Two new triterpenoid glucosides ecliptasaponin A(3)and ecliptasaponin B(4)were isolated together with echinocystic acid(1)and oleanolic acid(2)from Eclipta alba(L.) Hassk.Their structures were deduced as 3β,16a-dihyd...Two new triterpenoid glucosides ecliptasaponin A(3)and ecliptasaponin B(4)were isolated together with echinocystic acid(1)and oleanolic acid(2)from Eclipta alba(L.) Hassk.Their structures were deduced as 3β,16a-dihydroxy olean-12-ene-28-oic acid-3β-O-β-D-glu-copyranoside(3)and 3β-O-[β-D-glucopyranosyl(1-4)]-β-D-glucopyranosyl-16a-hydroxy olean-12-ene-28-oic acid 28-O-β-D-glucopyranoside(4),based on spectral analysis and chemical evidences as well as results ofhydrolysis.展开更多
Five triterpenoid saponins were isolated from the Chinese traditional medicine Eclipta prostrata L.. On the basis of their chemical properties and spectral data, they were identified as eclalbasaponins II(1), I(2), II...Five triterpenoid saponins were isolated from the Chinese traditional medicine Eclipta prostrata L.. On the basis of their chemical properties and spectral data, they were identified as eclalbasaponins II(1), I(2), III(3), 3-O-[b-D-glucopyranosyl(12)-b-D-glucopyranosyl]-16a-ethoxy-olean-12-ene-28-oic acid-28-O-b-D-glu-copyranoside(4) and 3-O-[(2-O-sulfuryl-b-D-glucopyranosyl)(12)-b-D-glucopyranosyl]-echinocystic acid-28-O-b-D-glucopyranoside(5). Compounds 4 and 5 are new compounds and named eclalbasaponins XI and XII, respectively. Compounds 1 and 5 induced morphological deformation of Pyricularia oryzae mycelia.展开更多
Objective:The present study deals with the investigation of larvicidal and ovicidal activities of benzene,hexane,ethyl acetate,methanol and chloroform leaf extract of Eclipta alba（E.alba） against dengue vector,Aedes...Objective:The present study deals with the investigation of larvicidal and ovicidal activities of benzene,hexane,ethyl acetate,methanol and chloroform leaf extract of Eclipta alba（E.alba） against dengue vector,Aedes aegypti（Ae.Aegypti）.Methods:Twenty five earlyⅢinstar larvae of Ae.aegypti was exposed to various concentrations（50-300 ppm） and was assayed in the laboratory by using the protocol of WHO 2005;the 24 h LC<sub>50</sub> values of the E.alba leaf extract was determined by Probit analysis.For ovicidal activity,slightly modified method of Su and Mulla was performed.The ovicidal activity was determined against Ae.aegypti to various concentrations ranging from 100-350 ppm under the laboratory conditions.The egg hatch rates were assessed 48 h post treatment.Results:The LC<sub>50</sub> values of benzene,hexane,ethyl acetate,methanol and chloroform extract of E.alba against early third instar larvae of Ae.aegypti were 151.38,165.10, 154.88,127.64 and 146.28 ppm,respectively.Maximum larvicidal activity was observed in the methanol extract followed by chloroform,benzene,ethyl acetate and hexane extract.No mortality was observed in control.Among five solvent tested the methanol extract was found to be most effective for ovicidal activity against Ae.aegypti.The methanol extracts exerted 100%mortality （zero hatchability） at 300 ppm.Conclusions:From the results it can be concluded the crude extract of E.alba was an excellent potential for controlling Ae.aegypti mosquito.展开更多
OBJECTIVE: To investigate the role of Eclipta prostrata (E. prostrata) extract in improving spatial learning and memory deficits in D-galactose-induced aging in rats. METHODS: Rats were divided into five groups, with ...OBJECTIVE: To investigate the role of Eclipta prostrata (E. prostrata) extract in improving spatial learning and memory deficits in D-galactose-induced aging in rats. METHODS: Rats were divided into five groups, with 10 animals in each group. Aging rats were produced by treatment with 100 mg·kg-1·d-1 of D-galactose for 6 weeks. Rats in the E. prostrata treatment groups received an aqueous extract of E. prostrata orally at a concentration of 50, 100, or 200 mg·kg-1· d-1 for 3 weeks. Animals in both the normal and model groups were treated with similar volumes of saline. Spatial memory performance was measured using the Morris water maze. The mRNA levels and enzyme activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and glutathione reductase (GR) were analyzed using real- time quantitative PCR and spectrophotometry,respectively. The