Objective:To investigate the effect of Chinese herbal formula Ermiao Powder(二妙散,EMP)on the expression of cholinergic anti-inflammatory pathway in rats with rheumatoid arthritis(RA).Methods:Seventytwo rats were rand...Objective:To investigate the effect of Chinese herbal formula Ermiao Powder(二妙散,EMP)on the expression of cholinergic anti-inflammatory pathway in rats with rheumatoid arthritis(RA).Methods:Seventytwo rats were randomly divided into 6 groups according to body weight,including normal control group,collageninduced arthritis(CIA)group,three doses EMP groups,and methotrexate(MTX)group(n=12 per group).All of the rats except for those in the normal control group were given multipoint subcutaneous injection of bovine typeⅡcollagen to establish a CIA model.Three EMP groups received a high-(4.5 g/kg),medium-(3.0 g/kg),and low-(1.5 g/kg)doses of EMP by intragavage,respectively.MTX group was injected intraperitoneally MTX at 0.9 mg/kg once a week as the positive control.The administration was 3 consecutive weeks.Joint swelling,arthritis index,and body weight changes in different experimental groups of rats were tested.The joint damage was evaluated by masson staining.Quantitative real-time polymerase chain reaction,Western blot,and immunohistochemistry(IHC)were performed to evaluate the expression of CHRNA7,encodingα7 nicotinic acetylcholine receptor in the cholinergic anti-inflammatory pathway,in different tissues and their localization in the spleen and joints.Results:CHRNA7 expression levels were significantly higher in the joints and spleens of CIA group than those in normal control group(both P<0.05).Moreover,the CHRNA7 mRNA and protein levels in the spleen and joints of MTX and three doses of EMP groups were significantly lower than CIA group(all P<0.05).Compared with the MTX group,treatment with low-dose EMP resulted in significant reduction of CHRNA7 mRNA and protein expression levels(P<0.05 or P<0.01).IHC showed positive signals of CHRNA7 in the white pulp and red pulp of the spleens of rats;CHRNA7 was expressed on fibroblast-like synoviocytes,macrophages,and endothelial cells in the joints of rats,and the expression in the joints of low-dose EMP group was significantly lower than that in the CIA group(P<0.01).Conclusion:Cholinergic anti-inflammatory pathway was involved in the generation of the inflammatory reaction in CIA rats,and EMP exerted therapeutic effect on RA through cholinergic anti-inflammatory pathway.展开更多
The classified prescriptions from Chaihu decoction have been widely used in ancient and modern times.In spite of many previous research studies,the extensiveness and diversity of their applications have not been syste...The classified prescriptions from Chaihu decoction have been widely used in ancient and modern times.In spite of many previous research studies,the extensiveness and diversity of their applications have not been systematically studied.The author applied Chaihu Wendan decoction to treat phlegm and qi stagnation in the middle energizer,applied Chaihu Guizhi decoction to treat simultaneous disease of Shaoyang and Taiyang,applied Chaihu Xiexin decoction to treat simultaneous disease of the spleen,stomach,liver,and gallbladder,applied Chaihu Ermiao powder to treat simultaneous disease of hand Shaoyang and foot Taiyin,applied Chaihu Erchen decoction to treat simultaneous disease of Shaoyang and Taiyin,applied Chailing decoction to treat simultaneous disease of hand Shaoyin and foot Taiyang,applied Chaihu Guizhi Ganjiang decoction to treat Shaoyang and Taiyin cold fluid retention and heat stagnation syndrome of Shaoyang complicated with Taiyin,applied Chaihu Xingren decoction to treat simultaneous disease of hand Shaoyang and hand Taiyin,applied Chaihu Sanren decoction to treat the disease caused by damp-heat stagnation in the hand Shaoyang and the foot Taiyin,and applied Chaihu Ganlu Xiaodu pellet to treat the diseases of hand Shaoyang and foot Yangming.The effects were all remarkable.展开更多
