Selenium(Se)is an essential micronutrient for lives.Indigenous microbial communities play an important role on Se geochemistry in soils.In this study,the microbial community composition and functions of 53 soil sample...Selenium(Se)is an essential micronutrient for lives.Indigenous microbial communities play an important role on Se geochemistry in soils.In this study,the microbial community composition and functions of 53 soil samples were investigated using high-throughput sequencing.Samples were divided into 3 groups with different farming types based on the measured geochemical parameters and microbial functional structures.Results indicated that putative Se related bacteria Bacillus,Dyella,Paenibacillus,Burkholderia and Brevibacillus were dominant in dryland plantation soils which were characterized with higher available Se and low contents of H_(2)O,total organic carbon(TOC),NH_(4)^(+) and NO_(2)^(-).In contrast,the putative denitrifier Pseudomonas dominated in flooded paddy soils with higher TOC,NO_(3)^(-) and organic Se,whereas genera Rhizobium,Nitrosospira,and Geobacter preferred woodland soils with higher oxidation-reduction potential(ORP),pH,NH_(4)^(+) and Fe.Farming patterns resulted in distinct geochemical parameters including moisture,pH,ORP,TOC,and contents of soluble Fe,NO_(2)^(-) and NH_(4)^(+),shaping the microbial communities,which in turn affected Se forms in soils.This study provides a valuable insight into understanding of Se biogeochemistry in soils and prospective strategy for Se-rich agriculture production.展开更多
P.frumentum biomass could be improved by appropriating returning measures.P.frumentum biomass was excellent in 75%alfalfa returning amount.Key species of bacteria differed among the alfalfa returning amountsThe relati...P.frumentum biomass could be improved by appropriating returning measures.P.frumentum biomass was excellent in 75%alfalfa returning amount.Key species of bacteria differed among the alfalfa returning amountsThe relationship of core bacteria and their potential ecological functions are more close to biomass.The use of green manure returning to field is a common practice in conservation tillage.However,there is limited research on how different amounts of alfalfa can affect saline-alkali soil properties,bacterial community characteristics,and subsequent productivity.In this study,five different amounts of alfalfa return were investigated to understand the biological relationships between rhizospheres soil properties,bacterial communities,potential functions,and the Purus frumentum biomass.The results showed that the biomass was highest when 75%of the alfalfa was returned to the field.This particular amount was associated with relatively low soil pH and electrical conductivity.Additionally,it increased the relative abundance of beneficial bacterial taxa in both core and non-core bacteria.Statistical analysis revealed significant differences in both core(RANOSIM=0.871,P=0.001)and non-core(RANOSIM=0.947,P=0.001)bacterial communities among the different amounts of alfalfa return based on non-metric multidimensional scaling analysis.Core bacterial taxa and their potential ecological functions were more closely related to plant biomass compared to non-core bacteria based on correlation analysis and multiple regression analysis.Therefore,our results indicate that optimizing the amount of alfalfa return can improve subsequent plant biomass.Regulating soil physicochemical properties and influencing core microbial community structure are of great significance for soil functional stability and crop productivity sustainability.展开更多
基金supported by the National Natural Science Foundation of China(No.41772260)the Science and Technology Programs of Department of Natural Resources of Zhejiang Province,China(No.2020006)the Open Project of Technology Innovation Center for Ecological Evaluation and Remediation of Agricultural Land in Plain Area,MNR(No.ZJGCJ202001)。
文摘Selenium(Se)is an essential micronutrient for lives.Indigenous microbial communities play an important role on Se geochemistry in soils.In this study,the microbial community composition and functions of 53 soil samples were investigated using high-throughput sequencing.Samples were divided into 3 groups with different farming types based on the measured geochemical parameters and microbial functional structures.Results indicated that putative Se related bacteria Bacillus,Dyella,Paenibacillus,Burkholderia and Brevibacillus were dominant in dryland plantation soils which were characterized with higher available Se and low contents of H_(2)O,total organic carbon(TOC),NH_(4)^(+) and NO_(2)^(-).In contrast,the putative denitrifier Pseudomonas dominated in flooded paddy soils with higher TOC,NO_(3)^(-) and organic Se,whereas genera Rhizobium,Nitrosospira,and Geobacter preferred woodland soils with higher oxidation-reduction potential(ORP),pH,NH_(4)^(+) and Fe.Farming patterns resulted in distinct geochemical parameters including moisture,pH,ORP,TOC,and contents of soluble Fe,NO_(2)^(-) and NH_(4)^(+),shaping the microbial communities,which in turn affected Se forms in soils.This study provides a valuable insight into understanding of Se biogeochemistry in soils and prospective strategy for Se-rich agriculture production.
基金financially supported by the Natural Science Foundation of Jiangsu Province(Grant No.BK20221513)the Key Research and Development Plan of Jiangsu Province(Grant No.BE2021365)the Postgraduate Research and Practice Innovation Program of Jiangsu Province(Grant No.SJCX23_0207).
文摘P.frumentum biomass could be improved by appropriating returning measures.P.frumentum biomass was excellent in 75%alfalfa returning amount.Key species of bacteria differed among the alfalfa returning amountsThe relationship of core bacteria and their potential ecological functions are more close to biomass.The use of green manure returning to field is a common practice in conservation tillage.However,there is limited research on how different amounts of alfalfa can affect saline-alkali soil properties,bacterial community characteristics,and subsequent productivity.In this study,five different amounts of alfalfa return were investigated to understand the biological relationships between rhizospheres soil properties,bacterial communities,potential functions,and the Purus frumentum biomass.The results showed that the biomass was highest when 75%of the alfalfa was returned to the field.This particular amount was associated with relatively low soil pH and electrical conductivity.Additionally,it increased the relative abundance of beneficial bacterial taxa in both core and non-core bacteria.Statistical analysis revealed significant differences in both core(RANOSIM=0.871,P=0.001)and non-core(RANOSIM=0.947,P=0.001)bacterial communities among the different amounts of alfalfa return based on non-metric multidimensional scaling analysis.Core bacterial taxa and their potential ecological functions were more closely related to plant biomass compared to non-core bacteria based on correlation analysis and multiple regression analysis.Therefore,our results indicate that optimizing the amount of alfalfa return can improve subsequent plant biomass.Regulating soil physicochemical properties and influencing core microbial community structure are of great significance for soil functional stability and crop productivity sustainability.
