Introduction: Ewings sarcoma is uncommon in extra osseous locations. They belong to Ewings family of tumours and the primitive neuroectodermal tumour is often diagnosed based on immuno histochemistry and molecular gen...Introduction: Ewings sarcoma is uncommon in extra osseous locations. They belong to Ewings family of tumours and the primitive neuroectodermal tumour is often diagnosed based on immuno histochemistry and molecular genetic studies. Discussion: A 50-year-old gentleman presented with complaints of a swelling in the right thigh. With a diagnosis of soft tissue sarcoma, the patient underwent a wide local excision of the tumour. We review literature of the small round blue cell tumour. Conclusion: Extraosseous Ewings sarcoma presenting as a thigh swelling is uncommon. The sarcoma was vimentin positive and CD99 membrane positivity.展开更多
We report a case of Ewings sarcoma(ES) involving the patella in a young female. ES of patella is a rare entity. The patient was presented with anterior knee pain and swelling arising from the patella. She was treated ...We report a case of Ewings sarcoma(ES) involving the patella in a young female. ES of patella is a rare entity. The patient was presented with anterior knee pain and swelling arising from the patella. She was treated with neoadjuvant chemotherapy followed by wide excision of the patella and reconstruction of the extensor mechanism using split tendoachilles auto graft. The patella is an uncommon site for primary or metastatic tumors of the bone. ES, though rare, should be included in the differential diagnosis of swellings arising from the patella. Auto graft from the tendoachilles is a good alternative for reconstructing the extensor mechanism of the knee.展开更多
Background:Photodynamic therapy(PDT)may eradicate residual malignant cells following sarcoma resection,through reactive oxygen species(ROS)mediated cytotoxicity,thus improve clinical outcomes.This study aims to assess...Background:Photodynamic therapy(PDT)may eradicate residual malignant cells following sarcoma resection,through reactive oxygen species(ROS)mediated cytotoxicity,thus improve clinical outcomes.This study aims to assess the efficacy of 5-aminolevulinic acid(5-ALA)as a photosensitizer in combination with red light(RL)for PDT of bone sarcoma cells in vitro.Methods:Three bone sarcoma cell lines underwent treatment with 5-ALA and RL or sham-RL(SL).5-ALA uptake was assessed using flow cytometry.Production of ROS was measured using CellROX Green staining and fluorescence microscopy.Cell viability was assessed using Cell Counting Kit-8 assays.Results:All cell lines showed significant 5-ALA uptake in comparison to the 0 mM control(p<0.05).Production of ROS was significantly increased in cells treated with 5-ALA and RL,compared to those treated with RL and no 5-ALA or SL(p<0.05).Viability was significantly reduced in cells treated with 5-ALA and RL,compared to SL(p<0.05).At 72 h post-treatment,cell viability ranged from 6%-12%in 0.5 mM 5-ALA and RL-treated cells vs.90%-137%in 0.5 mM 5-ALA and SL-treated cells.Conclusion:5-ALA-based PDT led to the desired increased production of ROS and reduction in cell viability in all cell lines.These preliminary in vitro results warrant further study with multicellular spheroid or animal models and suggest PDT has potential to be used as an adjuvant therapy to surgical resection in sarcoma management.展开更多
Osteosarcoma(OS),chondrosarcoma(CS),and Ewing sarcoma(ES)represent primary malignant bone tumors and pose significant challenges in oncology research and clinical management.Conventional research methods,such as two-d...Osteosarcoma(OS),chondrosarcoma(CS),and Ewing sarcoma(ES)represent primary malignant bone tumors and pose significant challenges in oncology research and clinical management.Conventional research methods,such as two-dimensional(2D)cultured tumor cells and animal models,have limitations in recapitulating the complex tumor microenvironment(TME)and often fail to translate into effective clinical treatments.The advancement of three-dimensional(3D)culture technology has revolutionized the field by enabling the development of in vitro constructed bone tumor models that closely mimic the in vivo TME.These models provide powerful tools for investigating tumor biology,assessing therapeutic responses,and advancing personalized medicine.This comprehensive review summarizes the recent advancements in research on 3D tumor models constructed in vitro for OS,CS,and ES.We discuss the various techniques employed in model construction,their applications,and the challenges and future directions in this field.The integration of advanced technologies and the incorporation of additional cell types hold promise for the development of more sophisticated and physiologically relevant models.As research in this field continues to evolve,we anticipate that these models will play an increasingly crucial role in unraveling the complexities of malignant bone tumors and accelerating the development of novel therapeutic strategies.展开更多
