This paper proposes the assumption of E-commerce platform of the agricultural products based on the F2C2B mode. The whole e-commerce model is guided by the idea of the supply chain management from the perspective of t...This paper proposes the assumption of E-commerce platform of the agricultural products based on the F2C2B mode. The whole e-commerce model is guided by the idea of the supply chain management from the perspective of the modern system, and the e-commerce integration model from customer to supplier is implemented, and the whole is optimized. E-commerce to cloud computing as the basic environment, the construction of public cloud services and private cloud resources, based on public cloud to the SaaS way to provide services to customers, focus on business search and business collaboration, make full use of the lnternet, modern communications technology to provide the real- service. The development of agricultural economy, no longer depends only on the number of some traditional agricultural resources, but also depends on the modern technology, information access and use. Through the development of basic network technology, by virtue of some modern electronic information technology and some other means that can improve the intelligence of agricultural use of the information resources, the proposed model provides the new methodology of the solution.展开更多
Objective:To investigate the expression of cyclooxygenase-2 and its pathological effect in the experimental nonalcoholic fatty liver of rats, and to explore its possible mechanism. Methods:The rat NAFLD model was es...Objective:To investigate the expression of cyclooxygenase-2 and its pathological effect in the experimental nonalcoholic fatty liver of rats, and to explore its possible mechanism. Methods:The rat NAFLD model was established by giving a fat-enriched diet. The blood samples were obtained form abdominal aorta and the levels of serum ALT, AST and IL-1, changes in the hepatic tissue 6-k-PGF1 α TXB2 were measured. The expression level of COX-2 in rats livers were assayed by immunohistochemistry, RT-PCR and Westernblot. Results: Light microscope analysis revealed that hepatocytes were injured in the model group and slightly in the treatment group. The levels of serum TXB2 and IL-1 in the fatty liver rats were increased. Compared with the model group, the IL-1 and TXB2 increased significantly(P〈 0.05), on the contrary, compared with the normal group, the hepatic tissue 6-Keto-prostagland decreased significantly in the model group(P 〈 0.05), the treatment group also increased but P 〉 0.05. There was no positive expression of COX-2 in hepatic tissue of normal rats. In the model group, there was positive expression of COX-2 antigen and the number of COX positive cells progressively increased at 4, 8, 12 wks. The intensity of expression of COX-2 had significantly increased(P 〈 0.05 ) and the intensity of COX-2 expression in the treated group decreased remarkably compared with the model group(P 〈 0.05). The expression of COX-2 mRNA and the level of COX-2 protein were significantly stronger in the liver of model rats compared with normal rats, and significantly weaker in treated rats, than in 8W and 12W model rats(P 〈 0.05). Conclusion:The increase of COX-2 expression in NAFLD is closely associated with the severity of liver inflammation and damage. COX-2 may play an important role in the progression of rat NAFLD, and the expression of COX-2 mRNA is downregulated by cyclooxygenase-2 inhibitor, which can depress the oxidative stress and control inflammatory response efficiently.展开更多
