摘要
目的:建立高效液相色谱法(HPLC)芍药甘草汤的指纹图谱和含量测定,并结合化学模式识别对其进行质量分析,筛选差异标志物。方法:采用HPLC法,以乙腈-0.1%磷酸为流动相,梯度洗脱,建立芍药甘草汤干膏粉的HPLC指纹图谱并同时测定30批芍药甘草汤中3种指标性成分,并应用相关性分析、聚类分析、主成分分析、正交偏最小二乘法判别分析等多元统计方法比较样品间的差异性,筛选差异标志物。结果:30批芍药甘草汤指纹图谱标定了18个共有峰,相似度0.924~0.996,并指认了10个色谱峰,分别为没食子酸、芍药内酯苷、芍药苷、芹糖甘草苷、甘草苷、1,2,3,4,6-五没食子酰葡萄糖(PGG)、没食子酰芍药苷、芹糖异甘草苷、异甘草苷、甘草酸;并以芍药苷、甘草苷、甘草酸为指标性成分,进行含量测定;化学模式识别分析将芍药甘草汤样品分成3类,筛选出10个色谱峰为质量标志物。结论:建立了芍药甘草汤指纹图谱,筛选出引起批次间差异的质量标志物。
Objective:To establish a high-performance liquid chromatography(HPLC) fingerprinting method for Shaoyao Gancao Decoction and its content determination,and to perform quality analysis combined with chemical pattern recognition to identify differential markers.Methods:HPLC was employed with acetonitrile and 0.1% phosphoric acid as the mobile phase and gradient elution.The HPLC fingerprint spectrum of Shaoyao Gancao Decoction dry extract powder was established,and three marker compounds were measured in 30 batches of Shaoyao Gancao Decoction.Multivariate statistical methods,including correlation analysis,cluster analysis,principal component analysis(PCA),and orthogonal partial least squares discriminant analysis(OPLS-DA),were applied to compare the differences between the samples and identify the differential markers.Results:The fingerprint spectrum of 30 batches of Shaoyao Gancao Decoction established 18 common peaks with similarity ranging from 0.924 to 0.996,and 10 peaks were identified as gallic acid,albiflorin,paeoniflorin,liquiritin apioside,liquiritin,1,2,3,4,6-pentyl-O-galloyl-beta-D-glucose(PGG),galloylpaeoniflorin,isoliquiritin apioside,isoliquiritin,and glycyrrhizic acid.Paeoniflorin,liquiritin,and glycyrrhizic acid were selected as the marker compounds for content determination.The chemical pattern recognition analysis classified the Shaoyao Gancao Decoction samples into three groups and identified 10 chromatographic peaks as quality markers.Conclusion:The HPLC fingerprint of Shaoyao Gancao Decoction was established,and quality markers responsible for the batch-to-batch differences were identified.
作者
余灵静
鲁方奇
陈诺
吴薇
张云羽
郑云枫
程建明
YU Lingjing;LU Fangnqi;CHEN Nuo;WU Wei;ZHANG Yunyu;ZHENG Yunfeng;CHENG Jianming(School of Pharmacy,Nanjing University of Chinese Medicine/Jiangsu Province Engineering Research Center of Classical Prescription,Nanjing 210023,China)
出处
《世界中医药》
北大核心
2025年第5期718-725,共8页
World Chinese Medicine
基金
国家自然科学基金项目(81973482)。
关键词
芍药甘草汤
指纹图谱
含量测定
聚类分析
主成分分析
正交偏最小二乘法判别分析
化学模式识别
质量标志物
Shaoyao Gancao Decoction
Fingerprint spectrum
Content determination
Cluster analysis
Principal component analysis
Orthogonal Partial Least Squares Discriminant Analysis
Chemical pattern recognition
Quality markers