Adamantinoma-like Ewing sarcoma(ALES)is a rare variant of Ewing sarcoma that predominantly occurs in the head and neck region,characterized by epithelial differentiation and overlapping morphology with other small rou...Adamantinoma-like Ewing sarcoma(ALES)is a rare variant of Ewing sarcoma that predominantly occurs in the head and neck region,characterized by epithelial differentiation and overlapping morphology with other small round cell tumors.We present three cases of head and neck ALES(two in the parotid gland and one in the nasal cavity)in patients aged 23-42 years.Histologically,the tumors exhibited a spectrum of features,including small round blue cells and basaloid squamous morphologies.Immunohistochemically,the tumor cells variably expressed CKpan,p63,CD99,NKX2.2,FLI1,CD56,and Syn.The Ki-67 proliferation index ranged widely from 15%to 90%.Notably,one case initially misdiagnosed as squamous cell carcinoma harbored a canonical EWSR1::FLI1 fusion.Crucially,we report the first case of ALES had a negative EWSR1 rearrangement but a somatic NF1 mutation,expanding the molecular spectrum of this entity.Our findings underscore the diagnostic challenge of ALES and highlight the necessity of comprehensive molecular profiling,including next-generation sequencing,for accurate diagnosis and the identification of potential therapeutic targets,particularly in genetically atypical cases.展开更多
Real-time assessment of slope reinforcements to diagnose their state in all stages of service life is imperative for prompt evaluation of slope stability and establishing an efficient early warning(EW)system.Many poin...Real-time assessment of slope reinforcements to diagnose their state in all stages of service life is imperative for prompt evaluation of slope stability and establishing an efficient early warning(EW)system.Many point-based monitoring instruments have been used in the last few decades.However,these sensors suffer from a particular risk of detection failures and practical limitations.Fibre-optic sensing(FOS)technologies have been developed,tested,and validated across various geoengineering applications,including slope monitoring,as they offer exceptional advantages,such as high data-carrying capacity,precise mapping of physical parameters,durability,and immunity to electromagnetic interference.The deformation of rock/soil causes the deformation and fracture of reinforcement materials,which are subsequently transferred to the encapsulated fibre-optic(FO)sensors,providing valuable information on reinforcements'safety state and performance for early failure detection.This paper is devoted to critically analysing the application of cutting-edge FOS technologies for slope reinforcement monitoring.Firstly,a concise overview of the fundamental principles underlying discrete and distributed FOS methods is provided.The key considerations for selecting FO cables and the appropriate packaging techniques necessary to withstand the challenges posed by complex geological environments are also summarised.We delve into the details of three distinct cable installation techniques within slope reinforcement components:surface bonding,slot embedment,and clamping.The recent advancements in FOS methods for monitoring slope reinforcements such as rock bolts,soil nails,anti-slide piles,geosynthetics,and retaining walls are extensively reviewed.The paper addresses this novel sensing technique's challenges and comprehensively explores its prospects.This review is anticipated to be a valuable resource for geoengineers and researchers involved in slope monitoring through FOS technology,offering insightful perspectives and guidance.展开更多
Floods and storm surges pose significant threats to coastal regions worldwide,demanding timely and accurate early warning systems(EWS)for disaster preparedness.Traditional numerical and statistical methods often fall ...Floods and storm surges pose significant threats to coastal regions worldwide,demanding timely and accurate early warning systems(EWS)for disaster preparedness.Traditional numerical and statistical methods often fall short in capturing complex,nonlinear,and real-time environmental dynamics.In recent years,machine learning(ML)and deep learning(DL)techniques have emerged as promising alternatives for enhancing the accuracy,speed,and scalability of EWS.This review critically evaluates the evolution of ML models—such as Artificial Neural Networks(ANN),Convolutional Neural Networks(CNN),and Long Short-Term Memory(LSTM)—in coastal flood prediction,highlighting their architectures,data requirements,performance metrics,and implementation challenges.A unique contribution of this work is the synthesis of real-time deployment challenges including latency,edge-cloud tradeoffs,and policy-level integration,areas often overlooked in prior literature.Furthermore,the review presents a comparative framework of model performance across different geographic and hydrologic settings,offering actionable insights for researchers and practitioners.Limitations of current AI-driven models,such as interpretability,data scarcity,and generalization across regions,are discussed in detail.Finally,the paper outlines future research directions including hybrid modelling,transfer learning,explainable AI,and policy-aware alert systems.By bridging technical performance and operational feasibility,this review aims to guide the development of next-generation intelligent EWS for resilient and adaptive coastal management.展开更多
文摘Introduction: Ewings sarcoma is uncommon in extra osseous locations. They belong to Ewings family of tumours and the primitive neuroectodermal tumour is often diagnosed based on immuno histochemistry and molecular genetic studies. Discussion: A 50-year-old gentleman presented with complaints of a swelling in the right thigh. With a diagnosis of soft tissue sarcoma, the patient underwent a wide local excision of the tumour. We review literature of the small round blue cell tumour. Conclusion: Extraosseous Ewings sarcoma presenting as a thigh swelling is uncommon. The sarcoma was vimentin positive and CD99 membrane positivity.