This study deals with Peak of electron density in F2-layer sensibility scale during quiet time on solar minimum. Peaks of electron density in F2-layer (NmF2) values at the quietest days are compared to those carried o...This study deals with Peak of electron density in F2-layer sensibility scale during quiet time on solar minimum. Peaks of electron density in F2-layer (NmF2) values at the quietest days are compared to those carried out from the two nearest days (previous and following of quietest day). The study uses International Reference Ionosphere (IRI) for ionosphere modeling. The located station is Ouagadougou, in West Africa. Solar minimum of phase 22 is considered in this study. Using three core principles of ionosphere modeling under IRI running conditions, the study enables to carry out Peak of electron density in F2-layer values during the quietest days of the characteristic months for the four different seasons. These parameters are compared to those of the previous and the following of the quietest days (the day before and following each quietest selected day) at the same hour. The knowledge of NmF2 values at the quietest days and at the two nearest days enables to calculate the relative error that can be made on this parameter. This calculation highlights insignificant relative errors. This means that NmF2 values at the two nearest days of each quietest day on solar minimum can be used for simulating the quietest days’ behavior. NmF2 values obtained by running IRI model have good correlation with those carried out by Thermosphere-Ionosphere-Electrodynamics-General Circulation Model (TIEGCM).展开更多
The single chain antibody scFv2F3 can be converted into selenium-containing Se-scFv2F3 by chemical mutation of the Ser residues. With antibody fragment 1NQB as a template, the catalytic domain of scFv2F3 was built by ...The single chain antibody scFv2F3 can be converted into selenium-containing Se-scFv2F3 by chemical mutation of the Ser residues. With antibody fragment 1NQB as a template, the catalytic domain of scFv2F3 was built by using homology modeling and molecular dynamics(MD) simulations. On the basis of the 3D model, we discussed the importance of Ser52 as the chemical modification site and redesigned the protein groups nearby Ser52 via intro- ducing a catalytic triad. The following 10 ns MD results show that the designed Ser52-Trp29-Gln72 catalytic triad is stable enough and high close to the local structural features of native glutathione peroxidases(GPX). Our results may be useful for creating a new abzyme with higher catalytic efficiency and stability.展开更多
Ionosphere layer is the atmosphere region which reflects radio waves for telecommunication. The density in particles in this layer influences the quality of communication. This study deals with the effects of Total El...Ionosphere layer is the atmosphere region which reflects radio waves for telecommunication. The density in particles in this layer influences the quality of communication. This study deals with the effects of Total Electron Contents (TEC) on the critical frequency of radio waves in the F2-layer. Total Electron Contents parameter symbolizes electron bulk surface density in ionosphere layer. Above critical frequency value in F2 layer (foF2), radio waves pass through ionosphere. The knowledge of this value enables to calibrate transmission frequencies. In this study, we consider TEC effects on foF2 under quiet time conditions during the maximum and the minimum of solar cycle 22, at Ouagadougou station, in West Africa. The study also considers the effects of seasons and the hourly variability of TEC and foF2. This work shows winter anomaly on foF2 and TEC on minimum and maximum of solar cycle phase respectively. Running International Reference Ionosphere (IRI) model enables to carry out the effects of TEC on foF2 by use of their monthly average values. This leads to a new approach to calibrate radio transmitters.展开更多
