摘要
基于网络药理学和动物实验探讨妇科千金胶囊治疗盆腔炎性疾病(PID)的作用机制。通过网络药理学获得妇科千金胶囊调控PID的潜在靶点及信号通路。通过动物实验构建PID小鼠模型,给予不同剂量的妇科千金胶囊治疗后观察小鼠子宫外观形态变化;采用苏木精-伊红(HE)染色检测小鼠子宫组织病理变化;酶联免疫吸附测定(ELISA)检测小鼠外周血白细胞介素(IL)-6和肿瘤坏死因子(TNF)-α水平;蛋白免疫印迹法(Western blot)检测小鼠Toll样受体4(TLR4)/磷脂酰肌醇3-激酶(PI3K)/蛋白激酶B(Akt)/核因子-κB(NF-κB)通路相关蛋白表达。网络药理学显示,共得到妇科千金胶囊治疗PID的化合物78个,作用靶点152个,PID疾病靶点1632个,“药物-疾病”交集靶点72个,主要活性成分为β-谷甾醇、豆甾醇、木犀草素、儿茶素等;关键靶点为TNF、IL-6、肿瘤蛋白53(TP53)、IL-1β、胱天蛋白酶3(CASP3)等;主要涉及PI3K/Akt信号通路、IL-17信号通路、脂质代谢与动脉粥样硬化信号通路等。动物实验显示,与模型组相比,妇科千金胶囊能减轻PID小鼠子宫炎性病变,降低子宫组织病理损伤程度,降低血清IL-6、TNF-α水平,下调TLR4、PI3K、Akt、NF-κB p65蛋白的表达。结果表明,妇科千金胶囊能通过多成分、多靶点、多途径发挥治疗PID的作用。同时,其可能通过抑制TLR4/PI3K/Akt/NF-κB通路,减轻炎症反应,改善小鼠子宫组织损伤状态。
This study aims to investigate the mechanism of Fuke Qianjin Capsules in treating pelvic inflammatory disease(PID)based on network pharmacology and animal experiments.The potential targets and signaling pathways of Fuke Qianjin Capsules in regulating PID were predicted by network pharmacology.The mouse model of PID was constructed and administered with different doses of Fuke Qianjin Capsules.After treatment,the uterus tissue of mice was observed for the pathological changes by HE staining.The level of IL-6 and TNF-αin the peripheral blood was measured by ELISA.The protein expression in the TLR4/PI3K/Akt/NF-κB signaling pathways was determined by Western blot.Network pharmacology indicated that a total of 78 compounds,152 targets,1632 PID targets,and 72 intersection targets of"drug-disease"were obtained from Fuke Qianjin Capsules.The main active ingredients includedβ-sitosterol,stigmasterol,luteolin,and catechin.The key targets included TNF,IL-6,TP53,IL-1β,and CASP3.PI3K/Akt,IL-17,and lipid metabolism/atherosclerosis signaling pathways were mainly involved in the treatment.Animal experiments showed that compared with the model group,Fuke Qianjin Capsules alleviated the uterine inflammatory lesions,reduced pathological damage in the uterus tissue,and decreased the serum level of IL-6 and TNF-α,down-regulated the protein level of TLR4,PI3K,Akt,and NF-κB p65 in PID mice.These findings suggest that Fuke Qianjin Capsules can exert therapeutic effects on PID via multiple components,targets,and pathways.Meanwhile,Fuke Qianjin Capsules may reduce the inflammation and attenuate the uterine injuries of PID mice by inhibiting TLR4/PI3K/Akt/NF-κB signaling pathways.
作者
李芊
黄天娇
高聪
王玲
李佳妮
马堃
LI Qian;HUANG Tian-jiao;GAO Cong;WANG Ling;LI Jia-ni;MA Kun(Xiyuan Hospital,China Academy of Chinese Medical Sciences,Beijing 100091,China;First School of Clinical Medicine,Heilongjiang University of Chinese Medicine,Harbin 150040,China;Beijing University of Chinese Medicine,Beijing 100029,China)
出处
《中国中药杂志》
北大核心
2025年第22期6289-6298,共10页
China Journal of Chinese Materia Medica
基金
中国中医科学院第十一批自主选题项目(Z0546)。
关键词
妇科千金胶囊
盆腔炎性疾病
网络药理学
炎性因子
机制
Fuke Qianjin Capsules
pelvic inflammatory disease
network pharmacology
inflammatory cytokine
mechanism
