超高温熔盐泵测试装置是一套用于研究泵、阀、换热器等关键设备在高温熔盐工况下性能的装置。为增强其控制系统的国产化程度及核心控制器的自主可控性,在国产自主指令架构LoongArch上设计研发了基于实验物理与工业控制系统(Experimental...超高温熔盐泵测试装置是一套用于研究泵、阀、换热器等关键设备在高温熔盐工况下性能的装置。为增强其控制系统的国产化程度及核心控制器的自主可控性,在国产自主指令架构LoongArch上设计研发了基于实验物理与工业控制系统(Experimental Physics and Industrial Control System,EPICS)的实时控制器。首先将EPICS、IgH EtherCAT Master等软件移植到基于LoongArch的嵌入式开发板上,解决软件与指令架构不适配的问题,实现控制程序的编写与执行、EtherCAT主从站通讯等功能,并对控制器的最小总线扫描周期进行测试;然后,针对超高温熔盐泵测试装置的控制需求,利用自主研发的EPICS扩展插件在该控制器上实现了PID温度控制、气路流量监测等功能;最后,在实际工况下对控制器的实时性、CPU使用率等指标进行测试分析,评估控制器的性能表现。实验数据表明:该控制器的最小总线扫描周期为50 ms,控制任务执行的延迟时间最大为12.85 ms,CPU性能表现良好,满足该项目的应用需求。该控制器已成功融入超高温熔盐泵测试装置的控制系统,取代了原x86服务器,目前在稳定运行中。展开更多
Cardiac injury initiates repair mechanisms and results in cardiac remodeling and fi-brosis,which appears to be a leading cause of cardiovascular diseases.Cardiac fi-brosis is characterized by the accumulation of extra...Cardiac injury initiates repair mechanisms and results in cardiac remodeling and fi-brosis,which appears to be a leading cause of cardiovascular diseases.Cardiac fi-brosis is characterized by the accumulation of extracellular matrix proteins,mainly collagen in the cardiac interstitium.Many experimental studies have demonstrated that fibrotic injury in the heart is reversible;therefore,it is vital to understand differ-ent molecular mechanisms that are involved in the initiation,progression,and resolu-tion of cardiac fibrosis to enable the development of antifibrotic agents.Of the many experimental models,one of the recent models that has gained renewed interest is isoproterenol(ISP)-induced cardiac fibrosis.ISP is a synthetic catecholamine,sympa-thomimetic,and nonselectiveβ-adrenergic receptor agonist.The overstimulated and sustained activation ofβ-adrenergic receptors has been reported to induce biochemi-cal and physiological alterations and ultimately result in cardiac remodeling.ISP has been used for decades to induce acute myocardial infarction.However,the use of low doses and chronic administration of ISP have been shown to induce cardiac fibrosis;this practice has increased in recent years.Intraperitoneal or subcutaneous ISP has been widely used in preclinical studies to induce cardiac remodeling manifested by fibrosis and hypertrophy.The induced oxidative stress with subsequent perturbations in cellular signaling cascades through triggering the release of free radicals is consid-ered the initiating mechanism of myocardial fibrosis.ISP is consistently used to induce fibrosis in laboratory animals and in cardiomyocytes isolated from animals.In recent years,numerous phytochemicals and synthetic molecules have been evaluated in ISP-induced cardiac fibrosis.The present review exclusively provides a comprehensive summary of the pathological biochemical,histological,and molecular mechanisms of ISP in inducing cardiac fibrosis and hypertrophy.It also summarizes the application of this experimental model in the therapeutic evaluation of natural as well as syn-thetic compounds to demonstrate their potential in mitigating myocardial fibrosis and hypertrophy.展开更多
Neuromyelitis optica spectrum disorders are neuroinflammatory demyelinating disorders that lead to permanent visual loss and motor dysfunction.To date,no effective treatment exists as the exact causative mechanism rem...Neuromyelitis optica spectrum disorders are neuroinflammatory demyelinating disorders that lead to permanent visual loss and motor dysfunction.To date,no effective treatment exists as the exact causative mechanism remains unknown.Therefore,experimental models of neuromyelitis optica spectrum disorders are essential for exploring its pathogenesis and in screening for therapeutic targets.Since most patients with neuromyelitis optica spectrum disorders are seropositive for IgG autoantibodies against aquaporin-4,which is highly expressed on the membrane of astrocyte endfeet,most current experimental models are based on aquaporin-4-IgG that initially targets astrocytes.These experimental models have successfully simulated many pathological features of neuromyelitis optica spectrum disorders,such as aquaporin-4 loss,astrocytopathy,granulocyte and macrophage infiltration,complement activation,demyelination,and neuronal loss;however,they do not fully capture the pathological process of human neuromyelitis optica spectrum disorders.In this review,we summarize the currently known pathogenic mechanisms and the development of associated experimental models in vitro,ex vivo,and in vivo for neuromyelitis optica spectrum disorders,suggest potential pathogenic mechanisms for further investigation,and provide guidance on experimental model choices.In addition,this review summarizes the latest information on pathologies and therapies for neuromyelitis optica spectrum disorders based on experimental models of aquaporin-4-IgG-seropositive neuromyelitis optica spectrum disorders,offering further therapeutic targets and a theoretical basis for clinical trials.展开更多
In the scenario of a steam generator tube rupture accident in a lead-cooled fast reactor,secondary circuit subcooled water under high pressure is injected into an ordinary-pressure primary vessel,where a molten lead-b...In the scenario of a steam generator tube rupture accident in a lead-cooled fast reactor,secondary circuit subcooled water under high pressure is injected into an ordinary-pressure primary vessel,where a molten lead-based alloy(typically pure lead or lead-bismuth eutectic(LBE))is used as the coolant.To clarify the pressure build-up characteristics under water-jet injection,this study conducted several experiments by injecting pressurized water into a molten LBE pool at Sun Yat-sen University.To obtain a further understanding,several new experimental parameters were adopted,including the melt temperature,water subcooling,injection pressure,injection duration,and nozzle diameter.Through detailed analyses,it was found that the pressure and temperature during the water-melt interaction exhibited a consistent variation trend with our previous water-droplet injection mode LBE experiment.Similarly,the existence of a steam explosion was confirmed,which typically results in a much stronger pressure build-up.For the non-explosion cases,increasing the injection pressure,melt-pool temperature,nozzle diameter,and water subcooling promoted pressure build-up in the melt pool.However,a limited enhancement effect was observed when increasing the injection duration,which may be owing to the continually rising pressure in the interaction vessel or the isolation effect of the generated steam cavity.Regardless of whether a steam explosion occurred,the calculated mechanical and kinetic energy conversion efficiencies of the melt were relatively small(not exceeding 4.1%and 0.7%,respectively).Moreover,the range of the conversion efficiency was similar to that of previous water-droplet experiments,although the upper limit of the jet mode was slightly lower.展开更多
