Background:Fuke Huahuang formulation(FHF)is widely used in the treatment of vaginitis,with clinical evidence indicating its promising anti-inflammatory properties.Methods:We explored the bioactive components and poten...Background:Fuke Huahuang formulation(FHF)is widely used in the treatment of vaginitis,with clinical evidence indicating its promising anti-inflammatory properties.Methods:We explored the bioactive components and potential mechanisms of FHF for treating vaginitis,and reveal its pharmacological activities against vaginitis.Results:A total of 12 anti-inflammatory components in FHF and 584 pharmacological targets were identified.Furthermore,1427 vaginitis-associated targets were identified,and 184 intersection targets between FHF and vaginitis were constructed for network analysis.Gene Ontology and pathway analysis revealed that the therapeutical targets of FHF against vaginitis are involved in modulating inflammatory stress,enhancing immunoregulation,reconstructing the microenvironment,and suppressing cell damage.Molecular docking analysis further suggested the possible direct binding of the bioactive compounds of FHF(fumarine)to the core targets,including AKT Serine/Threonine Kinase 1(AKT1),Signal Transducer and Activator of Transcription 3(STAT3),and nuclear factor-kappaB(NF-κB).Experimental validation found that FHF-treated vaginitis rats exhibited reduced intracellular AKT1,STAT3,and NF-κB protein expressions.Conclusion:Overall,we identified the bioactive compounds and pharmacological mechanisms of FHF against vaginitis,thus offering a theoretical fundament for exploring FHF for treating vaginitis in the future.展开更多
Objective:To systematically evaluate the efficacy and safety of Fuke Qianjin Tablets(妇科千金片)in the treatment of chronic pelvic inflammation.Methods:A systematically and comprehensively search was conducted in 4 Ch...Objective:To systematically evaluate the efficacy and safety of Fuke Qianjin Tablets(妇科千金片)in the treatment of chronic pelvic inflammation.Methods:A systematically and comprehensively search was conducted in 4 Chinese databases of CNKI,VIP,Wan Fang and CBM and the foreign language databases of Pubmed,EMbase and The Cochrane Library.The retrieval time was from database establishment to March 2019.The randomized controlled trials of Fuke Qianjin Tablets(妇科千金片)in the treatment of chronic pelvic inflammation were selected according to the predetermined criteria.The quality of the included study was evaluated by Cochrane collaborative network bias risk evaluation tool,and the meta-analysis was performed by Rev Man5.3 software.Results:A total of 1009 related literatures were searched.After initial screening and strict evaluation,55 studies were included,with a total sample size of 6826 cases,including 3416 cases in the experiment group and 3410 cases in the control group.The results of meta-analysis showed that the total effective rate of Fuke Qianjin Tablets(妇科千金片)combined with antibiotics in the treatment of chronic pelvic inflammation was better than that of antibiotics alone(RR=1.20,95%CI[1.17,1.22],P<0.00001).Fuke Qianjin Tablets(妇科千金片)combined with antibiotics was better than that of antibiotics alone in the improvement of abdominal pain symptoms(RR=1.40,95%CI[1.04,1.88],P<0.00001),leukorrhea abnormality(RR=1.38,95%CI[1.16,1.65],P<0.0004).In terms of safety,Fuke Qianjin Tablets(妇科千金片)combined with antibiotics could reduce the incidence of adverse reactions(RR=0.67,95%CI[0.48,0.93],P<0.02).The main adverse reactions were nausea and vomiting,bitterness and astringency in the mouth,rash and so on.All of them could be tolerated and the symptoms could disappear in the short term,and had no effect on the treatment.Conclusion:Fuke Qianjin Tablets(妇科千金片)combined with antibiotics in the treatment of chronic pelvic inflammation can improve the total effective rate,relieve abdominal pain and abnormal leukorrhea and other clinical discomfort symptoms,improve the quality of life of patients to a certain extent,and no serious adverse reactions are found.Due to the limitation of the quality and quantity of the included literature,the above conclusions need to be further studied and verified by high-quality research.展开更多
Objective:To explore effect of bock greenbrier rhizome capsule combined with Fuke Qianjin Tablet on serum inflammatory factors, immune function and hemorheological index in patients with chronic pelvic inflammation.Me...Objective:To explore effect of bock greenbrier rhizome capsule combined with Fuke Qianjin Tablet on serum inflammatory factors, immune function and hemorheological index in patients with chronic pelvic inflammation.Method:A toal of210 cases of patients with chronic pelvic inflammation were divided into control group (n=104) and observation group (n=106) according to random data table method, control group was given Fuke Qianjin Tablet, observation group was given bock greenbrier rhizome capsule on base of control group, treatment period of both groups was 6 weeks. Compared serum inflammatory factors, immune function and hemorheological index in both groups before and after 6 weeks treatment. Results:Before treatment, comparison of inflammatory factors (TNF-α, IL-6 and IL-8), immune function (CD3+, CD4+, CD8+ and CD4+/CD8+) and hemorheological index (low-cut/high-cut whole blood viscosity, plasma viscosity, FIB) in both groups, there was no statistical significant difference;after treatment, TNF-α, IL-6, IL-8, CD8+, low-cut/ high-cut whole blood viscosity, plasma viscosity, FIB level were lower dramatically than pre-treatment intra-group, moreover, these level in observation group after treatment was lower than control group after treatment;CD3+, CD4+ and CD4+/CD8+ level in both groups after treatment was higher than before treatment intra-group, and these levels in observation group after treatment was higher obviously than control group after treatment.Conclusion:Bock greenbrier rhizome capsule combined with Fuke Qianjin Tablet for patients with chronic pelvic inflammation, its regulated effect on serum inflammatory factors, immune function and hemorheological index in patients with chronic pelvic inflammation was superior to only using Fuke Qianjin Tablet, it was worthy of clinical application.展开更多
文摘Background:Fuke Huahuang formulation(FHF)is widely used in the treatment of vaginitis,with clinical evidence indicating its promising anti-inflammatory properties.Methods:We explored the bioactive components and potential mechanisms of FHF for treating vaginitis,and reveal its pharmacological activities against vaginitis.Results:A total of 12 anti-inflammatory components in FHF and 584 pharmacological targets were identified.Furthermore,1427 vaginitis-associated targets were identified,and 184 intersection targets between FHF and vaginitis were constructed for network analysis.Gene Ontology and pathway analysis revealed that the therapeutical targets of FHF against vaginitis are involved in modulating inflammatory stress,enhancing immunoregulation,reconstructing the microenvironment,and suppressing cell damage.Molecular docking analysis further suggested the possible direct binding of the bioactive compounds of FHF(fumarine)to the core targets,including AKT Serine/Threonine Kinase 1(AKT1),Signal Transducer and Activator of Transcription 3(STAT3),and nuclear factor-kappaB(NF-κB).Experimental validation found that FHF-treated vaginitis rats exhibited reduced intracellular AKT1,STAT3,and NF-κB protein expressions.Conclusion:Overall,we identified the bioactive compounds and pharmacological mechanisms of FHF against vaginitis,thus offering a theoretical fundament for exploring FHF for treating vaginitis in the future.
