The effect of La 3+ and Eu 3+ on the rooting of Eriobotrya japonica Lindl. plantlet in vitro was studied with adding La 3+ and Eu 3+ to the rooted medium. The rooting rate, the number of root and the lengt...The effect of La 3+ and Eu 3+ on the rooting of Eriobotrya japonica Lindl. plantlet in vitro was studied with adding La 3+ and Eu 3+ to the rooted medium. The rooting rate, the number of root and the length of root were studied after transplanting 20 d. The activity of peroxidase, nitrate reductase and fresh weight of roots were determined after transplanting 44 d. The results show that the optimum concentration range of La 3+ (1.0～3.0 μmol·L -1), Eu 3+ (2.0～3.0 μmol·L -1) in the rooted medium can increase the rooting rate and the fresh weight of roots, and promote the length of root and raise the activities of peroxidase and nitrate reductase significantly. La 3+ has more effect in improving the rooting rate, root length and the activities of peroxidase and less effect in promoting root fresh weight and the activities of nitrate reductase than Eu 3+.展开更多
The purpose of this study was to investigate the effects of various presowing treatments on the germinability(final germination percentage)and germination rate of loquat seeds in order to increase seedling production ...The purpose of this study was to investigate the effects of various presowing treatments on the germinability(final germination percentage)and germination rate of loquat seeds in order to increase seedling production in nurseries(applied research)as well as provide answers for important physiological issues related to loquat seeds and their seed coat(basic research).Three experiments were carried out with various pre-sowing treatments.These treatments included full or partial removal of seed coat(perisperm),partial cutting of cotyledons as well as moist chilling at 5℃ for 13 days and/or soaking the seeds in water or 250 ppm gibberellic acid(GA_(3))solution for 24 h.According to the results,cotyledons excision resulted in delayed germination,regardless of the presence or absence of the seed coat in comparison with the decoated seeds that demonstrated the highest germination rate amongst them.In addition,even the partial excision of seed coats affected positively both the germinability and the germination rate,compared to the control-intact seeds.Furthermore,control-intact seeds had a higher germination percentage when exposed to moist chilling independently of the application or not of gibberellin;while the combination of gibberellin application and moist chilling improved both the percentage and the rate of germination of decoated seeds.In conclusion,the role of perisperm(seed coat)in the germination procedure of loquat seeds seems to be important,indicating the existence of seed coat-imposed dormancy on loquat seeds.Finally,the existence of a mild endogenous embryo-dormancy on loquat is also discussed.展开更多
The objective of this research was investigated the effect of polyphenol oxidase microwave treatment on phenolic composition, antioxidant activity and microstructure of loquat fruit. Phenolic profile of methanolic ext...The objective of this research was investigated the effect of polyphenol oxidase microwave treatment on phenolic composition, antioxidant activity and microstructure of loquat fruit. Phenolic profile of methanolic extracts prepared from fresh, and microwave-treated samples were analyzed. Antioxidant activity was also evaluated by 2,2’-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS?+) and 1,1-diphenyl-2-picrylhydrazyl (DPPH+) methods. In addition, polyphenol oxidase inactivation was carried out using a response surface methodology to establish the optimal conditions of treatment. The phenolic content of fresh mesocarp was 311 ± 0.60 mg gallic acid equivalents (GAE)/100g dry weight (DW) and that of microwave-treated mesocarp was 1230 ± 0.36 mg GAE/100g DW. Total phenolic content of water/ methanol extract significantly increases after microwave treatment rather than methanolic extract of fresh loquat. Five glycoside phenolics were identified by HPLC-DAD-MS as 3-caffeoylquinic acid, 3-p-coumaroylquinic acid, 5-caffeoylquinic acid and quercetin-3-O-sambubioside. Methanolic extract of microwave-treated mesocarp showed higher antioxidant activity than that of fresh mesocarp. Thus, polyphenol oxidase inactivation by microwave energy preserved the integrity of phenolic compounds as well as antioxidant activity in mesocarp extracts prepared from loquat fruit. It was also noted that phenolics were more abundant in the microwaved samples than in the fresh samples.展开更多