【目的】结合超快光学激光器的泵浦-探测实验是硬X射线自由电子激光(Shanghai High Repetition rate XFEL and Extreme light facility,SHINE)的核心实验技术之一,需要精确诊断X射线自由电子激光脉冲和泵浦激光的相对到达时间,并为每发...【目的】结合超快光学激光器的泵浦-探测实验是硬X射线自由电子激光(Shanghai High Repetition rate XFEL and Extreme light facility,SHINE)的核心实验技术之一,需要精确诊断X射线自由电子激光脉冲和泵浦激光的相对到达时间,并为每发X射线自由电子激光脉冲的数据打上BunchID时间戳,从而实现诊断数据与实验数据的联合分析。【方法】本论文的探测器数据采集与控制系统基于实验物理与工业控制系统(Experiment Physics and In-dustrial Control System,EPICS)架构开发,通过基于PyDM(Python Display Manager)的用户界面实现探测器参数配置。该系统分别接收脉冲到达时间诊断设备的探测器图像数据及定时系统的时间标签BunchID,为每帧图像数据打上BunchID时间戳,并将采集数据存储为HDF5格式。【结果】该系统能实时采集探测器数据,准确配置探测器参数,实现探测器图像与BunchID的精确匹配,为SHINE泵浦-探测实验中的到达时间诊断提供了重要的技术支撑。展开更多
In the development framework of engineering colleges,the cultivation of students’practical ability has received unprecedented attention.Based on the actual situation of the experimental teaching of the bridge directi...In the development framework of engineering colleges,the cultivation of students’practical ability has received unprecedented attention.Based on the actual situation of the experimental teaching of the bridge direction of the road and bridge specialty in our school,the targeted teaching experiment reform was carried out,and the comprehensive experiment of the positioning of the crack observation grade steel bar of the reinforced concrete beam was customized,so that the students were fully trained in the application of professional software,experimental hands-on skills,information data analysis and processing,and bridge detection ability.It broadens students’practical ability and professional vision,and lays a good foundation for future work and employment.展开更多
In comparison with conventional experimental teaching methods,the implementation of the Motic digital microscope mutual system in the experimental teaching of medicinal botany has been demonstrated to be a highly effi...In comparison with conventional experimental teaching methods,the implementation of the Motic digital microscope mutual system in the experimental teaching of medicinal botany has been demonstrated to be a highly efficacious approach to enhance the teaching level of experimental courses in medicinal botany.The implementation of a digital microscope mutual system in experimental teaching not only enhances students practical skills in laboratory operations but also increases classroom efficiency.Furthermore,it supports personalized development among students while fostering innovative thinking,independent learning capabilities,and analysis and problem-solving skills.Additionally,this approach contributes to the enhancement of students scientific literacy.展开更多
Airfoil structures play a crucial role across numerous scientific and technological disciplines,with the transition to turbulence and stall onset remaining key challenges in aerodynamic research.While experimental tec...Airfoil structures play a crucial role across numerous scientific and technological disciplines,with the transition to turbulence and stall onset remaining key challenges in aerodynamic research.While experimental techniques often surpass numerical simulations in accuracy,they still present notable limitations.This paper begins by elucidating the fundamental principles of transition,dynamic stall,and airfoil behavior.It then provides a systematic reviewof six major experimentalmethodologies and examines the emerging role of artificial intelligence in this domain.By identifying key challenges and limitations,the study proposes strategic advancements to address these issues,offering a foundational framework to guide future research in airfoil structures and related fields.展开更多
BACKGROUND IgE plays a critical role in allergic inflammation and asthma pathogenesis.This study investigates the involvement of IgE cells in asthma exacerbation and evaluates the effectiveness of targeted interventio...BACKGROUND IgE plays a critical role in allergic inflammation and asthma pathogenesis.This study investigates the involvement of IgE cells in asthma exacerbation and evaluates the effectiveness of targeted interventions.AIM To evaluate the role of IgE in the exacerbation of allergic asthma and to determine the clinical efficacy of anti-IgE therapy in improving disease outcomes.Specifically,the study investigates changes in serum IgE levels,lung function,asthma control scores,and the frequency of acute exacerbations among patients receiving standard therapy with or without anti-IgE intervention.METHODS A total of 200 patients diagnosed with moderate to severe asthma were enrolled in this experimental study conducted from April 2024 to April 2025.Participants were randomized to receive either standard asthma therapy or therapy combined with anti-IgE agents.IgE levels and asthma control parameters were monitored.RESULTS Participants receiving anti-IgE treatment demonstrated a significant reduction in serum IgE levels(P<0.001),improved Forced expiratory volume in one second scores,and fewer exacerbation episodes compared to the control group.CONCLUSION IgE cells significantly contribute to asthma severity,and targeted therapy against IgE can improve disease outcomes.These findings underscore the importance of immunomodulatory strategies in asthma management.展开更多
