Huajuhong(Exocarpium Citri grandis,ECG)is a traditional Chinese herbal medicine and has been used for the treatment of respiratory diseases for hundreds of years.Recently,ECG has been listed in a traditional Chinese m...Huajuhong(Exocarpium Citri grandis,ECG)is a traditional Chinese herbal medicine and has been used for the treatment of respiratory diseases for hundreds of years.Recently,ECG has been listed in a traditional Chinese medicine formula in the Guidelines for the Diagnosis and Treatment of Coronavirus Disease 2019(sixth edition)in China.To date,the effect and mechanism of ECG against respiratory diseases have not been systematically reviewed.In this paper,the researchers summarized the effects of ECG and its pharmacologically active compound naringin in functioning as an antitussive and expectorant,improving lung function,alleviating acute lung injury,attenuating pulmonary fibrosis,and enhancing antiviral immune response,so as to provide a reference for its clinical application in the prevention and treatment of multiple respiratory diseases,including coronavirus disease 2019.展开更多
Benincasae Exocarpium(BE,Dongguapi in Chinese),as the dried outer pericarp of Benincasa hispida(wax gourd)in Cucurbitaceae family,is one of traditional Chinese medicines with the same origin as medicine and food.Up to...Benincasae Exocarpium(BE,Dongguapi in Chinese),as the dried outer pericarp of Benincasa hispida(wax gourd)in Cucurbitaceae family,is one of traditional Chinese medicines with the same origin as medicine and food.Up to now,43 compounds were isolated from BE,including flavonoids,alkaloids,tannins,phenolic acids,soluble fiber and carbohydrates.Modern pharmacological studies and clinical practice showed that BE has diuretic,hypolipidemic effects,hypoglycemic,antioxidant,antibacterial,and other effects.The folk uses,functional factors,pharmacological activities,patents and clinical applications of BE were reviewed in this paper.In addition,the paper also discussed the current problems for the further studies.The information summarized in this paper provides valuable clues for the comprehensive utilization of medicine and food resources and gives a scientific basis for the development of medicinal plants of BE.展开更多
Objective:"Same treatment for different diseases"is a unique treatment strategy in traditional Chinese medicine.Two kinds of malignant respiratory diseases endanger human health-chronic obstructive pulmonary...Objective:"Same treatment for different diseases"is a unique treatment strategy in traditional Chinese medicine.Two kinds of malignant respiratory diseases endanger human health-chronic obstructive pulmonary disease(COPD)and lung cancer.Citrus Grandis Exocarpium(Huajuhong in Chinese,HJH),a famous herbal,is always applied by Chinese medicine practitioners to dispersion the lung to resolve phlegm based on"syndrome differentiation and treatment"theory.However,the common mechanism for HJH’s treatment of COPD and lung cancer is not clear.Methods:In this study,based on network pharmacology and molecular docking technology,the common mechanism of HJH in the treatment of COPD and lung cancer was studied.The active ingredients and related targets of HJH were integrated from TCMSP,BATMAN-TAM,STP,and Pubchem databases.The standard names of these targets were united by Uni Prot database.Targets of COPD and lung cancer were enriched through Gene Cards,NCBI(Gene),Therapeutic Target Database,and Dis Ge NET(v7.0)databases.Then the intersection targets of HJH and diseases were obtained.The STRING network and the Cytoscape 3.7.2 were used to construct PPI network,the DAVID database was used to perform GO and KEGG analysis.Then Cytoscape 3.6.1 was used to build"ingredient-target-signal pathway"network.Finally,Auto Dock 1.5.6 software was used to perform molecular docking of key proteins and molecules.Results:Eleven active ingredients in HJH were obtained by searching the database,corresponding to 184 HJH-COPD-lung cancer