A devastating trunk beetle has been found in Baisha loquat in Dongshan Town, Wuzhong District, Suzhou City in recent years. It is iden-tified as a new insect species, Cryphalus eriobotryae, belonging to Scolytinae, Cu...A devastating trunk beetle has been found in Baisha loquat in Dongshan Town, Wuzhong District, Suzhou City in recent years. It is iden-tified as a new insect species, Cryphalus eriobotryae, belonging to Scolytinae, Curculionidae, Coleoptera. So far, loquat is known as the unique host of C. eriobotryae, and trees of 20-30 years old or 3-4 years old are all attacked by the insect. Holes drilled by adults with the diameter of about0.8 mm and sores(ate by adults) with the diameter of 1 mm are found on bark and trunk of damaged trees. Three-dimensional channels in 3-4 layers are formed in cortex, which are filled with insect dung. The phloem turns black-brown in late stage, and loses transport function, resulting in plant death and a great impact on loquat production and industrial development. During the occurrence period of C. eriobotryae from April to November,the damaged trunks and branches should be sprayed with 48% chlorpyrifos EC or 5% imidacloprid EC 100 times dilution once every 10 d for con-tinuous 2-3 times, and various states of the insect could be killed. If the phloem is damaged in the early and middle stages and does not turn black and brown completely, the plant could grow new cortex to realize recovery.展开更多
Loquat(Eriobotrya japonica Lindl.)is a subtropical evergreen fruit tree that produces fruits with abundant nutrients and medicinal components.Confirming suitable reference genes for a set of loquat samples before qRT-...Loquat(Eriobotrya japonica Lindl.)is a subtropical evergreen fruit tree that produces fruits with abundant nutrients and medicinal components.Confirming suitable reference genes for a set of loquat samples before qRT-PCR experiments is essential for the accurate quantification of gene expression.In this study,eight candidate reference genes were selected from our previously published RNA-seq data,and primers for each candidate reference gene were designed and evaluated.The Cq values of the candidate reference genes were calculated by RT-qPCR in 31 different loquat samples,including 12 subgroups of developing or abiotic-stressed tissues.Different combinations of stable reference genes were screened according to a comprehensive rank,which was synthesized from the results of four algorithms,including the geNorm,NormFinder,BestKeeper andΔCt methods.The screened reference genes were verified by normalizing EjLGA1 in each subgroup.The obtained suitable combinations of reference genes for accurate normalization were GAPDH,EF1αand ACT for floral development;GAPDH,UBCE and ACT for fruit setting;EF1α,GAPDH and eIF2B for fruit ripening;ACT,EF1αand UBCE for leaves under heat stress;eIF2B,UBCE and EF1αfor leaves under freezing stress;EF1α,TUA and UBCE for leaves under salt stress;ACT,EF1αand eIF2B for immature pulp under freezing stress;ACT,UBCE and eIF2B for immature seeds under freezing stress;EF1α,eIF2B and UBCE for both immature pulp and seeds under freezing stress;UBCE,TUB and TUA for red-fleshed fruits under cold-storage stress;eIF2B,RPS3 and TUB for white-fleshed fruits under coldstorage stress;and eIF2B,UBCE and RPS3 for both red-and white-fleshed fruits under cold-storage stress.This study obtained different combinations of stable reference genes for accurate normalization in twelve subgroups of developing or abiotic-stressed tissues in loquat.To our knowledge,this is the first report to obtain stable reference genes for normalizing gene expression of abiotic-stressed tissues in E.japonica.The use of the three most stable reference genes could increase the reliability of future quantification experiments.展开更多
We studied the cross-compatibility among 91 inter-specific combinations and 21 inter-generic combinations in 7 Eriobotrya plants and 2 related genera(Raphiolepis indica Lindl. and Photinia serrulata Lindl.) using emas...We studied the cross-compatibility among 91 inter-specific combinations and 21 inter-generic combinations in 7 Eriobotrya plants and 2 related genera(Raphiolepis indica Lindl. and Photinia serrulata Lindl.) using emasculation, bagging, and artificial pollination. Our results showed that28 of the 91 inter-specific combinations set no fruit, which means nearly 30% of the combinations were incompatible. In the remaining 63 combinations, most showed partial cross-compatibility, and a few showed complete cross compatibility. Eriobotrya plants were incompatible with plants from their related genera(R. indica Lindl. and P. serrulata Lindl.). Backcrossing produced 5 compatible combinations, which could set fruits and produce F1 progeny but only after embryo rescue. Fruit setting ratios varied among various species used as male or female parents.E. prinoides Rehd. & Wils., common loquat(E. japonica) and Eriobotrya × daduheensis, used as female parents resulted in an average fruitsetting ratio of 36.2%–58.2%. E. deflexa Nakai and its two forms, and E. elliptica Lindl. as female parents resulted in 2.9%–16.3% average fruitsetting ratio; however, the fruit set ratio was higher(22.4%–43.1%) if they were used as male parents. Failure of E. deflexa f. koshunensis Nakai × E.prinoides Rehd. & Wils. hybrids to set fruit could be attributed to sporophytic incompatibility.展开更多