Background:QiShenYiQi(QSYQ)is commonly accepted to treat ischemic stroke(IS)in clinical settings,yet the underlying mechanism of action of QSYQ is largely unknown.Methods:By combining systems pharmacology with experim...Background:QiShenYiQi(QSYQ)is commonly accepted to treat ischemic stroke(IS)in clinical settings,yet the underlying mechanism of action of QSYQ is largely unknown.Methods:By combining systems pharmacology with experimental assessment,we examined the key targets,bioactive components,and mechanisms of QSYQ against IS.Results:Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform predicted a total number of 254 targets that were potentially related to QSYQ,whereas 699 targets associated with IS were gathered from Therapeutic Target Database,Comparative Toxicogenomics Database,Gene Cards,Online Mendelian Inheritance in Man,and National Center for Biotechnology Information databases,and 83 of these targets overlap with QSYQ-related targets.Importantly,through the analysis of Gene Ontology functional annotation,Kyoto Encyclopedia of Genes and Genomes pathway enrichment,and protein-protein interaction network,we identified 20 related signaling pathways along with 4 hub genes.Subsequently,our molecular docking results revealed that QSYQ might interact with PTGS2,PTGS1,SCN5A,and HSP90AB1.We observed dose-dependent beneficial effects of QSYQ in significantly improving neurological function and alleviating histopathological damage in middle cerebral artery occlusion model,while decreasing infarct volume.Notablely,QSYQ markedly downregulates tumor necrosis factor-α,interleukin-6,and interleukin-1 beta.Overall,this study demonstrates the synergetic effects of QSYQ on regulating multi-targets in IS through inhibiting inflammatory processes and neuronal apoptosis,these findings may expand the understanding of QSYQ and provide guidance for its clinical application in treating IS.Conclusion:Current study reveals the protective roles of QSYQ against IS through modulating PTGS2/PTGS1/SCN5A/HSP90AB1 and TNF signaling pathways.展开更多
A new experimental method is developed to investigate the effect of dissolved substances on the evaporation rate of small water droplets suspended in the atmosphere.The laboratory setup is based on converting a genera...A new experimental method is developed to investigate the effect of dissolved substances on the evaporation rate of small water droplets suspended in the atmosphere.The laboratory setup is based on converting a generated droplet jet of complex structure into a directed flow of evaporating droplets falling in a vertical tube.Images of falling droplets captured by a high-speed camera through a window in the vertical channel wall are used to determine the sizes and velocities of individual droplets.The computational modeling of droplet motion and evaporation proved useful at all stages of the experimental work:from selecting the position of the vertical channel to processing the experimental data.It was found that even a 0.1%mass concentration of the dissolved ionic salt KCl has a considerable effect on decreasing the evaporation rate of the droplets.In contrast,a typical fungicide with a mass concentration of 2.5%has only a slight impact on the evaporation rate.The laboratory results enabled the authors to refine the evaporation model of water droplets to account for the presence of dissolved substances.Modified models of this type are expected to be useful in controling crop spraying and also in other potential applications.展开更多
Cavitation is an unavoidable phenomenon in the operation of centrifugal pumps.Prolonged cavitation can cause significant damage to the components of the flow channel,and in severe cases,it may even interfere with the ...Cavitation is an unavoidable phenomenon in the operation of centrifugal pumps.Prolonged cavitation can cause significant damage to the components of the flow channel,and in severe cases,it may even interfere with the normal energy exchange processes within the pump.Therefore,effective monitoring of cavitation in centrifugal pumps is crucial.This article presents a study that approaches the issue from an acoustic perspective,using experimental methods to gather and analyze acoustic data at the inlet and outlet of centrifugal pumps across various flow rates,with hydrophones as the primary measuring instruments.Results show that flow rate significantly affects noise levels in both non-cavitation and mild cavitation stages,with noise increasing as the flow rate rises.As the cavitation margin(NPSHa)decreases,inlet and outlet noise trends diverge:inlet noise drops sharply,while outlet noise initially increases before sharply decreasing.Both exhibit a distinct zone of abrupt change,where NPSHa values offer earlier cavitation detection than traditional methods.The noise at the pump’s inlet and outlet primarily consists of discrete and broadband noise,with most energy concentrated at discrete frequencies—shaft frequency(24 Hz),blade frequency(144 Hz),and their harmonics.As NPSHa decreases,the inlet’s discrete and broadband noise frequencies decline,while they increase at the outlet.Monitoring changes in these spectrum characteristics provides an additional means of predicting cavitation onset.展开更多
This study investigates the vibration characteristics of bolted-flange-joined conical-cylindrical shells(BFJCCSs)through both theoretical analysis and experimental testing.The proposed model incorporates the pressure ...This study investigates the vibration characteristics of bolted-flange-joined conical-cylindrical shells(BFJCCSs)through both theoretical analysis and experimental testing.The proposed model incorporates the pressure distribution within the bolted joint and accounts for the flange effect.The energy expressions for the conical and cylindrical shells are derived from Donnell's shell theory,while those for the flanges are obtained from the Euler-Bernoulli beam theory.The Lagrange equation is used to derive the dynamic equation,and the experimental studies on the BFJCCS are conducted to validate the accuracy of the model.Subsequently,the comprehensive effects of bolt loosening and bolt number on the frequency parameters are analyzed.Additionally,the effects of the flange dimensions and cone angle on the vibration behavior of the BFJCCS are discussed.In particular,the dynamic differences between the welded conical-cylindrical shell(WCCS)and BFJCCS are investigated.It is found that compared with the WCCS,the fundamental frequency of the BFJCCS is reduced by 7.6%,and the corresponding modal damping ratio is reduced by 21.0%.However,the high-order frequencies of the BFJCCS are higher than those of the WCCS,accompanied by a higher modal damping ratio.Compared with the bolt loosening degree,the bolt number has a more significant effect on frequencies.As the bolt number decreases,the impact of the bolt loosening degree diminishes gradually.展开更多
