A novel matrix of macropore cellulose membrane was prepared by chemical graft, and immobilized the cationic charged groups as affinity ligands. The prepared membrane Fan be used for the removal of endotoxin from human...A novel matrix of macropore cellulose membrane was prepared by chemical graft, and immobilized the cationic charged groups as affinity ligands. The prepared membrane Fan be used for the removal of endotoxin from human serum albumin (HSA) solutions. With a cartridge of 20 sheets affinity membrane of 47 mm diameter, the endotoxin level in HSA solution can be reduced ro 0.027 eu/mL. Recovery of HSA was over 95%.展开更多
Affinity membrane was prepared with chitosan immobilized on the hydrophile- modified poly(vinylidene fluoride) (PVDF) membrane. Fourier transform infrared spectroscopy (FTIR.) analysis indicated that the contents of ...Affinity membrane was prepared with chitosan immobilized on the hydrophile- modified poly(vinylidene fluoride) (PVDF) membrane. Fourier transform infrared spectroscopy (FTIR.) analysis indicated that the contents of —NH2 and —OH groups increased and fluoride decreased on the membrane surface after modification. Using this kind of affinity membrane, the effects of operation parameters such as pH, ionic strength and flow rate, on the amount of endotoxin removed were investigated. The results showed that the equilibrium adsorption capacity and the dissociation constant of the affinity membrane to endotoxin were 21.4 EU·mg-1 membrane and 0.50EU·ml-1, respectively, at pH 7.0 and ionic strength 0.2 mol·L-1. Adsorption appeared to follow a typical Langmuir adsorption isotherm. At pH 5.0, ionic strength of 0.2 mol·L-1, the removal rate of endotoxin from BSA solution with the chitosan affinity membrane was up to 88.6% (11.50 EU·mg-1 membrane), and the recovery of BSA was 93.4% (0.187mg·mg-1 membrane), while at pH 11.0, ionic strength of 0.2mol·L-1, the removal rate of endotoxin from lysozyme solution was 72.4% (9.92EU·mg-1 membrane), and the recovery of lysozyme was 92.3% (0.104 mg·mg-1 membrane).展开更多
OBJECTIVE:To evaluate the efficacy and safety of activating blood circulation and removing blood stasis in terms of Traditional Chinese Medicine(TCM) for managing renal fibrosis(RF) in patients with chronic kidney dis...OBJECTIVE:To evaluate the efficacy and safety of activating blood circulation and removing blood stasis in terms of Traditional Chinese Medicine(TCM) for managing renal fibrosis(RF) in patients with chronic kidney disease(CKD).METHODS:We searched randomized controlled trials(RCTs) from eight databases.RESULTS:Sixteen eligible studies with 1,356 participants were included in this study.Compared to treatment with Western Medicine(WM) alone,the combined treatment with activating blood circulation and removing blood stasis in terms of TCM(ARTCM) and WM to manage RF in patients with CKD significantly ameliorated type Ⅳ collagen(C-Ⅳ)(SMD:-2.17,95% CI:3.01 to-1.34),type Ⅲ procollagen(PC-Ⅲ)(SMD:-1.08,95% CI:-1.64 to-0.53),laminin(LN)(SMD:-1.28,95% CI:-1.65 to-0.90),transforming growth factor β 1(TGF-β1)(SMD:-0.65,95% CI:-1.18 to-0.12),serum creatinine(Scr)(SMD:-1.36,95% CI:-1.85 to-0.87),blood urea nitrogen(BUN)(MD:-1.51,95% CI:-2.59 to-0.43),and 24 h urine protein(24h Upro)(SMD:-1.23;95% CI:-1.96 to-0.50).The level of hyaluronic acid(HA) was similar in both types of treatment(SMD:-0.74,95% CI:-1.91 to 0.44).The subgroup analysis showed that the duration of 8 weeks might affect the concentration of C-Ⅳ,PC-Ⅲ,and LN(P < 0.05).The effectiveness of the longer duration to C-Ⅳ,PC-Ⅲ,and LN was not certain.However,the result should be interpreted in care.The safety of the treatment using ARTCM and WM could not be evaluated because a few studies had reported adverse effects.The results of the Meta-analysis were not stable enough.There was publication bias for the reports on Scr(P = 0.001),C-Ⅳ(P = 0.001),PC-Ⅲ(P = 0.026),and LN(P = 0.030) and no publication bias for the reports on BUN(P = 0.293).The quality of evidence varied from low to very low.CONCLUSIONS:The combined treatment using ARTCM and WM to manage RF in patients with CKD has some advantages over treatment with WM alone.High-quality RCTs need to be conducted for the strong support.展开更多