levels of induced nitric oxide synthase (iNOS), nitric oxide (NO), dopamine (DA), norepinephrine (NE), and serotonin (5-HT) were determined using enzyme-linked immunosorbent assay and spectrophotometry. RESULTS: Compared with the normal group, rats in the D-galactose-treated model group exhibited significant memory loss. There was severe damage to the hippocampal CA1 area, and expression levels of SOD, CAT, GPx, and GR were significantly decreased in the model group compared with the normal group. In the model group, levels of iNOS and NO were significantly increased compared with the normal group. However, treatment with E. prostrata extract reversed the conditions caused by D-galactose- induced aging, especially in the groups with higher treatment concentrations. Compared with the normal group, the levels of DA, NE, and 5-HT were significantly lower in the D-galactose-treated model group. In the E. prostrata extract-treated groups, however, there was a dose-dependent upregulation of DA, NE, and 5-HT expression. CONCLUSION: Our results suggest that administration of E. prostrata extract can result in an improvement in the learning and memory impairments that are induced by D-galactose treatment in rats. This improvement may be the result of enhanced antioxidative ability, decreased iNOS and NO levels, and the induction of DA, NE, and 5-HT expression in the brain.展开更多
Due to ease of application, cost effectiveness and environmentally safe, in this study, the corrosion inhibition effect of aqueous extract of Eclipta alba in 1N hydrochloric acid has been investigated by weight loss, ...Due to ease of application, cost effectiveness and environmentally safe, in this study, the corrosion inhibition effect of aqueous extract of Eclipta alba in 1N hydrochloric acid has been investigated by weight loss, potentiodynamic polarization and impedance methods and the extracts of Eclipta alba were found to be effective corrosion pickling inhibitor. The effect of immersion time and temperature revealed that the extracts of Eclipta alba with an optimum concentration of 8.0% v/v showed maximum inhibition efficiency of 99.6% at 3 h immersion time and 30℃. Arrhenius plots for mild steel immersed in 1N HCl solution in the absence and presence of optimum concentration （8.0% in v/v） of Eclipta alba extract showed the effect of temperature. Polarization studies indicate that this plant extract acts as a mixed type inhibitor. The adsorption of Eclipta alba follows Langmuir adsorption isotherm. The inhibition action may be due to the presence of the Wedelactone and also the alkaloid Ecliptine present in the leaves of Eclipta alba.展开更多
Objective:To evaluate the effects of wedelolactone,a major flavonoid from Vietnamese Eclipta prostrata(L)L.,on inflammation and insulin resistance.Methods:Wedelolactone was extracted from the leaves of Vietnamese Ecli...Objective:To evaluate the effects of wedelolactone,a major flavonoid from Vietnamese Eclipta prostrata(L)L.,on inflammation and insulin resistance.Methods:Wedelolactone was extracted from the leaves of Vietnamese Eclipta prostrata(L.)L.with methanol by Soxhlet.The effects of wedelolactone on lipopolysaccharide(LPS)-induced cytokine production,reactive oxygen species(ROS)generation,and nicotinamide adenine dinucleotide phosphate(NADPH)oxidase activities in Raw 264.7 cells were measured by enzyme-linked immunosorbent assay(ELISA),specific immunofluorescent dyes and luminometric analysis,respectively.In addition,its effects on glucose uptake and the protein expression of insulin receptor substrate 1(IRS1)and glucose transporter 4(GLUT4)were examined in 3T3-L1 cells by immunofluorescent dyes and Western blot.Results:Wedelolactone at 30μg/mL significantly inhibited LPS-induced production of tumor necrosis factor-α,interleukin(IL)-6,and IL-8(P<0.01)with no noticeable effects on IL-10 level.It also reduced ROS generation and NADPH oxidase activities in LPS-stimulated Raw 264.7 cells(P<0.01).Furthermore,wedelolactone showed anti-insulin resistance activity,as evidenced by improved glucose uptake and the upregulated expression of IRS1 and GLUT4 in 3T3-L1 cells(P<0.01).Conclusions:Wedelolactone exhibits anti-inflammation and anti-insulin resistance effects,which may be used for the treatment of diabetes and inflammation-associated diseases.展开更多
