It has been certified that GABPB1-AS1 is aberrantly expressed and plays as a vital role in some kinds of cancers.However,its expression pattern and functions in non-small cell lung cancer(NSCLC)are still largely unknow...It has been certified that GABPB1-AS1 is aberrantly expressed and plays as a vital role in some kinds of cancers.However,its expression pattern and functions in non-small cell lung cancer(NSCLC)are still largely unknown.This study aims to assess GABPB1-AS1 expression and biological roles in NSCLC.The expression of GABPB1-AS1 was detected in NSCLC specimens and adjacent normal specimens.CCK8 and Transwell assays were performed to evaluate the effects of GABPB1-AS1 on NSCLC cell proliferation,migration and invasion.Bioinformatics tools and luciferase reporter assays were applied to predict and verify GABPB1-AS1’s direct targets.The results revealed that GABPB1-AS1 is sharply reduced in NSCLC specimens and cell lines.CCK8 assays indicated that overexpression of GABPB1-AS1 dramatically reduced NSCLC cell growth,and Transwell assays proved that NSCLC cell migration and invasion were distinctly inhibited by GABPB1-AS1.Exploration of the mechanism uncovered that miRNA-566(miR-566)/F-box protein 47(FBXO47)is directly targeted by GABPB1-AS1 in NSCLC.The study demonstrated that GABPB1-AS1 inhibited NSCLC cell proliferation,migration and invasion by targeting miR-566/FBXO47.展开更多
基金supported by the Highlevel Hospital Construction Research Project of Maoming People’s Hospital[Grant No.zx2020012]Maoming Science and Technology Project Special Fund in 2020[Grant No.2020KJZX014]Maoming Science and Technology Project[Grant No.2020395].
文摘It has been certified that GABPB1-AS1 is aberrantly expressed and plays as a vital role in some kinds of cancers.However,its expression pattern and functions in non-small cell lung cancer(NSCLC)are still largely unknown.This study aims to assess GABPB1-AS1 expression and biological roles in NSCLC.The expression of GABPB1-AS1 was detected in NSCLC specimens and adjacent normal specimens.CCK8 and Transwell assays were performed to evaluate the effects of GABPB1-AS1 on NSCLC cell proliferation,migration and invasion.Bioinformatics tools and luciferase reporter assays were applied to predict and verify GABPB1-AS1’s direct targets.The results revealed that GABPB1-AS1 is sharply reduced in NSCLC specimens and cell lines.CCK8 assays indicated that overexpression of GABPB1-AS1 dramatically reduced NSCLC cell growth,and Transwell assays proved that NSCLC cell migration and invasion were distinctly inhibited by GABPB1-AS1.Exploration of the mechanism uncovered that miRNA-566(miR-566)/F-box protein 47(FBXO47)is directly targeted by GABPB1-AS1 in NSCLC.The study demonstrated that GABPB1-AS1 inhibited NSCLC cell proliferation,migration and invasion by targeting miR-566/FBXO47.