基金National Key Research and Development Plan Project(2018YFC1707400)Basic Business Expense Project of Central Public Welfare Research Institutes(Z0605)。
文摘Objective:To systematically evaluate the efficacy and safety of Fuke Qianjin Tablets(妇科千金片)in the treatment of chronic pelvic inflammation.Methods:A systematically and comprehensively search was conducted in 4 Chinese databases of CNKI,VIP,Wan Fang and CBM and the foreign language databases of Pubmed,EMbase and The Cochrane Library.The retrieval time was from database establishment to March 2019.The randomized controlled trials of Fuke Qianjin Tablets(妇科千金片)in the treatment of chronic pelvic inflammation were selected according to the predetermined criteria.The quality of the included study was evaluated by Cochrane collaborative network bias risk evaluation tool,and the meta-analysis was performed by Rev Man5.3 software.Results:A total of 1009 related literatures were searched.After initial screening and strict evaluation,55 studies were included,with a total sample size of 6826 cases,including 3416 cases in the experiment group and 3410 cases in the control group.The results of meta-analysis showed that the total effective rate of Fuke Qianjin Tablets(妇科千金片)combined with antibiotics in the treatment of chronic pelvic inflammation was better than that of antibiotics alone(RR=1.20,95%CI[1.17,1.22],P<0.00001).Fuke Qianjin Tablets(妇科千金片)combined with antibiotics was better than that of antibiotics alone in the improvement of abdominal pain symptoms(RR=1.40,95%CI[1.04,1.88],P<0.00001),leukorrhea abnormality(RR=1.38,95%CI[1.16,1.65],P<0.0004).In terms of safety,Fuke Qianjin Tablets(妇科千金片)combined with antibiotics could reduce the incidence of adverse reactions(RR=0.67,95%CI[0.48,0.93],P<0.02).The main adverse reactions were nausea and vomiting,bitterness and astringency in the mouth,rash and so on.All of them could be tolerated and the symptoms could disappear in the short term,and had no effect on the treatment.Conclusion:Fuke Qianjin Tablets(妇科千金片)combined with antibiotics in the treatment of chronic pelvic inflammation can improve the total effective rate,relieve abdominal pain and abnormal leukorrhea and other clinical discomfort symptoms,improve the quality of life of patients to a certain extent,and no serious adverse reactions are found.Due to the limitation of the quality and quantity of the included literature,the above conclusions need to be further studied and verified by high-quality research.
文摘Objective:To explore effect of bock greenbrier rhizome capsule combined with Fuke Qianjin Tablet on serum inflammatory factors, immune function and hemorheological index in patients with chronic pelvic inflammation.Method:A toal of210 cases of patients with chronic pelvic inflammation were divided into control group (n=104) and observation group (n=106) according to random data table method, control group was given Fuke Qianjin Tablet, observation group was given bock greenbrier rhizome capsule on base of control group, treatment period of both groups was 6 weeks. Compared serum inflammatory factors, immune function and hemorheological index in both groups before and after 6 weeks treatment. Results:Before treatment, comparison of inflammatory factors (TNF-α, IL-6 and IL-8), immune function (CD3+, CD4+, CD8+ and CD4+/CD8+) and hemorheological index (low-cut/high-cut whole blood viscosity, plasma viscosity, FIB) in both groups, there was no statistical significant difference;after treatment, TNF-α, IL-6, IL-8, CD8+, low-cut/ high-cut whole blood viscosity, plasma viscosity, FIB level were lower dramatically than pre-treatment intra-group, moreover, these level in observation group after treatment was lower than control group after treatment;CD3+, CD4+ and CD4+/CD8+ level in both groups after treatment was higher than before treatment intra-group, and these levels in observation group after treatment was higher obviously than control group after treatment.Conclusion:Bock greenbrier rhizome capsule combined with Fuke Qianjin Tablet for patients with chronic pelvic inflammation, its regulated effect on serum inflammatory factors, immune function and hemorheological index in patients with chronic pelvic inflammation was superior to only using Fuke Qianjin Tablet, it was worthy of clinical application.