The insertion and extraction of lithium ions in active materials lead to significant volumetric deformation,resulting in stresses that drive the mechanical degradation of these materials.This accumulation of mechanica...The insertion and extraction of lithium ions in active materials lead to significant volumetric deformation,resulting in stresses that drive the mechanical degradation of these materials.This accumulation of mechanical degradation ultimately leads to mechanical failure in lithium-ion batteries(LIB).This paper summarizes the experimental characterization techniques used to observe the mechanical degradation of lithium battery cells,electrodes,and particles across macro,micro,and nano scales.Additionally,the mechanical failure model for LIB that spans from the microscopic to the macroscopic scale has been outlined.Finally,we analyze the current challenges and opportunities,including the standardization of battery measurements,the quantification of mechanical failures,and the correlation between mechanical failures and electrochemical performance.展开更多
This article aims tomodel and analyze the heat and fluid flow characteristics of a carboxymethyl cellulose(CMC)nanofluid within a convergent-divergent shaped microchannel(Two-dimensional).The base fluid,water+CMC(0.5%...This article aims tomodel and analyze the heat and fluid flow characteristics of a carboxymethyl cellulose(CMC)nanofluid within a convergent-divergent shaped microchannel(Two-dimensional).The base fluid,water+CMC(0.5%),is mixed with CuO and Al2O3 nanoparticles at volume fractions of 0.5%and 1.5%,respectively.The research is conducted through the conjugate usage of experimental and theoretical models to represent more realistic properties of the non-Newtonian nanofluid.Three types of microchannels including straight,divergent,and convergent are considered,all having the same length and identical inlet cross-sectional area.Using ANSYS FLUENT software,Navier-Stokes equations are solved for the laminar flow of the non-Newtonian nanofluid.The study examines the effects of Reynolds number,nanoparticle concentration and type,and microchannel geometry on flow and heat transfer.The results prove that the alumina nanoparticles outperform copper oxide in increasing the Nusselt number at a 0.5% volume fraction,while copper oxide nanoparticles excel at a 1.5%volume fraction.Moreover,in the selected case study,as the Reynolds number increases from 100 to 500,the Nusselt number rises by 56.26% in straight geometry,52.93% in divergent geometry,and 59.10%in convergent geometry.Besides,the Nusselt number enhances by 18.75% when transitioning from straight to convergent geometry at a Reynolds number of 500,and by 19.81%at a Reynolds number of 1000.Finally,the results of the research depict that the use of thermophysical properties derived from the experimental achievements,despite creating complexity in the modeling and the solution method,leads to more accurate and realistic outputs.展开更多
Stripping injection overcomes the limitations of Liouville's theorem and is widely used for beam injection and accumulation in high-intensity synchrotrons.The interaction between the stripping foil and beam is cru...Stripping injection overcomes the limitations of Liouville's theorem and is widely used for beam injection and accumulation in high-intensity synchrotrons.The interaction between the stripping foil and beam is crucial in the study of stripping injection,particularly in low-energy stripping injection synchrotrons,such as the XiPAF synchrotron.The foil thickness is the main parameter that affects the properties of the beam after injection.The thin stripping foil is reinforced with collodion during its installation.However,the collodion on the foil surface makes it difficult to determine its equivalent thickness,because the mechanical measurements are not sufficiently reliable or convenient for continuously determining foil thickness.We propose an online stripping foil thickness measurement method based on the ionization energy loss effect,which is suitable for any foil thickness and does not require additional equipment.Experimental studies were conducted using the XiPAF synchrotron.The limitation of this method was examined,and the results were verified by comparing the experimentally obtained beam current accumulation curves with the simulation results.This confirms the accuracy and reliability of the proposed method for measuring the stripping foil thickness.展开更多
Artificial intelligence(AI)technology is increasingly used in the field of education,but its application in molecular biology experimental teaching still faces challenges.In order to explore the application prospects ...Artificial intelligence(AI)technology is increasingly used in the field of education,but its application in molecular biology experimental teaching still faces challenges.In order to explore the application prospects of AI technology in molecular biology experimental teaching,this paper discusses the application of AI technology in molecular biology experimental teaching,focusing on the construction and application of virtual laboratories.At the same time,the advantages,challenges and future development directions of AI technology application are analyzed.The study found that AI technology has broad application prospects in molecular biology experimental teaching.AI technology can overcome many limitations in traditional experimental teaching,and can also provide personalized learning experience,real-time feedback and evaluation,and simulate complex molecular processes.However,the application of AI technology also faces challenges such as technology cost,teacher training,and curriculum design.In summary,the application of AI technology in molecular biology experimental teaching has significant advantages and can effectively improve teaching quality and learning effects.In the future,we should strengthen the integration of AI technology and traditional teaching methods,develop more AI teaching tools suitable for the characteristics of molecular biology,and focus on cultivating students’practical ability and innovative thinking.This study provides new ideas and directions for promoting the reform and innovation of molecular biology experimental teaching.展开更多