基金Supported by the National Natural Science Foundation of China(No.81904034)the Basic Research Project of Shanxi Province(No.201801D121228)+1 种基金the Scientific and Technological Innovation Programs of Higher Education Institutions in Shanxi(No.201804001)the Research Project of Health Commission of Shanxi Province(No.2018001)。
文摘Objective:To investigate the effect of Chinese herbal formula Ermiao Powder(二妙散,EMP)on the expression of cholinergic anti-inflammatory pathway in rats with rheumatoid arthritis(RA).Methods:Seventytwo rats were randomly divided into 6 groups according to body weight,including normal control group,collageninduced arthritis(CIA)group,three doses EMP groups,and methotrexate(MTX)group(n=12 per group).All of the rats except for those in the normal control group were given multipoint subcutaneous injection of bovine typeⅡcollagen to establish a CIA model.Three EMP groups received a high-(4.5 g/kg),medium-(3.0 g/kg),and low-(1.5 g/kg)doses of EMP by intragavage,respectively.MTX group was injected intraperitoneally MTX at 0.9 mg/kg once a week as the positive control.The administration was 3 consecutive weeks.Joint swelling,arthritis index,and body weight changes in different experimental groups of rats were tested.The joint damage was evaluated by masson staining.Quantitative real-time polymerase chain reaction,Western blot,and immunohistochemistry(IHC)were performed to evaluate the expression of CHRNA7,encodingα7 nicotinic acetylcholine receptor in the cholinergic anti-inflammatory pathway,in different tissues and their localization in the spleen and joints.Results:CHRNA7 expression levels were significantly higher in the joints and spleens of CIA group than those in normal control group(both P<0.05).Moreover,the CHRNA7 mRNA and protein levels in the spleen and joints of MTX and three doses of EMP groups were significantly lower than CIA group(all P<0.05).Compared with the MTX group,treatment with low-dose EMP resulted in significant reduction of CHRNA7 mRNA and protein expression levels(P<0.05 or P<0.01).IHC showed positive signals of CHRNA7 in the white pulp and red pulp of the spleens of rats;CHRNA7 was expressed on fibroblast-like synoviocytes,macrophages,and endothelial cells in the joints of rats,and the expression in the joints of low-dose EMP group was significantly lower than that in the CIA group(P<0.01).Conclusion:Cholinergic anti-inflammatory pathway was involved in the generation of the inflammatory reaction in CIA rats,and EMP exerted therapeutic effect on RA through cholinergic anti-inflammatory pathway.
基金supported by Jiangxi Provincial Health Department Chinese Medicine Research Program Project{[2004]No.28}.
文摘The classified prescriptions from Chaihu decoction have been widely used in ancient and modern times.In spite of many previous research studies,the extensiveness and diversity of their applications have not been systematically studied.The author applied Chaihu Wendan decoction to treat phlegm and qi stagnation in the middle energizer,applied Chaihu Guizhi decoction to treat simultaneous disease of Shaoyang and Taiyang,applied Chaihu Xiexin decoction to treat simultaneous disease of the spleen,stomach,liver,and gallbladder,applied Chaihu Ermiao powder to treat simultaneous disease of hand Shaoyang and foot Taiyin,applied Chaihu Erchen decoction to treat simultaneous disease of Shaoyang and Taiyin,applied Chailing decoction to treat simultaneous disease of hand Shaoyin and foot Taiyang,applied Chaihu Guizhi Ganjiang decoction to treat Shaoyang and Taiyin cold fluid retention and heat stagnation syndrome of Shaoyang complicated with Taiyin,applied Chaihu Xingren decoction to treat simultaneous disease of hand Shaoyang and hand Taiyin,applied Chaihu Sanren decoction to treat the disease caused by damp-heat stagnation in the hand Shaoyang and the foot Taiyin,and applied Chaihu Ganlu Xiaodu pellet to treat the diseases of hand Shaoyang and foot Yangming.The effects were all remarkable.
