Methods: A total of 30 PCOS patients who applied to our dermatology clinic with the complaint of hair loss and were diagnosed with androgenetic alopecia (AGA) were included in the study. Thirty patients who were not diagnosed with PCOS but were diagnosed with AGA were considered as the control group. Patients in the control group were matched with PCOS in terms of age. AGA was diagnosed in cases of widespread thinning of the hair on the scalp and preservation of the frontal hairline. All participants underwent a complete clinical examination and blood examination. Serum gelsolin levels of both groups were measured by ELISA. Results: The number, age, and BMI values of the participants in both groups were recorded as similar. Serum total testosterone, insulin, HOMA-IR and LH values were significantly higher in the PCOS with AGA compared to the AGA without PCOS. There is no significant difference between groups in terms of serum FSH levels. Serum gelsolin levels of the PCOS group were significantly lower than the control group (160.1 ± 34.2 ng/mL vs. 188.6 ± 46.7 ng/mL, p Conclusions: Serum gelsolin levels in PCOS patients with androgenetic alopecia were found to be significantly lower than in non-PCOS alopecia patient groups.展开更多
Most plant reoviruses encode a type of nonstructural protein that assembles tubular structures to package virions for viral spread in planthopper or leafhopper vectors.These tubules are propelled by actin filaments an...Most plant reoviruses encode a type of nonstructural protein that assembles tubular structures to package virions for viral spread in planthopper or leafhopper vectors.These tubules are propelled by actin filaments and facilitate viruses to overcome transmission barriers in insect vectors.This is known as actin-based tubule motility(ABTM),in which insect proteins,especially actin-associated proteins participate.To better understand the insect components that play a role in the ABTM,the proteins interacting with tubule protein Pns11 of the Rice gall dwarf virus(RGDV)in the leafhopper vector were investigated.We found that gelsolin,an actin-modulating protein,interacted with Pns11 in the yeast-two-hybrid system and Sf9 cells.The interaction and co-localization of gelsolin and Pns11 were also verified in cultured cells and insect bodies of the leafhopper vector.Further,the expression of gelsolin was up-regulated by the RGDV infection both in cultured cells and insects.The knockdown of the gelsolin gene triggered by RNA interference increased viral accumulation,thus increasing the viruliferous rates of the leafhopper vector.This negative association of gelsolin with Pns11 and virus infection revealed that gelsolin negatively affected the ability of the virus to spread by interacting with Pns11 tubules,finally acting to negatively regulate RGDV infection.The results of this study indicate that ABTM is negatively regulated by insects in the coevolution of the insect vector and virus.展开更多
Dear Editor,Myocardial infarction(MI)is a critical condition caused by coronary artery thrombosis,accounting for approximately 15%–20%of deaths annually and ranking among the leading causes of mortality worldwide(Cha...Dear Editor,Myocardial infarction(MI)is a critical condition caused by coronary artery thrombosis,accounting for approximately 15%–20%of deaths annually and ranking among the leading causes of mortality worldwide(Chatterjee and Levy,2020).展开更多
Objective：To study the distribution of gelsolin in human platelet and plasma,and the association with blood-stasis syndrome（BSS） of coronary heart disease（CHD）.Methods：Sixty patients with CHD（30 in BSS group an...Objective：To study the distribution of gelsolin in human platelet and plasma,and the association with blood-stasis syndrome（BSS） of coronary heart disease（CHD）.Methods：Sixty patients with CHD（30 in BSS group and 30 in non-BSS group） and 30 healthy subjects（control group） were included in this study.The classification of the syndrome was based on clinical symptoms and signs.Gelsolin concentration in platelet rich plasma（PRP）,platelet poor plasma（PPP）,filamentous actin（F-actin） and group-specific component globulin （Gc-globulin） of PPP were determined by enzyme-linked immunosorbent assay（ELISA）.The fluorescence intensity of CD62p and cytoplasmic calcium（[Ca^（2＋）]_i） in human platelets of patients and healthy persons was measured with flow cytometry.Results：Compared with the control group,gelsolin in PRP of the BSS group increased significantly（P0.01）,while that in PPP of the BSS and non-BSS groups decreased markedly（P0.05）, the CD62p,[Ca^（2＋）]_i of platelet,F-actin,and Gc-globulin of the BSS and non-BSS groups increased significantly （P0.01）.Compared with the non-BSS group,the gelsolin concentration in PRP of BSS group increased significantly（P0.01）,the[Ca^（2＋）]_i of platelet of the BSS group increased markedly（P0.01）,while the F-actin and Gc-globulin of the BSS group had no statistical defference（P0.05）.Conclusions：Gelsolin concentration in PRP was increased and accompanied by the elevated[Ca^（2＋）]_i of platelet in CHD with BSS,while gelsolin in PPP were lowered markedly.We speculate that plasma gelsolin may clear F-actin from circulation,thus resulting in depletion of plasma gelsolin significantly.This,in addition to the increased calcium influx of platelets,may lead to the gelsolin abnormal expression on platelets during the process of BSS in CHD.Therefore,platelet gelsolin may serve as a new potential biomarker and a therapeutic target of BSS in CHD.展开更多