BACKGROUND: Pharmacological experiments have demonstrated that gastrodin has a protective effect on neonatal rat brain subjected to hypoxia-ischemia; however, the underlying mechanism has not been fully elucidated. O...BACKGROUND: Pharmacological experiments have demonstrated that gastrodin has a protective effect on neonatal rat brain subjected to hypoxia-ischemia; however, the underlying mechanism has not been fully elucidated. OBJECTIVE: The aim of this study was to investigate the acute therapeutic effects of gastrodin by observing prostaglandin B2 and 6-keto-prostaglandin F 1 a in brain tissue of neonatal rats that received gastrodin injections immediately after hypoxia-ischemia. DESIGN: Single-factor design. SETTING: Department of Pediatrics, Affiliated Hospital of Yanbian University. MATERIALS: This study was performed in the Laboratory of the Department of Pediatrics, Affiliated Hospital of Yanbian University (key laboratory of provincial Health Department) from April to December 2003. Fifty-five Wistar rats of either gender, aged 7 days, were provided by the Laboratory Animal Center of Affiliated Hospital of Yanbian University. The rats were randomly divided into normal control (n =10), model (n = 15), gastrodin-treated (n = 15), and Danshen-treated (n = 15) groups. The protocol was performed in accordance with guidelines from the Institute of Health Sciences for the use and care of animals. The following reagents were. used: Gastrodin (Sancai Medicine Group Co., Ltd., Zhongshan, Guangdong Province, China; component: gastrodin), Danshen (Conba Stock Company, Jinhua, Zhengjiang Province, China; component: salvia miltiorrhiza), and reagent kits for ^125I-prostaglandin B2 and ^125I-6-prostaglandin F l a (Research and Development Center for Science and Technology, General Hospital of Chinese PLA). METHODS: Rats in the normal control group received no treatment. Rats in the remaining 3 groups were anesthetized, followed by ligation of the left common carotid artery. One hour later, the rats were placed in a closed hypoxic box and allowed to inhale 8% oxygen-air (2.0 3.0 L/min) for 2 hours to develop hypoxic-ischemic encephalopathy. Immediately after lesion, rats in the gastrodin and Danshen-treated groups were intraperitoneally injected with l g gastrodin (10 mL/kg) and 15 g Danshen (l 5 mL/kg), respectively. MAIN OUTCOME MEASURES: Forty-eight hours after lesion, the left brain hemisphere was removed and homogenized to test the levels of prostaglandin B2 and 6-keto-prostaglandin F 1 a by radioimmunoassay. RESULTS: Forty successfully lesioned neonatal rats from the model, gastrodin-treated, and Danshen-treated groups, and ten rats from the control group, were included in the final analysis. Levels of prostaglandin B2 and 6-keto-prostaglandin F 1 a in brain tissue of neonatal rats were significantly higher in the model group than in the control group (both P 〈 0.01). Levels of prostaglandin B2 and 6-keto-prostaglandin F 1 a were significantly lower in the gastrodin-treated and the Danshen-treated groups compared to the model group (all P 〈 0.01). However, there were no significant differences in levels of prostaglandin B2 and 6-keto-prostaglandin F 1 a between the gastrodin-treated and the Danshen-treated groups (P 〉 0.05). CONCLUSION: Gastrodin decreased prostaglandin and thromboxan levels in neonatal rat brains subjected to hypoxia-ischemia.展开更多
A model for the negative phase of ionospheric storms in middle latitudes is presented. It is assumed that there will be molecule enriched air in the thermosphere above the auroral oval during the period of the main ph...A model for the negative phase of ionospheric storms in middle latitudes is presented. It is assumed that there will be molecule enriched air in the thermosphere above the auroral oval during the period of the main phase of a magnetic storm. The molecule enriched air is carried to the middle latitudes by thermospheric neutral wind, and at the same time it diffuses away. When the molecule enriched air arrives at the F2 layer above a station, the electron loss rate in the F2 layer increases, the electron density decreases and then the negative phase at the station begins. We have calculated the variations of the fo F2 following magnetic storms for Manzhouli (29.5°N, 117.5°E), Freiburg (48°N, 07°E) and Billerica (43°N, 71°W) respectively. The results agree very well with typical events observed at the three stations and can be used to explain some average features of negative phase ionospheric storms in middle latitudes.展开更多