Eclipta prostrata has been extensively studied for its promoting effect on human health. Previous study proved that E. prostrata possessed anti-osteoporosis function in SAMP6 mice via gut microbiota (GM). Endophytes a...Eclipta prostrata has been extensively studied for its promoting effect on human health. Previous study proved that E. prostrata possessed anti-osteoporosis function in SAMP6 mice via gut microbiota (GM). Endophytes are widespread in plants, especially in Chinese herbal medicines. However, little is known regarding the endophytes of E. prostrata. In this study, we focus on screening and identifying the endophytes from plant E. prostrata and exploring their roles in modulating GM. According to biochemical, physiological tests and 16S rDNA sequence analysis, ten endophytes were characterized in different locations of plant E. prostrata belonging to Lactococcus, Bacillus, Enterococcus, Exiguobacterium and Pantoea. The antimicrobial activity of endophytes EP01-10 was investigated via the oxford cup method. Furthermore, the acid and bile salt resistant ability of EP01-10 was detected to explore their survival ability in gastrointestinal tract. Results indicated that strains of Lactococcus, Bacillus and Exiguobacterium (EP01, 03 and 05) showed strong antimicrobial activity against Escherichia coli and Staphylococcus aureus and strains of Lactococcus, Bacillus and Enterococcus (EP01, 02, 04, 08 and 10) have the ability to survive in the gastrointestinal tract. 16S rRNA sequencing of GM in C57BL/6J mice was performed for diversity and structure analysis responded to the administration of E. prostrata extract. E. prostrata extract acted on improving the microflora diversity, particularly in increasing the abundance of Lactococcus significantly. Thus, as an endophyte, Lactococcus plays an important role in E. prostrata modulating intestinal tract. Our study suggested that endophytes of Chinese herbal medicines might be a novel target for the treatment of diseases by regulating the GM.展开更多
Current drugs against multiple human malignancies are limited,and developing new treatment strategies is improbable within a short time.So,considering the current situation,phytochemicals could be a viable option for ...Current drugs against multiple human malignancies are limited,and developing new treatment strategies is improbable within a short time.So,considering the current situation,phytochemicals could be a viable option for developing chemotherapeutic agents for managing numerous types of malignancies.Therefore,we aimed to locate all phytochemicals of Eclipta prostrata and unravel their chemotherapeutic mechanisms and strategies individually.We defined 25 active compounds based on their anticancer activities after filtering through IMPPAT and literature search,and these compounds have chemotherapeutic activities against around 15 different human malignancies.In vitro and preclinical models support that the chemotherapeutic properties of phytochemicals derived from Eclipta prostrata are believed to be regulated by many pathways,including targeting signaling pathways,for example,phosphoinositide 3-kinase(PI3K)/AKT/mammalian target of rapamycin(mTOR),tumor necrosis factor-alpha(TNF-α),nuclear Factor-kappa B(NF-κB),mitogen-activated protein kinase(MAPK);regulated cell death such as Fas cell surface death receptor(FAS),Bid,apoptosis-inducing factor(AIF),Bcl2,Bax,Bak,Bad,caspase,and Poly(ADPribose)Polymerase(PARP);metastasis and angiogenesis such as matrix metalloproteinases(MMPs)(2&9),wingless/integrated(Wnt)/beta-catenin,angiogenesis(E-cCadherin&N-cadherin,vimentin),cell proliferation(cyclins-A,B1,D1,E1,and cyclindependent kinases(CDKs)1,2,4),inflammatory molecules(programmed death-ligand 1(PD-L1),TNF-α,NF-κB,Interleukin-1(IL-1),Interleukin-6(IL-6),Interleukin-8(IL-8),Interleukin-1 beta(IL-1β)),regulating tumor suppressor genes(p21,p27,p38,p51,p53),microRNA(miRNA)regulation,and some nonspecific pathways like DNA fragmentation damage and repair,autophagy(light chain 3-II(LC3-II)and mTOR),and many other pathways.Some selective phytochemicals exert synergistic activities with standard chemotherapeutic drugs and reverse drug resistance through several mechanisms.Nano-based phytochemicals target numerous cancer cells,resulting in drug accumulation and improved drug efficacy,making phytochemicals more potent chemotherapeutic agents in cancer treatment.Additionally,an in-silico pharmacokinetics study reveals that phytoestrogen possesses suitable pharmacokinetic characteristics with minor toxicity in the human body.So,direct consumption of different parts of Eclipta prostrata or specific phytochemicals from this plant can be a potential candidate drug against human malignancies.展开更多
