To investigate the expression characteristics of sex related gene of FTZ-F 1 in the half-smooth tongue-sole （Cynoglossus semilaevis）, the homologue FTZ-F1 （hsFTZ-F1） full-length cDNA was isolated from the testis b...To investigate the expression characteristics of sex related gene of FTZ-F 1 in the half-smooth tongue-sole （Cynoglossus semilaevis）, the homologue FTZ-F1 （hsFTZ-F1） full-length cDNA was isolated from the testis by homologous cloning, and the cDNA included the open reading frame and a 66bp 5＇-UTR, along with a 1619bp 3＇-UTR, encoding a predicted 485 amino acid protein. Sequence, tissue distribution and phylogenic analyses of the FTZ-F1 showed that the hsFTZ-F1 belonged to SF-1/Ad4BP group. The hsFTZ-F1 transcripts were highly abundant in the gonads, kidneys, brain and head-kidneys, but weakly in other tissues. However, the expression level in the brain and head-kidney of female was highly abundant than in the male. The hsFTZ-F1 expression was highly abundant in the embryo than in the larvae, which suggested that the hsFTZ-F1 may be involved in the organogenesis in the tongue sole.展开更多
This study utilizes panel data from 293 Chinese cities at the prefecture level or above,spanning from 2013 to 2022,to empirically examine the net effects and underlying mechanisms of establishing pilot free trade zone...This study utilizes panel data from 293 Chinese cities at the prefecture level or above,spanning from 2013 to 2022,to empirically examine the net effects and underlying mechanisms of establishing pilot free trade zones(FTZs)on integration into the digital global value chain(DGVC).The results demonstrate that the establishment of pilot FTZs positively promotes integration into the DGVC and generates a spatial spillover effect.Compared to coastal areas and small-and medium-sized cities,pilot FTZs have a more significant impact on the integration of inland regions and large cities into the DGVC.Mechanism tests reveal that pilot FTZs drive integration into the DGVC primarily by promoting digital industry agglomeration and enhancing the technological content of exports.Furthermore,bilateral foreign direct investment and digital trade rules positively amplify the impact of pilot FTZs on integration into the DGVC.Therefore,this study recommends comprehensively deepening the development of pilot FTZs,consistently enhancing the technological content of exports,and proactively establishing a sound digital trade regulatory framework.These recommendations offer valuable insights for DGVC integration and the construction of a high-standard free trade network.展开更多
基金Supported by grants from State 863 High-Technology R&D Project of China(2006AA10A403)Shandong Genetic Improvement Key Project for Agricultural OrganismDoctor Initial Funding of Guangdong Ocean University(0712103)
文摘To investigate the expression characteristics of sex related gene of FTZ-F 1 in the half-smooth tongue-sole （Cynoglossus semilaevis）, the homologue FTZ-F1 （hsFTZ-F1） full-length cDNA was isolated from the testis by homologous cloning, and the cDNA included the open reading frame and a 66bp 5＇-UTR, along with a 1619bp 3＇-UTR, encoding a predicted 485 amino acid protein. Sequence, tissue distribution and phylogenic analyses of the FTZ-F1 showed that the hsFTZ-F1 belonged to SF-1/Ad4BP group. The hsFTZ-F1 transcripts were highly abundant in the gonads, kidneys, brain and head-kidneys, but weakly in other tissues. However, the expression level in the brain and head-kidney of female was highly abundant than in the male. The hsFTZ-F1 expression was highly abundant in the embryo than in the larvae, which suggested that the hsFTZ-F1 may be involved in the organogenesis in the tongue sole.
基金supported by the Key Project of the Hainan Province Marxist Theory Research and Development Program,titled“Research on the Opportunities and Challenges of the Digital Economy Partnership Agreement(DEPA)for the Construction of the Hainan Free Trade Port”(Grant Number:2023HNMGC02).
文摘This study utilizes panel data from 293 Chinese cities at the prefecture level or above,spanning from 2013 to 2022,to empirically examine the net effects and underlying mechanisms of establishing pilot free trade zones(FTZs)on integration into the digital global value chain(DGVC).The results demonstrate that the establishment of pilot FTZs positively promotes integration into the DGVC and generates a spatial spillover effect.Compared to coastal areas and small-and medium-sized cities,pilot FTZs have a more significant impact on the integration of inland regions and large cities into the DGVC.Mechanism tests reveal that pilot FTZs drive integration into the DGVC primarily by promoting digital industry agglomeration and enhancing the technological content of exports.Furthermore,bilateral foreign direct investment and digital trade rules positively amplify the impact of pilot FTZs on integration into the DGVC.Therefore,this study recommends comprehensively deepening the development of pilot FTZs,consistently enhancing the technological content of exports,and proactively establishing a sound digital trade regulatory framework.These recommendations offer valuable insights for DGVC integration and the construction of a high-standard free trade network.
