Objective To investigate the bone-protective potential of Nelumbo nucifera Gaertn.seed hydroalcoholic extract(NNHE)in an ovariectomized(OVX)rat model by modulating the estrogen receptor/osteoprotegerin/receptor activa...Objective To investigate the bone-protective potential of Nelumbo nucifera Gaertn.seed hydroalcoholic extract(NNHE)in an ovariectomized(OVX)rat model by modulating the estrogen receptor/osteoprotegerin/receptor activator of nuclear factor(NF)-κB(ER/OPG/RANKL)signaling pathway.Methods Network pharmacology was employed with the databases of PubChem,BindingDB,DisGeNET,Gene Ontology(GO),and Kyoto Encyclopedia of Genes and Genomes(KEGG),along with Cytoscape 3.10.2 for identifying the targets and pathways of NNHE relevant to OP.A total of 48 specific pathogen-free(SPF)grade female Wistar rats were randomly divided into six groups(n=8 per group):sham control,OVX control,OVX+NNHE[100,200,400 mg/(kg·d)],and OVX+alendronate[3 mg/(kg·week)].The treatment lasted for 16 weeks.Post-treatment assessment included bone parameters(weight,thickness,density,volume,and length),serum biochemical markers[parathyroid hormone(PTH),estrogen,OPG,RANKL,tartrate-resistant acid phosphatase(TRAP),osteocalcin(OC),calcitonin(CT),calcium(Ca),phosphorus(P),and alkaline phosphatase(ALP)],pro-inflammatory cytokines[tumour necrosis factor(TNF)-α,NF-κB,interleukin(IL)-1β,and IL-6],lipid profiles[total cholesterol(TC),triglycerides(TG),low density lipoprotein(LDL),and high density lipoprotein(HDL)],oxidative stress markers[superoxide dismutase(SOD),catalase(CAT),glutathione peroxidase(GSH),and malondialdehyde(MDA)],and histopathological analyses of femur,uterus,and vaginal tissues.Results Network pharmacology analysis revealed 61 overlapping targets between NNHE and osteoporosis-related genes,including signal transducer and activator of transcription 3(STAT3),NF-κB subunit 1(NFKB1),dopamine receptor D2(DRD2),matrix metalloproteinase 9(MMP9),and caspase-3.GO and KEGG enrichment suggested involvement in the ER/OPG/RANKL signaling pathway.In vivo studies demonstrated that NNHE treatment(400 mg/kg)significantly reduced OVX-induced body weight gain and exhibited estrogenic activity in the vaginal cornification assay.NNHE at 200 and 400 mg/kg significantly increased serum estrogen levels compared with OVX control group,while uterine weight remained unaffected.NNHE significantly improved the lipid profile compared with OVX group,with TC,TG,and LDL decreased,while HDL levels were increased at 200 and 400 mg/kg.Bone metabolism markers were significantly improved compared with OVX group,with serum Ca and P levels restored at all NNHE doses and ALP activity reduced.NNHE effectively modulated bone turnover markers compared with OVX group by reducing levels of OC,TRAP,and PTH,and increasing level of CT.In addition,NNHE decreased RANKL level while increasing OPG level at 200 and 400 mg/kg.Bone mineral density(BMD)was significantly enhanced compared with OVX group.Serum oxidative stress was significantly mitigated compared with OVX group through increased levels of antioxidant enzymes(SOD,CAT,and GSH)and reduced MDA,with the most pronounced effects observed at 400 mg/kg.Pro-inflammatory cytokines(TNF-α,IL-6,IL-1β,and NF-κB)were significantly reduced in all NNHE treatment groups compared with OVX group.Histopathological analysis confirmed restoration of trabecular bone structure and normalization of reproductive tissue morphology in OVX rats after NNHE treatment.Conclusion NNHE demonstrated significant protective effects against OVX-induced osteoporosis through ER/OPG/RANKL signaling pathway modulation,oxidative stress,and inflammation suppression,resulting in improved BMD and structural integrity.These findings indicate that NNHE may represent a promising therapeutic candidate for postmenopausal osteoporosis management and merits further clinical investigation.展开更多