Sepsis is responsible for approximately 5.3 million deaths globally each year.Here,we constructed hierarchical chitin microspheres loaded with MOF-919(Ch/metal–organic frameworks[MOFs])for the rapid and efficient rem...Sepsis is responsible for approximately 5.3 million deaths globally each year.Here,we constructed hierarchical chitin microspheres loaded with MOF-919(Ch/metal–organic frameworks[MOFs])for the rapid and efficient removal of lipopolysaccharide(LPS)in complex blood environments.Furthermore,abun-dant active sites on MOF-919(Sc)also enable a record-high adsorption capacity of 9.56 mg/g in biomass-based adsorbents due to the coordination interactions between endotoxin and MOF-919(Sc).The LPS level of sepsis rabbits was less than 2 EU/mL(clearance rate>95%)after 90-min hemoperfusion,showing no adverse effect on the rabbit organs.Additionally,compared to the commonly used LPS scrubber Toraymyxin(polymethyl methacrylate),the chitin adsorbent is significantly more cost-effective and environmentally friendly.The prepara-tion strategy for hierarchical porous microspheres offers notable advantages in designability,recyclability,and renewability,providing a new approach to sepsis treatment and promising prospects for the biomedical application of sustainable biomass materials.展开更多
Intracerebral hemorrhage(ICH) is a significant and growing threat to human health,with increasing incidence.Promoting blood circulation and removing blood stasis therapy(PBCRBS),a Traditional Chinese Medicine therapy,...Intracerebral hemorrhage(ICH) is a significant and growing threat to human health,with increasing incidence.Promoting blood circulation and removing blood stasis therapy(PBCRBS),a Traditional Chinese Medicine therapy,can be an adjuvant therapy to benefit patients with ICH by improving clinical efficacy.However,in theory,using PBCRBS to treat ICH carries the risk of hematoma enlargement and rebleeding,which has led to controversy over its application in ICH treatment.To demonstrate the effectiveness and safety of PBCRBS in treating ICH,this review first analyzes the pathological and physiological basis of ICH and secondly,the cascade of response after ICH and the involvement of cytokines and signaling pathways in this process.Finally,experimental and clinical studies on the treatment of ICH with PBCRBS over the past decade were retrieved from the Pub Med and China National Knowledge Infrastructure databases,and the content of these studies was used to summarize commonly used herbs with PBCRBS effects and their mechanisms of action.Through analysis,hypertension has been identified as the most common cause of ICH.Heme,interleukin,reactive oxygen species,coagulation promoting particles and other induced mass effects,inflammation,oxidative stress,and coagulation cascade reactions lead to brain damage following ICH.This review includes 56 experimental studies and 83 clinical studies summarizing 28 commonly used herbs,demonstrating the positive impact of PBCRBS as an adjuvant therapy for ICH.In summary,PBCRBS appears effective and safe for treating ICH.展开更多
Endotoxins(also known as lipopolysaccharides(LPS)) are undesirable by-products of recombinant proteins,purified from Escherichia coli.LPS can be considered stable under a wide range of temperature and pH,making their ...Endotoxins(also known as lipopolysaccharides(LPS)) are undesirable by-products of recombinant proteins,purified from Escherichia coli.LPS can be considered stable under a wide range of temperature and pH,making their removal one of the most difficult tasks in downstream processes during protein purification.The inherent toxicity of LPS makes their removal an important step for the application of these proteins in several biological assays and for a safe parenteral administration.Immobilized metal affinity chromatography(IMAC) enables the affinity interactions between the metal ions(immobilized on the support through the chelating compound) and the target molecules,thus enabling high-efficiency separation of the target molecules from other components present in a mixture.Affinity chromatography is applied with Ca2+-iminodiacetic acid(IDA) to remove most of the LPS contaminants from the end product(more than90%).In this study,the adsorption of LPS on an IDA-Ca2+ was investigated.The adsorption Freundlich isotherm of LPS-IDA-Ca2+ provides a theoretical basis for LPS removal.It was found that LPS is bound mainly by interactions between the phosphate group in LPS and Ca2+ ligands on the beads.The factors such as pH(4.0 or 5.5) and ionic strength(1.0 mol/L) are essential to obtain effective removal of LPS for contaminant levels between endotoxin' concentration values less than100 EU/mL and 100 000 EU/mL.This new protocol represents a substantial advantage in time,effort,and production costs.展开更多
文摘A novel matrix of macropore cellulose membrane was prepared by chemical graft, and immobilized the cationic charged groups as affinity ligands. The prepared membrane Fan be used for the removal of endotoxin from human serum albumin (HSA) solutions. With a cartridge of 20 sheets affinity membrane of 47 mm diameter, the endotoxin level in HSA solution can be reduced ro 0.027 eu/mL. Recovery of HSA was over 95%.