Laminated elastomeric bearings used in seismic isolation rely on the mechanical properties of their constituent elastomers to ensure effective performance.However,despite their resistance to temperature fluctuations a...Laminated elastomeric bearings used in seismic isolation rely on the mechanical properties of their constituent elastomers to ensure effective performance.However,despite their resistance to temperature fluctuations and environmental aggressors,silicone elastomers exhibit relatively low stiffness,limiting their direct applicability in seismic isolation.This study investigates the effect of fumed silica as a reinforcing filler to enhance the mechanical properties of laminated silicone elastomeric bearings.Elastomeric samples were fabricated with varying fumed silica proportions and subjected to Shore A hardness,uniaxial tensile,and lap shear tests to assess the influence of filler content.Additionally,quasi-static tests were conducted on reduced-scale bearing prototypes under combined vertical compression and cyclic horizontal shear to evaluate their seismic isolation performance.The results demonstrate that fumed silica reinforcement significantly increases stiffness,as evidenced by higher Shore A hardness values.However,a trade-off was observed in tensile properties,with reductions in tensile strength and elongation at break.Despite this,the equivalent elastic modulus did not show substantial variation up to large deformations,indicating that stiffness is preserved under most working conditions.Lap shear tests showed that fumed silica improves shear resistance,while quasi-static tests revealed inelastic behavior with small increases in equivalent shear coefficients but no substantial loss in damping ratios.These findings suggest that fumed silica reinforcement enhances silicone elastomers’stiffness and shear resistance while maintaining moderate damping properties,making it a promising approach for improving the mechanical performance of elastomeric bearings in seismic isolation applications.展开更多
文摘超高温熔盐泵测试装置是一套用于研究泵、阀、换热器等关键设备在高温熔盐工况下性能的装置。为增强其控制系统的国产化程度及核心控制器的自主可控性,在国产自主指令架构LoongArch上设计研发了基于实验物理与工业控制系统(Experimental Physics and Industrial Control System,EPICS)的实时控制器。首先将EPICS、IgH EtherCAT Master等软件移植到基于LoongArch的嵌入式开发板上,解决软件与指令架构不适配的问题,实现控制程序的编写与执行、EtherCAT主从站通讯等功能,并对控制器的最小总线扫描周期进行测试;然后,针对超高温熔盐泵测试装置的控制需求,利用自主研发的EPICS扩展插件在该控制器上实现了PID温度控制、气路流量监测等功能;最后,在实际工况下对控制器的实时性、CPU使用率等指标进行测试分析,评估控制器的性能表现。实验数据表明:该控制器的最小总线扫描周期为50 ms,控制任务执行的延迟时间最大为12.85 ms,CPU性能表现良好,满足该项目的应用需求。该控制器已成功融入超高温熔盐泵测试装置的控制系统,取代了原x86服务器,目前在稳定运行中。
基金United Arab Emirates University,Grant/Award Number:12R104 and 12R121。
文摘Cardiac injury initiates repair mechanisms and results in cardiac remodeling and fi-brosis,which appears to be a leading cause of cardiovascular diseases.Cardiac fi-brosis is characterized by the accumulation of extracellular matrix proteins,mainly collagen in the cardiac interstitium.Many experimental studies have demonstrated that fibrotic injury in the heart is reversible;therefore,it is vital to understand differ-ent molecular mechanisms that are involved in the initiation,progression,and resolu-tion of cardiac fibrosis to enable the development of antifibrotic agents.Of the many experimental models,one of the recent models that has gained renewed interest is isoproterenol(ISP)-induced cardiac fibrosis.ISP is a synthetic catecholamine,sympa-thomimetic,and nonselectiveβ-adrenergic receptor agonist.The overstimulated and sustained activation ofβ-adrenergic receptors has been reported to induce biochemi-cal and physiological alterations and ultimately result in cardiac remodeling.ISP has been used for decades to induce acute myocardial infarction.However,the use of low doses and chronic administration of ISP have been shown to induce cardiac fibrosis;this practice has increased in recent years.Intraperitoneal or subcutaneous ISP has been widely used in preclinical studies to induce cardiac remodeling manifested by fibrosis and hypertrophy.The induced oxidative stress with subsequent perturbations in cellular signaling cascades through triggering the release of free radicals is consid-ered the initiating mechanism of myocardial fibrosis.ISP is consistently used to induce fibrosis in laboratory animals and in cardiomyocytes isolated from animals.In recent years,numerous phytochemicals and synthetic molecules have been evaluated in ISP-induced cardiac fibrosis.The present review exclusively provides a comprehensive summary of the pathological biochemical,histological,and molecular mechanisms of ISP in inducing cardiac fibrosis and hypertrophy.It also summarizes the application of this experimental model in the therapeutic evaluation of natural as well as syn-thetic compounds to demonstrate their potential in mitigating myocardial fibrosis and hypertrophy.
文摘Neuromyelitis optica spectrum disorders are neuroinflammatory demyelinating disorders that lead to permanent visual loss and motor dysfunction.To date,no effective treatment exists as the exact causative mechanism remains unknown.Therefore,experimental models of neuromyelitis optica spectrum disorders are essential for exploring its pathogenesis and in screening for therapeutic targets.Since most patients with neuromyelitis optica spectrum disorders are seropositive for IgG autoantibodies against aquaporin-4,which is highly expressed on the membrane of astrocyte endfeet,most current experimental models are based on aquaporin-4-IgG that initially targets astrocytes.These experimental models have successfully simulated many pathological features of neuromyelitis optica spectrum disorders,such as aquaporin-4 loss,astrocytopathy,granulocyte and macrophage infiltration,complement activation,demyelination,and neuronal loss;however,they do not fully capture the pathological process of human neuromyelitis optica spectrum disorders.In this review,we summarize the currently known pathogenic mechanisms and the development of associated experimental models in vitro,ex vivo,and in vivo for neuromyelitis optica spectrum disorders,suggest potential pathogenic mechanisms for further investigation,and provide guidance on experimental model choices.In addition,this review summarizes the latest information on pathologies and therapies for neuromyelitis optica spectrum disorders based on experimental models of aquaporin-4-IgG-seropositive neuromyelitis optica spectrum disorders,offering further therapeutic targets and a theoretical basis for clinical trials.
基金supported by Basic and Applied Basic research foundation of Guangdong province(Nos.2021A1515010343 and 2022A1515011582)the Science and Technology Program of Guangdong Province(Nos.2021A0505030026 and 2022A0505050029).