To estimate genetic variation in rhizome lotus （Nelumbo nucifera Gaertn. ssp. nucifera） germplasms in China, a total of 94 rhizome lotus germplasms collected from 18 provinces in China were assessed. The RAPD （rand...To estimate genetic variation in rhizome lotus （Nelumbo nucifera Gaertn. ssp. nucifera） germplasms in China, a total of 94 rhizome lotus germplasms collected from 18 provinces in China were assessed. The RAPD （randomly amplified polymorphic DNA） marker was employed. The selected 17 random primers detected 139 polymorphic alleles out of a total 207 （67.15%）. Nei＇s gene diversity statistics and region differentiation parameters indicated that all germplasms had a relatively high level of genetic diversity with ne = 1.3202, h = 0.1937, I= 0.2982 and the gene flow among all regions was Nrn = 5.5742. The UPGMA dendrogram clustered all 94 germplasms into two clusters： One contained eight commercial cultivars and major landraces, and the other included the wild and some special landraces from five regions, and the PCA analysis exhibited the similar result. Those germplasms from southwestern and eastern China had higher genetic diversity than those from the southern, northern and central China. Predominant proportion of genetic variation （95.61%） was found significant within rather than among （4.39%） regions, as revealed by AMOVA analysis. The data analysis also revealed that the genetic diversity of rhizome lotus germplasms among different regions is positively related to their geographic distances, though it is ambiguous to find the trend from the UPGMA dendrogram and the PCA analysis. A relatively high genetic diversity and gene flow resided in the root lotus germplasms; about 96% of the variation was found within region; accessions from southwest and eastern China have higher genetic diversity than those from the southern, northern and central China.展开更多
Objective:To increase biomass and saponin production in hairy root culture of Talinum paniculatum Gaertn.(T.paniculatum)in balloon-type bubble bioreactor(BTBB).Methods:Hairy roots which were collected from leaf explan...Objective:To increase biomass and saponin production in hairy root culture of Talinum paniculatum Gaertn.(T.paniculatum)in balloon-type bubble bioreactor(BTBB).Methods:Hairy roots which were collected from leaf explants of T.paniculatum were infected by Agrobacterium rhizogenes strain LB510.The hairy roots were cultivated at400 m L Murashige and Skoog liquid medium without growth regulator(MS0)in1 000 m L BTBB.Each BTBB had 2 g hairy roots as initial inoculum and these cultures were treated with various concentrations of sucrose(3%,4%,5%,6%w/v)and potassium nitrate(0.5,1.0,1.5 and 2.0 strength of MS medium).Cultures were maintained for 14days.Fresh and dry weights of hairy roots at the end of culture were investigated.Results:Various concentrations of sucrose influenced the biomass accumulation of hairy roots.Maximum biomass was reached by MS medium supplemented with 6%sucrose and it was approximately threefold higher than control.Culture supplemented with potassium nitrate at 2.0 strength of MS0 could increase biomass accumulation of hairy roots until 0.14 g dry weight and it was almost threefold higher than control.However,the maximum saponin content was obtained by MS medium supplemented with 5%sucrose and 2.0 strength potassium nitrate of MS.Conclusions:Based on this research,those conditions can be used to produce biomass and saponin of hairy root of T.paniculatum in the large scale.展开更多
[ Objectives ] This study was conducted to investigate the genetic diversity of 20 lotus （Nelumbo nucifera Gaertn） samples. [ Methods ] On this optimal ISSR amplification system, 16 primers were screened with good p...[ Objectives ] This study was conducted to investigate the genetic diversity of 20 lotus （Nelumbo nucifera Gaertn） samples. [ Methods ] On this optimal ISSR amplification system, 16 primers were screened with good polymorphism, and the DNA was used to amplify the 20 plant samples. [ R^ults] The 16 primers produced 225 loci, of which 170 were polymorphic, and the polymorphic loci percentage was up to 75.56%. The genetic similarity coefficients between the 20 vari- eties ranged from 0. 577 8 to 0.951 1, which were calculated by POPGENE32. The 20 varieties by UPMGA analysis could be clustered into 2 groups, the first of which included Baiyangdian red lotus and Donggua lotus, and other varieties was included in the second group. [ Conchmions] ISSR molecular markers could be effectively used in genetic diversity and fingerprint analysis for different lotus varieties.展开更多