The villin/gelsolin/fragmin superfamily is a conserved Ca^2＋-dependent family of actin-regulating proteins that is widely present both in mammalian and non-mammalian organisms. They have traditionally been characteri...The villin/gelsolin/fragmin superfamily is a conserved Ca^2＋-dependent family of actin-regulating proteins that is widely present both in mammalian and non-mammalian organisms. They have traditionally been characterized by the same core of three or six tandem gelsolin subdomains. The study in vertebrates and lower eukaryotic cells has revealed that the villin/gelsolin/fragmin superfamily of proteins has versatile functions including severing, capping, nucleating or bundling actin filaments. In plants, encouraging progress has been made in this field of research in recent years. This review will summarize the identified plant homologs of villin/gelsolin/fragmin superfamily, thus providing a basis for reflection on their biochemical activities and functions in plants.展开更多
The effect of plasma gelsolin on plant microfilaments and its localization in plant cells were investigated. The results by using ultracentrifugation and electron microscopy showed that plant microfilaments could be s...The effect of plasma gelsolin on plant microfilaments and its localization in plant cells were investigated. The results by using ultracentrifugation and electron microscopy showed that plant microfilaments could be severed into shorter fragments by gelsolin in a Ca2+-dependent manner. By measuring the binding ability of plasma gelsolin to pollen actin using the method of immunoprecipitation, it was shown that pollen actin could bind gelsolin at a ratio of 2.0±0.21 in the presence of Ca2+. Addition of EGTA could disassociate the actin-gelsolin complexes, reducing the ratio to 1.2±0.23, and the addition of PIP2 could further reduce the ratio to 0.8±0.1. The results indicate that plant actin has similar binding properties with plasma gelsolin as that of animal actin. By Western blotting we identified the existence of gelsolin in lily pollen. The results of immunolo- calization of gelsolin in pollen and pollen tube showed that gelsolin was mainly localized at the germinal furrow in pollen grains and at the cytoplasm in pollen tube, especially in the tip region.展开更多
文摘Aim: To compare serum gelsolin levels of women with androgenetic alopecia with PCOS and patients with non-PCOS alopecia. Materials and Methods: A total of 30 PCOS patients who applied to our dermatology clinic with the complaint of hair loss and were diagnosed with androgenetic alopecia (AGA) were included in the study. Thirty patients who were not diagnosed with PCOS but were diagnosed with AGA were considered as the control group. Patients in the control group were matched with PCOS in terms of age. AGA was diagnosed in cases of widespread thinning of the hair on the scalp and preservation of the frontal hairline. All participants underwent a complete clinical examination and blood examination. Serum gelsolin levels of both groups were measured by ELISA. Results: The number, age, and BMI values of the participants in both groups were recorded as similar. Serum total testosterone, insulin, HOMA-IR and LH values were significantly higher in the PCOS with AGA compared to the AGA without PCOS. There is no significant difference between groups in terms of serum FSH levels. Serum gelsolin levels of the PCOS group were significantly lower than the control group (160.1 ± 34.2 ng/mL vs. 188.6 ± 46.7 ng/mL, p Conclusions: Serum gelsolin levels in PCOS patients with androgenetic alopecia were found to be significantly lower than in non-PCOS alopecia patient groups.
基金supported by the National Natural Science Foundation of China under grant numbers 3173007131772124+2 种基金the Natural Science Foundation of Fujian Province,China under grant number 2017 J06011the National Key R&D Program of China under grant number 2017YFD0200900the Program for New Century Excellent Talents in Fujian Province University under grant number Kla18057A.
文摘Most plant reoviruses encode a type of nonstructural protein that assembles tubular structures to package virions for viral spread in planthopper or leafhopper vectors.These tubules are propelled by actin filaments and facilitate viruses to overcome transmission barriers in insect vectors.This is known as actin-based tubule motility(ABTM),in which insect proteins,especially actin-associated proteins participate.To better understand the insect components that play a role in the ABTM,the proteins interacting with tubule protein Pns11 of the Rice gall dwarf virus(RGDV)in the leafhopper vector were investigated.We found that gelsolin,an actin-modulating protein,interacted with Pns11 in the yeast-two-hybrid system and Sf9 cells.The interaction and co-localization of gelsolin and Pns11 were also verified in cultured cells and insect bodies of the leafhopper vector.Further,the expression of gelsolin was up-regulated by the RGDV infection both in cultured cells and insects.The knockdown of the gelsolin gene triggered by RNA interference increased viral accumulation,thus increasing the viruliferous rates of the leafhopper vector.This negative association of gelsolin with Pns11 and virus infection revealed that gelsolin negatively affected the ability of the virus to spread by interacting with Pns11 tubules,finally acting to negatively regulate RGDV infection.The results of this study indicate that ABTM is negatively regulated by insects in the coevolution of the insect vector and virus.