文摘In the scenario of a steam generator tube rupture accident in a lead-cooled fast reactor,secondary circuit subcooled water under high pressure is injected into an ordinary-pressure primary vessel,where a molten lead-based alloy(typically pure lead or lead-bismuth eutectic(LBE))is used as the coolant.To clarify the pressure build-up characteristics under water-jet injection,this study conducted several experiments by injecting pressurized water into a molten LBE pool at Sun Yat-sen University.To obtain a further understanding,several new experimental parameters were adopted,including the melt temperature,water subcooling,injection pressure,injection duration,and nozzle diameter.Through detailed analyses,it was found that the pressure and temperature during the water-melt interaction exhibited a consistent variation trend with our previous water-droplet injection mode LBE experiment.Similarly,the existence of a steam explosion was confirmed,which typically results in a much stronger pressure build-up.For the non-explosion cases,increasing the injection pressure,melt-pool temperature,nozzle diameter,and water subcooling promoted pressure build-up in the melt pool.However,a limited enhancement effect was observed when increasing the injection duration,which may be owing to the continually rising pressure in the interaction vessel or the isolation effect of the generated steam cavity.Regardless of whether a steam explosion occurred,the calculated mechanical and kinetic energy conversion efficiencies of the melt were relatively small(not exceeding 4.1%and 0.7%,respectively).Moreover,the range of the conversion efficiency was similar to that of previous water-droplet experiments,although the upper limit of the jet mode was slightly lower.
文摘【目的】结合超快光学激光器的泵浦-探测实验是硬X射线自由电子激光(Shanghai High Repetition rate XFEL and Extreme light facility,SHINE)的核心实验技术之一,需要精确诊断X射线自由电子激光脉冲和泵浦激光的相对到达时间,并为每发X射线自由电子激光脉冲的数据打上BunchID时间戳,从而实现诊断数据与实验数据的联合分析。【方法】本论文的探测器数据采集与控制系统基于实验物理与工业控制系统(Experiment Physics and In-dustrial Control System,EPICS)架构开发,通过基于PyDM(Python Display Manager)的用户界面实现探测器参数配置。该系统分别接收脉冲到达时间诊断设备的探测器图像数据及定时系统的时间标签BunchID,为每帧图像数据打上BunchID时间戳,并将采集数据存储为HDF5格式。【结果】该系统能实时采集探测器数据,准确配置探测器参数,实现探测器图像与BunchID的精确匹配,为SHINE泵浦-探测实验中的到达时间诊断提供了重要的技术支撑。
基金Experimental Teaching Reform Project of Liaoning University of Science and Technology,Experimental Teaching Reform of Concrete Member Crack Observation and Reinforcement Location(Project No.:SYJG202419)。
文摘In the development framework of engineering colleges,the cultivation of students’practical ability has received unprecedented attention.Based on the actual situation of the experimental teaching of the bridge direction of the road and bridge specialty in our school,the targeted teaching experiment reform was carried out,and the comprehensive experiment of the positioning of the crack observation grade steel bar of the reinforced concrete beam was customized,so that the students were fully trained in the application of professional software,experimental hands-on skills,information data analysis and processing,and bridge detection ability.It broadens students’practical ability and professional vision,and lays a good foundation for future work and employment.
基金Supported by Major Project of School-level Teaching Reform and Research of Guangxi University of Chinese Medicine(2022A006)。
文摘In comparison with conventional experimental teaching methods,the implementation of the Motic digital microscope mutual system in the experimental teaching of medicinal botany has been demonstrated to be a highly efficacious approach to enhance the teaching level of experimental courses in medicinal botany.The implementation of a digital microscope mutual system in experimental teaching not only enhances students practical skills in laboratory operations but also increases classroom efficiency.Furthermore,it supports personalized development among students while fostering innovative thinking,independent learning capabilities,and analysis and problem-solving skills.Additionally,this approach contributes to the enhancement of students scientific literacy.
基金funded by Programfor Scientific Research Start-up Funds of Guangdong Ocean University,grant number 060302072101Zhanjiang Marine Youth Talent Project Comparative Study and Optimization of Horizontal Lifting of Subsea Pipeline,grant number 2021E5011.
文摘Airfoil structures play a crucial role across numerous scientific and technological disciplines,with the transition to turbulence and stall onset remaining key challenges in aerodynamic research.While experimental techniques often surpass numerical simulations in accuracy,they still present notable limitations.This paper begins by elucidating the fundamental principles of transition,dynamic stall,and airfoil behavior.It then provides a systematic reviewof six major experimentalmethodologies and examines the emerging role of artificial intelligence in this domain.By identifying key challenges and limitations,the study proposes strategic advancements to address these issues,offering a foundational framework to guide future research in airfoil structures and related fields.
文摘BACKGROUND IgE plays a critical role in allergic inflammation and asthma pathogenesis.This study investigates the involvement of IgE cells in asthma exacerbation and evaluates the effectiveness of targeted interventions.AIM To evaluate the role of IgE in the exacerbation of allergic asthma and to determine the clinical efficacy of anti-IgE therapy in improving disease outcomes.Specifically,the study investigates changes in serum IgE levels,lung function,asthma control scores,and the frequency of acute exacerbations among patients receiving standard therapy with or without anti-IgE intervention.METHODS A total of 200 patients diagnosed with moderate to severe asthma were enrolled in this experimental study conducted from April 2024 to April 2025.Participants were randomized to receive either standard asthma therapy or therapy combined with anti-IgE agents.IgE levels and asthma control parameters were monitored.RESULTS Participants receiving anti-IgE treatment demonstrated a significant reduction in serum IgE levels(P<0.001),improved Forced expiratory volume in one second scores,and fewer exacerbation episodes compared to the control group.CONCLUSION IgE cells significantly contribute to asthma severity,and targeted therapy against IgE can improve disease outcomes.These findings underscore the importance of immunomodulatory strategies in asthma management.
基金supported by the National Natural Science Foundation of China(No.82274313)Projects of Shaanxi Administration of Traditional Chinese Medicine(2022-SLRH-YQ-010)Key Laboratory of Traditional Chinese Medicine and Pharmacology.