The genetic relationships among oakleaf loquat(Eriobotrya prinoides),Daduhe loquat(Eriobotrya prinoides var.dadunensis)and common loquat(Eriobotrya japonica)were studied by RAPD and ISSR markers.The results showed tha...The genetic relationships among oakleaf loquat(Eriobotrya prinoides),Daduhe loquat(Eriobotrya prinoides var.dadunensis)and common loquat(Eriobotrya japonica)were studied by RAPD and ISSR markers.The results showed that the similarity coefficient between oakleaf loquat and common loquat was minimal(0.6996 and 0.7219,respectively)and the similarity coefficient between oakleaf loquat and Daduhe loquat was maximal(0.8403 and 0.8211,respectively),while the similarity coefficient between Daduhe loquat and common loquat was always intermediate(0.7195 and 0.7219,respectively).The highest additivity was obtained when Daduhe loquat was regarded as the undetermined hybrid(44.9%and 45.8%,respectively).The specific bands of oakleaf loquat and common loquat were present in Daduhe loquat.Thus,it could be concluded that Daduhe loquat was a hybrid of oakleaf loquat and common loquat.展开更多
[Objective] The paper was to evaluate effects of peracetic acid （PAA） combined with calcium treatments on storage quality of Ioquat fruits, so as to pro- vide practical techniques to solve the problems of postharves...[Objective] The paper was to evaluate effects of peracetic acid （PAA） combined with calcium treatments on storage quality of Ioquat fruits, so as to pro- vide practical techniques to solve the problems of postharvest rot and quality deteri- oration for Ioquat fruits. [Method] With Ioquat fruits of Qingzhong variety as materi- als, 0.2%, 0.4% and 0.8% PAA combined with 0.8% CaCI2 was used to soak Ioquat fruits for 4 min, 0.8% CaCl2 and water treatments were set as two controls; the fruits were dried and packaged by 0.02 mm PE bags, then stored under non-chilling low temperature of （7±1） ℃. The indicators related to storage quality of Ioquat fruits were randomly tested once every 3 d, and their variation situations were analyzed. [Result] Compared with two control treatments and 0.2% PAA, 0.8% PAA combined with calcium treatments, the treatment of 0.4% PAA combined with 0.8% CaCl2 could significantly inhibit rot index, weight loss rate, firmness and cell membrane permeability of Ioquat fruits during storage period, which could also effectively delay the reduction of titratable acid, vitamin C, soluble solid content and juice yield, and maintain respiration intensity of fruits at a low level; the appearance and flavor qual- ity of fruits were good after stored for 25 d. [Conclusion] 0.4% PAA combined with 0.8% CaCl2 treatment is an efficient, safe and economical practice technology in an- ti-corrosion and quality preservation for postharvest Ioquat fruits .展开更多
文摘The effect of La 3+ and Eu 3+ on the rooting of Eriobotrya japonica Lindl. plantlet in vitro was studied with adding La 3+ and Eu 3+ to the rooted medium. The rooting rate, the number of root and the length of root were studied after transplanting 20 d. The activity of peroxidase, nitrate reductase and fresh weight of roots were determined after transplanting 44 d. The results show that the optimum concentration range of La 3+ (1.0～3.0 μmol·L -1), Eu 3+ (2.0～3.0 μmol·L -1) in the rooted medium can increase the rooting rate and the fresh weight of roots, and promote the length of root and raise the activities of peroxidase and nitrate reductase significantly. La 3+ has more effect in improving the rooting rate, root length and the activities of peroxidase and less effect in promoting root fresh weight and the activities of nitrate reductase than Eu 3+.
文摘The purpose of this study was to investigate the effects of various presowing treatments on the germinability(final germination percentage)and germination rate of loquat seeds in order to increase seedling production in nurseries(applied research)as well as provide answers for important physiological issues related to loquat seeds and their seed coat(basic research).Three experiments were carried out with various pre-sowing treatments.These treatments included full or partial removal of seed coat(perisperm),partial cutting of cotyledons as well as moist chilling at 5℃ for 13 days and/or soaking the seeds in water or 250 ppm gibberellic acid(GA_(3))solution for 24 h.According to the results,cotyledons excision resulted in delayed germination,regardless of the presence or absence of the seed coat in comparison with the decoated seeds that demonstrated the highest germination rate amongst them.In addition,even the partial excision of seed coats affected positively both the germinability and the germination rate,compared to the control-intact seeds.Furthermore,control-intact seeds had a higher germination percentage when exposed to moist chilling independently of the application or not of gibberellin;while the combination of gibberellin application and moist chilling improved both the percentage and the rate of germination of decoated seeds.In conclusion,the role of perisperm(seed coat)in the germination procedure of loquat seeds seems to be important,indicating the existence of seed coat-imposed dormancy on loquat seeds.Finally,the existence of a mild endogenous embryo-dormancy on loquat is also discussed.
