This work aimed to investigate the biosorption of lead by free and immobilized bacterial cell. Twenty four samples were collected from the wastewater of batteries factory in AL-Waziria district for the period from Jun...This work aimed to investigate the biosorption of lead by free and immobilized bacterial cell. Twenty four samples were collected from the wastewater of batteries factory in AL-Waziria district for the period from June to Sept. 2004, three species of bacteria have been isolated from this wastewater： Enterobacter agglomerans, E. gergoviae, and E. sakazakii. The results revealed a high variation in the ability of these bacteria for lead biosorption, the bacteria E. agglomerans （ES） was the most efficient compared to the other bacterial species. A laboratory plant with continuous flow system was designed for lead ions removal from standards aqueous solutions, as the cells were immobilized using： white sand, charcoal, agar and calcium alginate. However, the cells immobilized to calcium alginate were the most efficient in removal of lead ions （98%）. Present study also determined the optimal conditions for the biosorptJon of lead ions which were temperature 40℃ and pH 4 with one hour contiguity between the immobilized bacteria and solution. Different washing solutions were used fo~： recovering the lead ions that have been biosorbed on the bacterial walls, 0.1 M EDTA was the most efficient. Preferential biosorption by E. agglomerans （E5） to copper and cadmium was determined, result showed that the order of this preferential capability was： Cu 〉 Pb 〉 Cd. No significant differences were found in the biosorption of lead ions by alive and dead E. agglomerans （E5）. The designed lab plant containing bacterial cells binding on calcium alginate was able to uptake all lead ions from all samples of industrial wastewater of batteries factory.展开更多
Recently,Prevotella spp.,a major genus of gram-negative commensal bacteria in humans,have emerged as a key microbial contributor to host metabolism due to its ability to ferment dietary fibers,produce beneficial short...Recently,Prevotella spp.,a major genus of gram-negative commensal bacteria in humans,have emerged as a key microbial contributor to host metabolism due to its ability to ferment dietary fibers,produce beneficial short-chain fatty acids,and influence immune responses.However,their diversity and functional differences have created challenges for their development and therapeutic use.Recent studies have shown that specific Prevotella species,such as P.copri,P.intestinalis,and P.histicola,can strengthen gut barrier integrity and reduce metabolic imbalances.Notably,Prevotella populations can be increased through high-fiber or herbal-based treatments.Traditional herbal medicines,including fiber-rich decoctions,also demonstrate the potential to boost endogenous Prevotella communities,enhance microbial fermentation,and improve glucose and lipid balance.This perspective examines the context-dependent roles of Prevotella spp.,with emphasis on the functional heterogeneity of key species such as P.copri,suggests a framework for combining herbal modulation with species-level microbiota profiling,and outlines a research plan to explore microbe-herb synergy in treating obesity,type 2 diabetes,and related metabolic disorders.This strategy offers a new,ecology-based approach to complement standard metabolic interventions.展开更多
Objective:To investigate the quantitative assessment efficacy of chest CT combined with serum Vanin-1 and SPP1 in determining the progression stage of chronic obstructive pulmonary disease(COPD).Methods:A total of 100...Objective:To investigate the quantitative assessment efficacy of chest CT combined with serum Vanin-1 and SPP1 in determining the progression stage of chronic obstructive pulmonary disease(COPD).Methods:A total of 100 COPD subjects from our hospital from January 2020 to December 2023 were included and randomly divided into a healthy control group and an experimental group(50 cases each).The healthy control group underwent slow vital capacity measurement using a spirometer,while the experimental group underwent high-resolution thin-slice CT scans and serum Vanin-1 and SPP1 concentration measurements.Pulmonary function parameters,symptom burden,biomarker concentrations,and imaging characteristics were compared between the two groups.Results:The FEV1/FVC ratio in the experimental group(58.3±7.2)was lower than that in the healthy control group(92.1±4.8);the total CAT score(22.4±3.5)was higher than that in the healthy control group(3.1±1.2);both Vanin-1(18.7±2.3μg/L)and SPP1(25.6±4.1μg/L)levels were higher than those in the healthy control group;LAA%-950(38.7±6.2%)and WA%(68.5±5.3%)were significantly higher than those in the healthy control group(all p<0.001).Conclusion:Chest CT combined with serum Vanin-1 and SPP1 can accurately quantify the pathological progression of COPD,providing a dual basis for clinical staging and individualized intervention.展开更多
