[Objectives]To analyze the chemical components of Meconopsis integrifolia(Maxim.)Franch total flavonoids(MITF).[Methods]The chemical components of MITF were identified by UPLC-Q-Exactive Orbitrap MS.The column was per...[Objectives]To analyze the chemical components of Meconopsis integrifolia(Maxim.)Franch total flavonoids(MITF).[Methods]The chemical components of MITF were identified by UPLC-Q-Exactive Orbitrap MS.The column was performed using ACQUITY UPLC HSS T3(100 mm×2.1 mm,1.8μm)with 0.1%formic acid water(A)-acetonitrile(B)with gradient elution at 30℃,injection volume of 2μL,and flow rate of 0.3 mL/min.Electrospray ion source adopted positive and negative ion detection mode with scanning range m/z 100-1000.[Results]A total of 93 compounds were identified from MITF,including 57 flavonoids,25 phenolic acids,9 alkaloids and 2 others.Among them,dimethoxytaxifolin was identified as a possible new compound by SciFinder search,and 67 compounds were first identified in M.integrifolia.[Conclusions]This study provides a scientific foundation for clarifying the material basis of the efficacy of M.integrifolia and improving the quality standards.展开更多
[Objective] This study aimed at comparing the four extraction methods of genomic DNA from Clematis fasciculiflora Franch and determining the optimal extraction method for extracting the genomic DNA from Clematis fasci...[Objective] This study aimed at comparing the four extraction methods of genomic DNA from Clematis fasciculiflora Franch and determining the optimal extraction method for extracting the genomic DNA from Clematis fasciculiflora Franch.[Method] Leavies of Clematis fasciculiflora Franch were used as materials for comparing the purity and concentration of extracted DNA and extracting time among the four extraction methods of genomic DNA including improved CTAB method Ⅰ,improved CTAB method Ⅱ,improved CTAB method Ⅲ and improved SDS method.[Result] The four extraction methods could all be successfully used for extracting the genomic DNA from Clematis fasciculiflora Franch.The purity of genomic DNA was the highest using improved CTAB method Ⅰ,with the longest extracting time;while the concentration of genomic DNA was the maximum using the improved SDS method,with the shortest extracting time and relatively low purity;the extracting time of improved CTAB method Ⅲ was the shortest.[Conclusion] This study had established the optimal extraction method for extracting the genomic DNA from Clematis fasciculiflora Franch and supported for the further research using molecular biological methods.展开更多
目的通过研究杀青方式和时间、干燥方式对药用植物独蒜兰Pleione bulbocodioides假鳞茎外观、显微结构和化学成分含量的影响,探索其最佳初加工工艺。方法采用烘箱直接烘干(oven direct drying,OD)、煮制10、20、30 min后烘干(boiling fo...目的通过研究杀青方式和时间、干燥方式对药用植物独蒜兰Pleione bulbocodioides假鳞茎外观、显微结构和化学成分含量的影响,探索其最佳初加工工艺。方法采用烘箱直接烘干(oven direct drying,OD)、煮制10、20、30 min后烘干(boiling for 10,20,30 min and then drying,B10D、B20D、B30D)、隔水蒸制10、20、30 min后烘干(steaming for 10,20,30 min and then drying,S10D、S20D、S30D)和冷冻干燥(freeze drying,FD)共8种方式处理独蒜兰假鳞茎,测定其2-O-葡萄糖基白及苷(dactylorhin A)、白及苷和山药素Ⅲ的含量。通过构建HPLC指纹图谱,并进行相似度分析(similarity analysis,SA)、层次聚类分析(hierarchical cluster analysis,HCA)和主成分分析(principal component analysis,PCA)评价加工方法对假鳞茎质量的影响。通过外观特征、组织结构及粉末显微结构观察,评估加工方法对假鳞茎结构的影响。以dactylorhin A、白及苷、山药素Ⅲ、醇溶性浸出物、水溶性浸出物、折干率、水分、灰分和酸不溶性灰分含量为指标,利用熵权法、层次分析法(analytic hierarchy process,AHP)和逼近理想解排序法(technique for order preference by similarity to ideal solution,TOPSIS)综合评价不同加工方法对假鳞茎品质的影响。结果8种加工方法所得样品的HPLC指纹图谱相似度为0.681~0.994。其中,FD样品的相似度最低,且HCA和PCA均将其单独归为一类,其余7种加工方法的样品则聚为另一类。通过HPLC指纹图谱24个共有峰鉴定出3个共有峰,分别为dactylorhin A(峰7)、白及苷(峰9)和山药素Ⅲ(峰15)。TOPSIS综合评价结果发现,S10D处理样品的贴近度以及dactylorhin A、白及苷和山药素Ⅲ总含量最高。结论隔水蒸制10 min后烘干是独蒜兰假鳞茎初加工的最优方法,该方法有利于活性成分保留,且工艺简便。为独蒜兰假鳞茎的规范化加工以及山慈菇药材质量标准的完善提供了重要依据。展开更多
基金Supported by Natural Science Foundation of Sichuan Province(2023NS-FSC0610)the Special Fund of Natural Science for the Central Universities of Southwest Minzu University(ZYN2023071).