A partially varying-coefficient model is one of the useful modelling tools.In this model, some coefficients of a linear model are kept to be constant whilst the others areallowed to vary with another factor. However, ...A partially varying-coefficient model is one of the useful modelling tools.In this model, some coefficients of a linear model are kept to be constant whilst the others areallowed to vary with another factor. However, rarely can the analysts know a priori whichcoefficients can be assumed to be constant and which ones are varying with the given factor.Therefore, the identification problem of the constant coefficients should be solved before thepartially varying-coefficient model is used to analyze a real-world data set. In this article, asimple test method is proposed to achieve this task, in which the test statistic is constructed asthe sample variance of the estimates of each coefficient function in a well-knownvarying-coefficient model. Moreover two procedures, called F-approximation and three-moment χ~2approximation, are employed to derive the p-value of the test. Furthermore, some simulations areconducted to examine the performance of the test and the results are satisfactory.展开更多
蚀变分带和成矿机制的准确厘定是建立斑岩成矿模型与找矿预测的关键。本文以新生代金沙江-哀牢山成矿带的玉龙斑岩铜矿为例,通过质量作用定律(LMA)和吉布斯自由能最小化模型(GEM),构建含矿热液与斑岩侵入体的pH-f O 2相图和动态传输模型...蚀变分带和成矿机制的准确厘定是建立斑岩成矿模型与找矿预测的关键。本文以新生代金沙江-哀牢山成矿带的玉龙斑岩铜矿为例,通过质量作用定律(LMA)和吉布斯自由能最小化模型(GEM),构建含矿热液与斑岩侵入体的pH-f O 2相图和动态传输模型,以揭示蚀变分带成因和金属成矿机制。LMA与GEM结果显示初始成矿流体pH值为4.7,logf_(O2)=-23.0(ΔFMQ=+2.7),且溶解Cu含量为1138×10^(-6),Mo为1.2×10^(-6)。研究表明,当该酸性及强氧化性流体流入二长花岗斑岩体时,在温度为450~360℃范围内,代表钾硅酸盐化蚀变的钾长石、黑云母、硬石膏、赤铁矿和磁铁矿的矿物逐渐沉淀,且与钾硅酸盐化蚀变相关流体具有较高pH值(5.0~7.0)和氧逸度(ΔFMQ=+2.9~+3.6)特征;当温度在360~320℃范围时,代表青磐岩化蚀变阶段的典型矿物如绿帘石、铁绿泥石和斜绿泥石等逐渐形成,流体pH值(5.0~6.4)和氧逸度(ΔFMQ=+1.1)均有所下降;当温度进一步从320℃下降到200℃时,流体pH值(5.0~5.7)进一步小幅下降,而氧逸度则(ΔFMQ=+1.7)略有回升,在此期间,绢云母和方解石等开始沉淀并形成典型的绢英岩化蚀变。此外,以HMoO_(4)^(-)和MoO_(4)^(2-)为载体的Mo在狭窄高温区间(450~370℃)内沉淀,而以CuCl(CuCl_(4)^(3-)、CuCl_(2)^(-)、CuCl)为主要载体的Cu则在在中、高温(450~300℃)范围中沉淀。通过利用LMA反演及GEM正演相结合定量化地刻画了玉龙斑岩铜矿水岩反应过程,由此揭示了斑岩矿床蚀变分带是逐渐冷却的单一岩浆热液与斑岩体不断反应的结果,且不同温度窗口对应着钾硅酸盐化(450~360℃)、青磐岩化(360~320℃)和绢英岩化(320~200℃)蚀变矿物的形成,故含矿流体温度的快速下降可能是玉龙铜矿蚀变叠加的重要因素。此外,Cu、Mo络合离子溶解度对温度变化的差异响应,导致了Mo矿化主要发育于靠近斑岩体的高温区域,而Cu则以网脉状-浸染状叠加到Mo矿化之上,并广泛分布于斑岩体周边的高-中温区域。展开更多
文摘This paper proposes the assumption of E-commerce platform of the agricultural products based on the F2C2B mode. The whole e-commerce model is guided by the idea of the supply chain management from the perspective of the modern system, and the e-commerce integration model from customer to supplier is implemented, and the whole is optimized. E-commerce to cloud computing as the basic environment, the construction of public cloud services and private cloud resources, based on public cloud to the SaaS way to provide services to customers, focus on business search and business collaboration, make full use of the lnternet, modern communications technology to provide the real- service. The development of agricultural economy, no longer depends only on the number of some traditional agricultural resources, but also depends on the modern technology, information access and use. Through the development of basic network technology, by virtue of some modern electronic information technology and some other means that can improve the intelligence of agricultural use of the information resources, the proposed model provides the new methodology of the solution.