文摘The objective of this research was investigated the effect of polyphenol oxidase microwave treatment on phenolic composition, antioxidant activity and microstructure of loquat fruit. Phenolic profile of methanolic extracts prepared from fresh, and microwave-treated samples were analyzed. Antioxidant activity was also evaluated by 2,2’-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS?+) and 1,1-diphenyl-2-picrylhydrazyl (DPPH+) methods. In addition, polyphenol oxidase inactivation was carried out using a response surface methodology to establish the optimal conditions of treatment. The phenolic content of fresh mesocarp was 311 ± 0.60 mg gallic acid equivalents (GAE)/100g dry weight (DW) and that of microwave-treated mesocarp was 1230 ± 0.36 mg GAE/100g DW. Total phenolic content of water/ methanol extract significantly increases after microwave treatment rather than methanolic extract of fresh loquat. Five glycoside phenolics were identified by HPLC-DAD-MS as 3-caffeoylquinic acid, 3-p-coumaroylquinic acid, 5-caffeoylquinic acid and quercetin-3-O-sambubioside. Methanolic extract of microwave-treated mesocarp showed higher antioxidant activity than that of fresh mesocarp. Thus, polyphenol oxidase inactivation by microwave energy preserved the integrity of phenolic compounds as well as antioxidant activity in mesocarp extracts prepared from loquat fruit. It was also noted that phenolics were more abundant in the microwaved samples than in the fresh samples.
文摘A devastating trunk beetle has been found in Baisha loquat in Dongshan Town, Wuzhong District, Suzhou City in recent years. It is iden-tified as a new insect species, Cryphalus eriobotryae, belonging to Scolytinae, Curculionidae, Coleoptera. So far, loquat is known as the unique host of C. eriobotryae, and trees of 20-30 years old or 3-4 years old are all attacked by the insect. Holes drilled by adults with the diameter of about0.8 mm and sores(ate by adults) with the diameter of 1 mm are found on bark and trunk of damaged trees. Three-dimensional channels in 3-4 layers are formed in cortex, which are filled with insect dung. The phloem turns black-brown in late stage, and loses transport function, resulting in plant death and a great impact on loquat production and industrial development. During the occurrence period of C. eriobotryae from April to November,the damaged trunks and branches should be sprayed with 48% chlorpyrifos EC or 5% imidacloprid EC 100 times dilution once every 10 d for con-tinuous 2-3 times, and various states of the insect could be killed. If the phloem is damaged in the early and middle stages and does not turn black and brown completely, the plant could grow new cortex to realize recovery.
基金funded by the Natural Science Foundation of Fujian Province(2021J05240)Fujian Provincial Science and Technology Project(2021N5014,2022N5006)+1 种基金Research Project of Putian Science and Technology Bureau(2021ZP08,2021ZP09,2021ZP10,2021ZP11)Scientific Research Project of Putian University(2018064).
文摘Loquat(Eriobotrya japonica Lindl.)is a subtropical evergreen fruit tree that produces fruits with abundant nutrients and medicinal components.Confirming suitable reference genes for a set of loquat samples before qRT-PCR experiments is essential for the accurate quantification of gene expression.In this study,eight candidate reference genes were selected from our previously published RNA-seq data,and primers for each candidate reference gene were designed and evaluated.The Cq values of the candidate reference genes were calculated by RT-qPCR in 31 different loquat samples,including 12 subgroups of developing or abiotic-stressed tissues.Different combinations of stable reference genes were screened according to a comprehensive rank,which was synthesized from the results of four algorithms,including the geNorm,NormFinder,BestKeeper andΔCt methods.The screened reference genes were verified by normalizing EjLGA1 in each subgroup.The obtained suitable combinations of reference genes for accurate normalization were GAPDH,EF1αand ACT for floral development;GAPDH,UBCE and ACT for fruit setting;EF1α,GAPDH and eIF2B for fruit ripening;ACT,EF1αand UBCE for leaves under heat stress;eIF2B,UBCE and EF1αfor leaves under freezing stress;EF1α,TUA and UBCE for leaves under salt stress;ACT,EF1αand eIF2B for immature pulp under freezing stress;ACT,UBCE and eIF2B for immature seeds under freezing stress;EF1α,eIF2B and UBCE for both immature pulp and seeds under freezing stress;UBCE,TUB and TUA for red-fleshed fruits under cold-storage stress;eIF2B,RPS3 and TUB for white-fleshed fruits under coldstorage stress;and eIF2B,UBCE and RPS3 for both red-and white-fleshed fruits under cold-storage stress.This study obtained different combinations of stable reference genes for accurate normalization in twelve subgroups of developing or abiotic-stressed tissues in loquat.To our knowledge,this is the first report to obtain stable reference genes for normalizing gene expression of abiotic-stressed tissues in E.japonica.The use of the three most stable reference genes could increase the reliability of future quantification experiments.