Thirty-six pathogenetic bacterial strains were isolated from wilted mulberry plants in Hangzhou,Zhejiang province of China.The six representative strains were confirmed to be involved in more than one Enterobacter spe...Thirty-six pathogenetic bacterial strains were isolated from wilted mulberry plants in Hangzhou,Zhejiang province of China.The six representative strains were confirmed to be involved in more than one Enterobacter species by common bacteriological test,electron microscope observation,hypersensitive reaction,Koch’s postulates,physiological and biochemical test,biolog,fatty acid methyl esters analysis (FAMEs),enterobacterial repetitive intergenic consensus-PCR (ERIC-PCR),16s rRNA sequences analysis,and comparative analysis with 7 type strains and 3 reference strains.This is the first report on mulberry disease caused by Enterobacter spp.in the world providing new evidence on induction of the plant disease in this genus.The results are not only important in the mulberry disease management but also have significant scientific value for further studies of opportunistic human pathogens and environmental strains in Enterobacter.展开更多
Nosocomial infections are frequent complications of hospitalization, caused by opportunistic pathogens that gain access to hosts undergoing invasive procedures, such as surgery, intubation, and placement of deep vein ...Nosocomial infections are frequent complications of hospitalization, caused by opportunistic pathogens that gain access to hosts undergoing invasive procedures, such as surgery, intubation, and placement of deep vein lines. Nosocomial infections in animal hospitals can infect other animals, as well as be transmitted to human personnel. Enterobacter is a genus of common gram-negative bacteria, which can be associated with antibiotic resistant hospital infections. Because of an outbreak in antibiotic resistance in the genus, we decided to investigate five years of Enterobacter infections in the Large Animal Services of the Lois Bates Acheson Veterinary Teaching Hospital (LBAVTH) at Oregon State University. The demographics from 37 Enterobacter-infected patients of the LBAVTH were obtained from charts and analyzed. The identified clusters of infections suggested possible patient-environment sources of infection. The environment of the hospital was sampled in an attempt to determine the source of infection. Although Enterobacter was not isolated, three of the collected samples contained bacteria with resistance to third-generation cephalosporins. Enterobacter isolates from six of the 37 patients were further analyzed for presence of specific ESBL resistance genes. All six of the isolates harbored multiple extended-spectrum beta-lactamase genes, i.e., CTX-M-15, TEM-80, SHV-2 and AmpC. In summary, Enterobacter infection in the veterinary hospital was caused by beta-lactam-resistant strains, carrying ESBL-resistant genes. Veterinary hospital personnel should be aware of the potential for transmission, to both humans and animals, of ESBL-gene-containing bacteria.展开更多
Antimicrobial resistance poses a global threat to human health.Analyzing monitoring data on antimicrobial resist-ance can assist clinicians in making strategic decisions and promptly identifying outbreaks of antimicro...Antimicrobial resistance poses a global threat to human health.Analyzing monitoring data on antimicrobial resist-ance can assist clinicians in making strategic decisions and promptly identifying outbreaks of antimicrobial-resistant organisms.The China Antimicrobial Surveillance Network(CHINET)was established in 2004 to monitor the trends in bacterial epidemiology and antimicrobial resistance.In this study,we analyzed the distribution and changing anti-microbial resistance profiles of Enterobacter spp.isolated from 53 hospitals across China between 2015 and 2021 using the CHINET data.Over the seven-year period,a total of 37,966 clinical isolates of Enterobacter spp.were obtained,accounted for 2.5%of all isolates and 5.7%of Enterobacteriaceae isolates.Among those isolates,Enterobacter