文摘Objective:To investigate the expression of cyclooxygenase-2 and its pathological effect in the experimental nonalcoholic fatty liver of rats, and to explore its possible mechanism. Methods:The rat NAFLD model was established by giving a fat-enriched diet. The blood samples were obtained form abdominal aorta and the levels of serum ALT, AST and IL-1, changes in the hepatic tissue 6-k-PGF1 α TXB2 were measured. The expression level of COX-2 in rats livers were assayed by immunohistochemistry, RT-PCR and Westernblot. Results: Light microscope analysis revealed that hepatocytes were injured in the model group and slightly in the treatment group. The levels of serum TXB2 and IL-1 in the fatty liver rats were increased. Compared with the model group, the IL-1 and TXB2 increased significantly(P〈 0.05), on the contrary, compared with the normal group, the hepatic tissue 6-Keto-prostagland decreased significantly in the model group(P 〈 0.05), the treatment group also increased but P 〉 0.05. There was no positive expression of COX-2 in hepatic tissue of normal rats. In the model group, there was positive expression of COX-2 antigen and the number of COX positive cells progressively increased at 4, 8, 12 wks. The intensity of expression of COX-2 had significantly increased(P 〈 0.05 ) and the intensity of COX-2 expression in the treated group decreased remarkably compared with the model group(P 〈 0.05). The expression of COX-2 mRNA and the level of COX-2 protein were significantly stronger in the liver of model rats compared with normal rats, and significantly weaker in treated rats, than in 8W and 12W model rats(P 〈 0.05). Conclusion:The increase of COX-2 expression in NAFLD is closely associated with the severity of liver inflammation and damage. COX-2 may play an important role in the progression of rat NAFLD, and the expression of COX-2 mRNA is downregulated by cyclooxygenase-2 inhibitor, which can depress the oxidative stress and control inflammatory response efficiently.
文摘This study deals with Peak of electron density in F2-layer sensibility scale during quiet time on solar minimum. Peaks of electron density in F2-layer (NmF2) values at the quietest days are compared to those carried out from the two nearest days (previous and following of quietest day). The study uses International Reference Ionosphere (IRI) for ionosphere modeling. The located station is Ouagadougou, in West Africa. Solar minimum of phase 22 is considered in this study. Using three core principles of ionosphere modeling under IRI running conditions, the study enables to carry out Peak of electron density in F2-layer values during the quietest days of the characteristic months for the four different seasons. These parameters are compared to those of the previous and the following of the quietest days (the day before and following each quietest selected day) at the same hour. The knowledge of NmF2 values at the quietest days and at the two nearest days enables to calculate the relative error that can be made on this parameter. This calculation highlights insignificant relative errors. This means that NmF2 values at the two nearest days of each quietest day on solar minimum can be used for simulating the quietest days’ behavior. NmF2 values obtained by running IRI model have good correlation with those carried out by Thermosphere-Ionosphere-Electrodynamics-General Circulation Model (TIEGCM).
基金Supported by the National Natural Science Foundation of China(Nos.20333050,20673044)Program for Changjiang Scho-lars and Innovative Research Team in University of China(No.IRT0625) Key Subject of Science and Technology by Jilin Province,China
文摘The single chain antibody scFv2F3 can be converted into selenium-containing Se-scFv2F3 by chemical mutation of the Ser residues. With antibody fragment 1NQB as a template, the catalytic domain of scFv2F3 was built by using homology modeling and molecular dynamics(MD) simulations. On the basis of the 3D model, we discussed the importance of Ser52 as the chemical modification site and redesigned the protein groups nearby Ser52 via intro- ducing a catalytic triad. The following 10 ns MD results show that the designed Ser52-Trp29-Gln72 catalytic triad is stable enough and high close to the local structural features of native glutathione peroxidases(GPX). Our results may be useful for creating a new abzyme with higher catalytic efficiency and stability.