基金Supported by the National Basic Research Program of China (No. 2003CB615706)
文摘Affinity membrane was prepared with chitosan immobilized on the hydrophile- modified poly(vinylidene fluoride) (PVDF) membrane. Fourier transform infrared spectroscopy (FTIR.) analysis indicated that the contents of —NH2 and —OH groups increased and fluoride decreased on the membrane surface after modification. Using this kind of affinity membrane, the effects of operation parameters such as pH, ionic strength and flow rate, on the amount of endotoxin removed were investigated. The results showed that the equilibrium adsorption capacity and the dissociation constant of the affinity membrane to endotoxin were 21.4 EU·mg-1 membrane and 0.50EU·ml-1, respectively, at pH 7.0 and ionic strength 0.2 mol·L-1. Adsorption appeared to follow a typical Langmuir adsorption isotherm. At pH 5.0, ionic strength of 0.2 mol·L-1, the removal rate of endotoxin from BSA solution with the chitosan affinity membrane was up to 88.6% (11.50 EU·mg-1 membrane), and the recovery of BSA was 93.4% (0.187mg·mg-1 membrane), while at pH 11.0, ionic strength of 0.2mol·L-1, the removal rate of endotoxin from lysozyme solution was 72.4% (9.92EU·mg-1 membrane), and the recovery of lysozyme was 92.3% (0.104 mg·mg-1 membrane).
基金Supported by the Fund of Science and Technology Sichuan Province:Optimization and Evaluation of Clinical Scheme of Traditional Chinese Medicine for Delaying the Progression of Chronic Kidney Disease by Fuzhenghuayu Jiangzhuo tongluo Principle(No.2021YFS0034)the National Natural Science Foundation of China:Studies on Mechanism of Bupiyishen Tongfu Xiezhuo Principle in Delaying the Progression of Chronic Kidney Disease in Rats by Adjusting Host Metabonomics and Gut Microbiota with Multi-omics Technology(No.81973673)。
文摘OBJECTIVE:To evaluate the efficacy and safety of activating blood circulation and removing blood stasis in terms of Traditional Chinese Medicine(TCM) for managing renal fibrosis(RF) in patients with chronic kidney disease(CKD).METHODS:We searched randomized controlled trials(RCTs) from eight databases.RESULTS:Sixteen eligible studies with 1,356 participants were included in this study.Compared to treatment with Western Medicine(WM) alone,the combined treatment with activating blood circulation and removing blood stasis in terms of TCM(ARTCM) and WM to manage RF in patients with CKD significantly ameliorated type Ⅳ collagen(C-Ⅳ)(SMD:-2.17,95% CI:3.01 to-1.34),type Ⅲ procollagen(PC-Ⅲ)(SMD:-1.08,95% CI:-1.64 to-0.53),laminin(LN)(SMD:-1.28,95% CI:-1.65 to-0.90),transforming growth factor β 1(TGF-β1)(SMD:-0.65,95% CI:-1.18 to-0.12),serum creatinine(Scr)(SMD:-1.36,95% CI:-1.85 to-0.87),blood urea nitrogen(BUN)(MD:-1.51,95% CI:-2.59 to-0.43),and 24 h urine protein(24h Upro)(SMD:-1.23;95% CI:-1.96 to-0.50).The level of hyaluronic acid(HA) was similar in both types of treatment(SMD:-0.74,95% CI:-1.91 to 0.44).The subgroup analysis showed that the duration of 8 weeks might affect the concentration of C-Ⅳ,PC-Ⅲ,and LN(P < 0.05).The effectiveness of the longer duration to C-Ⅳ,PC-Ⅲ,and LN was not certain.However,the result should be interpreted in care.The safety of the treatment using ARTCM and WM could not be evaluated because a few studies had reported adverse effects.The results of the Meta-analysis were not stable enough.There was publication bias for the reports on Scr(P = 0.001),C-Ⅳ(P = 0.001),PC-Ⅲ(P = 0.026),and LN(P = 0.030) and no publication bias for the reports on BUN(P = 0.293).The quality of evidence varied from low to very low.CONCLUSIONS:The combined treatment using ARTCM and WM to manage RF in patients with CKD has some advantages over treatment with WM alone.High-quality RCTs need to be conducted for the strong support.
