目的:分析儿童体质测试成绩与粗大动作技能发展之间的关系,对不同儿童进行有针对性的指导和帮助。方法:北京市4所幼儿园共1 928名儿童,按照《国民体质测定标准手册》(幼儿部分)进行体质测试。随机整群选取了8个班作为整群对照(CG,n=244)...目的:分析儿童体质测试成绩与粗大动作技能发展之间的关系,对不同儿童进行有针对性的指导和帮助。方法:北京市4所幼儿园共1 928名儿童,按照《国民体质测定标准手册》(幼儿部分)进行体质测试。随机整群选取了8个班作为整群对照(CG,n=244),并根据体质测试成绩,选取成绩前10%的儿童(TG,n=203)和后10%儿童(BG,n=203),采用Test of Gross Motor Development-3(TGMD-3)对儿童粗大动作发展进行评估,对移动类和球类技能两大类基本动作的动作技能进行评估。结果:1)男童移动动作分值与女童无明显差别;2)男童球类动作技能好于女童,且随年龄增加其优势更加明显;3)体质测试成绩差的儿童,动作发展低于整体水平,特别是其球类动作技能的发展;4)随着年龄的增长,儿童动作发展的差距随年龄增长有逐渐扩大的趋势;5)粗大动作发展与体质测试结果中等相关。结论:针对3~6岁儿童,促进其体质发展,必须重视儿童动作技能的发展,对动作技能发展滞后的儿童应在教学活动中给予更多关注和指导。展开更多
AIM： To establish a novel coculture system for ex vivo expansion of umbilical cord blood（UCB） hematopoietic progenitors using thrombopoietin （TPO）/FIt-3 ligand （FL）-transduced human marrow-derived mesenchymal s...AIM： To establish a novel coculture system for ex vivo expansion of umbilical cord blood（UCB） hematopoietic progenitors using thrombopoietin （TPO）/FIt-3 ligand （FL）-transduced human marrow-derived mesenchymal stem cells （tfhMSCs） as feeder. METHODS： UCB CD34^＋ cells were isolated and cultured using four culture systems in serum-containing or serumfree medium. Suitable aliquots of cultured cells were used to monitor cell production, clonogenic activity, and long-term culture-initiating culture （LTC-IC） output. Finally, the severe-combined immunodeficient （SCID） mouse-repopulating cell （SRC） assay was performed to confirm ability of the cultured cells to reconstitute longterm hematopoiesis. RESULTS： There were no significant differences in the number of total nudeated cells among different culture systems in serum-containing medium during 21-d culture. However, on d 14, the outputs of CD34^＋ cells, CFU-C and CFU-GEMM in tfhMSCs coculture system were significantly enhanced. LTC-IC assay demonstrated that the tfhMSCs coculture system had the most powerful activity. The severe-combined immunodeficient （SCID） mouse repopulating cell （SRC） assay confirmed extensive ability of the expanded cells to reconstitute long-term hematopoiesis. Furthermore, PCR analysis demonstrated the presence of human hematopoietic cells in the bone marrow and peripheral blood cells of NOD/SCID mice.CONCLUSION： The TPO/FL-transduced hMSCs, in combination with additive cytokines, can effectively expand hematopoietic progenitors from UCB in vitro and the tfhMSCs coculture system may be a suitable system for ex vivo manipulation of primitive progenitor cells under contact culture conditions.展开更多
文摘目的:分析儿童体质测试成绩与粗大动作技能发展之间的关系,对不同儿童进行有针对性的指导和帮助。方法:北京市4所幼儿园共1 928名儿童,按照《国民体质测定标准手册》(幼儿部分)进行体质测试。随机整群选取了8个班作为整群对照(CG,n=244),并根据体质测试成绩,选取成绩前10%的儿童(TG,n=203)和后10%儿童(BG,n=203),采用Test of Gross Motor Development-3(TGMD-3)对儿童粗大动作发展进行评估,对移动类和球类技能两大类基本动作的动作技能进行评估。结果:1)男童移动动作分值与女童无明显差别;2)男童球类动作技能好于女童,且随年龄增加其优势更加明显;3)体质测试成绩差的儿童,动作发展低于整体水平,特别是其球类动作技能的发展;4)随着年龄的增长,儿童动作发展的差距随年龄增长有逐渐扩大的趋势;5)粗大动作发展与体质测试结果中等相关。结论:针对3~6岁儿童,促进其体质发展,必须重视儿童动作技能的发展,对动作技能发展滞后的儿童应在教学活动中给予更多关注和指导。
基金Supported by the grants of NIH-Heart,Lung & Blood,No.IR014L70593-01 and Zhejiang Scientific Foundation,No.2003C23015
文摘AIM： To establish a novel coculture system for ex vivo expansion of umbilical cord blood（UCB） hematopoietic progenitors using thrombopoietin （TPO）/FIt-3 ligand （FL）-transduced human marrow-derived mesenchymal stem cells （tfhMSCs） as feeder. METHODS： UCB CD34^＋ cells were isolated and cultured using four culture systems in serum-containing or serumfree medium. Suitable aliquots of cultured cells were used to monitor cell production, clonogenic activity, and long-term culture-initiating culture （LTC-IC） output. Finally, the severe-combined immunodeficient （SCID） mouse-repopulating cell （SRC） assay was performed to confirm ability of the cultured cells to reconstitute longterm hematopoiesis. RESULTS： There were no significant differences in the number of total nudeated cells among different culture systems in serum-containing medium during 21-d culture. However, on d 14, the outputs of CD34^＋ cells, CFU-C and CFU-GEMM in tfhMSCs coculture system were significantly enhanced. LTC-IC assay demonstrated that the tfhMSCs coculture system had the most powerful activity. The severe-combined immunodeficient （SCID） mouse repopulating cell （SRC） assay confirmed extensive ability of the expanded cells to reconstitute long-term hematopoiesis. Furthermore, PCR analysis demonstrated the presence of human hematopoietic cells in the bone marrow and peripheral blood cells of NOD/SCID mice.CONCLUSION： The TPO/FL-transduced hMSCs, in combination with additive cytokines, can effectively expand hematopoietic progenitors from UCB in vitro and the tfhMSCs coculture system may be a suitable system for ex vivo manipulation of primitive progenitor cells under contact culture conditions.