文摘Ionosphere layer is the atmosphere region which reflects radio waves for telecommunication. The density in particles in this layer influences the quality of communication. This study deals with the effects of Total Electron Contents (TEC) on the critical frequency of radio waves in the F2-layer. Total Electron Contents parameter symbolizes electron bulk surface density in ionosphere layer. Above critical frequency value in F2 layer (foF2), radio waves pass through ionosphere. The knowledge of this value enables to calibrate transmission frequencies. In this study, we consider TEC effects on foF2 under quiet time conditions during the maximum and the minimum of solar cycle 22, at Ouagadougou station, in West Africa. The study also considers the effects of seasons and the hourly variability of TEC and foF2. This work shows winter anomaly on foF2 and TEC on minimum and maximum of solar cycle phase respectively. Running International Reference Ionosphere (IRI) model enables to carry out the effects of TEC on foF2 by use of their monthly average values. This leads to a new approach to calibrate radio transmitters.
文摘BACKGROUND: Pharmacological experiments have demonstrated that gastrodin has a protective effect on neonatal rat brain subjected to hypoxia-ischemia; however, the underlying mechanism has not been fully elucidated. OBJECTIVE: The aim of this study was to investigate the acute therapeutic effects of gastrodin by observing prostaglandin B2 and 6-keto-prostaglandin F 1 a in brain tissue of neonatal rats that received gastrodin injections immediately after hypoxia-ischemia. DESIGN: Single-factor design. SETTING: Department of Pediatrics, Affiliated Hospital of Yanbian University. MATERIALS: This study was performed in the Laboratory of the Department of Pediatrics, Affiliated Hospital of Yanbian University (key laboratory of provincial Health Department) from April to December 2003. Fifty-five Wistar rats of either gender, aged 7 days, were provided by the Laboratory Animal Center of Affiliated Hospital of Yanbian University. The rats were randomly divided into normal control (n =10), model (n = 15), gastrodin-treated (n = 15), and Danshen-treated (n = 15) groups. The protocol was performed in accordance with guidelines from the Institute of Health Sciences for the use and care of animals. The following reagents were. used: Gastrodin (Sancai Medicine Group Co., Ltd., Zhongshan, Guangdong Province, China; component: gastrodin), Danshen (Conba Stock Company, Jinhua, Zhengjiang Province, China; component: salvia miltiorrhiza), and reagent kits for ^125I-prostaglandin B2 and ^125I-6-prostaglandin F l a (Research and Development Center for Science and Technology, General Hospital of Chinese PLA). METHODS: Rats in the normal control group received no treatment. Rats in the remaining 3 groups were anesthetized, followed by ligation of the left common carotid artery. One hour later, the rats were placed in a closed hypoxic box and allowed to inhale 8% oxygen-air (2.0 3.0 L/min) for 2 hours to develop hypoxic-ischemic encephalopathy. Immediately after lesion, rats in the gastrodin and Danshen-treated groups were intraperitoneally injected with l g gastrodin (10 mL/kg) and 15 g Danshen (l 5 mL/kg), respectively. MAIN OUTCOME MEASURES: Forty-eight hours after lesion, the left brain hemisphere was removed and homogenized to test the levels of prostaglandin B2 and 6-keto-prostaglandin F 1 a by radioimmunoassay. RESULTS: Forty successfully lesioned neonatal rats from the model, gastrodin-treated, and Danshen-treated groups, and ten rats from the control group, were included in the final analysis. Levels of prostaglandin B2 and 6-keto-prostaglandin F 1 a in brain tissue of neonatal rats were significantly higher in the model group than in the control group (both P 〈 0.01). Levels of prostaglandin B2 and 6-keto-prostaglandin F 1 a were significantly lower in the gastrodin-treated and the Danshen-treated groups compared to the model group (all P 〈 0.01). However, there were no significant differences in levels of prostaglandin B2 and 6-keto-prostaglandin F 1 a between the gastrodin-treated and the Danshen-treated groups (P 〉 0.05). CONCLUSION: Gastrodin decreased prostaglandin and thromboxan levels in neonatal rat brains subjected to hypoxia-ischemia.