基金National Natural Science Foundation of China,Grant/Award Number:22075215Key Research and Development Program of Hubei Province,Grant/Award Number:2021BCA122+1 种基金Translational Medicine and Interdisciplinary Research Joint Fund of Zhongnan Hospital of Wuhan University,Grant/Award Number:ZNJC202238Fundamental Research Funds for the Central Universities,Grant/Award Number:691000003。
文摘Sepsis is responsible for approximately 5.3 million deaths globally each year.Here,we constructed hierarchical chitin microspheres loaded with MOF-919(Ch/metal–organic frameworks[MOFs])for the rapid and efficient removal of lipopolysaccharide(LPS)in complex blood environments.Furthermore,abun-dant active sites on MOF-919(Sc)also enable a record-high adsorption capacity of 9.56 mg/g in biomass-based adsorbents due to the coordination interactions between endotoxin and MOF-919(Sc).The LPS level of sepsis rabbits was less than 2 EU/mL(clearance rate>95%)after 90-min hemoperfusion,showing no adverse effect on the rabbit organs.Additionally,compared to the commonly used LPS scrubber Toraymyxin(polymethyl methacrylate),the chitin adsorbent is significantly more cost-effective and environmentally friendly.The prepara-tion strategy for hierarchical porous microspheres offers notable advantages in designability,recyclability,and renewability,providing a new approach to sepsis treatment and promising prospects for the biomedical application of sustainable biomass materials.
基金National Natural Science Foundation of China:Study on the Intervention Mechanism of Zhilong Huoxue Tongyu Capsule in Hypertensive Myocardial Fibrosis by Regulating Transforming Growth Factor-β/Smad3/Erbb4-IR/miR29b Loop (No.82074378)Project of Science & Technology Department of Sichuan Province:Research on the Optimization of Basic and Clinical Plans for Treating Coronary Heart Disease and Heart Failure based on Xuanfu Theory (No.2022YFS0618)+1 种基金Luzhou Science and Technology Bureau Project:the Mechanism of Hirudin Inhibiting AngⅡInduced Myocardial Hypertrophy by Regulating Autophagy and NOD-like Receptor Family Pyrin Domain Containing Protein 3 Inflammasome (No.2023JYJ029)Southwest Medical University Project:Study on the Mechanism of Zhilong Huoxue Tongyu Capsules in Improving NOD-like Receptor Family Pyrin Domain Containing Protein 3 Inflammasome Mediated Cardiomyocyte Pyroptosis in Heart Failure by Regulating the HippoYes-Associated Protein Pathway (No.2023ZYYQ04)。
文摘Intracerebral hemorrhage(ICH) is a significant and growing threat to human health,with increasing incidence.Promoting blood circulation and removing blood stasis therapy(PBCRBS),a Traditional Chinese Medicine therapy,can be an adjuvant therapy to benefit patients with ICH by improving clinical efficacy.However,in theory,using PBCRBS to treat ICH carries the risk of hematoma enlargement and rebleeding,which has led to controversy over its application in ICH treatment.To demonstrate the effectiveness and safety of PBCRBS in treating ICH,this review first analyzes the pathological and physiological basis of ICH and secondly,the cascade of response after ICH and the involvement of cytokines and signaling pathways in this process.Finally,experimental and clinical studies on the treatment of ICH with PBCRBS over the past decade were retrieved from the Pub Med and China National Knowledge Infrastructure databases,and the content of these studies was used to summarize commonly used herbs with PBCRBS effects and their mechanisms of action.Through analysis,hypertension has been identified as the most common cause of ICH.Heme,interleukin,reactive oxygen species,coagulation promoting particles and other induced mass effects,inflammation,oxidative stress,and coagulation cascade reactions lead to brain damage following ICH.This review includes 56 experimental studies and 83 clinical studies summarizing 28 commonly used herbs,demonstrating the positive impact of PBCRBS as an adjuvant therapy for ICH.In summary,PBCRBS appears effective and safe for treating ICH.
基金supported by grants from the Brazilian Agency Coordination of Graduate Level Training(CAPES,project 0366/09-9)State of So Paulo Research Support Foundation(FAPESP-Brazil,project 2005/60159-7)
文摘Endotoxins(also known as lipopolysaccharides(LPS)) are undesirable by-products of recombinant proteins,purified from Escherichia coli.LPS can be considered stable under a wide range of temperature and pH,making their removal one of the most difficult tasks in downstream processes during protein purification.The inherent toxicity of LPS makes their removal an important step for the application of these proteins in several biological assays and for a safe parenteral administration.Immobilized metal affinity chromatography(IMAC) enables the affinity interactions between the metal ions(immobilized on the support through the chelating compound) and the target molecules,thus enabling high-efficiency separation of the target molecules from other components present in a mixture.Affinity chromatography is applied with Ca2+-iminodiacetic acid(IDA) to remove most of the LPS contaminants from the end product(more than90%).In this study,the adsorption of LPS on an IDA-Ca2+ was investigated.The adsorption Freundlich isotherm of LPS-IDA-Ca2+ provides a theoretical basis for LPS removal.It was found that LPS is bound mainly by interactions between the phosphate group in LPS and Ca2+ ligands on the beads.The factors such as pH(4.0 or 5.5) and ionic strength(1.0 mol/L) are essential to obtain effective removal of LPS for contaminant levels between endotoxin' concentration values less than100 EU/mL and 100 000 EU/mL.This new protocol represents a substantial advantage in time,effort,and production costs.