In order to solve the problem of rapid propagation of Sapindus mukorossi Gaertn seedlings, research on the cutting propagation of S. mukorossi Gaertn was conducted. The results showed that the rooting rate of cuttings...In order to solve the problem of rapid propagation of Sapindus mukorossi Gaertn seedlings, research on the cutting propagation of S. mukorossi Gaertn was conducted. The results showed that the rooting rate of cuttings of the ten clones ranged from 30.9% to 83.0%. Among them, the rooting rate of cuttings of clones SM01 and SM06 was higher than that of other clones, and there were significant differences between different clones in the rooting rate of cuttings. The rooting rate of the semi-lignified shoots having two leaves could be improved significantly. The treatment of the cuttings by GGR6 solutions had significant effects on their rooting rate.展开更多
文摘Objective To investigate the bone-protective potential of Nelumbo nucifera Gaertn.seed hydroalcoholic extract(NNHE)in an ovariectomized(OVX)rat model by modulating the estrogen receptor/osteoprotegerin/receptor activator of nuclear factor(NF)-κB(ER/OPG/RANKL)signaling pathway.Methods Network pharmacology was employed with the databases of PubChem,BindingDB,DisGeNET,Gene Ontology(GO),and Kyoto Encyclopedia of Genes and Genomes(KEGG),along with Cytoscape 3.10.2 for identifying the targets and pathways of NNHE relevant to OP.A total of 48 specific pathogen-free(SPF)grade female Wistar rats were randomly divided into six groups(n=8 per group):sham control,OVX control,OVX+NNHE[100,200,400 mg/(kg·d)],and OVX+alendronate[3 mg/(kg·week)].The treatment lasted for 16 weeks.Post-treatment assessment included bone parameters(weight,thickness,density,volume,and length),serum biochemical markers[parathyroid hormone(PTH),estrogen,OPG,RANKL,tartrate-resistant acid phosphatase(TRAP),osteocalcin(OC),calcitonin(CT),calcium(Ca),phosphorus(P),and alkaline phosphatase(ALP)],pro-inflammatory cytokines[tumour necrosis factor(TNF)-α,NF-κB,interleukin(IL)-1β,and IL-6],lipid profiles[total cholesterol(TC),triglycerides(TG),low density lipoprotein(LDL),and high density lipoprotein(HDL)],oxidative stress markers[superoxide dismutase(SOD),catalase(CAT),glutathione peroxidase(GSH),and malondialdehyde(MDA)],and histopathological analyses of femur,uterus,and vaginal tissues.Results Network pharmacology analysis revealed 61 overlapping targets between NNHE and osteoporosis-related genes,including signal transducer and activator of transcription 3(STAT3),NF-κB subunit 1(NFKB1),dopamine receptor D2(DRD2),matrix metalloproteinase 9(MMP9),and caspase-3.GO and KEGG enrichment suggested involvement in the ER/OPG/RANKL signaling pathway.In vivo studies demonstrated that NNHE treatment(400 mg/kg)significantly reduced OVX-induced body weight gain and exhibited estrogenic activity in the vaginal cornification assay.NNHE at 200 and 400 mg/kg significantly increased serum estrogen levels compared with OVX control group,while uterine weight remained unaffected.NNHE significantly improved the lipid profile compared with OVX group,with TC,TG,and LDL decreased,while HDL levels were increased at 200 and 400 mg/kg.Bone metabolism markers were significantly improved compared with OVX group,with serum Ca and P levels restored at all NNHE doses and ALP activity reduced.NNHE effectively modulated bone turnover markers compared with OVX group by reducing levels of OC,TRAP,and PTH,and increasing level of CT.In addition,NNHE decreased RANKL level while increasing OPG level at 200 and 400 mg/kg.Bone mineral density(BMD)was significantly enhanced compared with OVX group.Serum oxidative stress was significantly mitigated compared with OVX group through increased levels of antioxidant enzymes(SOD,CAT,and GSH)and reduced MDA,with the most pronounced effects observed at 400 mg/kg.Pro-inflammatory cytokines(TNF-α,IL-6,IL-1β,and NF-κB)were significantly reduced in all NNHE treatment groups compared with OVX group.Histopathological analysis confirmed restoration of trabecular bone structure and normalization of reproductive tissue morphology in OVX rats after NNHE treatment.Conclusion NNHE demonstrated significant protective effects against OVX-induced osteoporosis through ER/OPG/RANKL signaling pathway modulation,oxidative stress,and inflammation suppression,resulting in improved BMD and structural integrity.These findings indicate that NNHE may represent a promising therapeutic candidate for postmenopausal osteoporosis management and merits further clinical investigation.