cloacae was the most prevalent,comprising 93.7%(35,571/37,966).The majority of strains were isolated from respiratory tract samples(44.6%),followed by secretion,pus(16.4%),and urine samples(16.0%).As for patient composition,37,966 Enterobacter spp.strains were predominantly isolated from inpatients(92.9%),whereas 7.1%were isolated from out-patients and emergency patients.Among inpatients,isolates from patients in surgical ward accounted for the highest percentage(24.4%).E.cloacae exhibited the lowest rates of resistance to amikacin,tigecycline,polymyxin B,imipe-nem,and meropenem(resistance rates<8%).However,the percentage of carbapenem-resistant Enterobacter spp.was 10.0%,presenting a rising tendency over the 7-year study period.Antimicrobial resistance profiles of Enterobacter spp.isolates varied according to the department of isolation and patient age(adult or child),with the intensive care unit having the highest proportion of carbapenem-resistant Enterobacter spp.isolates.展开更多
Cronobacter spp.has strong resistance to desiccation and high permeability in Enterobacteriaceae,and powdered infant formula(PIF)is one of the main contamination routes.In recent years,the contamination of Cronobacter...Cronobacter spp.has strong resistance to desiccation and high permeability in Enterobacteriaceae,and powdered infant formula(PIF)is one of the main contamination routes.In recent years,the contamination of Cronobacter spp.in PIF incidents occurs from time to time,causing infant serious diseases or death.In this investigation,matrix-assisted laser desorption/ionization time of flight mass spectrometry was used to identify the phenotypes of 35 Cronobacter strains isolated from PIF and its processing environment.Subsequently,the isolates were evaluated for drying and osmotic pressure tolerance.The results showed that the deactivation rate of the strains ranged from 9.01%to 77.57%,and the highest osmotic pressure condition the strains could tolerate was 6 g/100 mL Na Cl.In addition,there was a positive correlation between biofilm formation ability and desiccation resistance.Combined with transcriptomics,Cronobacter spp.could activate biofilm synthesis,produce more trehalose,accumulate betaine and electrolytes to stabilize intracellular structure under the two treatment conditions.A total of 31 and 43 genes were found related to desiccation and permeability resistance,respectively.And some genes(cysM,thuF,ycjO,etc.)were found to be associated with two tolerances for the first time.展开更多
Objective:To evaluate the predictive value of secreted phosphoprotein 1(SPP1)gene expression for postoperative survival in patients with advanced liver cancer undergoing hepatic artery interventional chemoembolization...Objective:To evaluate the predictive value of secreted phosphoprotein 1(SPP1)gene expression for postoperative survival in patients with advanced liver cancer undergoing hepatic artery interventional chemoembolization treatment.Method:Bioinformatics methods,including gene ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway analysis,were used to identify genes related to survival prognosis in hepatocellular carcinoma(HCC)patients.A retrospective analysis of 115 advanced liver cancer patients treated between January 2016 and October 2017 was conducted.Patients were categorized into SPP1 high-expression(n=89)and low-expression groups(n=26).Additionally,115 healthy individuals served as the control group.The relationship between SPP1 expression and clinical pathological features was analyzed.A 60-month follow-up and logistic regression analysis identified risk factors affecting survival.Results:SPP1 mRNA expression was significantly higher in liver cancer patients compared to healthy controls(P<0.05).SPP1 expression levels were significantly associated with tumor size,Child-Pugh grading,lymph node metastasis,and BCLC staging(P<0.05).High SPP1 expression,along with tumor size,Child-Pugh grading,lymph node metastasis,and BCLC staging,were independent risk factors for survival(P<0.05).The 60-month survival rate was 17.39%,with a median survival of 40 months in the low-expression group versus 18 months in the high-expression group(P<0.05).Conclusion:SPP1 expression is significantly upregulated in advanced liver cancer patients and has predictive value for postoperative survival following hepatic artery chemoembolization treatment.SPP1,combined with clinical indicators such as tumor size,Child-Pugh grading,lymph node metastasis,and BCLC staging,may serve as a prognostic biomarker for interventional treatment outcomes.展开更多