文摘A model for the negative phase of ionospheric storms in middle latitudes is presented. It is assumed that there will be molecule enriched air in the thermosphere above the auroral oval during the period of the main phase of a magnetic storm. The molecule enriched air is carried to the middle latitudes by thermospheric neutral wind, and at the same time it diffuses away. When the molecule enriched air arrives at the F2 layer above a station, the electron loss rate in the F2 layer increases, the electron density decreases and then the negative phase at the station begins. We have calculated the variations of the fo F2 following magnetic storms for Manzhouli (29.5°N, 117.5°E), Freiburg (48°N, 07°E) and Billerica (43°N, 71°W) respectively. The results agree very well with typical events observed at the three stations and can be used to explain some average features of negative phase ionospheric storms in middle latitudes.
文摘A partially varying-coefficient model is one of the useful modelling tools.In this model, some coefficients of a linear model are kept to be constant whilst the others areallowed to vary with another factor. However, rarely can the analysts know a priori whichcoefficients can be assumed to be constant and which ones are varying with the given factor.Therefore, the identification problem of the constant coefficients should be solved before thepartially varying-coefficient model is used to analyze a real-world data set. In this article, asimple test method is proposed to achieve this task, in which the test statistic is constructed asthe sample variance of the estimates of each coefficient function in a well-knownvarying-coefficient model. Moreover two procedures, called F-approximation and three-moment χ~2approximation, are employed to derive the p-value of the test. Furthermore, some simulations areconducted to examine the performance of the test and the results are satisfactory.
文摘蚀变分带和成矿机制的准确厘定是建立斑岩成矿模型与找矿预测的关键。本文以新生代金沙江-哀牢山成矿带的玉龙斑岩铜矿为例,通过质量作用定律(LMA)和吉布斯自由能最小化模型(GEM),构建含矿热液与斑岩侵入体的pH-f O 2相图和动态传输模型,以揭示蚀变分带成因和金属成矿机制。LMA与GEM结果显示初始成矿流体pH值为4.7,logf_(O2)=-23.0(ΔFMQ=+2.7),且溶解Cu含量为1138×10^(-6),Mo为1.2×10^(-6)。研究表明,当该酸性及强氧化性流体流入二长花岗斑岩体时,在温度为450~360℃范围内,代表钾硅酸盐化蚀变的钾长石、黑云母、硬石膏、赤铁矿和磁铁矿的矿物逐渐沉淀,且与钾硅酸盐化蚀变相关流体具有较高pH值(5.0~7.0)和氧逸度(ΔFMQ=+2.9~+3.6)特征;当温度在360~320℃范围时,代表青磐岩化蚀变阶段的典型矿物如绿帘石、铁绿泥石和斜绿泥石等逐渐形成,流体pH值(5.0~6.4)和氧逸度(ΔFMQ=+1.1)均有所下降;当温度进一步从320℃下降到200℃时,流体pH值(5.0~5.7)进一步小幅下降,而氧逸度则(ΔFMQ=+1.7)略有回升,在此期间,绢云母和方解石等开始沉淀并形成典型的绢英岩化蚀变。此外,以HMoO_(4)^(-)和MoO_(4)^(2-)为载体的Mo在狭窄高温区间(450~370℃)内沉淀,而以CuCl(CuCl_(4)^(3-)、CuCl_(2)^(-)、CuCl)为主要载体的Cu则在在中、高温(450~300℃)范围中沉淀。通过利用LMA反演及GEM正演相结合定量化地刻画了玉龙斑岩铜矿水岩反应过程,由此揭示了斑岩矿床蚀变分带是逐渐冷却的单一岩浆热液与斑岩体不断反应的结果,且不同温度窗口对应着钾硅酸盐化(450~360℃)、青磐岩化(360~320℃)和绢英岩化(320~200℃)蚀变矿物的形成,故含矿流体温度的快速下降可能是玉龙铜矿蚀变叠加的重要因素。此外,Cu、Mo络合离子溶解度对温度变化的差异响应,导致了Mo矿化主要发育于靠近斑岩体的高温区域,而Cu则以网脉状-浸染状叠加到Mo矿化之上,并广泛分布于斑岩体周边的高-中温区域。