文摘To estimate genetic variation in rhizome lotus （Nelumbo nucifera Gaertn. ssp. nucifera） germplasms in China, a total of 94 rhizome lotus germplasms collected from 18 provinces in China were assessed. The RAPD （randomly amplified polymorphic DNA） marker was employed. The selected 17 random primers detected 139 polymorphic alleles out of a total 207 （67.15%）. Nei＇s gene diversity statistics and region differentiation parameters indicated that all germplasms had a relatively high level of genetic diversity with ne = 1.3202, h = 0.1937, I= 0.2982 and the gene flow among all regions was Nrn = 5.5742. The UPGMA dendrogram clustered all 94 germplasms into two clusters： One contained eight commercial cultivars and major landraces, and the other included the wild and some special landraces from five regions, and the PCA analysis exhibited the similar result. Those germplasms from southwestern and eastern China had higher genetic diversity than those from the southern, northern and central China. Predominant proportion of genetic variation （95.61%） was found significant within rather than among （4.39%） regions, as revealed by AMOVA analysis. The data analysis also revealed that the genetic diversity of rhizome lotus germplasms among different regions is positively related to their geographic distances, though it is ambiguous to find the trend from the UPGMA dendrogram and the PCA analysis. A relatively high genetic diversity and gene flow resided in the root lotus germplasms; about 96% of the variation was found within region; accessions from southwest and eastern China have higher genetic diversity than those from the southern, northern and central China.
基金Supported by Grant from Universitas Airlangga,Surabaya,Indonesia with Grant No.8714/UN3/KR/2013
文摘Objective:To increase biomass and saponin production in hairy root culture of Talinum paniculatum Gaertn.(T.paniculatum)in balloon-type bubble bioreactor(BTBB).Methods:Hairy roots which were collected from leaf explants of T.paniculatum were infected by Agrobacterium rhizogenes strain LB510.The hairy roots were cultivated at400 m L Murashige and Skoog liquid medium without growth regulator(MS0)in1 000 m L BTBB.Each BTBB had 2 g hairy roots as initial inoculum and these cultures were treated with various concentrations of sucrose(3%,4%,5%,6%w/v)and potassium nitrate(0.5,1.0,1.5 and 2.0 strength of MS medium).Cultures were maintained for 14days.Fresh and dry weights of hairy roots at the end of culture were investigated.Results:Various concentrations of sucrose influenced the biomass accumulation of hairy roots.Maximum biomass was reached by MS medium supplemented with 6%sucrose and it was approximately threefold higher than control.Culture supplemented with potassium nitrate at 2.0 strength of MS0 could increase biomass accumulation of hairy roots until 0.14 g dry weight and it was almost threefold higher than control.However,the maximum saponin content was obtained by MS medium supplemented with 5%sucrose and 2.0 strength potassium nitrate of MS.Conclusions:Based on this research,those conditions can be used to produce biomass and saponin of hairy root of T.paniculatum in the large scale.
基金Supported by the Education Department Project of Fujian Province(JB11039)
文摘[ Objectives ] This study was conducted to investigate the genetic diversity of 20 lotus （Nelumbo nucifera Gaertn） samples. [ Methods ] On this optimal ISSR amplification system, 16 primers were screened with good polymorphism, and the DNA was used to amplify the 20 plant samples. [ R^ults] The 16 primers produced 225 loci, of which 170 were polymorphic, and the polymorphic loci percentage was up to 75.56%. The genetic similarity coefficients between the 20 vari- eties ranged from 0. 577 8 to 0.951 1, which were calculated by POPGENE32. The 20 varieties by UPMGA analysis could be clustered into 2 groups, the first of which included Baiyangdian red lotus and Donggua lotus, and other varieties was included in the second group. [ Conchmions] ISSR molecular markers could be effectively used in genetic diversity and fingerprint analysis for different lotus varieties.
基金Supported by Forestry Science and Technology Planning Project of Hunan Province,China(XLK201511)
文摘In order to solve the problem of rapid propagation of Sapindus mukorossi Gaertn seedlings, research on the cutting propagation of S. mukorossi Gaertn was conducted. The results showed that the rooting rate of cuttings of the ten clones ranged from 30.9% to 83.0%. Among them, the rooting rate of cuttings of clones SM01 and SM06 was higher than that of other clones, and there were significant differences between different clones in the rooting rate of cuttings. The rooting rate of the semi-lignified shoots having two leaves could be improved significantly. The treatment of the cuttings by GGR6 solutions had significant effects on their rooting rate.