文摘This work aimed to investigate the biosorption of lead by free and immobilized bacterial cell. Twenty four samples were collected from the wastewater of batteries factory in AL-Waziria district for the period from June to Sept. 2004, three species of bacteria have been isolated from this wastewater： Enterobacter agglomerans, E. gergoviae, and E. sakazakii. The results revealed a high variation in the ability of these bacteria for lead biosorption, the bacteria E. agglomerans （ES） was the most efficient compared to the other bacterial species. A laboratory plant with continuous flow system was designed for lead ions removal from standards aqueous solutions, as the cells were immobilized using： white sand, charcoal, agar and calcium alginate. However, the cells immobilized to calcium alginate were the most efficient in removal of lead ions （98%）. Present study also determined the optimal conditions for the biosorptJon of lead ions which were temperature 40℃ and pH 4 with one hour contiguity between the immobilized bacteria and solution. Different washing solutions were used fo~： recovering the lead ions that have been biosorbed on the bacterial walls, 0.1 M EDTA was the most efficient. Preferential biosorption by E. agglomerans （E5） to copper and cadmium was determined, result showed that the order of this preferential capability was： Cu 〉 Pb 〉 Cd. No significant differences were found in the biosorption of lead ions by alive and dead E. agglomerans （E5）. The designed lab plant containing bacterial cells binding on calcium alginate was able to uptake all lead ions from all samples of industrial wastewater of batteries factory.
基金supported by the National Research Foundation of Korea(2020R1F1A1074155).
文摘Recently,Prevotella spp.,a major genus of gram-negative commensal bacteria in humans,have emerged as a key microbial contributor to host metabolism due to its ability to ferment dietary fibers,produce beneficial short-chain fatty acids,and influence immune responses.However,their diversity and functional differences have created challenges for their development and therapeutic use.Recent studies have shown that specific Prevotella species,such as P.copri,P.intestinalis,and P.histicola,can strengthen gut barrier integrity and reduce metabolic imbalances.Notably,Prevotella populations can be increased through high-fiber or herbal-based treatments.Traditional herbal medicines,including fiber-rich decoctions,also demonstrate the potential to boost endogenous Prevotella communities,enhance microbial fermentation,and improve glucose and lipid balance.This perspective examines the context-dependent roles of Prevotella spp.,with emphasis on the functional heterogeneity of key species such as P.copri,suggests a framework for combining herbal modulation with species-level microbiota profiling,and outlines a research plan to explore microbe-herb synergy in treating obesity,type 2 diabetes,and related metabolic disorders.This strategy offers a new,ecology-based approach to complement standard metabolic interventions.
基金Baoding Science and Technology Plan Project(Project No.:2341ZF214)。
文摘Objective:To investigate the quantitative assessment efficacy of chest CT combined with serum Vanin-1 and SPP1 in determining the progression stage of chronic obstructive pulmonary disease(COPD).Methods:A total of 100 COPD subjects from our hospital from January 2020 to December 2023 were included and randomly divided into a healthy control group and an experimental group(50 cases each).The healthy control group underwent slow vital capacity measurement using a spirometer,while the experimental group underwent high-resolution thin-slice CT scans and serum Vanin-1 and SPP1 concentration measurements.Pulmonary function parameters,symptom burden,biomarker concentrations,and imaging characteristics were compared between the two groups.Results:The FEV1/FVC ratio in the experimental group(58.3±7.2)was lower than that in the healthy control group(92.1±4.8);the total CAT score(22.4±3.5)was higher than that in the healthy control group(3.1±1.2);both Vanin-1(18.7±2.3μg/L)and SPP1(25.6±4.1μg/L)levels were higher than those in the healthy control group;LAA%-950(38.7±6.2%)and WA%(68.5±5.3%)were significantly higher than those in the healthy control group(all p<0.001).Conclusion:Chest CT combined with serum Vanin-1 and SPP1 can accurately quantify the pathological progression of COPD,providing a dual basis for clinical staging and individualized intervention.
