In this paper,a standardized analysis method is established for identifying meat quality-related genes in Ordos finewool sheep using transcriptome sequencing data.A meticulously standardized approach is utilized to in...In this paper,a standardized analysis method is established for identifying meat quality-related genes in Ordos finewool sheep using transcriptome sequencing data.A meticulously standardized approach is utilized to investigate the genetic determinants of meat quality in Ordos fine-wool sheep through transcriptome sequencing analysis.Muscle samples from the longissimus dorsi of one-year-old sheep are collected under controlled conditions,and key texture properties—hardness,elasticity,and chewiness—are measured to categorize samples into high-and low-textural-value groups.Genes significantly associated with meat quality traits are identified through standardized RNA extraction,high-throughput sequencing,and differential gene expression analysis.Functional enrichment analysis reveals their involvement in biological processes such as extracellular matrix organization and metabolic pathways.The findings underscore the pivotal role of standardization in meat quality research,laying a solid scientific foundation for future research on meat quality improvement and molecular breeding.展开更多
Background： Increasing the polyunsaturated fatty acid （PUFA） content and decreasing the saturated fatty acid （SFA） content of mutton can help to improve its nutritional value for consumers. Several laboratories h...Background： Increasing the polyunsaturated fatty acid （PUFA） content and decreasing the saturated fatty acid （SFA） content of mutton can help to improve its nutritional value for consumers. Several laboratories have evaluated the effects of vitamin E on the fatty acid （FA） composition of muscle in sheep. However, little information is available on wool sheep, even though wool sheep breeds are an important source of mutton, especially in northern China where sheep are extensively farmed. The present study was designed to address the effects of vitamin E on muscle FA composition in male Aohan fine-wool sheep. Methods： Forty-two male Aohan fine-wool lambs （5 mo old） with similar initial body weight were randomly divided into seven groups and fed diets supplemented with 0 （control group）, 20, 100, 200, 1,000, 2,000, or 2,400 IU/sheep/d vitamin E for 12 mo. Three lambs from each group were slaughtered to measure vitamin E and FA content in the Iongissimus lumborum （LL） and gluteus medius （GM） muscles. Results： Vitamin E concentrations in the LL and GM increased significantly after 12 mo of vitamin E supplementation （P 〈 0.05）. However, this increase did not occur in a dose-dependent manner because the muscle vitamin E concentration was highest in the 200 IU/sheep/d group. Dietary vitamin E supplementation also caused a significant reduction in SFA content and an increase in monounsaturated FA （MUFA） content in the LL and GM （P 〈 0.05）. All six doses of vitamin E significantly increased cis9 tronsl -conjugated linoleic acid （cgtl -CLA） content in the LL compared with the control group （P 〈 0.05）. Conclusions： Dietary supplementation with vitamin E increased muscle vitamin E content and improved the nutritional value of mutton by decreasing SFA content and increasing MUFA and c9tl 1-CLA contents in Aohan fine-wool sheep. These effects were greatest in sheep fed a diet containing 200 IU/sheep/d vitamin E.展开更多
Objective] This study aimed to estimate the heritability of Aohan fine-wool sheep＇s main economic characters. [ Method] Half-sib correlation in sire method was adopted to estimate Aohan file-wool sheep＇s Birth Weigh...Objective] This study aimed to estimate the heritability of Aohan fine-wool sheep＇s main economic characters. [ Method] Half-sib correlation in sire method was adopted to estimate Aohan file-wool sheep＇s Birth Weight（BW）, Weaning Litter Weight （WLW）, Weaning Litter Staple Fineness（WLSF）, One-age Staple Fineness（OASF）, One-age Staple Length （ OASL）, One-age Wool Yield（OAWY）, Main Economic Characters of One-age Aohan fine-wool sheep, Two-age Staple Fineness （TASF）, Two-age Staple Length （TASL）, Two-age Wool Yield （TAWY）, Two-age Weight （TAW） after cropping. [ Result] The heritability was 0.1 -0.7. [ Conclusion~ The heritability of Aohan fine-wool sheep＇s main characters was middle-and-high.展开更多
Five hybridomas stably secreting monoclonal antibodies (McAbs) to androstenedione were prepared by using artificially synthesized androstenedione-11α-succinyl conjugate with bovine serum albumin (BSA) as antigen. The...Five hybridomas stably secreting monoclonal antibodies (McAbs) to androstenedione were prepared by using artificially synthesized androstenedione-11α-succinyl conjugate with bovine serum albumin (BSA) as antigen. These McAbs showed slightly high cross-reactivity with testosterone (8.1—12.3%) and estrone (0.8—2.5%) and high affinities ranging from 2.0×10~7 to 2.8×10~8 L/M. They were all of the IgG_1 subclass. Xinjiang finewool ewes which were passively immunized with McAbs had higher circulating levels of progesterone (P) and luteinizing hormone (LH) than those of control ewes in two oestrous cycles and these changes led to increased ovulation rate and twin lambs born in young ewes.展开更多
基金funded by the 2023 Inner Mongolia Public Institution High-Level Talent Introduction Scientific Research Support Project,and the Ordos Municipal Science and Technology Major Special Project(Grant No.2022EEDSKJZDZX021).
