摘要
【目的】筛选具有缓解盐碱胁迫潜力的野生稻源高效内生细菌,并评估所筛得菌株(Enterobacter hormaechei QT2)对盐碱胁迫下栽培稻生长、生理及产量的影响,为盐碱地水稻稳产增效及抗逆菌剂开发提供理论依据。【方法】从野生稻叶片分离内生菌E. hormaechei QT2,通过盆栽试验(中重度盐碱土)及田间验证(pH 9.3,电导率1 262μS/cm),分析其促生特性及对栽培稻的增产效应。【结果】盐碱胁迫下,接种QT2显著(P<0.05)改善了栽培稻的生长表型:株高、根长、鲜重和干重较未接菌对照分别增加101.0%、46.1%、137.4%和447.0%。同时,栽培稻叶片中渗透调节物质显著积累,可溶性糖、可溶性蛋白和脯氨酸含量分别提升135.7%、94.0%和205.2%,丙二醛(MDA)含量降低59.6%,膜稳定性(MSI)提高42.3%;栽培稻叶片抗氧化系统活性显著增强,超氧化物歧化酶(SOD)、过氧化物酶(POD)活性及谷胱甘肽(GSH)含量分别提高57.5%、73.3%和39.4%;栽培稻叶片光合作用增强,叶绿素a、叶绿素b、类胡萝卜素含量分别提高了30.69%、78.57%、75.62%。此外,菌株QT2还表现出多种植物促生长特性(固氮、溶磷、解钾、产IAA、产ACC脱氨酶及产氨)。田间试验证实,接种QT2可使盐碱地栽培稻产量提高29.5%,菌剂QT2投入产出比达1∶46。【结论】内生细菌E. hormaechei QT2通过协同调控渗透平衡、增强抗氧化防御、提升光合效率等机制,有效缓解盐碱胁迫对栽培稻的抑制作用,显著促进其生长并提高产量。
【Objective】Screening wild rice-derived endophytic bacteria with high potential for mitigating salinity-alkalinity stress,and evaluating the effects of Enterobacter hormaechei QT2 on the growth,physiology,and yield of cultivated rice under such stress conditions.This study aims to provide a theoretical basis for stabilizing rice yields and enhancing efficiency in saline-alkali lands,as well as for developing stress-resistant microbial agents.【Method】The endophytic bacterium E.hormaechei QT2 was isolated from the leaves of wild rice,and its growth-promoting characteristics and yield-enhancing effects were tested in a pot experiment(middle-heavy saline-alkaline soil)and a field validation(pH 9.3,conductivity 1262μS/cm).【Result】Under saline-alkali stress,inoculation of QT2 significantly improved the growth phenotype of cultivated rice(P<0.05),demonstrated the increase of plant height,root length,fresh weight and dry weight increased by 101.0%,46.1%,137.4%and 447.0%,respectively.Meanwhile,the osmotically regulated substances were significantly accumulated in the leaves of cultivated rice,and the contents of soluble sugars,soluble proteins and proline increased by 135.7%,94.0%and 205.2%,respectively.The content of malondialdehyde(MDA)reduced by 59.6%,and the membrane stability index(MSI)increased by 42.3%.The activity of antioxidant system in the leaves of the cultivated rice was significantly enhanced,and the activities of superoxide dismutase(SOD),peroxidase(POD)and the content of glutathione(GSH)increased by 57.5%,73.3%and 39.4%respectively.The photosynthesis of cultivated rice leaves was enhanced,and the contents of chlorophyll a,chlorophyll b and carotenoids increased by 30.69%,78.57%and 75.62%,respectively.In addition,strain QT2 also showed a variety of plant growth-promoting properties(nitrogen fixation,phosphorus solubilization,potassium solubilization,IAA production,ACC deaminase production and ammonia production).Field experiments further confirmed that inoculation with QT2 increased the yield of saline cultivated rice by 29.5%,and the input-output ratio of strain QT2 reached 1:46.【Conclusion】The endophytic bacterium E.hormaechei QT2 effectively alleviates the inhibitory effects of saline-alkali stress on cultivated rice by synergistically regulating osmotic balance,enhancing antioxidant defense,and improving photosynthetic efficiency,thereby significantly promoting growth and increasing yield.
作者
李俊辰
赵湘媛
汪福东
王一凡
张建峰
汪树生
LI Jun-chen;ZHAO Xiang-yuan;WANG Fu-dong;WANG Yi-fan;ZHANG Jian-feng;WANG Shu-sheng(Key Laboratory of Straw Comprehensive Utilization and Black Soil Conservation,College of Life Sciences,Jilin Agricultural University,Changchun 130118;National Engineering and Technology Research Center for Deep Processing of Wheat and Corn,School of Food Science and Engineering,Jilin Agricultural University,Changchun 130118;Key Laboratory of Agro-ecology in Black Soil Region,Northeast Institute of Geography and Agro-ecology,Chinese Academy of Sciences,Changchun 130102)
出处
《生物技术通报》
北大核心
2025年第12期106-113,共8页
Biotechnology Bulletin
基金
吉林省科技发展项目(20220202022NC)。
关键词
野生稻
内生细菌
贺氏肠杆菌
栽培稻
盐碱胁迫
wild rice
endophytic bacteria
Enterobacter hormaechei
cultivated rice
saline-alkali stress