targets intersection.The results of biological network analysis showed that naringenin,the active component in HJH,could mainly act on target proteins such as AKT1,EGFR.Then through positive regulation of vasoconstriction and other biological processes,naringenin could regulate estrogen signaling pathway,VEGF signaling pathway,HIF-1 signaling pathway,Erb B signaling pathway,PI3K-Akt signaling pathway to play an important role in the treatment of both COPD and lung cancer.Conclusion:Network pharmacology was employed to systematically investigate the active ingredients and targets of HJH in treatment of COPD and lung cancer.And then,the common pharmacodynamic network of HJH for the two malignant respiratory diseases was firstly described.Furthermore,naringenin was proved to strongly bind with AKT1 and EGFR.It may provide the scientific basis for understanding the"Same treatment for different diseases"strategy in traditional Chinese medicine and inspirit subsequent drug discovery for COPD,lung cancer and other malignant lung diseases.展开更多
目的基于色差计技术,建立蜜化橘红炮制程度判别函数,并探究蜜化橘红外观颜色与内在成分的关联性。方法使用色差计获取80份蜜化橘红样品的智能感官信息,利用专家评分、偏最小二乘法判别(partial least squares discriminant analysis,PLS...目的基于色差计技术,建立蜜化橘红炮制程度判别函数,并探究蜜化橘红外观颜色与内在成分的关联性。方法使用色差计获取80份蜜化橘红样品的智能感官信息,利用专家评分、偏最小二乘法判别(partial least squares discriminant analysis,PLS-DA)、Fisher线性判别对80份样品进行分析并建立蜜化橘红炮制程度的判别函数;使用色差计对蜜化橘红炒制过程中的样品进行色度值测定,结合HPLC指纹图谱分析蜜化橘红炒制过程中样品共有成分相对含量的变化,同时利用多元统计学分析方法对蜜化橘红炒制过程中的样品进行颜色与成分的关联性分析。结果建立的判别函数为Y_(1)=2.197ΔL^(*)−0.150Δa^(*)−0.484Δb^(*)+1.444ΔE^(*)ab+3.064;Y_(2)=0.479ΔL^(*)−0.319Δa^(*)−0.795Δb^(*)+0.433ΔE^(*)ab+0.371,判别正确率为95%~100%。外观颜色与内在成分斯皮尔曼分析结果显示,与颜色参数值显著相关的内在成分主要为峰1~4、峰9~11、峰15~16;共有成分PLS-DA分析结果显示,得到VIP>1的分别为峰11、5、10、15、7、13,其中峰3和11分别为5-HMF、佛手酚。结论基于智能感官技术结合化学计量学建立的蜜化橘红炮制程度判别函数可用于大部分蜜化橘红样判别,预测准确度较高,为蜜化橘红炮制程度快速辨识提供参考;内在成分与外观颜色的动态关联研究中,筛选出既与颜色变化有显著相关性,又在蜜化橘红炒制过程中含量变化较大的峰11、10、15。其中峰11为佛手酚,推荐可将其作为蜜化橘红饮片质量控制候选标志物。展开更多
基金Science and Technology Planning Project of Guangdong Province in China(2018A030313491,2019B090905002).
文摘Huajuhong(Exocarpium Citri grandis,ECG)is a traditional Chinese herbal medicine and has been used for the treatment of respiratory diseases for hundreds of years.Recently,ECG has been listed in a traditional Chinese medicine formula in the Guidelines for the Diagnosis and Treatment of Coronavirus Disease 2019(sixth edition)in China.To date,the effect and mechanism of ECG against respiratory diseases have not been systematically reviewed.In this paper,the researchers summarized the effects of ECG and its pharmacologically active compound naringin in functioning as an antitussive and expectorant,improving lung function,alleviating acute lung injury,attenuating pulmonary fibrosis,and enhancing antiviral immune response,so as to provide a reference for its clinical application in the prevention and treatment of multiple respiratory diseases,including coronavirus disease 2019.
基金supported by the National Natural Science Foundation of China(No.81673693)Tianjin Intelligent Manufacturing Special Project of China(No.20201178)for financial support.
文摘Benincasae Exocarpium(BE,Dongguapi in Chinese),as the dried outer pericarp of Benincasa hispida(wax gourd)in Cucurbitaceae family,is one of traditional Chinese medicines with the same origin as medicine and food.Up to now,43 compounds were isolated from BE,including flavonoids,alkaloids,tannins,phenolic acids,soluble fiber and carbohydrates.Modern pharmacological studies and clinical practice showed that BE has diuretic,hypolipidemic effects,hypoglycemic,antioxidant,antibacterial,and other effects.The folk uses,functional factors,pharmacological activities,patents and clinical applications of BE were reviewed in this paper.In addition,the paper also discussed the current problems for the further studies.The information summarized in this paper provides valuable clues for the comprehensive utilization of medicine and food resources and gives a scientific basis for the development of medicinal plants of BE.
