Quality control and screening of active substances in traditional Chinese medicines have been performed using fingerprint analysis. The spectrum-effect relationship between chromatography fingerprints and efficacy of ...Quality control and screening of active substances in traditional Chinese medicines have been performed using fingerprint analysis. The spectrum-effect relationship between chromatography fingerprints and efficacy of herbal drugs is considered as a potentially useful method for determining active ingredients in complex mixtures. The study was designed to develop a method for determining the bioactive components of a compound Chinese medicine called Tiefeng based on spectrum-effect relationships between high-performance liquid chromatography （HPLC） fingerprints and anticoccidial activities. Four peaks of the established HPLC fingerprint indicate the main bioactive components of this medicine. In addition, pharrnacodynamic atlas was defined and used to assess the anticoccidial activity of Tiefeng from different sources for the first time. We found that the level of anticoccidial activity of Tiefeng was consistent with the degree of similarity between the pharmacodynamic atlas and chromatogram of any sample. Furthermore, effect of this medicine was related with the main active constituents, along with the origin and the harvesting time.展开更多
太赫兹分子指纹谱是一种非常有潜力的无标记检测方法,可以对微量甚至痕量样品进行检测。然而太赫兹波的波长远远大于待测分子的尺寸,导致波与痕量物质之间的相互作用较为微弱,需要额外结构来增强样品对电磁波的吸收。本文在金属基底上...太赫兹分子指纹谱是一种非常有潜力的无标记检测方法,可以对微量甚至痕量样品进行检测。然而太赫兹波的波长远远大于待测分子的尺寸,导致波与痕量物质之间的相互作用较为微弱,需要额外结构来增强样品对电磁波的吸收。本文在金属基底上构造了倒置的非对称介质光栅结构。该结构利用导模共振(Guided-mode resonance,GMR)和连续域束缚态(Bound state In Continuum,BIC)显著提升了薄膜样品的太赫兹吸收谱。该结构仅需测量反射吸收信号就可以得到薄膜增强吸收谱,而且样品涂覆于平坦的倒置的介质光栅背面,易于制备。当该结构用于0.2μm厚的α-乳糖薄膜时,吸收谱幅度增强达到236倍。该结构为太赫兹波段痕量分析物的检测提供了一种新的方法。展开更多
基金funded by the National Key Technology R&D Program, China (2008 BADB4B05-02)
文摘Quality control and screening of active substances in traditional Chinese medicines have been performed using fingerprint analysis. The spectrum-effect relationship between chromatography fingerprints and efficacy of herbal drugs is considered as a potentially useful method for determining active ingredients in complex mixtures. The study was designed to develop a method for determining the bioactive components of a compound Chinese medicine called Tiefeng based on spectrum-effect relationships between high-performance liquid chromatography （HPLC） fingerprints and anticoccidial activities. Four peaks of the established HPLC fingerprint indicate the main bioactive components of this medicine. In addition, pharrnacodynamic atlas was defined and used to assess the anticoccidial activity of Tiefeng from different sources for the first time. We found that the level of anticoccidial activity of Tiefeng was consistent with the degree of similarity between the pharmacodynamic atlas and chromatogram of any sample. Furthermore, effect of this medicine was related with the main active constituents, along with the origin and the harvesting time.
文摘太赫兹分子指纹谱是一种非常有潜力的无标记检测方法,可以对微量甚至痕量样品进行检测。然而太赫兹波的波长远远大于待测分子的尺寸,导致波与痕量物质之间的相互作用较为微弱,需要额外结构来增强样品对电磁波的吸收。本文在金属基底上构造了倒置的非对称介质光栅结构。该结构利用导模共振(Guided-mode resonance,GMR)和连续域束缚态(Bound state In Continuum,BIC)显著提升了薄膜样品的太赫兹吸收谱。该结构仅需测量反射吸收信号就可以得到薄膜增强吸收谱,而且样品涂覆于平坦的倒置的介质光栅背面,易于制备。当该结构用于0.2μm厚的α-乳糖薄膜时,吸收谱幅度增强达到236倍。该结构为太赫兹波段痕量分析物的检测提供了一种新的方法。