Abstract: Objective To study the constituents in the dried aerial parts of Eclipta prostrata. Methods The constituents wereisolated and purified by column chromatography and their structures were elucidated by spectro...Abstract: Objective To study the constituents in the dried aerial parts of Eclipta prostrata. Methods The constituents wereisolated and purified by column chromatography and their structures were elucidated by spectroscopic methods (1D,2D NMR, UV, IR, and HRESI-TOF-MS) and chemical analyses. Results Eight compounds were isolated andidentified as 7-O-methylorobol-4′-O-β-D-glucopyranoside (1), 3′-hydroxybiochanin A (2), echinocystic acid28-O-β-D-glucopyranoside (3), ecliptasaponin A (4), eclalbasaponin I (5), eclalbasaponin IV (6), echinocystic acid(7), and 3-oxo-16α-hydroxy-olean-12-en-28-oic acid (8). Conclusion Compound 1 is a new compound andcompound 3 is obtained from this genus for the first time.展开更多
Radiation protection drugs are often accompanied by toxicity,even amifostine,which has been the dominant radio-protecting drug for nearly 30 years.Furthermore,there is no therapeutic drug for radiation-induced intesti...Radiation protection drugs are often accompanied by toxicity,even amifostine,which has been the dominant radio-protecting drug for nearly 30 years.Furthermore,there is no therapeutic drug for radiation-induced intestinal injury(RⅢ).This paper intends to find a safe and effective radioprotecting ingredient from natural sources.The radio-protecting effect of Ecliptae Herba(EHE) was discovered preliminarily by antioxidant experiments and the mouse survival rate after137Cs irradiation.EHE components and blood substances in vivo were identified through UPLC-Q-TOF.The correlation network of “natural components in EHE-constituents migrating to blood-targets-pathways” was established to predict the active components and pathways.The binding force between potential active components and targets was studied by molecular docking,and the mechanism was further analyzed by Western blotting,cellular thermal shift assay(CETSA),and Ch IP.Additionally,the expression levels of Lgr5,Axin2,Ki67,lysozyme,caspase-3,caspase-8,8-OHd G,and p53 in the small intestine of mice were detected.It was found for the first time that EHE is active in radiation protection and that luteolin is the material basis of this protection.Luteolin is a promising candidate for RⅢ.Luteolin can inhibit the p53 signaling pathway and regulate the BAX/BCL2 ratio in the process of apoptosis.Luteolin could also regulate the expression of multitarget proteins related to the same cell cycle.展开更多
Type 2 diabetes mellitus(T2DM)is prevalent in the world while research on its pathogenesis and treatment is still unsatisfactory.Active ingredients of traditional Chinese medicines including Eclipta prostrata(E.Prostr...Type 2 diabetes mellitus(T2DM)is prevalent in the world while research on its pathogenesis and treatment is still unsatisfactory.Active ingredients of traditional Chinese medicines including Eclipta prostrata(E.Prostrata)have been gradually applied to cure various diseases and many in vitro studies have revealed that E.Prostrata has a good hypoglycemic effect.The main objective of this research was to investigate the isolation of E.Prostrata flavonoids(EPFs)and saponins(EPSs),chemical constituents and their hypoglycemic effect on IR-HepG2 cells as well as the potential mechanisms.We determined of the Chemical Constituent was using UPLC-HESI/MS/MS to clarify its chemical substance basis;We established of insulin resistant HepG2 cell line(IR-HepG2)model to evaluate on insulin resistance;We examined Glucose and lipid metabolism,regulate antioxidant system,mitochondrial function as well as APN/AMPK and IRS-1/PI3K/AKT signaling pathway in IR-HepG2 cells treated with EPFs and EPSs to further explore the mechanisms of EPFs and EPSs hypoglycemic activity.The results showed that E.Prostrata extractions treatment could promote glucose uptake and glycogen synthesis,reduce lipid accumulation,improve mitochondrial function and reduce ROS production in IR-HepG2 cells.In particular,both EPFs and EPSs perform preferable activation in glucose consumption than metformin hydrochloride(MET).Further results suggest that EPFs and EPSs may play a role in lowering blood glucose through APN/AMPK and IRS-1/PI3K/AKT signaling pathways.These results suggested that E.Prostrata extractions ideal hypoglycemic activity and worthy of further study.展开更多