基金supported by the National Natural Science Foundation of China (Grant Nos. 30871655 and 30671397)National High Technology Research and Development Project (Grant No. 2006AA10A211)+1 种基金Zhejiang Provincial Agricultural Department (Grant No.ZNJF2008-97)Hangzhou Science & Technology Bureau and Hangzhou Agricultural Development Foundation (Grant No.2008-3)
文摘Thirty-six pathogenetic bacterial strains were isolated from wilted mulberry plants in Hangzhou,Zhejiang province of China.The six representative strains were confirmed to be involved in more than one Enterobacter species by common bacteriological test,electron microscope observation,hypersensitive reaction,Koch’s postulates,physiological and biochemical test,biolog,fatty acid methyl esters analysis (FAMEs),enterobacterial repetitive intergenic consensus-PCR (ERIC-PCR),16s rRNA sequences analysis,and comparative analysis with 7 type strains and 3 reference strains.This is the first report on mulberry disease caused by Enterobacter spp.in the world providing new evidence on induction of the plant disease in this genus.The results are not only important in the mulberry disease management but also have significant scientific value for further studies of opportunistic human pathogens and environmental strains in Enterobacter.
文摘Nosocomial infections are frequent complications of hospitalization, caused by opportunistic pathogens that gain access to hosts undergoing invasive procedures, such as surgery, intubation, and placement of deep vein lines. Nosocomial infections in animal hospitals can infect other animals, as well as be transmitted to human personnel. Enterobacter is a genus of common gram-negative bacteria, which can be associated with antibiotic resistant hospital infections. Because of an outbreak in antibiotic resistance in the genus, we decided to investigate five years of Enterobacter infections in the Large Animal Services of the Lois Bates Acheson Veterinary Teaching Hospital (LBAVTH) at Oregon State University. The demographics from 37 Enterobacter-infected patients of the LBAVTH were obtained from charts and analyzed. The identified clusters of infections suggested possible patient-environment sources of infection. The environment of the hospital was sampled in an attempt to determine the source of infection. Although Enterobacter was not isolated, three of the collected samples contained bacteria with resistance to third-generation cephalosporins. Enterobacter isolates from six of the 37 patients were further analyzed for presence of specific ESBL resistance genes. All six of the isolates harbored multiple extended-spectrum beta-lactamase genes, i.e., CTX-M-15, TEM-80, SHV-2 and AmpC. In summary, Enterobacter infection in the veterinary hospital was caused by beta-lactam-resistant strains, carrying ESBL-resistant genes. Veterinary hospital personnel should be aware of the potential for transmission, to both humans and animals, of ESBL-gene-containing bacteria.
文摘Antimicrobial resistance poses a global threat to human health.Analyzing monitoring data on antimicrobial resist-ance can assist clinicians in making strategic decisions and promptly identifying outbreaks of antimicrobial-resistant organisms.The China Antimicrobial Surveillance Network(CHINET)was established in 2004 to monitor the trends in bacterial epidemiology and antimicrobial resistance.In this study,we analyzed the distribution and changing anti-microbial resistance profiles of Enterobacter spp.isolated from 53 hospitals across China between 2015 and 2021 using the CHINET data.Over the seven-year period,a total of 37,966 clinical isolates of Enterobacter spp.were obtained,accounted for 2.5%of all isolates and 5.7%of Enterobacteriaceae isolates.Among those isolates,Enterobacter cloacae was the most prevalent,comprising 93.7%(35,571/37,966).The majority of strains were isolated from respiratory tract samples(44.6%),followed by secretion,pus(16.4%),and urine samples(16.0%).As for patient composition,37,966 Enterobacter spp.strains were predominantly isolated from inpatients(92.9%),whereas 7.1%were isolated from out-patients and emergency patients.Among inpatients,isolates from patients in surgical ward accounted for the highest percentage(24.4%).E.cloacae exhibited the lowest rates of resistance to amikacin,tigecycline,polymyxin B,imipe-nem,and meropenem(resistance rates<8%).However,the percentage of carbapenem-resistant Enterobacter spp.was 10.0%,presenting a rising tendency over the 7-year study period.Antimicrobial resistance profiles of Enterobacter spp.isolates varied according to the department of isolation and patient age(adult or child),with the intensive care unit having the highest proportion of carbapenem-resistant Enterobacter spp.isolates.
