Intrathecal administration of human umbilical cord mesenchymal stem cells may be a promising approach for the treatment of stroke,but its safety,effectiveness,and mechanism remain to be elucidated.In this study,good m...Intrathecal administration of human umbilical cord mesenchymal stem cells may be a promising approach for the treatment of stroke,but its safety,effectiveness,and mechanism remain to be elucidated.In this study,good manufacturing practice-grade human umbilical cord mesenchymal stem cells(5×105 and 1×106 cells)and saline were administered by cerebellomedullary cistern injection 72 hours after stroke induced by middle cerebral artery occlusion in rats.The results showed(1)no significant difference in mortality or general conditions among the three groups.There was no abnormal differentiation or tumor formation in various organs of rats in any group.(2)Compared with saline-treated animals,those treated with human umbilical cord mesenchymal stem cells showed significant functional recovery and reduced infarct volume,with no significant differences between different human umbilical cord mesenchymal stem cell doses.(3)Human umbilical cord mesenchymal stem cells were found in the ischemic brain after 14 and 28 days of follow-up,and the number of positive cells significantly decreased over time.(4)Neuronal nuclei expression in the human umbilical cord mesenchymal stem cell group was greater than that in the saline group,while glial fibrillary acidic protein and ionized calcium binding adaptor molecule 1 expression levels decreased.(5)Human umbilical cord mesenchymal stem cell treatment increased the number of CD31+microvessels and doublecortin-positive cells after ischemic stroke.Human umbilical cord mesenchymal stem cells also upregulated the expression of CD31+/Ki67+.(6)At 14 days after intrathecal administration,brain-derived neurotrophic factor expression in the peri-infarct area and the concentrations of brain-derived neurotrophic factor in the cerebrospinal fluid in both human umbilical cord mesenchymal stem cell groups were significantly greater than those in the saline group and persisted until the 28th day.Taken together,these results indicate that the intrathecal administration of human umbilical cord mesenchymal stem cells via cerebellomedullary cistern injection is safe and effective for the treatment of ischemic stroke in rats.The mechanisms may include alleviating the local inflammatory response in the peri-infarct region,promoting neurogenesis and angiogenesis,and enhancing the production of neurotrophic factors.展开更多
The utilization of nuclear power will persist as a prominent energy source in the foreseeable future.However,it presents substantial challenges concerning waste disposal and the potential emission of untreated radioac...The utilization of nuclear power will persist as a prominent energy source in the foreseeable future.However,it presents substantial challenges concerning waste disposal and the potential emission of untreated radioactive substances,such as radioactive 129I and 131I.The transportation of radioactive iodine poses a significant threat to both the environment and human health.Nevertheless,effectively,rapidly removing iodine ion from water using porous adsorbents remains a crucial challenge.In this work,three kinds of multiple sites porous organic polymers(POPs,POP-1,POP-2,and POP-3)have been developed using a monomer pre-modification strategy for highly efficient and fast I_(3) absorption from water.It is found that the POPs exhibited exceptional performance in terms of I3 adsorption,achieving a top-performing adsorption capacity of 5.25 g g^(-1) and the fastest average adsorption rate(K_(80%)=4.25 g g^(-1) h^(-1))with POP-1.Moreover,POP-1 exhibited exceptional capacity for the removal of I3 fromflowing aqueous solutions,with 95%removal efficiency observed even at 0.0005 mol L^(-1).Such results indicate that this material has the potential to be utilized for the emergency preparation of potable water in areas contaminated with radioactive iodine.The adsorption process can be effectively characterized by the Freundlich model and the pseudo-second-order model.The exceptional I_(3) absorption capacity is primarily attributed to the incorporation of a substantial number of active adsorption sites,including bromine,carbonyl,and amide groups.展开更多
Using ground-based telescopes,the multi-color photometric observations of the contact binary EF Boo were obtained in 2020,2023,and 2024.Combining these with 7-sectors of light curves from TESS data,the variations of t...Using ground-based telescopes,the multi-color photometric observations of the contact binary EF Boo were obtained in 2020,2023,and 2024.Combining these with 7-sectors of light curves from TESS data,the variations of the O'Connell effect in continuous time and shapes of light curves over several years were identified.Three sets of typical light curves were analyzed to determine the photometric solutions via the Wilson-Devinney program.Considering the spectroscopic mass ratio of q=0.53,these photometric solutions suggest that EF Boo is a W-type W UMa contact binary with the averaged filling factor of f=22.26%,a small temperature difference,and a cool spot on the primary component.If the variations of the O'Connell effect are due to the magnetic activity of this cool spot,the longitudinal location varied from 50.4 to 302.7 over the time interval of 1434 days.Based on all CCD minimum times from ground-based telescope and TESS data,the O-C curve was also analyzed.A cyclic oscillation(A3=0.00575 days,T3=27.8 yr)superimposed on a secular increase(dP/dt=6.74×10^(-8)day yr^(-1))was discovered for the first time.The successive increase is possibly a result of mass transfer from the less massive star to the more massive one.The cyclic oscillations were possibly explained by the light-travel time effect via a third body or the magnetic activities.From the short cadence observations from TESS,we also calculated the value of the O'Connell effect and O-C value for each cycle and found no correlation between the O'Connell effect and O-C over nearly 30 days across different sectors.展开更多
基金supported by the Medicine-Engineering Interdisciplinary Project of Sun Yat-sen Memorial Hospital,China,No.YXYGRH202203(to YW)Key-Area Research and Development Program of Guangdong Province,China,No.2023B1111050003(to HC)Guangzhou Science and Technology Talent Project of China,No.201909020006(to HC).