文摘Background:QiShenYiQi(QSYQ)is commonly accepted to treat ischemic stroke(IS)in clinical settings,yet the underlying mechanism of action of QSYQ is largely unknown.Methods:By combining systems pharmacology with experimental assessment,we examined the key targets,bioactive components,and mechanisms of QSYQ against IS.Results:Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform predicted a total number of 254 targets that were potentially related to QSYQ,whereas 699 targets associated with IS were gathered from Therapeutic Target Database,Comparative Toxicogenomics Database,Gene Cards,Online Mendelian Inheritance in Man,and National Center for Biotechnology Information databases,and 83 of these targets overlap with QSYQ-related targets.Importantly,through the analysis of Gene Ontology functional annotation,Kyoto Encyclopedia of Genes and Genomes pathway enrichment,and protein-protein interaction network,we identified 20 related signaling pathways along with 4 hub genes.Subsequently,our molecular docking results revealed that QSYQ might interact with PTGS2,PTGS1,SCN5A,and HSP90AB1.We observed dose-dependent beneficial effects of QSYQ in significantly improving neurological function and alleviating histopathological damage in middle cerebral artery occlusion model,while decreasing infarct volume.Notablely,QSYQ markedly downregulates tumor necrosis factor-α,interleukin-6,and interleukin-1 beta.Overall,this study demonstrates the synergetic effects of QSYQ on regulating multi-targets in IS through inhibiting inflammatory processes and neuronal apoptosis,these findings may expand the understanding of QSYQ and provide guidance for its clinical application in treating IS.Conclusion:Current study reveals the protective roles of QSYQ against IS through modulating PTGS2/PTGS1/SCN5A/HSP90AB1 and TNF signaling pathways.
基金financially supported by the Russian Science Foundation(project No.24-29-00303:https://rscf.ru/project/24-29-00303/,accessed on 01 July 2025).
文摘A new experimental method is developed to investigate the effect of dissolved substances on the evaporation rate of small water droplets suspended in the atmosphere.The laboratory setup is based on converting a generated droplet jet of complex structure into a directed flow of evaporating droplets falling in a vertical tube.Images of falling droplets captured by a high-speed camera through a window in the vertical channel wall are used to determine the sizes and velocities of individual droplets.The computational modeling of droplet motion and evaporation proved useful at all stages of the experimental work:from selecting the position of the vertical channel to processing the experimental data.It was found that even a 0.1%mass concentration of the dissolved ionic salt KCl has a considerable effect on decreasing the evaporation rate of the droplets.In contrast,a typical fungicide with a mass concentration of 2.5%has only a slight impact on the evaporation rate.The laboratory results enabled the authors to refine the evaporation model of water droplets to account for the presence of dissolved substances.Modified models of this type are expected to be useful in controling crop spraying and also in other potential applications.
基金supported by the National Natural Science Foundation of China(Research Project No.52169018).
文摘Cavitation is an unavoidable phenomenon in the operation of centrifugal pumps.Prolonged cavitation can cause significant damage to the components of the flow channel,and in severe cases,it may even interfere with the normal energy exchange processes within the pump.Therefore,effective monitoring of cavitation in centrifugal pumps is crucial.This article presents a study that approaches the issue from an acoustic perspective,using experimental methods to gather and analyze acoustic data at the inlet and outlet of centrifugal pumps across various flow rates,with hydrophones as the primary measuring instruments.Results show that flow rate significantly affects noise levels in both non-cavitation and mild cavitation stages,with noise increasing as the flow rate rises.As the cavitation margin(NPSHa)decreases,inlet and outlet noise trends diverge:inlet noise drops sharply,while outlet noise initially increases before sharply decreasing.Both exhibit a distinct zone of abrupt change,where NPSHa values offer earlier cavitation detection than traditional methods.The noise at the pump’s inlet and outlet primarily consists of discrete and broadband noise,with most energy concentrated at discrete frequencies—shaft frequency(24 Hz),blade frequency(144 Hz),and their harmonics.As NPSHa decreases,the inlet’s discrete and broadband noise frequencies decline,while they increase at the outlet.Monitoring changes in these spectrum characteristics provides an additional means of predicting cavitation onset.
基金supported by the National Natural Science Foundation of China(No.12272088)the Out-standing Youth Science Foundation of Liaoning Province of China(No.2024JH3/50100013)。
文摘This study investigates the vibration characteristics of bolted-flange-joined conical-cylindrical shells(BFJCCSs)through both theoretical analysis and experimental testing.The proposed model incorporates the pressure distribution within the bolted joint and accounts for the flange effect.The energy expressions for the conical and cylindrical shells are derived from Donnell's shell theory,while those for the flanges are obtained from the Euler-Bernoulli beam theory.The Lagrange equation is used to derive the dynamic equation,and the experimental studies on the BFJCCS are conducted to validate the accuracy of the model.Subsequently,the comprehensive effects of bolt loosening and bolt number on the frequency parameters are analyzed.Additionally,the effects of the flange dimensions and cone angle on the vibration behavior of the BFJCCS are discussed.In particular,the dynamic differences between the welded conical-cylindrical shell(WCCS)and BFJCCS are investigated.It is found that compared with the WCCS,the fundamental frequency of the BFJCCS is reduced by 7.6%,and the corresponding modal damping ratio is reduced by 21.0%.However,the high-order frequencies of the BFJCCS are higher than those of the WCCS,accompanied by a higher modal damping ratio.Compared with the bolt loosening degree,the bolt number has a more significant effect on frequencies.As the bolt number decreases,the impact of the bolt loosening degree diminishes gradually.
基金funded by the Key Research and Development Project of Guangdong Province(No.2023B0909050004)the National Natural Science Foundation of China(No.12402214).
