In the present experiment, Pavlovian fear conditioning was adopted to study the effects of different early rearing environments on fear conditioning in adult rats. Weaned rats were reared in three manipulable environm...In the present experiment, Pavlovian fear conditioning was adopted to study the effects of different early rearing environments on fear conditioning in adult rats. Weaned rats were reared in three manipulable environments （enriched, social and isolated conditions）. After 8 weeks, fear conditioning （characterized by percentage of freezing） was observed and analyzed, and rats＇ weight, locomoter activity and foot-shock sensitivity were operated too. The results showed that： （1） Compared with control group, the level of conditioned fear was significantly increased in enriched group, but significantly decreased in isolated group; （2） Enriched and isolated conditions influenced rat＇s weight significantly; （3） Different rearing conditions have no effect on locomoter activity and foot-shock sensitivity. These results indicated that early enriched condition could improve the tone-evoked fear conditioning response, while isolated condition impaired the response.展开更多
Key requirements of successful animal behavior research in the laboratory are robustness,objectivity,and high throughput,which apply to both the recording and analysis of behavior.Many automatic methods of monitoring ...Key requirements of successful animal behavior research in the laboratory are robustness,objectivity,and high throughput,which apply to both the recording and analysis of behavior.Many automatic methods of monitoring animal behavior meet these requirements.However,they usually depend on high-performing hardware and sophisticated software,which may be expensive.Here,we describe an automatic infrared behavior-monitor(AIBM)system based on an infrared touchscreen frame.Using this,animal positions can be recorded and used for further behavioral analysis by any PC supporting touch events.This system detects animal behavior in real time and gives closed-loop feedback using relatively low computing resources and simple algorithms.The AIBM system automatically records and analyzes multiple types of animal behavior in a highly efficient,unbiased,and low-cost manner.展开更多
基金National Natural Science Foundation of China(30770689)Scientific Research Fund of Hunan Provincial Education Department (05B045)National Basic Research Program of China (2002CB410803-04)
文摘In the present experiment, Pavlovian fear conditioning was adopted to study the effects of different early rearing environments on fear conditioning in adult rats. Weaned rats were reared in three manipulable environments （enriched, social and isolated conditions）. After 8 weeks, fear conditioning （characterized by percentage of freezing） was observed and analyzed, and rats＇ weight, locomoter activity and foot-shock sensitivity were operated too. The results showed that： （1） Compared with control group, the level of conditioned fear was significantly increased in enriched group, but significantly decreased in isolated group; （2） Enriched and isolated conditions influenced rat＇s weight significantly; （3） Different rearing conditions have no effect on locomoter activity and foot-shock sensitivity. These results indicated that early enriched condition could improve the tone-evoked fear conditioning response, while isolated condition impaired the response.
基金This work was supported by a Shenzhen Governmental Grant(JCYJ20180302145710934)the National Natural Science Foundation of China(31700907 and 31700908)+6 种基金the Key-Area Research and Development Program of Guangdong Province(2018B030331001)the International Partnership Program of the Chinese Academy of Sciences(172644KYS820170004)the Strategic Priority Research Program of Chinese Academy of Science(XDB32030100)Guangdong Special Support Program([2018]9)Ten Thousand Talent ProgramKey Laboratory of SIAT(2019DP173024)the Center for Excellence in Brain Science and Intelligence Technology,Chinese Academy of Sciences.
文摘Key requirements of successful animal behavior research in the laboratory are robustness,objectivity,and high throughput,which apply to both the recording and analysis of behavior.Many automatic methods of monitoring animal behavior meet these requirements.However,they usually depend on high-performing hardware and sophisticated software,which may be expensive.Here,we describe an automatic infrared behavior-monitor(AIBM)system based on an infrared touchscreen frame.Using this,animal positions can be recorded and used for further behavioral analysis by any PC supporting touch events.This system detects animal behavior in real time and gives closed-loop feedback using relatively low computing resources and simple algorithms.The AIBM system automatically records and analyzes multiple types of animal behavior in a highly efficient,unbiased,and low-cost manner.
基金supported by the National Natural Science Foundation of China(No.8127031681271922+6 种基金81470563)the Research Program of Soochow UniversityChina(No.Q413400111)Suzhou Science and Technology Development ProjectsChina(No.SYS201364SYS201258SYSD2012116)
