摘要
目的:观察亮氨酸拉链EF-hand结构域跨膜蛋白1(leucine zipper/EF-hand-containing transmembrane protein 1,LETM1)在结肠癌中的表达情况,探讨LETM1蛋白是否能够成为判断结肠癌患者预后的新的生物学指标.方法:利用免疫组织化学法检测73例结肠癌患者组织中LETM1蛋白的表达水平和蛋白激酶B(protein kinase B,PKB)/Akt的丝氨酸473(Ser473)、苏氨酸308(Thr308)两个磷酸化位点及葡萄糖合成激酶3β(glycogen synthase k i n a s e 3β,G S K3β)的磷酸化水平,并根据LETM1蛋白表达水平进行高表达和低表达分组;采用Kaplan-Meier法分析各组无疾病生存期;采用χ2检验进行组间单因素分析.结果:在结肠癌患者中LETM1蛋白高表达率为54.8%,LETM1蛋白在结肠癌浸润深度、分化及淋巴结转移中低表达和高表达率分别为27.3%/72.7%、38.3%/61.7%和22.7%/77.3%,其差异均有统计学意义(P<0.05),而与年龄、性别、肿瘤大小未见统计学意义(P>0.05).LETM1蛋白在结肠癌组织中可以激活磷脂酰肌醇3激酶(phosphatidylinositol 3 kinase,PI-3K)/A k t信号传导通路.L ETM1高表达患者无疾病生存期显著低于LETM1低表达患者(P<0.05).结论:LETM1蛋白在结肠癌中高表达,可能参与结肠癌的发生、发展与复发.LETM1蛋白可以作为预测结肠癌患者预后的新的生物学指标.
AIM： To observe the expression of leucine zip- per/EF-hand-containing transmembrane pro- tein 1 （LETM1） protein in colonic cancer and to determine whether LETM1 can be used as a marker for this malignancy. METHODS： Immunohistochemical method was used to detect the expression of LETM1 and phosphorylated Akt （protein kinase B, PKB/ Akt） and glycogen synthase kinase 3β （GSK3β）in 73 specimens of colonic carcinoma. Disease- free survival time of colonic cancer patients was analyzed by Kaplan-Meier method. Univariate analysis was performed to determine the correlation between LETM1 pathologic parameters of expression and clinicocolonic cancer. RESULTS： The rate of high LTEM1 protein expression was 54.8% in 73 colonic cancer specimens. LETM1 protein expression was significantly correlated with degree of invasion （27.3% vs 72.7%, P 〈 0.05）, differentiation （38.3% vs 61.7%, P 〈 0.05） and lymph node metastasis （22.7% vs 77.3%, P 〈 0.05）, but not with age, sex or tumor size （P 〉 0.05 for all）. High expression of LETM1 protein could activate the phosphatidylinositol 3 kinase （PI3K）/Akt signal transduction pathway. The disease-flee survival time of patients with high expression of LETM1 was significantly lower than that of patients with low expression of LETM1 （P 〈 0.05）. CONCLUSION： High expression of LETM1 may play a role in the pathogenesis, metastasis and recurrence of colon cancer. LETM1 protein may be used as an indicator to determine the prognosis of colon cancer patients.
出处
《世界华人消化杂志》
CAS
北大核心
2014年第5期718-723,共6页
World Chinese Journal of Digestology
基金
国家自然科学基金资助项目
Nos.81060166
81160270
81160275
高等学校博士学科点专项科研基金资助项目
No.20102201120003~~