为筛选大豆田中对常见杂草鳢肠和马瓟瓜防除效果好的除草剂,通过整株生物测定法测定24种常用除草剂(主要包括乙酰乳酸合成酶ALS类、原卟啉原氧化酶PPO抑制类、光合作用PSⅡ抑制类除草剂)茎叶喷雾处理对鳢肠和马瓟瓜的除草活性及对大豆...为筛选大豆田中对常见杂草鳢肠和马瓟瓜防除效果好的除草剂,通过整株生物测定法测定24种常用除草剂(主要包括乙酰乳酸合成酶ALS类、原卟啉原氧化酶PPO抑制类、光合作用PSⅡ抑制类除草剂)茎叶喷雾处理对鳢肠和马瓟瓜的除草活性及对大豆的安全性。结果表明:ALS抑制类除草剂在药后3、7 d对鳢肠和马瓟瓜的防除效果不佳,药后14 d有所提升,其中噻吩磺隆对2种杂草的鲜重抑制率达88.31%~94.61%。PPO抑制类除草剂苯嘧磺草胺、氟磺胺草醚、乙氧氟草醚和PSⅡ抑制类除草剂灭草松、辛酰溴苯腈、氨唑草酮处理后对鳢肠的防除效果在药后3、7、14 d整体均表现为良好,其中70%苯嘧磺草胺水分散粒剂(WDG)26.25~78.75 g a.i./hm^(2)、480 g/L灭草松水剂(AS)540~1440 g a.i./hm^(2)、30%辛酰溴苯腈乳油(EC)168.75~405 g a.i./hm^(2)处理的鲜重抑制率均达100.00%,防除效果理想。PPO抑制类除草剂28%三氟羧草醚微乳剂(ME)225~450 g a.i./hm^(2)、10%乙羧氟草醚EC 60~90 g a.i./hm^(2)、250 g/L氟磺胺草醚AS 225~450 g a.i./hm^(2)处理在施用后7、14 d,其对马瓟瓜的株数抑制率和鲜重抑制率达86.50%~100.00%;PSⅡ抑制类除草剂38%莠去津悬浮剂(SC)225~450 g a.i./hm^(2)和40%氰草津SC 150~300 g a.i./hm^(2)处理虽在药后3 d对马瓟瓜的防除效果较差,但药后7、14 d的株抑制率和鲜重抑制率达94.58%~100.00%。安全性试验结果表明,PPO抑制类苯嘧磺草胺、三氟羧草醚、乙羧氟草醚、氟磺胺草醚和PSⅡ抑制类除草剂灭草松、辛酰溴苯腈对大豆安全,但莠去津和氰草津对大豆会产生严重的药害。综上所述,PPO抑制类苯嘧磺草胺和PSⅡ抑制类除草剂灭草松、辛酰溴苯腈可以作为防除大豆田鳢肠的候选药剂,PPO抑制类三氟羧草醚、乙羧氟草醚、氟磺胺草醚可以作为马瓟瓜的候选防除药剂。展开更多
文摘Two new triterpenoid glucosides ecliptasaponin A(3)and ecliptasaponin B(4)were isolated together with echinocystic acid(1)and oleanolic acid(2)from Eclipta alba(L.) Hassk.Their structures were deduced as 3β,16a-dihydroxy olean-12-ene-28-oic acid-3β-O-β-D-glu-copyranoside(3)and 3β-O-[β-D-glucopyranosyl(1-4)]-β-D-glucopyranosyl-16a-hydroxy olean-12-ene-28-oic acid 28-O-β-D-glucopyranoside(4),based on spectral analysis and chemical evidences as well as results ofhydrolysis.
文摘Five triterpenoid saponins were isolated from the Chinese traditional medicine Eclipta prostrata L.. On the basis of their chemical properties and spectral data, they were identified as eclalbasaponins II(1), I(2), III(3), 3-O-[b-D-glucopyranosyl(12)-b-D-glucopyranosyl]-16a-ethoxy-olean-12-ene-28-oic acid-28-O-b-D-glu-copyranoside(4) and 3-O-[(2-O-sulfuryl-b-D-glucopyranosyl)(12)-b-D-glucopyranosyl]-echinocystic acid-28-O-b-D-glucopyranoside(5). Compounds 4 and 5 are new compounds and named eclalbasaponins XI and XII, respectively. Compounds 1 and 5 induced morphological deformation of Pyricularia oryzae mycelia.
基金the Department of Science and Technology(DST),New Delhi,India for providing financial assistance for the present investigation
文摘Objective:The present study deals with the investigation of larvicidal and ovicidal activities of benzene,hexane,ethyl acetate,methanol and chloroform leaf extract of Eclipta alba（E.alba） against dengue vector,Aedes aegypti（Ae.Aegypti）.Methods:Twenty five earlyⅢinstar larvae of Ae.aegypti was exposed to various concentrations（50-300 ppm） and was assayed in the laboratory by using the protocol of WHO 2005;the 24 h LC<sub>50</sub> values of the E.alba leaf extract was determined by Probit analysis.For ovicidal activity,slightly modified method of Su and Mulla was performed.The ovicidal activity was determined against Ae.aegypti to various concentrations ranging from 100-350 ppm under the laboratory conditions.The egg hatch rates were assessed 48 h post treatment.Results:The LC<sub>50</sub> values of benzene,hexane,ethyl acetate,methanol and chloroform extract of E.alba against early third instar larvae of Ae.aegypti were 151.38,165.10, 154.88,127.64 and 146.28 ppm,respectively.Maximum larvicidal activity was observed in the methanol extract followed by chloroform,benzene,ethyl acetate and hexane extract.No mortality was observed in control.Among five solvent tested the methanol extract was found to be most effective for ovicidal activity against Ae.aegypti.The methanol extracts exerted 100%mortality （zero hatchability） at 300 ppm.Conclusions:From the results it can be concluded the crude extract of E.alba was an excellent potential for controlling Ae.aegypti mosquito.
