摘要
本研究采用多元统计方法(包括方差分析、主成分分析、相关性分析、系统聚类分析及隶属函数评价)对国家作物种质库保存的329份冰草种质资源的5个种子形态学性状指标展开综合分析与评价,旨在构建冰草种质资源评价与创新利用的理论框架。研究结果显示:在供试材料的5个种子表型性状中,千粒重表现出最高的遗传变异,其遗传多样性指数达1.880,变异系数达24.56%;5个性状平均遗传多样性指数为1.752,平均变异系数为14.06%;通过主成分分析提取的3个主要成分可解释98.750%的性状变异,前3个主成分贡献率为65.555%、26.162%和7.034%,完整保留了种子性状的核心遗传信息;相关性分析表明,种子长与其他四个表型性状均呈极显著正相关(P<0.01),种子宽与千粒重、单粒种子面积,千粒重与种子长宽比,种子长宽比与单粒种子面积间亦呈现显著正相关(P<0.01);系统聚类将全部种质划分为4个类群,其中第Ⅰ类群(284份)为中等类群,第Ⅱ类群(41份)为小粒类群,第Ⅲ、第Ⅳ类群各含2份优质特异种质;综合隶属函数评价结果显示,B-288、B-243、B-233、B-250种质在种子长、种子宽、单粒种子面积及千粒重等性状上表现优异,而B-152、B-143、B-128、B-149、B-141、B-153种质的多性状综合表现相对较差。综上,种子长、单粒种子面积和千粒重是决定冰草种质资源种子遗传差异的关键形态学指标。
This study employed multivariate statistical methods,including analysis of variance(ANOVA),principal component analysis(PCA),correlation analysis,hierarchical cluster analysis,and membership function evaluation,to comprehensively analyze and evaluate five seed morphological traits of 329 wildrye(Agropyron cristatum)accessions conserved in the National Crop GeneBank.The objective was to establish a theoretical framework for the evaluation and innovative utilization of A.cristatum(L.)Gaertn germplasm resources.The results showed that among the five phenotypic traits examined,thousand-seed weight exhibited the highest genetic variation,with a genetic diversity index of 1.880 and a coefficient of variation of 24.56%.The average genetic diversity index for all five traits was 1.752,and the average coefficient of variation of 14.06%.PCA extracted three principal components,explaining 98.750%of the trait variation,with the first three main components contributing 65.555%,26.162%,and 7.034%respectively,effectively preserving the core genetic information of seed traits.Correlation analysis demonstrated extremely significant positive correlations between seed length with the other four phenotypic traits(P<0.01).Additionally,significant positive correlations were observed between seed width and both thousand-seed weight and single-seed area(P<0.01),between thousand-seed weight and seed length-width ratio,as well as between seed length-width ratio and single-seed area.Hierarchical cluster analysis categorized the accessions into four groups:GroupⅠ(284 accessions,moderate),GroupⅡ(41 accessions,small-grained),and GroupsⅢandⅣ(two high-quality unique accessions each).Comprehensive evaluation using the membership function identified B-288,B-243,B-233,and B-250 accessions with excellent performance in seed length,seed width,single-seed area,and thousand-seed weight.In contrast,B-152,B-143,B-128,B-149,B-141,and B-153 accessions exhibited relatively weak performance across multiple traits.In conclusion,seed length,single-seed area and thousand-seed weight were identified as the key morphological indicators contributing to the genetic diversity of A.cristatum germplasm.
作者
李悦煊
黄雪琦
白小明
辛霞
LI Yuexuan;HUANG Xueqi;BAI Xiaoming;XIN Xia(Institute of Crop Science,Chinese Academy of Agricultural Sciences,Beijing 100081,China;Pratacultural College,Gansu Agricultural University,Lanzhou 730070,China)
出处
《中国草地学报》
北大核心
2025年第5期34-45,共12页
Chinese Journal of Grassland
基金
中国农业科学院科技创新工程(CAAS-ASTIP-2024-ICS01)。
关键词
冰草
种子表型性状
主成分分析
聚类分析
隶属函数分析
Agropyron cristatum(L.)Gaertn
Seed phenotypic traits
Principal component analysis
Cluster analysis
Membership function analysis