基金financially supported by the National Key Research and Development Program of China (2018YFC1706300 and 2018YFC17063005)the Science and Technology Planning Project of Gansu Province (20JR10RA586)+2 种基金the Fundamental Research Funds for the Central Universities (lzujbky-2021-kb40)the Project for Longyuan Youth Innovation and Entrepreneurship Talentthe Science and Technology Planning Project of Guangdong Province of China (2019B090905002)
文摘Objective:"Same treatment for different diseases"is a unique treatment strategy in traditional Chinese medicine.Two kinds of malignant respiratory diseases endanger human health-chronic obstructive pulmonary disease(COPD)and lung cancer.Citrus Grandis Exocarpium(Huajuhong in Chinese,HJH),a famous herbal,is always applied by Chinese medicine practitioners to dispersion the lung to resolve phlegm based on"syndrome differentiation and treatment"theory.However,the common mechanism for HJH’s treatment of COPD and lung cancer is not clear.Methods:In this study,based on network pharmacology and molecular docking technology,the common mechanism of HJH in the treatment of COPD and lung cancer was studied.The active ingredients and related targets of HJH were integrated from TCMSP,BATMAN-TAM,STP,and Pubchem databases.The standard names of these targets were united by Uni Prot database.Targets of COPD and lung cancer were enriched through Gene Cards,NCBI(Gene),Therapeutic Target Database,and Dis Ge NET(v7.0)databases.Then the intersection targets of HJH and diseases were obtained.The STRING network and the Cytoscape 3.7.2 were used to construct PPI network,the DAVID database was used to perform GO and KEGG analysis.Then Cytoscape 3.6.1 was used to build"ingredient-target-signal pathway"network.Finally,Auto Dock 1.5.6 software was used to perform molecular docking of key proteins and molecules.Results:Eleven active ingredients in HJH were obtained by searching the database,corresponding to 184 HJH-COPD-lung cancer targets intersection.The results of biological network analysis showed that naringenin,the active component in HJH,could mainly act on target proteins such as AKT1,EGFR.Then through positive regulation of vasoconstriction and other biological processes,naringenin could regulate estrogen signaling pathway,VEGF signaling pathway,HIF-1 signaling pathway,Erb B signaling pathway,PI3K-Akt signaling pathway to play an important role in the treatment of both COPD and lung cancer.Conclusion:Network pharmacology was employed to systematically investigate the active ingredients and targets of HJH in treatment of COPD and lung cancer.And then,the common pharmacodynamic network of HJH for the two malignant respiratory diseases was firstly described.Furthermore,naringenin was proved to strongly bind with AKT1 and EGFR.It may provide the scientific basis for understanding the"Same treatment for different diseases"strategy in traditional Chinese medicine and inspirit subsequent drug discovery for COPD,lung cancer and other malignant lung diseases.
文摘目的基于色差计技术,建立蜜化橘红炮制程度判别函数,并探究蜜化橘红外观颜色与内在成分的关联性。方法使用色差计获取80份蜜化橘红样品的智能感官信息,利用专家评分、偏最小二乘法判别(partial least squares discriminant analysis,PLS-DA)、Fisher线性判别对80份样品进行分析并建立蜜化橘红炮制程度的判别函数;使用色差计对蜜化橘红炒制过程中的样品进行色度值测定,结合HPLC指纹图谱分析蜜化橘红炒制过程中样品共有成分相对含量的变化,同时利用多元统计学分析方法对蜜化橘红炒制过程中的样品进行颜色与成分的关联性分析。结果建立的判别函数为Y_(1)=2.197ΔL^(*)−0.150Δa^(*)−0.484Δb^(*)+1.444ΔE^(*)ab+3.064;Y_(2)=0.479ΔL^(*)−0.319Δa^(*)−0.795Δb^(*)+0.433ΔE^(*)ab+0.371,判别正确率为95%~100%。外观颜色与内在成分斯皮尔曼分析结果显示,与颜色参数值显著相关的内在成分主要为峰1~4、峰9~11、峰15~16;共有成分PLS-DA分析结果显示,得到VIP>1的分别为峰11、5、10、15、7、13,其中峰3和11分别为5-HMF、佛手酚。结论基于智能感官技术结合化学计量学建立的蜜化橘红炮制程度判别函数可用于大部分蜜化橘红样判别,预测准确度较高,为蜜化橘红炮制程度快速辨识提供参考;内在成分与外观颜色的动态关联研究中,筛选出既与颜色变化有显著相关性,又在蜜化橘红炒制过程中含量变化较大的峰11、10、15。其中峰11为佛手酚,推荐可将其作为蜜化橘红饮片质量控制候选标志物。