基金Supported by the by the Natural Science Foundation of Henan:Study of Eclipta Prostrata Extract in Aging(2020-ZZJH-339,17A180010)
文摘OBJECTIVE: To investigate the role of Eclipta prostrata (E. prostrata) extract in improving spatial learning and memory deficits in D-galactose-induced aging in rats. METHODS: Rats were divided into five groups, with 10 animals in each group. Aging rats were produced by treatment with 100 mg·kg-1·d-1 of D-galactose for 6 weeks. Rats in the E. prostrata treatment groups received an aqueous extract of E. prostrata orally at a concentration of 50, 100, or 200 mg·kg-1· d-1 for 3 weeks. Animals in both the normal and model groups were treated with similar volumes of saline. Spatial memory performance was measured using the Morris water maze. The mRNA levels and enzyme activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and glutathione reductase (GR) were analyzed using real- time quantitative PCR and spectrophotometry,respectively. The levels of induced nitric oxide synthase (iNOS), nitric oxide (NO), dopamine (DA), norepinephrine (NE), and serotonin (5-HT) were determined using enzyme-linked immunosorbent assay and spectrophotometry. RESULTS: Compared with the normal group, rats in the D-galactose-treated model group exhibited significant memory loss. There was severe damage to the hippocampal CA1 area, and expression levels of SOD, CAT, GPx, and GR were significantly decreased in the model group compared with the normal group. In the model group, levels of iNOS and NO were significantly increased compared with the normal group. However, treatment with E. prostrata extract reversed the conditions caused by D-galactose- induced aging, especially in the groups with higher treatment concentrations. Compared with the normal group, the levels of DA, NE, and 5-HT were significantly lower in the D-galactose-treated model group. In the E. prostrata extract-treated groups, however, there was a dose-dependent upregulation of DA, NE, and 5-HT expression. CONCLUSION: Our results suggest that administration of E. prostrata extract can result in an improvement in the learning and memory impairments that are induced by D-galactose treatment in rats. This improvement may be the result of enhanced antioxidative ability, decreased iNOS and NO levels, and the induction of DA, NE, and 5-HT expression in the brain.
文摘Due to ease of application, cost effectiveness and environmentally safe, in this study, the corrosion inhibition effect of aqueous extract of Eclipta alba in 1N hydrochloric acid has been investigated by weight loss, potentiodynamic polarization and impedance methods and the extracts of Eclipta alba were found to be effective corrosion pickling inhibitor. The effect of immersion time and temperature revealed that the extracts of Eclipta alba with an optimum concentration of 8.0% v/v showed maximum inhibition efficiency of 99.6% at 3 h immersion time and 30℃. Arrhenius plots for mild steel immersed in 1N HCl solution in the absence and presence of optimum concentration （8.0% in v/v） of Eclipta alba extract showed the effect of temperature. Polarization studies indicate that this plant extract acts as a mixed type inhibitor. The adsorption of Eclipta alba follows Langmuir adsorption isotherm. The inhibition action may be due to the presence of the Wedelactone and also the alkaloid Ecliptine present in the leaves of Eclipta alba.
基金support from the Fund of The Key Laboratory of Enzyme and Protein Technology,VNU University of Science(KLEPT:22.02).
文摘Objective:To evaluate the effects of wedelolactone,a major flavonoid from Vietnamese Eclipta prostrata(L)L.,on inflammation and insulin resistance.Methods:Wedelolactone was extracted from the leaves of Vietnamese Eclipta prostrata(L.)L.with methanol by Soxhlet.The effects of wedelolactone on lipopolysaccharide(LPS)-induced cytokine production,reactive oxygen species(ROS)generation,and nicotinamide adenine dinucleotide phosphate(NADPH)oxidase activities in Raw 264.7 cells were measured by enzyme-linked immunosorbent assay(ELISA),specific immunofluorescent dyes and luminometric analysis,respectively.In addition,its effects on glucose uptake and the protein expression of insulin receptor substrate 1(IRS1)and glucose transporter 4(GLUT4)were examined in 3T3-L1 cells by immunofluorescent dyes and Western blot.Results:Wedelolactone at 30μg/mL significantly inhibited LPS-induced production of tumor necrosis factor-α,interleukin(IL)-6,and IL-8(P<0.01)with no noticeable effects on IL-10 level.It also reduced ROS generation and NADPH oxidase activities in LPS-stimulated Raw 264.7 cells(P<0.01).Furthermore,wedelolactone showed anti-insulin resistance activity,as evidenced by improved glucose uptake and the upregulated expression of IRS1 and GLUT4 in 3T3-L1 cells(P<0.01).Conclusions:Wedelolactone exhibits anti-inflammation and anti-insulin resistance effects,which may be used for the treatment of diabetes and inflammation-associated diseases.
文摘Eclipta prostrata has been extensively studied for its promoting effect on human health. Previous study proved that E. prostrata possessed anti-osteoporosis function in SAMP6 mice via gut microbiota (GM). Endophytes are widespread in plants, especially in Chinese herbal medicines. However, little is known regarding the endophytes of E. prostrata. In this study, we focus on screening and identifying the endophytes from plant E. prostrata and exploring their roles in modulating GM. According to biochemical, physiological tests and 16S rDNA sequence analysis, ten endophytes were characterized in different locations of plant E. prostrata belonging to Lactococcus, Bacillus, Enterococcus, Exiguobacterium and Pantoea. The antimicrobial activity of endophytes EP01-10 was investigated via the oxford cup method. Furthermore, the acid and bile salt resistant ability of EP01-10 was detected to explore their survival ability in gastrointestinal tract. Results indicated that strains of Lactococcus, Bacillus and Exiguobacterium (EP01, 03 and 05) showed strong antimicrobial activity against Escherichia coli and Staphylococcus aureus and strains of Lactococcus, Bacillus and Enterococcus (EP01, 02, 04, 08 and 10) have the ability to survive in the gastrointestinal tract. 16S rRNA sequencing of GM in C57BL/6J mice was performed for diversity and structure analysis responded to the administration of E. prostrata extract. E. prostrata extract acted on improving the microflora diversity, particularly in increasing the abundance of Lactococcus significantly. Thus, as an endophyte, Lactococcus plays an important role in E. prostrata modulating intestinal tract. Our study suggested that endophytes of Chinese herbal medicines might be a novel target for the treatment of diseases by regulating the GM.