基金supported by the National Natural Science Foundation of China(82022076)the Young Qihuang Scholar of the“Tens of Millions”Talent project of China(Yue Liu)。
文摘Dear Editor,Myocardial infarction(MI)is a critical condition caused by coronary artery thrombosis,accounting for approximately 15%–20%of deaths annually and ranking among the leading causes of mortality worldwide(Chatterjee and Levy,2020).
基金the National Natural Science Foundation of China (No.81073086)
文摘Objective：To study the distribution of gelsolin in human platelet and plasma,and the association with blood-stasis syndrome（BSS） of coronary heart disease（CHD）.Methods：Sixty patients with CHD（30 in BSS group and 30 in non-BSS group） and 30 healthy subjects（control group） were included in this study.The classification of the syndrome was based on clinical symptoms and signs.Gelsolin concentration in platelet rich plasma（PRP）,platelet poor plasma（PPP）,filamentous actin（F-actin） and group-specific component globulin （Gc-globulin） of PPP were determined by enzyme-linked immunosorbent assay（ELISA）.The fluorescence intensity of CD62p and cytoplasmic calcium（[Ca^（2＋）]_i） in human platelets of patients and healthy persons was measured with flow cytometry.Results：Compared with the control group,gelsolin in PRP of the BSS group increased significantly（P0.01）,while that in PPP of the BSS and non-BSS groups decreased markedly（P0.05）, the CD62p,[Ca^（2＋）]_i of platelet,F-actin,and Gc-globulin of the BSS and non-BSS groups increased significantly （P0.01）.Compared with the non-BSS group,the gelsolin concentration in PRP of BSS group increased significantly（P0.01）,the[Ca^（2＋）]_i of platelet of the BSS group increased markedly（P0.01）,while the F-actin and Gc-globulin of the BSS group had no statistical defference（P0.05）.Conclusions：Gelsolin concentration in PRP was increased and accompanied by the elevated[Ca^（2＋）]_i of platelet in CHD with BSS,while gelsolin in PPP were lowered markedly.We speculate that plasma gelsolin may clear F-actin from circulation,thus resulting in depletion of plasma gelsolin significantly.This,in addition to the increased calcium influx of platelets,may lead to the gelsolin abnormal expression on platelets during the process of BSS in CHD.Therefore,platelet gelsolin may serve as a new potential biomarker and a therapeutic target of BSS in CHD.
基金Supported by the National Natural Science Foundation of China (30630005,30470176 and 30325005)the State Key Basic Research and Development Plan of China (2006CB100100).
文摘The villin/gelsolin/fragmin superfamily is a conserved Ca^2＋-dependent family of actin-regulating proteins that is widely present both in mammalian and non-mammalian organisms. They have traditionally been characterized by the same core of three or six tandem gelsolin subdomains. The study in vertebrates and lower eukaryotic cells has revealed that the villin/gelsolin/fragmin superfamily of proteins has versatile functions including severing, capping, nucleating or bundling actin filaments. In plants, encouraging progress has been made in this field of research in recent years. This review will summarize the identified plant homologs of villin/gelsolin/fragmin superfamily, thus providing a basis for reflection on their biochemical activities and functions in plants.
基金supported by the Major State Basic Research Developmental Program of China(Grant No.G1999011701)the National Natural Science Foundation of China(Grant No.39870052)the Scientific Research Foundation for Outstanding Young Teachers of Ministry of Education of China.
文摘The effect of plasma gelsolin on plant microfilaments and its localization in plant cells were investigated. The results by using ultracentrifugation and electron microscopy showed that plant microfilaments could be severed into shorter fragments by gelsolin in a Ca2+-dependent manner. By measuring the binding ability of plasma gelsolin to pollen actin using the method of immunoprecipitation, it was shown that pollen actin could bind gelsolin at a ratio of 2.0±0.21 in the presence of Ca2+. Addition of EGTA could disassociate the actin-gelsolin complexes, reducing the ratio to 1.2±0.23, and the addition of PIP2 could further reduce the ratio to 0.8±0.1. The results indicate that plant actin has similar binding properties with plasma gelsolin as that of animal actin. By Western blotting we identified the existence of gelsolin in lily pollen. The results of immunolo- calization of gelsolin in pollen and pollen tube showed that gelsolin was mainly localized at the germinal furrow in pollen grains and at the cytoplasm in pollen tube, especially in the tip region.
