摘要
【目的】系统分析枇杷(Eriobotrya japonica)WRKY基因家族成员(EjWRKY),为解析其在果皮着色过程中的生物学功能奠定基础。【方法】基于枇杷基因组数据,采用生物信息学方法鉴定枇杷EjWRKY基因家族,并对其理化性质、系统进化和结构特征等进行全面分析。同时,结合转录组测序技术,比较分析‘解放钟’和‘山牌3号’枇杷果皮着色过程中EjWRKY基因的表达情况,并采用实时荧光定量PCR(quantitative real-time polymerase chain reaction,qRT-PCR)技术对部分差异表达基因的进行试验验证。【结果】鉴定出100个具有典型结构域的WRKY,命名为EjWRKY1~EjWRKY100。WRKY长度150~732 aa,分子量介于17189.36~79765.13 Da,等电点(isoelectric point,pI)介于4.92~10.25。亚细胞定位预测结果表明,绝大多数亚细胞定位于细胞核。染色体定位分析显示,其中97个WRKY基因不均匀地分布在17条染色体上。根据WRKY结构域和锌指基序可将枇杷EjWRKY分为3个大类。多数EjWRKY基因在解放钟和山牌3号枇杷果皮中均有表达。EjWRKY基因的qRT-PCR分析结果与转录组数据一致。【结论】鉴定出100个枇杷EjWRKY家族基因,并发现其中部分基因的表达模式与枇杷果皮着色过程显著相关,为进一步研究EjWRKY基因调控枇杷果皮色素合成的分子机制奠定基础。
【Objective】One of the largest transcription factor families in plants that plays an important role in growth,development,and stress responses,WRKYs of Eriobotrya japonica were identified,their expressions examined,and peel pigmentation-related functions determined.【Method】Based on the genome data,EjWRKYs were identified using bioinformatic methods with their characteristics analyzed.Expressions of the genes in the peel pigmentation of Jiefangzhong and Shanpai 3 loquats were determined based on the transcriptome data.qRT-PCR was employed to verify the expressions of some differentially expressed EjWRKYs.【Result】One hundred WRKYs harboring typical domains were identified with the codes of EjWRKY1 to EjWRKY100.The length of EjWRKY ranged 150-732 amino acids with molecular weights ranging 17189.36-79765.13 Da and isoelectric points ranging 4.92-10.25.Most of the identified WRKYs located in the nucleus with 97 of them unevenly distributed in 17 chromosomes.The three domains and zinc-finger motifs-based classes of the proteins were mostly expressed in the peel of Jiefangzhong and Shanpai 3 loquats.The qRT-PCR and RNA-seq analyses showed agreeable results on EjWRKYs.【Conclusion】One hundred EjWRKYs were identified to be associated with the pigmentation of loquat peel.
作者
张雅玲
邓朝军
苏文炳
陈秀萍
蒋际谋
郑少泉
ZHANG Yaling;DENG Chaojun;SU Wenbing;CHEN Xiuping;JIANG Jimou;ZHENG Shaoquan(Fruit Research Institute,Fujian Academy of Agricultural Sciences,Fuzhou,Fujian 350013,China)
出处
《福建农业学报》
北大核心
2025年第5期487-499,共13页
Fujian Journal of Agricultural Sciences
基金
福建省科技计划公益类专项(2022R1028008)
福建省自然科学基金项目(2022J01474)。
关键词
枇杷
WRKY
基因家族
表达分析
果皮着色
Eriobotrya japonica
WRKY
gene family
expression analysis
peel pigmentation
