A doubled haploid （DH） population, which consists of 120 lines derived from anther culture of a typical indica and japonica hybrid‘CJ06＇/‘TNI＇, was used in this study. Ligule lengths of flag leaf were investigat...A doubled haploid （DH） population, which consists of 120 lines derived from anther culture of a typical indica and japonica hybrid‘CJ06＇/‘TNI＇, was used in this study. Ligule lengths of flag leaf were investigated for quantitative trait loci （QTL） mapping using the DH population. Five QTLs （qLL-2, qLL.4, qLL-6, qLL-IO and qLL-12） controlling the ligule length （LL） were detected on chromosomes 2, 4, 6, 10 and 12, with the variances explained 11.4%, 13.6%, 27.8%, 22.1% and 11.0%, respectively. Using four known genes of ZmGL1, ZmGL2, ZmGL3 and ZmGL4 in maize from the MaizeGDB, their homologs in rice were aligned and integrated into the existing simple sequence repeats linkage map by in silico mapping. A ZmLG1 homolog gene, OsLG1 encoding a squamosa promoter binding protein, was located between the markers RM255 and RM280, which is just identical to the interval of qLL.4 on the long arm of chromosome 4. The results are beneficial to dissection of the ligule molecular mechanism and the study of cereal evolution.展开更多
【目的】探究田间施硅对稻纵卷叶螟Cnaphalocrocis medinalis幼虫结苞危害的控制作用及其对水稻产量的影响。【方法】设置品种和施硅水平双因素田间小区试验,品种为易感虫的台中1号(Taichung Native 1,TN1)和抗虫的中浙优8号(Zhongzheyo...【目的】探究田间施硅对稻纵卷叶螟Cnaphalocrocis medinalis幼虫结苞危害的控制作用及其对水稻产量的影响。【方法】设置品种和施硅水平双因素田间小区试验,品种为易感虫的台中1号(Taichung Native 1,TN1)和抗虫的中浙优8号(Zhongzheyou 8,ZY8),施硅水平为0、150、300和600 kg SiO_(2)/hm^(2),测定稻纵卷叶螟幼虫在水稻分蘖期和孕穗期的结苞为害特征、孕穗期剑叶性状以及水稻产量相关指标。【结果】水稻成熟后土壤硅含量在品种间没有显著差异(P=0.910),但与不施硅对照相比,300和600 kg SiO_(2)/hm^(2)水平下土壤硅含量分别显著增加133.4%和182.4%(TN1,P≤0.030),以及154.9%和303.8%(ZY8,P≤0.005)。分蘖期水稻上稻纵卷叶螟叶苞的叶位和叶面分布特征在施硅水平之间有显著差异(P<0.001),施硅显著增大叶片正面结苞的频率(P<0.001)和叶苞丝束数量(P=0.020),分蘖期中浙优8号水稻上稻纵卷叶螟在叶片正面结苞的频率高于台中1号;不同施硅水平和品种下叶苞中的稻纵卷叶螟取食率和蛹重没有显著差异(P≥0.120)。孕穗期水稻上稻纵卷叶螟叶苞特征没有差异,但600kg SiO_(2)/hm^(2)水平硅处理显著降低幼虫取食率(P<0.001)、增加叶苞中丝束数量(P=0.002);剑叶理化性状受施硅影响,施硅显著降低剑叶的比叶面积、增加叶绿素相对含量和硅含量(P≤0.002)。此外,产量性状也与施硅有关,施硅显著增加中浙优8号的每穗总粒数、结实率和理论产量(P≤0.016)。不同施硅水平下,150 kg SiO_(2)/hm^(2)的理论收益最大。【结论】施硅能够改善水稻叶片的理化性质,增强叶片的韧性和光合作用,增强水稻对稻纵卷叶螟幼虫结苞为害的抵抗性和耐受性,并增加水稻理论产量,推荐田间施硅150 kg SiO_(2)/hm^(2)以获取较高收益。展开更多
基金Supported by the State Key Basic Research and Development Plan ofChina(2007CB10920203)the National Natural Science Foundation of China(30771160,30571136)+1 种基金the Open Fund of State Key Laboratory of RiceBiology(080104)the Research Program of Zhejiang Province.
文摘A doubled haploid （DH） population, which consists of 120 lines derived from anther culture of a typical indica and japonica hybrid‘CJ06＇/‘TNI＇, was used in this study. Ligule lengths of flag leaf were investigated for quantitative trait loci （QTL） mapping using the DH population. Five QTLs （qLL-2, qLL.4, qLL-6, qLL-IO and qLL-12） controlling the ligule length （LL） were detected on chromosomes 2, 4, 6, 10 and 12, with the variances explained 11.4%, 13.6%, 27.8%, 22.1% and 11.0%, respectively. Using four known genes of ZmGL1, ZmGL2, ZmGL3 and ZmGL4 in maize from the MaizeGDB, their homologs in rice were aligned and integrated into the existing simple sequence repeats linkage map by in silico mapping. A ZmLG1 homolog gene, OsLG1 encoding a squamosa promoter binding protein, was located between the markers RM255 and RM280, which is just identical to the interval of qLL.4 on the long arm of chromosome 4. The results are beneficial to dissection of the ligule molecular mechanism and the study of cereal evolution.
文摘【目的】探究田间施硅对稻纵卷叶螟Cnaphalocrocis medinalis幼虫结苞危害的控制作用及其对水稻产量的影响。【方法】设置品种和施硅水平双因素田间小区试验,品种为易感虫的台中1号(Taichung Native 1,TN1)和抗虫的中浙优8号(Zhongzheyou 8,ZY8),施硅水平为0、150、300和600 kg SiO_(2)/hm^(2),测定稻纵卷叶螟幼虫在水稻分蘖期和孕穗期的结苞为害特征、孕穗期剑叶性状以及水稻产量相关指标。【结果】水稻成熟后土壤硅含量在品种间没有显著差异(P=0.910),但与不施硅对照相比,300和600 kg SiO_(2)/hm^(2)水平下土壤硅含量分别显著增加133.4%和182.4%(TN1,P≤0.030),以及154.9%和303.8%(ZY8,P≤0.005)。分蘖期水稻上稻纵卷叶螟叶苞的叶位和叶面分布特征在施硅水平之间有显著差异(P<0.001),施硅显著增大叶片正面结苞的频率(P<0.001)和叶苞丝束数量(P=0.020),分蘖期中浙优8号水稻上稻纵卷叶螟在叶片正面结苞的频率高于台中1号;不同施硅水平和品种下叶苞中的稻纵卷叶螟取食率和蛹重没有显著差异(P≥0.120)。孕穗期水稻上稻纵卷叶螟叶苞特征没有差异,但600kg SiO_(2)/hm^(2)水平硅处理显著降低幼虫取食率(P<0.001)、增加叶苞中丝束数量(P=0.002);剑叶理化性状受施硅影响,施硅显著降低剑叶的比叶面积、增加叶绿素相对含量和硅含量(P≤0.002)。此外,产量性状也与施硅有关,施硅显著增加中浙优8号的每穗总粒数、结实率和理论产量(P≤0.016)。不同施硅水平下,150 kg SiO_(2)/hm^(2)的理论收益最大。【结论】施硅能够改善水稻叶片的理化性质,增强叶片的韧性和光合作用,增强水稻对稻纵卷叶螟幼虫结苞为害的抵抗性和耐受性,并增加水稻理论产量,推荐田间施硅150 kg SiO_(2)/hm^(2)以获取较高收益。