文摘We report a case of Ewings sarcoma(ES) involving the patella in a young female. ES of patella is a rare entity. The patient was presented with anterior knee pain and swelling arising from the patella. She was treated with neoadjuvant chemotherapy followed by wide excision of the patella and reconstruction of the extensor mechanism using split tendoachilles auto graft. The patella is an uncommon site for primary or metastatic tumors of the bone. ES, though rare, should be included in the differential diagnosis of swellings arising from the patella. Auto graft from the tendoachilles is a good alternative for reconstructing the extensor mechanism of the knee.
文摘Background:Photodynamic therapy(PDT)may eradicate residual malignant cells following sarcoma resection,through reactive oxygen species(ROS)mediated cytotoxicity,thus improve clinical outcomes.This study aims to assess the efficacy of 5-aminolevulinic acid(5-ALA)as a photosensitizer in combination with red light(RL)for PDT of bone sarcoma cells in vitro.Methods:Three bone sarcoma cell lines underwent treatment with 5-ALA and RL or sham-RL(SL).5-ALA uptake was assessed using flow cytometry.Production of ROS was measured using CellROX Green staining and fluorescence microscopy.Cell viability was assessed using Cell Counting Kit-8 assays.Results:All cell lines showed significant 5-ALA uptake in comparison to the 0 mM control(p<0.05).Production of ROS was significantly increased in cells treated with 5-ALA and RL,compared to those treated with RL and no 5-ALA or SL(p<0.05).Viability was significantly reduced in cells treated with 5-ALA and RL,compared to SL(p<0.05).At 72 h post-treatment,cell viability ranged from 6%-12%in 0.5 mM 5-ALA and RL-treated cells vs.90%-137%in 0.5 mM 5-ALA and SL-treated cells.Conclusion:5-ALA-based PDT led to the desired increased production of ROS and reduction in cell viability in all cell lines.These preliminary in vitro results warrant further study with multicellular spheroid or animal models and suggest PDT has potential to be used as an adjuvant therapy to surgical resection in sarcoma management.
基金supported by the Zhejiang Province Basic Public Welfare Research Program(No.TGD24H160005),China。
文摘Osteosarcoma(OS),chondrosarcoma(CS),and Ewing sarcoma(ES)represent primary malignant bone tumors and pose significant challenges in oncology research and clinical management.Conventional research methods,such as two-dimensional(2D)cultured tumor cells and animal models,have limitations in recapitulating the complex tumor microenvironment(TME)and often fail to translate into effective clinical treatments.The advancement of three-dimensional(3D)culture technology has revolutionized the field by enabling the development of in vitro constructed bone tumor models that closely mimic the in vivo TME.These models provide powerful tools for investigating tumor biology,assessing therapeutic responses,and advancing personalized medicine.This comprehensive review summarizes the recent advancements in research on 3D tumor models constructed in vitro for OS,CS,and ES.We discuss the various techniques employed in model construction,their applications,and the challenges and future directions in this field.The integration of advanced technologies and the incorporation of additional cell types hold promise for the development of more sophisticated and physiologically relevant models.As research in this field continues to evolve,we anticipate that these models will play an increasingly crucial role in unraveling the complexities of malignant bone tumors and accelerating the development of novel therapeutic strategies.