基金supported by the Joint Funds of the National Natural Science Foundation of China(U21A20272)。
文摘Cronobacter spp.has strong resistance to desiccation and high permeability in Enterobacteriaceae,and powdered infant formula(PIF)is one of the main contamination routes.In recent years,the contamination of Cronobacter spp.in PIF incidents occurs from time to time,causing infant serious diseases or death.In this investigation,matrix-assisted laser desorption/ionization time of flight mass spectrometry was used to identify the phenotypes of 35 Cronobacter strains isolated from PIF and its processing environment.Subsequently,the isolates were evaluated for drying and osmotic pressure tolerance.The results showed that the deactivation rate of the strains ranged from 9.01%to 77.57%,and the highest osmotic pressure condition the strains could tolerate was 6 g/100 mL Na Cl.In addition,there was a positive correlation between biofilm formation ability and desiccation resistance.Combined with transcriptomics,Cronobacter spp.could activate biofilm synthesis,produce more trehalose,accumulate betaine and electrolytes to stabilize intracellular structure under the two treatment conditions.A total of 31 and 43 genes were found related to desiccation and permeability resistance,respectively.And some genes(cysM,thuF,ycjO,etc.)were found to be associated with two tolerances for the first time.
基金Medical Research Project of Xi’an Science and Technology Bureau“Molecular Mechanism of miR-1305 Competitive Endogenous circRNA in the Development of Liver Cancer”(Project No.22YXYJ0134)General Project of Key Research and Development Program of Shaanxi Provincial Department of Science and Technology“Mechanism Study on the Inhibition of Liver Cancer Invasion and Metastasis by Downregulating METTL3 and Reducing the m6A Modification Level of MMP3 with Honokiol”(Project No.2023-YBSF-631)。
文摘Objective:To evaluate the predictive value of secreted phosphoprotein 1(SPP1)gene expression for postoperative survival in patients with advanced liver cancer undergoing hepatic artery interventional chemoembolization treatment.Method:Bioinformatics methods,including gene ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway analysis,were used to identify genes related to survival prognosis in hepatocellular carcinoma(HCC)patients.A retrospective analysis of 115 advanced liver cancer patients treated between January 2016 and October 2017 was conducted.Patients were categorized into SPP1 high-expression(n=89)and low-expression groups(n=26).Additionally,115 healthy individuals served as the control group.The relationship between SPP1 expression and clinical pathological features was analyzed.A 60-month follow-up and logistic regression analysis identified risk factors affecting survival.Results:SPP1 mRNA expression was significantly higher in liver cancer patients compared to healthy controls(P<0.05).SPP1 expression levels were significantly associated with tumor size,Child-Pugh grading,lymph node metastasis,and BCLC staging(P<0.05).High SPP1 expression,along with tumor size,Child-Pugh grading,lymph node metastasis,and BCLC staging,were independent risk factors for survival(P<0.05).The 60-month survival rate was 17.39%,with a median survival of 40 months in the low-expression group versus 18 months in the high-expression group(P<0.05).Conclusion:SPP1 expression is significantly upregulated in advanced liver cancer patients and has predictive value for postoperative survival following hepatic artery chemoembolization treatment.SPP1,combined with clinical indicators such as tumor size,Child-Pugh grading,lymph node metastasis,and BCLC staging,may serve as a prognostic biomarker for interventional treatment outcomes.