文摘Current drugs against multiple human malignancies are limited,and developing new treatment strategies is improbable within a short time.So,considering the current situation,phytochemicals could be a viable option for developing chemotherapeutic agents for managing numerous types of malignancies.Therefore,we aimed to locate all phytochemicals of Eclipta prostrata and unravel their chemotherapeutic mechanisms and strategies individually.We defined 25 active compounds based on their anticancer activities after filtering through IMPPAT and literature search,and these compounds have chemotherapeutic activities against around 15 different human malignancies.In vitro and preclinical models support that the chemotherapeutic properties of phytochemicals derived from Eclipta prostrata are believed to be regulated by many pathways,including targeting signaling pathways,for example,phosphoinositide 3-kinase(PI3K)/AKT/mammalian target of rapamycin(mTOR),tumor necrosis factor-alpha(TNF-α),nuclear Factor-kappa B(NF-κB),mitogen-activated protein kinase(MAPK);regulated cell death such as Fas cell surface death receptor(FAS),Bid,apoptosis-inducing factor(AIF),Bcl2,Bax,Bak,Bad,caspase,and Poly(ADPribose)Polymerase(PARP);metastasis and angiogenesis such as matrix metalloproteinases(MMPs)(2&9),wingless/integrated(Wnt)/beta-catenin,angiogenesis(E-cCadherin&N-cadherin,vimentin),cell proliferation(cyclins-A,B1,D1,E1,and cyclindependent kinases(CDKs)1,2,4),inflammatory molecules(programmed death-ligand 1(PD-L1),TNF-α,NF-κB,Interleukin-1(IL-1),Interleukin-6(IL-6),Interleukin-8(IL-8),Interleukin-1 beta(IL-1β)),regulating tumor suppressor genes(p21,p27,p38,p51,p53),microRNA(miRNA)regulation,and some nonspecific pathways like DNA fragmentation damage and repair,autophagy(light chain 3-II(LC3-II)and mTOR),and many other pathways.Some selective phytochemicals exert synergistic activities with standard chemotherapeutic drugs and reverse drug resistance through several mechanisms.Nano-based phytochemicals target numerous cancer cells,resulting in drug accumulation and improved drug efficacy,making phytochemicals more potent chemotherapeutic agents in cancer treatment.Additionally,an in-silico pharmacokinetics study reveals that phytoestrogen possesses suitable pharmacokinetic characteristics with minor toxicity in the human body.So,direct consumption of different parts of Eclipta prostrata or specific phytochemicals from this plant can be a potential candidate drug against human malignancies.
基金Program for New Century Excellent Talents in University(NCET-10-0958)Important Drug Development Fund,Ministry of Science and Technology of China(2011ZX09307-002-01)
文摘Abstract: Objective To study the constituents in the dried aerial parts of Eclipta prostrata. Methods The constituents wereisolated and purified by column chromatography and their structures were elucidated by spectroscopic methods (1D,2D NMR, UV, IR, and HRESI-TOF-MS) and chemical analyses. Results Eight compounds were isolated andidentified as 7-O-methylorobol-4′-O-β-D-glucopyranoside (1), 3′-hydroxybiochanin A (2), echinocystic acid28-O-β-D-glucopyranoside (3), ecliptasaponin A (4), eclalbasaponin I (5), eclalbasaponin IV (6), echinocystic acid(7), and 3-oxo-16α-hydroxy-olean-12-en-28-oic acid (8). Conclusion Compound 1 is a new compound andcompound 3 is obtained from this genus for the first time.
基金supported by the Chinese Academy of Medical Sciences Innovation Fund for Medical Sciences(2021-I2M-1-042,China).
文摘Radiation protection drugs are often accompanied by toxicity,even amifostine,which has been the dominant radio-protecting drug for nearly 30 years.Furthermore,there is no therapeutic drug for radiation-induced intestinal injury(RⅢ).This paper intends to find a safe and effective radioprotecting ingredient from natural sources.The radio-protecting effect of Ecliptae Herba(EHE) was discovered preliminarily by antioxidant experiments and the mouse survival rate after137Cs irradiation.EHE components and blood substances in vivo were identified through UPLC-Q-TOF.The correlation network of “natural components in EHE-constituents migrating to blood-targets-pathways” was established to predict the active components and pathways.The binding force between potential active components and targets was studied by molecular docking,and the mechanism was further analyzed by Western blotting,cellular thermal shift assay(CETSA),and Ch IP.Additionally,the expression levels of Lgr5,Axin2,Ki67,lysozyme,caspase-3,caspase-8,8-OHd G,and p53 in the small intestine of mice were detected.It was found for the first time that EHE is active in radiation protection and that luteolin is the material basis of this protection.Luteolin is a promising candidate for RⅢ.Luteolin can inhibit the p53 signaling pathway and regulate the BAX/BCL2 ratio in the process of apoptosis.Luteolin could also regulate the expression of multitarget proteins related to the same cell cycle.
