摘要
BACKGROUND： Pharmacological experiments have demonstrated that gastrodin has a protective effect on neonatal rat brain subjected to hypoxia-ischemia; however, the underlying mechanism has not been fully elucidated. OBJECTIVE： The aim of this study was to investigate the acute therapeutic effects of gastrodin by observing prostaglandin B2 and 6-keto-prostaglandin F 1 a in brain tissue of neonatal rats that received gastrodin injections immediately after hypoxia-ischemia. DESIGN： Single-factor design. SETTING： Department of Pediatrics, Affiliated Hospital of Yanbian University. MATERIALS： This study was performed in the Laboratory of the Department of Pediatrics, Affiliated Hospital of Yanbian University （key laboratory of provincial Health Department） from April to December 2003. Fifty-five Wistar rats of either gender, aged 7 days, were provided by the Laboratory Animal Center of Affiliated Hospital of Yanbian University. The rats were randomly divided into normal control （n =10）, model （n = 15）, gastrodin-treated （n = 15）, and Danshen-treated （n = 15） groups. The protocol was performed in accordance with guidelines from the Institute of Health Sciences for the use and care of animals. The following reagents were. used： Gastrodin （Sancai Medicine Group Co., Ltd., Zhongshan, Guangdong Province, China; component： gastrodin）, Danshen （Conba Stock Company, Jinhua, Zhengjiang Province, China; component： salvia miltiorrhiza）, and reagent kits for ^125I-prostaglandin B2 and ^125I-6-prostaglandin F l a （Research and Development Center for Science and Technology, General Hospital of Chinese PLA）. METHODS： Rats in the normal control group received no treatment. Rats in the remaining 3 groups were anesthetized, followed by ligation of the left common carotid artery. One hour later, the rats were placed in a closed hypoxic box and allowed to inhale 8% oxygen-air （2.0 3.0 L/min） for 2 hours to develop hypoxic-ischemic encephalopathy. Immediately after lesion, rats in the gastrodin and Danshen-treated groups were intraperitoneally injected with l g gastrodin （10 mL/kg） and 15 g Danshen （l 5 mL/kg）, respectively. MAIN OUTCOME MEASURES： Forty-eight hours after lesion, the left brain hemisphere was removed and homogenized to test the levels of prostaglandin B2 and 6-keto-prostaglandin F 1 a by radioimmunoassay. RESULTS： Forty successfully lesioned neonatal rats from the model, gastrodin-treated, and Danshen-treated groups, and ten rats from the control group, were included in the final analysis. Levels of prostaglandin B2 and 6-keto-prostaglandin F 1 a in brain tissue of neonatal rats were significantly higher in the model group than in the control group （both P 〈 0.01）. Levels of prostaglandin B2 and 6-keto-prostaglandin F 1 a were significantly lower in the gastrodin-treated and the Danshen-treated groups compared to the model group （all P 〈 0.01）. However, there were no significant differences in levels of prostaglandin B2 and 6-keto-prostaglandin F 1 a between the gastrodin-treated and the Danshen-treated groups （P 〉 0.05）. CONCLUSION： Gastrodin decreased prostaglandin and thromboxan levels in neonatal rat brains subjected to hypoxia-ischemia.
BACKGROUND： Pharmacological experiments have demonstrated that gastrodin has a protective effect on neonatal rat brain subjected to hypoxia-ischemia; however, the underlying mechanism has not been fully elucidated. OBJECTIVE： The aim of this study was to investigate the acute therapeutic effects of gastrodin by observing prostaglandin B2 and 6-keto-prostaglandin F 1 a in brain tissue of neonatal rats that received gastrodin injections immediately after hypoxia-ischemia. DESIGN： Single-factor design. SETTING： Department of Pediatrics, Affiliated Hospital of Yanbian University. MATERIALS： This study was performed in the Laboratory of the Department of Pediatrics, Affiliated Hospital of Yanbian University （key laboratory of provincial Health Department） from April to December 2003. Fifty-five Wistar rats of either gender, aged 7 days, were provided by the Laboratory Animal Center of Affiliated Hospital of Yanbian University. The rats were randomly divided into normal control （n =10）, model （n = 15）, gastrodin-treated （n = 15）, and Danshen-treated （n = 15） groups. The protocol was performed in accordance with guidelines from the Institute of Health Sciences for the use and care of animals. The following reagents were. used： Gastrodin （Sancai Medicine Group Co., Ltd., Zhongshan, Guangdong Province, China; component： gastrodin）, Danshen （Conba Stock Company, Jinhua, Zhengjiang Province, China; component： salvia miltiorrhiza）, and reagent kits for ^125I-prostaglandin B2 and ^125I-6-prostaglandin F l a （Research and Development Center for Science and Technology, General Hospital of Chinese PLA）. METHODS： Rats in the normal control group received no treatment. Rats in the remaining 3 groups were anesthetized, followed by ligation of the left common carotid artery. One hour later, the rats were placed in a closed hypoxic box and allowed to inhale 8% oxygen-air （2.0 3.0 L/min） for 2 hours to develop hypoxic-ischemic encephalopathy. Immediately after lesion, rats in the gastrodin and Danshen-treated groups were intraperitoneally injected with l g gastrodin （10 mL/kg） and 15 g Danshen （l 5 mL/kg）, respectively. MAIN OUTCOME MEASURES： Forty-eight hours after lesion, the left brain hemisphere was removed and homogenized to test the levels of prostaglandin B2 and 6-keto-prostaglandin F 1 a by radioimmunoassay. RESULTS： Forty successfully lesioned neonatal rats from the model, gastrodin-treated, and Danshen-treated groups, and ten rats from the control group, were included in the final analysis. Levels of prostaglandin B2 and 6-keto-prostaglandin F 1 a in brain tissue of neonatal rats were significantly higher in the model group than in the control group （both P 〈 0.01）. Levels of prostaglandin B2 and 6-keto-prostaglandin F 1 a were significantly lower in the gastrodin-treated and the Danshen-treated groups compared to the model group （all P 〈 0.01）. However, there were no significant differences in levels of prostaglandin B2 and 6-keto-prostaglandin F 1 a between the gastrodin-treated and the Danshen-treated groups （P 〉 0.05）. CONCLUSION： Gastrodin decreased prostaglandin and thromboxan levels in neonatal rat brains subjected to hypoxia-ischemia.