基金supported by Guangdong Science and Technology Basic Condition Construction Project (Grant No. 2015A030303015)Guangzhou Science and Technology Innovation Commission (Grant No. 201504010028)
文摘We studied the cross-compatibility among 91 inter-specific combinations and 21 inter-generic combinations in 7 Eriobotrya plants and 2 related genera(Raphiolepis indica Lindl. and Photinia serrulata Lindl.) using emasculation, bagging, and artificial pollination. Our results showed that28 of the 91 inter-specific combinations set no fruit, which means nearly 30% of the combinations were incompatible. In the remaining 63 combinations, most showed partial cross-compatibility, and a few showed complete cross compatibility. Eriobotrya plants were incompatible with plants from their related genera(R. indica Lindl. and P. serrulata Lindl.). Backcrossing produced 5 compatible combinations, which could set fruits and produce F1 progeny but only after embryo rescue. Fruit setting ratios varied among various species used as male or female parents.E. prinoides Rehd. & Wils., common loquat(E. japonica) and Eriobotrya × daduheensis, used as female parents resulted in an average fruitsetting ratio of 36.2%–58.2%. E. deflexa Nakai and its two forms, and E. elliptica Lindl. as female parents resulted in 2.9%–16.3% average fruitsetting ratio; however, the fruit set ratio was higher(22.4%–43.1%) if they were used as male parents. Failure of E. deflexa f. koshunensis Nakai × E.prinoides Rehd. & Wils. hybrids to set fruit could be attributed to sporophytic incompatibility.
基金Chinese Ministry of Agriculture and Sichuan Department of Science and Technology for their financial support to this study.Projects from National Natural Science Research of Education Department(No.2005A029).
文摘The genetic relationships among oakleaf loquat(Eriobotrya prinoides),Daduhe loquat(Eriobotrya prinoides var.dadunensis)and common loquat(Eriobotrya japonica)were studied by RAPD and ISSR markers.The results showed that the similarity coefficient between oakleaf loquat and common loquat was minimal(0.6996 and 0.7219,respectively)and the similarity coefficient between oakleaf loquat and Daduhe loquat was maximal(0.8403 and 0.8211,respectively),while the similarity coefficient between Daduhe loquat and common loquat was always intermediate(0.7195 and 0.7219,respectively).The highest additivity was obtained when Daduhe loquat was regarded as the undetermined hybrid(44.9%and 45.8%,respectively).The specific bands of oakleaf loquat and common loquat were present in Daduhe loquat.Thus,it could be concluded that Daduhe loquat was a hybrid of oakleaf loquat and common loquat.
基金Supported by Special Fund for Agro-scientific Research in the Public Interest "Tec-hnology Research and Experimental Demonstration of Loquat Industry"(201003073)~~
文摘[Objective] The paper was to evaluate effects of peracetic acid （PAA） combined with calcium treatments on storage quality of Ioquat fruits, so as to pro- vide practical techniques to solve the problems of postharvest rot and quality deteri- oration for Ioquat fruits. [Method] With Ioquat fruits of Qingzhong variety as materi- als, 0.2%, 0.4% and 0.8% PAA combined with 0.8% CaCI2 was used to soak Ioquat fruits for 4 min, 0.8% CaCl2 and water treatments were set as two controls; the fruits were dried and packaged by 0.02 mm PE bags, then stored under non-chilling low temperature of （7±1） ℃. The indicators related to storage quality of Ioquat fruits were randomly tested once every 3 d, and their variation situations were analyzed. [Result] Compared with two control treatments and 0.2% PAA, 0.8% PAA combined with calcium treatments, the treatment of 0.4% PAA combined with 0.8% CaCl2 could significantly inhibit rot index, weight loss rate, firmness and cell membrane permeability of Ioquat fruits during storage period, which could also effectively delay the reduction of titratable acid, vitamin C, soluble solid content and juice yield, and maintain respiration intensity of fruits at a low level; the appearance and flavor qual- ity of fruits were good after stored for 25 d. [Conclusion] 0.4% PAA combined with 0.8% CaCl2 treatment is an efficient, safe and economical practice technology in an- ti-corrosion and quality preservation for postharvest Ioquat fruits .
