The infection caused by porcine epidemic diarrhea virus(PEDV)is associated with high mortality in piglets worldwide.Host factors involved in the efficient replication of PEDV,however,remain largely unknown.Our recent ...The infection caused by porcine epidemic diarrhea virus(PEDV)is associated with high mortality in piglets worldwide.Host factors involved in the efficient replication of PEDV,however,remain largely unknown.Our recent proteomic study in the virus-host interaction network revealed a significant increase in the accumulation of CALML5(EF-hand protein calmodulin-like 5)following PEDV infection.A further study unveiled a biphasic increase of CALML5 in 2 and 12 h after viral infection.Similar trends were observed in the intestines of piglets in the early and late stages of the PEDV challenge.Moreover,CALML5 depletion reduced PEDV mRNA and protein levels,leading to a one-order-of-magnitude decrease in virus titer.At the early stage of PEDV infection,CALML5 affected the endosomal trafficking pathway by regulating the expression of endosomal sorting complex related cellular proteins.CALML5 depletion also suppressed IFN-βand IL-6 production in the PEDV-infected cells,thereby indicating its involvement in negatively regulating the innate immune response.Our study reveals the biological function of CALML5 in the virology field and offers new insights into the PEDV-host cell interaction.展开更多
目的:观察亮氨酸拉链EF-hand结构域跨膜蛋白1(leucine zipper/EF-hand-containing transmembrane protein 1,LETM1)在结肠癌中的表达情况,探讨LETM1蛋白是否能够成为判断结肠癌患者预后的新的生物学指标.方法:利用免疫组织化学法检测73...目的:观察亮氨酸拉链EF-hand结构域跨膜蛋白1(leucine zipper/EF-hand-containing transmembrane protein 1,LETM1)在结肠癌中的表达情况,探讨LETM1蛋白是否能够成为判断结肠癌患者预后的新的生物学指标.方法:利用免疫组织化学法检测73例结肠癌患者组织中LETM1蛋白的表达水平和蛋白激酶B(protein kinase B,PKB)/Akt的丝氨酸473(Ser473)、苏氨酸308(Thr308)两个磷酸化位点及葡萄糖合成激酶3β(glycogen synthase k i n a s e 3β,G S K3β)的磷酸化水平,并根据LETM1蛋白表达水平进行高表达和低表达分组;采用Kaplan-Meier法分析各组无疾病生存期;采用χ2检验进行组间单因素分析.结果:在结肠癌患者中LETM1蛋白高表达率为54.8%,LETM1蛋白在结肠癌浸润深度、分化及淋巴结转移中低表达和高表达率分别为27.3%/72.7%、38.3%/61.7%和22.7%/77.3%,其差异均有统计学意义(P<0.05),而与年龄、性别、肿瘤大小未见统计学意义(P>0.05).LETM1蛋白在结肠癌组织中可以激活磷脂酰肌醇3激酶(phosphatidylinositol 3 kinase,PI-3K)/A k t信号传导通路.L ETM1高表达患者无疾病生存期显著低于LETM1低表达患者(P<0.05).结论:LETM1蛋白在结肠癌中高表达,可能参与结肠癌的发生、发展与复发.LETM1蛋白可以作为预测结肠癌患者预后的新的生物学指标.展开更多
基金supported by the National Key R&D Program of China(2023YFD1801100)the National Natural Science Foundation of China(32172821)a CAU-Grant for the Prevention and Control of Immunosuppressive Disease in Animals(CAU-G-PCIDA)of the China Agricultural University.
文摘The infection caused by porcine epidemic diarrhea virus(PEDV)is associated with high mortality in piglets worldwide.Host factors involved in the efficient replication of PEDV,however,remain largely unknown.Our recent proteomic study in the virus-host interaction network revealed a significant increase in the accumulation of CALML5(EF-hand protein calmodulin-like 5)following PEDV infection.A further study unveiled a biphasic increase of CALML5 in 2 and 12 h after viral infection.Similar trends were observed in the intestines of piglets in the early and late stages of the PEDV challenge.Moreover,CALML5 depletion reduced PEDV mRNA and protein levels,leading to a one-order-of-magnitude decrease in virus titer.At the early stage of PEDV infection,CALML5 affected the endosomal trafficking pathway by regulating the expression of endosomal sorting complex related cellular proteins.CALML5 depletion also suppressed IFN-βand IL-6 production in the PEDV-infected cells,thereby indicating its involvement in negatively regulating the innate immune response.Our study reveals the biological function of CALML5 in the virology field and offers new insights into the PEDV-host cell interaction.
文摘目的:观察亮氨酸拉链EF-hand结构域跨膜蛋白1(leucine zipper/EF-hand-containing transmembrane protein 1,LETM1)在结肠癌中的表达情况,探讨LETM1蛋白是否能够成为判断结肠癌患者预后的新的生物学指标.方法:利用免疫组织化学法检测73例结肠癌患者组织中LETM1蛋白的表达水平和蛋白激酶B(protein kinase B,PKB)/Akt的丝氨酸473(Ser473)、苏氨酸308(Thr308)两个磷酸化位点及葡萄糖合成激酶3β(glycogen synthase k i n a s e 3β,G S K3β)的磷酸化水平,并根据LETM1蛋白表达水平进行高表达和低表达分组;采用Kaplan-Meier法分析各组无疾病生存期;采用χ2检验进行组间单因素分析.结果:在结肠癌患者中LETM1蛋白高表达率为54.8%,LETM1蛋白在结肠癌浸润深度、分化及淋巴结转移中低表达和高表达率分别为27.3%/72.7%、38.3%/61.7%和22.7%/77.3%,其差异均有统计学意义(P<0.05),而与年龄、性别、肿瘤大小未见统计学意义(P>0.05).LETM1蛋白在结肠癌组织中可以激活磷脂酰肌醇3激酶(phosphatidylinositol 3 kinase,PI-3K)/A k t信号传导通路.L ETM1高表达患者无疾病生存期显著低于LETM1低表达患者(P<0.05).结论:LETM1蛋白在结肠癌中高表达,可能参与结肠癌的发生、发展与复发.LETM1蛋白可以作为预测结肠癌患者预后的新的生物学指标.