文摘In this paper,a standardized analysis method is established for identifying meat quality-related genes in Ordos finewool sheep using transcriptome sequencing data.A meticulously standardized approach is utilized to investigate the genetic determinants of meat quality in Ordos fine-wool sheep through transcriptome sequencing analysis.Muscle samples from the longissimus dorsi of one-year-old sheep are collected under controlled conditions,and key texture properties—hardness,elasticity,and chewiness—are measured to categorize samples into high-and low-textural-value groups.Genes significantly associated with meat quality traits are identified through standardized RNA extraction,high-throughput sequencing,and differential gene expression analysis.Functional enrichment analysis reveals their involvement in biological processes such as extracellular matrix organization and metabolic pathways.The findings underscore the pivotal role of standardization in meat quality research,laying a solid scientific foundation for future research on meat quality improvement and molecular breeding.
基金financially supported by projects 200903060CARS-39 from China Agricultural Ministry
文摘Background： Increasing the polyunsaturated fatty acid （PUFA） content and decreasing the saturated fatty acid （SFA） content of mutton can help to improve its nutritional value for consumers. Several laboratories have evaluated the effects of vitamin E on the fatty acid （FA） composition of muscle in sheep. However, little information is available on wool sheep, even though wool sheep breeds are an important source of mutton, especially in northern China where sheep are extensively farmed. The present study was designed to address the effects of vitamin E on muscle FA composition in male Aohan fine-wool sheep. Methods： Forty-two male Aohan fine-wool lambs （5 mo old） with similar initial body weight were randomly divided into seven groups and fed diets supplemented with 0 （control group）, 20, 100, 200, 1,000, 2,000, or 2,400 IU/sheep/d vitamin E for 12 mo. Three lambs from each group were slaughtered to measure vitamin E and FA content in the Iongissimus lumborum （LL） and gluteus medius （GM） muscles. Results： Vitamin E concentrations in the LL and GM increased significantly after 12 mo of vitamin E supplementation （P 〈 0.05）. However, this increase did not occur in a dose-dependent manner because the muscle vitamin E concentration was highest in the 200 IU/sheep/d group. Dietary vitamin E supplementation also caused a significant reduction in SFA content and an increase in monounsaturated FA （MUFA） content in the LL and GM （P 〈 0.05）. All six doses of vitamin E significantly increased cis9 tronsl -conjugated linoleic acid （cgtl -CLA） content in the LL compared with the control group （P 〈 0.05）. Conclusions： Dietary supplementation with vitamin E increased muscle vitamin E content and improved the nutritional value of mutton by decreasing SFA content and increasing MUFA and c9tl 1-CLA contents in Aohan fine-wool sheep. These effects were greatest in sheep fed a diet containing 200 IU/sheep/d vitamin E.
基金supported by Key Projected Supported by Ministry of National Science and Technology and Agricultural Science and Technology Achievement Reformer Fund projects (04EFN211500036)
文摘Objective] This study aimed to estimate the heritability of Aohan fine-wool sheep＇s main economic characters. [ Method] Half-sib correlation in sire method was adopted to estimate Aohan file-wool sheep＇s Birth Weight（BW）, Weaning Litter Weight （WLW）, Weaning Litter Staple Fineness（WLSF）, One-age Staple Fineness（OASF）, One-age Staple Length （ OASL）, One-age Wool Yield（OAWY）, Main Economic Characters of One-age Aohan fine-wool sheep, Two-age Staple Fineness （TASF）, Two-age Staple Length （TASL）, Two-age Wool Yield （TAWY）, Two-age Weight （TAW） after cropping. [ Result] The heritability was 0.1 -0.7. [ Conclusion~ The heritability of Aohan fine-wool sheep＇s main characters was middle-and-high.
基金Project supported by one of the National Research Subjects in the Seventh Five-Year Science and Technology Plan and the National Natural Science Foundation of China
文摘Five hybridomas stably secreting monoclonal antibodies (McAbs) to androstenedione were prepared by using artificially synthesized androstenedione-11α-succinyl conjugate with bovine serum albumin (BSA) as antigen. These McAbs showed slightly high cross-reactivity with testosterone (8.1—12.3%) and estrone (0.8—2.5%) and high affinities ranging from 2.0×10~7 to 2.8×10~8 L/M. They were all of the IgG_1 subclass. Xinjiang finewool ewes which were passively immunized with McAbs had higher circulating levels of progesterone (P) and luteinizing hormone (LH) than those of control ewes in two oestrous cycles and these changes led to increased ovulation rate and twin lambs born in young ewes.