文摘[Objectives]To analyze the chemical components of Meconopsis integrifolia(Maxim.)Franch total flavonoids(MITF).[Methods]The chemical components of MITF were identified by UPLC-Q-Exactive Orbitrap MS.The column was performed using ACQUITY UPLC HSS T3(100 mm×2.1 mm,1.8μm)with 0.1%formic acid water(A)-acetonitrile(B)with gradient elution at 30℃,injection volume of 2μL,and flow rate of 0.3 mL/min.Electrospray ion source adopted positive and negative ion detection mode with scanning range m/z 100-1000.[Results]A total of 93 compounds were identified from MITF,including 57 flavonoids,25 phenolic acids,9 alkaloids and 2 others.Among them,dimethoxytaxifolin was identified as a possible new compound by SciFinder search,and 67 compounds were first identified in M.integrifolia.[Conclusions]This study provides a scientific foundation for clarifying the material basis of the efficacy of M.integrifolia and improving the quality standards.
基金Supported by Applied Basic Research Project of Yunnan Province(2010ZC089)the948Project of National Forestry Bureau(2008-4-11)+1 种基金Sharing Platform Project of Provincial and Ministerial Key Subject,Key Laboratory and School Laboratory of Provincial Colleges and Universities in Yunnan ProvinceScience and Technology Innovation Fund of Southwest Forestry University~~
文摘[Objective] This study aimed at comparing the four extraction methods of genomic DNA from Clematis fasciculiflora Franch and determining the optimal extraction method for extracting the genomic DNA from Clematis fasciculiflora Franch.[Method] Leavies of Clematis fasciculiflora Franch were used as materials for comparing the purity and concentration of extracted DNA and extracting time among the four extraction methods of genomic DNA including improved CTAB method Ⅰ,improved CTAB method Ⅱ,improved CTAB method Ⅲ and improved SDS method.[Result] The four extraction methods could all be successfully used for extracting the genomic DNA from Clematis fasciculiflora Franch.The purity of genomic DNA was the highest using improved CTAB method Ⅰ,with the longest extracting time;while the concentration of genomic DNA was the maximum using the improved SDS method,with the shortest extracting time and relatively low purity;the extracting time of improved CTAB method Ⅲ was the shortest.[Conclusion] This study had established the optimal extraction method for extracting the genomic DNA from Clematis fasciculiflora Franch and supported for the further research using molecular biological methods.
文摘目的通过研究杀青方式和时间、干燥方式对药用植物独蒜兰Pleione bulbocodioides假鳞茎外观、显微结构和化学成分含量的影响,探索其最佳初加工工艺。方法采用烘箱直接烘干(oven direct drying,OD)、煮制10、20、30 min后烘干(boiling for 10,20,30 min and then drying,B10D、B20D、B30D)、隔水蒸制10、20、30 min后烘干(steaming for 10,20,30 min and then drying,S10D、S20D、S30D)和冷冻干燥(freeze drying,FD)共8种方式处理独蒜兰假鳞茎,测定其2-O-葡萄糖基白及苷(dactylorhin A)、白及苷和山药素Ⅲ的含量。通过构建HPLC指纹图谱,并进行相似度分析(similarity analysis,SA)、层次聚类分析(hierarchical cluster analysis,HCA)和主成分分析(principal component analysis,PCA)评价加工方法对假鳞茎质量的影响。通过外观特征、组织结构及粉末显微结构观察,评估加工方法对假鳞茎结构的影响。以dactylorhin A、白及苷、山药素Ⅲ、醇溶性浸出物、水溶性浸出物、折干率、水分、灰分和酸不溶性灰分含量为指标,利用熵权法、层次分析法(analytic hierarchy process,AHP)和逼近理想解排序法(technique for order preference by similarity to ideal solution,TOPSIS)综合评价不同加工方法对假鳞茎品质的影响。结果8种加工方法所得样品的HPLC指纹图谱相似度为0.681~0.994。其中,FD样品的相似度最低,且HCA和PCA均将其单独归为一类,其余7种加工方法的样品则聚为另一类。通过HPLC指纹图谱24个共有峰鉴定出3个共有峰,分别为dactylorhin A(峰7)、白及苷(峰9)和山药素Ⅲ(峰15)。TOPSIS综合评价结果发现,S10D处理样品的贴近度以及dactylorhin A、白及苷和山药素Ⅲ总含量最高。结论隔水蒸制10 min后烘干是独蒜兰假鳞茎初加工的最优方法,该方法有利于活性成分保留,且工艺简便。为独蒜兰假鳞茎的规范化加工以及山慈菇药材质量标准的完善提供了重要依据。