文摘Adamantinoma-like Ewing sarcoma(ALES)is a rare variant of Ewing sarcoma that predominantly occurs in the head and neck region,characterized by epithelial differentiation and overlapping morphology with other small round cell tumors.We present three cases of head and neck ALES(two in the parotid gland and one in the nasal cavity)in patients aged 23-42 years.Histologically,the tumors exhibited a spectrum of features,including small round blue cells and basaloid squamous morphologies.Immunohistochemically,the tumor cells variably expressed CKpan,p63,CD99,NKX2.2,FLI1,CD56,and Syn.The Ki-67 proliferation index ranged widely from 15%to 90%.Notably,one case initially misdiagnosed as squamous cell carcinoma harbored a canonical EWSR1::FLI1 fusion.Crucially,we report the first case of ALES had a negative EWSR1 rearrangement but a somatic NF1 mutation,expanding the molecular spectrum of this entity.Our findings underscore the diagnostic challenge of ALES and highlight the necessity of comprehensive molecular profiling,including next-generation sequencing,for accurate diagnosis and the identification of potential therapeutic targets,particularly in genetically atypical cases.
基金funding support from JSPS KAKENHI(Grant Nos.21H01593 and 21K18794)through Tetsuya KogureThis work was also partially supported by the Sasakawa Scientific Research Grant(2023e2026)from the Japan Science Society(JSS)through Ashis Acharya.
文摘Real-time assessment of slope reinforcements to diagnose their state in all stages of service life is imperative for prompt evaluation of slope stability and establishing an efficient early warning(EW)system.Many point-based monitoring instruments have been used in the last few decades.However,these sensors suffer from a particular risk of detection failures and practical limitations.Fibre-optic sensing(FOS)technologies have been developed,tested,and validated across various geoengineering applications,including slope monitoring,as they offer exceptional advantages,such as high data-carrying capacity,precise mapping of physical parameters,durability,and immunity to electromagnetic interference.The deformation of rock/soil causes the deformation and fracture of reinforcement materials,which are subsequently transferred to the encapsulated fibre-optic(FO)sensors,providing valuable information on reinforcements'safety state and performance for early failure detection.This paper is devoted to critically analysing the application of cutting-edge FOS technologies for slope reinforcement monitoring.Firstly,a concise overview of the fundamental principles underlying discrete and distributed FOS methods is provided.The key considerations for selecting FO cables and the appropriate packaging techniques necessary to withstand the challenges posed by complex geological environments are also summarised.We delve into the details of three distinct cable installation techniques within slope reinforcement components:surface bonding,slot embedment,and clamping.The recent advancements in FOS methods for monitoring slope reinforcements such as rock bolts,soil nails,anti-slide piles,geosynthetics,and retaining walls are extensively reviewed.The paper addresses this novel sensing technique's challenges and comprehensively explores its prospects.This review is anticipated to be a valuable resource for geoengineers and researchers involved in slope monitoring through FOS technology,offering insightful perspectives and guidance.
文摘Floods and storm surges pose significant threats to coastal regions worldwide,demanding timely and accurate early warning systems(EWS)for disaster preparedness.Traditional numerical and statistical methods often fall short in capturing complex,nonlinear,and real-time environmental dynamics.In recent years,machine learning(ML)and deep learning(DL)techniques have emerged as promising alternatives for enhancing the accuracy,speed,and scalability of EWS.This review critically evaluates the evolution of ML models—such as Artificial Neural Networks(ANN),Convolutional Neural Networks(CNN),and Long Short-Term Memory(LSTM)—in coastal flood prediction,highlighting their architectures,data requirements,performance metrics,and implementation challenges.A unique contribution of this work is the synthesis of real-time deployment challenges including latency,edge-cloud tradeoffs,and policy-level integration,areas often overlooked in prior literature.Furthermore,the review presents a comparative framework of model performance across different geographic and hydrologic settings,offering actionable insights for researchers and practitioners.Limitations of current AI-driven models,such as interpretability,data scarcity,and generalization across regions,are discussed in detail.Finally,the paper outlines future research directions including hybrid modelling,transfer learning,explainable AI,and policy-aware alert systems.By bridging technical performance and operational feasibility,this review aims to guide the development of next-generation intelligent EWS for resilient and adaptive coastal management.