文摘The insertion and extraction of lithium ions in active materials lead to significant volumetric deformation,resulting in stresses that drive the mechanical degradation of these materials.This accumulation of mechanical degradation ultimately leads to mechanical failure in lithium-ion batteries(LIB).This paper summarizes the experimental characterization techniques used to observe the mechanical degradation of lithium battery cells,electrodes,and particles across macro,micro,and nano scales.Additionally,the mechanical failure model for LIB that spans from the microscopic to the macroscopic scale has been outlined.Finally,we analyze the current challenges and opportunities,including the standardization of battery measurements,the quantification of mechanical failures,and the correlation between mechanical failures and electrochemical performance.
文摘This article aims tomodel and analyze the heat and fluid flow characteristics of a carboxymethyl cellulose(CMC)nanofluid within a convergent-divergent shaped microchannel(Two-dimensional).The base fluid,water+CMC(0.5%),is mixed with CuO and Al2O3 nanoparticles at volume fractions of 0.5%and 1.5%,respectively.The research is conducted through the conjugate usage of experimental and theoretical models to represent more realistic properties of the non-Newtonian nanofluid.Three types of microchannels including straight,divergent,and convergent are considered,all having the same length and identical inlet cross-sectional area.Using ANSYS FLUENT software,Navier-Stokes equations are solved for the laminar flow of the non-Newtonian nanofluid.The study examines the effects of Reynolds number,nanoparticle concentration and type,and microchannel geometry on flow and heat transfer.The results prove that the alumina nanoparticles outperform copper oxide in increasing the Nusselt number at a 0.5% volume fraction,while copper oxide nanoparticles excel at a 1.5%volume fraction.Moreover,in the selected case study,as the Reynolds number increases from 100 to 500,the Nusselt number rises by 56.26% in straight geometry,52.93% in divergent geometry,and 59.10%in convergent geometry.Besides,the Nusselt number enhances by 18.75% when transitioning from straight to convergent geometry at a Reynolds number of 500,and by 19.81%at a Reynolds number of 1000.Finally,the results of the research depict that the use of thermophysical properties derived from the experimental achievements,despite creating complexity in the modeling and the solution method,leads to more accurate and realistic outputs.
文摘Stripping injection overcomes the limitations of Liouville's theorem and is widely used for beam injection and accumulation in high-intensity synchrotrons.The interaction between the stripping foil and beam is crucial in the study of stripping injection,particularly in low-energy stripping injection synchrotrons,such as the XiPAF synchrotron.The foil thickness is the main parameter that affects the properties of the beam after injection.The thin stripping foil is reinforced with collodion during its installation.However,the collodion on the foil surface makes it difficult to determine its equivalent thickness,because the mechanical measurements are not sufficiently reliable or convenient for continuously determining foil thickness.We propose an online stripping foil thickness measurement method based on the ionization energy loss effect,which is suitable for any foil thickness and does not require additional equipment.Experimental studies were conducted using the XiPAF synchrotron.The limitation of this method was examined,and the results were verified by comparing the experimentally obtained beam current accumulation curves with the simulation results.This confirms the accuracy and reliability of the proposed method for measuring the stripping foil thickness.
基金Construction Project of Teaching Quality and Teaching Reform Engineering for Undergraduate Universities in Guangdong Province(Project No.:2023-248)First-Class Undergraduate Course in Guangdong Province-Molecular Biology Experiment 2023+1 种基金Curriculum Ideology and Politics Demonstration Project of Lingnan Normal University(Project No.:2022-6&2024-44)Construction Project of Teaching Quality and Teaching Reform Engineering of Lingnan Normal University(Project No.:2024-24)。
文摘Artificial intelligence(AI)technology is increasingly used in the field of education,but its application in molecular biology experimental teaching still faces challenges.In order to explore the application prospects of AI technology in molecular biology experimental teaching,this paper discusses the application of AI technology in molecular biology experimental teaching,focusing on the construction and application of virtual laboratories.At the same time,the advantages,challenges and future development directions of AI technology application are analyzed.The study found that AI technology has broad application prospects in molecular biology experimental teaching.AI technology can overcome many limitations in traditional experimental teaching,and can also provide personalized learning experience,real-time feedback and evaluation,and simulate complex molecular processes.However,the application of AI technology also faces challenges such as technology cost,teacher training,and curriculum design.In summary,the application of AI technology in molecular biology experimental teaching has significant advantages and can effectively improve teaching quality and learning effects.In the future,we should strengthen the integration of AI technology and traditional teaching methods,develop more AI teaching tools suitable for the characteristics of molecular biology,and focus on cultivating students’practical ability and innovative thinking.This study provides new ideas and directions for promoting the reform and innovation of molecular biology experimental teaching.
文摘Laminated elastomeric bearings used in seismic isolation rely on the mechanical properties of their constituent elastomers to ensure effective performance.However,despite their resistance to temperature fluctuations and environmental aggressors,silicone elastomers exhibit relatively low stiffness,limiting their direct applicability in seismic isolation.This study investigates the effect of fumed silica as a reinforcing filler to enhance the mechanical properties of laminated silicone elastomeric bearings.Elastomeric samples were fabricated with varying fumed silica proportions and subjected to Shore A hardness,uniaxial tensile,and lap shear tests to assess the influence of filler content.Additionally,quasi-static tests were conducted on reduced-scale bearing prototypes under combined vertical compression and cyclic horizontal shear to evaluate their seismic isolation performance.The results demonstrate that fumed silica reinforcement significantly increases stiffness,as evidenced by higher Shore A hardness values.However,a trade-off was observed in tensile properties,with reductions in tensile strength and elongation at break.Despite this,the equivalent elastic modulus did not show substantial variation up to large deformations,indicating that stiffness is preserved under most working conditions.Lap shear tests showed that fumed silica improves shear resistance,while quasi-static tests revealed inelastic behavior with small increases in equivalent shear coefficients but no substantial loss in damping ratios.These findings suggest that fumed silica reinforcement enhances silicone elastomers’stiffness and shear resistance while maintaining moderate damping properties,making it a promising approach for improving the mechanical performance of elastomeric bearings in seismic isolation applications.
