This study examines whether ENSTs’teaching goals,teaching contents,teaching methods,and teaching results are effective;whether it is reasonable to appoint ENSTs rather than CNSTs to teach English writing.An ENST’s w...This study examines whether ENSTs’teaching goals,teaching contents,teaching methods,and teaching results are effective;whether it is reasonable to appoint ENSTs rather than CNSTs to teach English writing.An ENST’s writing class from North East Normal University has been selected as the case.Then the effectiveness of the ENSTs’teaching and the students’learning is analyzed.展开更多
Recent findings reveal that long non-coding RNA ENST00000517482(ENST)protects mesenchymal stem cells(MSCs)from mitochondrial apoptosis via the microRNA-539/c-MYC axis,thereby enhancing their paracrine efficacy against...Recent findings reveal that long non-coding RNA ENST00000517482(ENST)protects mesenchymal stem cells(MSCs)from mitochondrial apoptosis via the microRNA-539/c-MYC axis,thereby enhancing their paracrine efficacy against lipopolysaccharide-induced acute lung injury(ALI)in vitro.Furthermore,ENST promotes autophagy through LC3B,autophagy related 7,and autophagy related 5,suggesting a dual role in MSC-mediated lung repair.However,translating these benefits to in vivo applications faces critical challenges.Autophagy,while protective in vitro,may exacerbate epithelial damage during ischemia-reperfusion or hyperoxic ALI if uncontrolled.Additionally,systemic MSC infusion suffers from poor pulmonary engraftment,limiting therapeutic efficiency.To overcome these barriers,future research should prioritize extracellular vehicle-based delivery of ENST-modified MSCs,combined with strategies to fine-tune autophagy via phosphatidylinositol 3-kinase/protein kinase B/mammalian target of the rapamycin or AMP-activated protein kinase/c-Jun-N-terminal kinase modulation.Rigorous validation in endotoxemia and ventilator-induced ALI models,with longitudinal assessment of autophagy and mitochondrial dynamics,will be essential to optimize this approach for clinical translation.展开更多
Objective: Long non-coding RNAs(lnc RNAs) are involved in numerous biological processes in lung cancer cells. In our previous studies, we identified a lnc RNA, ENST00000439577, which is highly expressed in lung carcin...Objective: Long non-coding RNAs(lnc RNAs) are involved in numerous biological processes in lung cancer cells. In our previous studies, we identified a lnc RNA, ENST00000439577, which is highly expressed in lung carcinomas, and termed it lung cancer progression-associated transcript 1(LCPAT1). To characterize the role of LCPAT1 in lung cancer, we conducted the current study.Methods: Expression of LCPAT1 and autophagy-associated markers in tumor tissues and lung cancer cell lines was determined by real-time quantitative polymerase chain reaction(q PCR). Hematoxylin and eosin(HE) staining, q PCR, Western blot, and immunohistochemistry were performed to evaluate xenografted tumor tissues. Autophagy induced by rapamycin was detected by Western blot and immunofluorescence in lung cancer cell lines.Results: Expression of LCPAT1 and microtubule-associated protein 1 light chain 3 beta(LC3B) was positively correlated in lung cancer. Knockdown of LCPAT1 inhibited tumor growth and suppressed cell autophagy in vivo. Moreover, LCPAT1 knockdown in lung cancer cell lines resulted in decreased autophagy-associated gene expression and alleviated the cell autophagy induced by rapamycin.Conclusions: We speculate that LCPAT1 plays a crucial role in regulating autophagy in lung cancer.展开更多
文摘This study examines whether ENSTs’teaching goals,teaching contents,teaching methods,and teaching results are effective;whether it is reasonable to appoint ENSTs rather than CNSTs to teach English writing.An ENST’s writing class from North East Normal University has been selected as the case.Then the effectiveness of the ENSTs’teaching and the students’learning is analyzed.
文摘Recent findings reveal that long non-coding RNA ENST00000517482(ENST)protects mesenchymal stem cells(MSCs)from mitochondrial apoptosis via the microRNA-539/c-MYC axis,thereby enhancing their paracrine efficacy against lipopolysaccharide-induced acute lung injury(ALI)in vitro.Furthermore,ENST promotes autophagy through LC3B,autophagy related 7,and autophagy related 5,suggesting a dual role in MSC-mediated lung repair.However,translating these benefits to in vivo applications faces critical challenges.Autophagy,while protective in vitro,may exacerbate epithelial damage during ischemia-reperfusion or hyperoxic ALI if uncontrolled.Additionally,systemic MSC infusion suffers from poor pulmonary engraftment,limiting therapeutic efficiency.To overcome these barriers,future research should prioritize extracellular vehicle-based delivery of ENST-modified MSCs,combined with strategies to fine-tune autophagy via phosphatidylinositol 3-kinase/protein kinase B/mammalian target of the rapamycin or AMP-activated protein kinase/c-Jun-N-terminal kinase modulation.Rigorous validation in endotoxemia and ventilator-induced ALI models,with longitudinal assessment of autophagy and mitochondrial dynamics,will be essential to optimize this approach for clinical translation.
基金funded by the National Natural Science Foundation of China (Grant No. 81401046 and No.21777099)Shanghai Jiao Tong University Interdisciplinary Research Key Grant (Grant No. YG2015ZD01)Shanghai Jiao Tong University "New Young Teachers Startup Plan"
文摘Objective: Long non-coding RNAs(lnc RNAs) are involved in numerous biological processes in lung cancer cells. In our previous studies, we identified a lnc RNA, ENST00000439577, which is highly expressed in lung carcinomas, and termed it lung cancer progression-associated transcript 1(LCPAT1). To characterize the role of LCPAT1 in lung cancer, we conducted the current study.Methods: Expression of LCPAT1 and autophagy-associated markers in tumor tissues and lung cancer cell lines was determined by real-time quantitative polymerase chain reaction(q PCR). Hematoxylin and eosin(HE) staining, q PCR, Western blot, and immunohistochemistry were performed to evaluate xenografted tumor tissues. Autophagy induced by rapamycin was detected by Western blot and immunofluorescence in lung cancer cell lines.Results: Expression of LCPAT1 and microtubule-associated protein 1 light chain 3 beta(LC3B) was positively correlated in lung cancer. Knockdown of LCPAT1 inhibited tumor growth and suppressed cell autophagy in vivo. Moreover, LCPAT1 knockdown in lung cancer cell lines resulted in decreased autophagy-associated gene expression and alleviated the cell autophagy induced by rapamycin.Conclusions: We speculate that LCPAT1 plays a crucial role in regulating autophagy in lung cancer.