文摘Intrathecal administration of human umbilical cord mesenchymal stem cells may be a promising approach for the treatment of stroke,but its safety,effectiveness,and mechanism remain to be elucidated.In this study,good manufacturing practice-grade human umbilical cord mesenchymal stem cells(5×105 and 1×106 cells)and saline were administered by cerebellomedullary cistern injection 72 hours after stroke induced by middle cerebral artery occlusion in rats.The results showed(1)no significant difference in mortality or general conditions among the three groups.There was no abnormal differentiation or tumor formation in various organs of rats in any group.(2)Compared with saline-treated animals,those treated with human umbilical cord mesenchymal stem cells showed significant functional recovery and reduced infarct volume,with no significant differences between different human umbilical cord mesenchymal stem cell doses.(3)Human umbilical cord mesenchymal stem cells were found in the ischemic brain after 14 and 28 days of follow-up,and the number of positive cells significantly decreased over time.(4)Neuronal nuclei expression in the human umbilical cord mesenchymal stem cell group was greater than that in the saline group,while glial fibrillary acidic protein and ionized calcium binding adaptor molecule 1 expression levels decreased.(5)Human umbilical cord mesenchymal stem cell treatment increased the number of CD31+microvessels and doublecortin-positive cells after ischemic stroke.Human umbilical cord mesenchymal stem cells also upregulated the expression of CD31+/Ki67+.(6)At 14 days after intrathecal administration,brain-derived neurotrophic factor expression in the peri-infarct area and the concentrations of brain-derived neurotrophic factor in the cerebrospinal fluid in both human umbilical cord mesenchymal stem cell groups were significantly greater than those in the saline group and persisted until the 28th day.Taken together,these results indicate that the intrathecal administration of human umbilical cord mesenchymal stem cells via cerebellomedullary cistern injection is safe and effective for the treatment of ischemic stroke in rats.The mechanisms may include alleviating the local inflammatory response in the peri-infarct region,promoting neurogenesis and angiogenesis,and enhancing the production of neurotrophic factors.
基金support from the National Natural Science Foundation of China(No.22273016,22273017,22233006)Plan for Henan Province University Science and Technology Innovation Team(No.25IRTSTHN002)+1 种基金Young Backbone Teacher Training Program of Henan Province(2023GGJS036)the 111 project(No.D17007).
文摘The utilization of nuclear power will persist as a prominent energy source in the foreseeable future.However,it presents substantial challenges concerning waste disposal and the potential emission of untreated radioactive substances,such as radioactive 129I and 131I.The transportation of radioactive iodine poses a significant threat to both the environment and human health.Nevertheless,effectively,rapidly removing iodine ion from water using porous adsorbents remains a crucial challenge.In this work,three kinds of multiple sites porous organic polymers(POPs,POP-1,POP-2,and POP-3)have been developed using a monomer pre-modification strategy for highly efficient and fast I_(3) absorption from water.It is found that the POPs exhibited exceptional performance in terms of I3 adsorption,achieving a top-performing adsorption capacity of 5.25 g g^(-1) and the fastest average adsorption rate(K_(80%)=4.25 g g^(-1) h^(-1))with POP-1.Moreover,POP-1 exhibited exceptional capacity for the removal of I3 fromflowing aqueous solutions,with 95%removal efficiency observed even at 0.0005 mol L^(-1).Such results indicate that this material has the potential to be utilized for the emergency preparation of potable water in areas contaminated with radioactive iodine.The adsorption process can be effectively characterized by the Freundlich model and the pseudo-second-order model.The exceptional I_(3) absorption capacity is primarily attributed to the incorporation of a substantial number of active adsorption sites,including bromine,carbonyl,and amide groups.
基金support of the staff of the 85 cm,60 cm telescopes at the Xinglong observational station of the National Astronomical Observatories,Chinese Academy of Sciences,and TESS team works funding by the NASA Science Mission directorate.This work is sponsored by the Natural Science Foundation of Xinjiang Uygur Autonomous Region(No.2022D01A164)the National Natural Science Foundation of China(Nos.U1831109 and 12103030)。
文摘Using ground-based telescopes,the multi-color photometric observations of the contact binary EF Boo were obtained in 2020,2023,and 2024.Combining these with 7-sectors of light curves from TESS data,the variations of the O'Connell effect in continuous time and shapes of light curves over several years were identified.Three sets of typical light curves were analyzed to determine the photometric solutions via the Wilson-Devinney program.Considering the spectroscopic mass ratio of q=0.53,these photometric solutions suggest that EF Boo is a W-type W UMa contact binary with the averaged filling factor of f=22.26%,a small temperature difference,and a cool spot on the primary component.If the variations of the O'Connell effect are due to the magnetic activity of this cool spot,the longitudinal location varied from 50.4 to 302.7 over the time interval of 1434 days.Based on all CCD minimum times from ground-based telescope and TESS data,the O-C curve was also analyzed.A cyclic oscillation(A3=0.00575 days,T3=27.8 yr)superimposed on a secular increase(dP/dt=6.74×10^(-8)day yr^(-1))was discovered for the first time.The successive increase is possibly a result of mass transfer from the less massive star to the more massive one.The cyclic oscillations were possibly explained by the light-travel time effect via a third body or the magnetic activities.From the short cadence observations from TESS,we also calculated the value of the O'Connell effect and O-C value for each cycle and found no correlation between the O'Connell effect and O-C over nearly 30 days across different sectors.