基金supported by the National Natural Science Foundation of China(32072201and 32372286)Guangdong Provincial Natural Science Foundation Project,China(2023A1515011967 and 2023A1515012223)。
文摘Type 2 diabetes mellitus(T2DM)is prevalent in the world while research on its pathogenesis and treatment is still unsatisfactory.Active ingredients of traditional Chinese medicines including Eclipta prostrata(E.Prostrata)have been gradually applied to cure various diseases and many in vitro studies have revealed that E.Prostrata has a good hypoglycemic effect.The main objective of this research was to investigate the isolation of E.Prostrata flavonoids(EPFs)and saponins(EPSs),chemical constituents and their hypoglycemic effect on IR-HepG2 cells as well as the potential mechanisms.We determined of the Chemical Constituent was using UPLC-HESI/MS/MS to clarify its chemical substance basis;We established of insulin resistant HepG2 cell line(IR-HepG2)model to evaluate on insulin resistance;We examined Glucose and lipid metabolism,regulate antioxidant system,mitochondrial function as well as APN/AMPK and IRS-1/PI3K/AKT signaling pathway in IR-HepG2 cells treated with EPFs and EPSs to further explore the mechanisms of EPFs and EPSs hypoglycemic activity.The results showed that E.Prostrata extractions treatment could promote glucose uptake and glycogen synthesis,reduce lipid accumulation,improve mitochondrial function and reduce ROS production in IR-HepG2 cells.In particular,both EPFs and EPSs perform preferable activation in glucose consumption than metformin hydrochloride(MET).Further results suggest that EPFs and EPSs may play a role in lowering blood glucose through APN/AMPK and IRS-1/PI3K/AKT signaling pathways.These results suggested that E.Prostrata extractions ideal hypoglycemic activity and worthy of further study.
文摘为筛选大豆田中对常见杂草鳢肠和马瓟瓜防除效果好的除草剂,通过整株生物测定法测定24种常用除草剂(主要包括乙酰乳酸合成酶ALS类、原卟啉原氧化酶PPO抑制类、光合作用PSⅡ抑制类除草剂)茎叶喷雾处理对鳢肠和马瓟瓜的除草活性及对大豆的安全性。结果表明:ALS抑制类除草剂在药后3、7 d对鳢肠和马瓟瓜的防除效果不佳,药后14 d有所提升,其中噻吩磺隆对2种杂草的鲜重抑制率达88.31%~94.61%。PPO抑制类除草剂苯嘧磺草胺、氟磺胺草醚、乙氧氟草醚和PSⅡ抑制类除草剂灭草松、辛酰溴苯腈、氨唑草酮处理后对鳢肠的防除效果在药后3、7、14 d整体均表现为良好,其中70%苯嘧磺草胺水分散粒剂(WDG)26.25~78.75 g a.i./hm^(2)、480 g/L灭草松水剂(AS)540~1440 g a.i./hm^(2)、30%辛酰溴苯腈乳油(EC)168.75~405 g a.i./hm^(2)处理的鲜重抑制率均达100.00%,防除效果理想。PPO抑制类除草剂28%三氟羧草醚微乳剂(ME)225~450 g a.i./hm^(2)、10%乙羧氟草醚EC 60~90 g a.i./hm^(2)、250 g/L氟磺胺草醚AS 225~450 g a.i./hm^(2)处理在施用后7、14 d,其对马瓟瓜的株数抑制率和鲜重抑制率达86.50%~100.00%;PSⅡ抑制类除草剂38%莠去津悬浮剂(SC)225~450 g a.i./hm^(2)和40%氰草津SC 150~300 g a.i./hm^(2)处理虽在药后3 d对马瓟瓜的防除效果较差,但药后7、14 d的株抑制率和鲜重抑制率达94.58%~100.00%。安全性试验结果表明,PPO抑制类苯嘧磺草胺、三氟羧草醚、乙羧氟草醚、氟磺胺草醚和PSⅡ抑制类除草剂灭草松、辛酰溴苯腈对大豆安全,但莠去津和氰草津对大豆会产生严重的药害。综上所述,PPO抑制类苯嘧磺草胺和PSⅡ抑制类除草剂灭草松、辛酰溴苯腈可以作为防除大豆田鳢肠的候选药剂,PPO抑制类三氟羧草醚、乙羧氟草醚、氟磺胺草醚可以作为马瓟瓜的候选防除药剂。
