[Objective] This study aimed to understand the genetic diversity and breed conservation effect, and to provide reference for future germplasm resources protection and breeding researches of Guangxi Donglan black-bone ...[Objective] This study aimed to understand the genetic diversity and breed conservation effect, and to provide reference for future germplasm resources protection and breeding researches of Guangxi Donglan black-bone chicken. [Method] The wing venous blood of Donglan Black-bone chicken（silky feather and contour feather） was collected, and the total DNA was extracted. The 18 microsatellite loci were amplified by PCR technology, and the obtained gene fragments were scanned. The number of alleles（Na）, number of effective alleles（Ne）, heterozygosity（H） and polymorphism information content（PIC） were determined using Pop Gen 32 biological software to do cluster analysis of Guangxi indigenous chicken breeds. [Result] The18 microsatellite loci of Donglan black-bone chicken were found to be highly polymorphic. A total of 98 alleles were detected out from 80 Donglan black-bone chicken samples. The averages of Na, Ne, PIC and H per locus were 5.44, 2.78, 0.5382 and 0.596 3, respectively. A total of 93 and 98 alleles were detected out from silky- and contour-feather Donglan black-bone chickens, respectively. The averages of Ne per locus were 2.62 and 2.76, of PIC per locus were 0.508 8 and 0.539 8,of H per locus were 0.624 7 and 0.599 1, respectively. The results of cluster analysis showed that there was greater genetic distance between Donglan black-bone chicken and any of other Guangxi indigenous chicken breeds, including three yellow chicken, Xiayan chicken and Nandan Yao chicken, which was consistent with the breed evolution, geographical distribution and breeding history of Donglan black-bone chicken. [Conclusion] There are greater genetic variation and abundant genetic diversity in the conserved population of Donglan black-bone chicken, suggesting abundant genetic diversity and great breeding potential of Donglan black-bone chicken.展开更多
In this study, PCR-RFLP technique was employed to detect the genetic polymorphism of NPY gene and analyze the effects of various genotypes on the total number of eggs at 300 days of age in 135 Donglan black-bone chick...In this study, PCR-RFLP technique was employed to detect the genetic polymorphism of NPY gene and analyze the effects of various genotypes on the total number of eggs at 300 days of age in 135 Donglan black-bone chicken. According to the results, there were three genotypes （AA, AB and BB） of NPYgene in Donglan black-bone chicken group. Different genotypes exhibited significant effects （P 〈 0. 05 ） on the total number of eggs at 300 days of age. The total number of eggs at 300 days of age of AA genotype was significantly higher than that of BB genotype （P 〈 0. 05）. Therefore, the polymorphic site of NPY gene could be used as a candidate molecular marker that affects egg laying in Donglan black-bone chicken.展开更多
[Objectives]The study was conducted to evaluate the effects of diet with"Lianhuang"additive of Chinese herbal medicine(LACHM)on growth performance and metabolic rates of dietary nutrients in Donglan black ch...[Objectives]The study was conducted to evaluate the effects of diet with"Lianhuang"additive of Chinese herbal medicine(LACHM)on growth performance and metabolic rates of dietary nutrients in Donglan black chickens(DBCs).[Methods]80 DBCs aged 1 d were blocked into 4 groups randomly and assigned to 6 replicates in each group with 20 chicks in each replicate.Group A as control group fed basal diet.Groups B,C and D as treatment groups consumed the basal diet added with 0.25%,0.50%and 1.00%of LACHM,respectively during the test period of 70 d.In the end of feeding test,24 DBCs with similar body weight from each group were chosen out and used for a metabolism test to determine the metabolic rates of dietary nutrients from each treatment.The metabolism test was conducted as the same design as the feeding test and the DBCs from each group were fed the same diet as previous test.[Results]The ADG of chickens in the test increased by 5.00%-9.15%(P<0.05)except the stage of 50-70 d,and the individual average body weight(IABW)at 70 d in group B also increased by 4.86%(P<0.05).Compared with group A,the ADG of group C in the stages of 36-49,1-49 and 1-70 d and the IABW in the end of 70 d increased by 5.90%,2.66%,2.68%and 2.57%(P>0.05),respectively.The ADFI in the stage of 36-49 d from group A was 6.04%more than that from group B(P<0.01),and 5.12%and 4.13%more than those from groups C and D(P<0.05),respectively,and the ADFI of group A in the stage of 1-49 d was still 2.86%more than that from group C(P<0.05).However,no differences in ADFI were found in other stages in the test among the 4 groups(P>0.05).The F/G in the stages of 22-35 and 1-70 d from group B was 6.32%and 3.41%lower than those from group A(P<0.05),respectively,and even 11.43%and 7.31%lower in the stages of 36-49 and 1-49 d from group B than those from group A(P<0.01),respectively.The F/G of group C in the stage of 36-49 d was also 10.15%lower than that of group A(P<0.05),and still 5.29%lower than that from group A in 1-49 d(P<0.01),but no differences were found in other stages between the two groups(P>0.05).The metabolic rates of MD,CP,CF,NFE,CA,TE,Ca and P in the 3 treatment diets for group B,C and D all increased to some extent.Among them,the increasing extents of diets with 0.25%and 0.50%of LACHM for groups B and C were much greater.Therefore,the diet with 0.25%of LACHM could improve the growth performance and metabolic rates of dietary nutrients in DBCs significantly.[Conclusions]This study provides a theoretical reference for the effects of traditional Chinese medicine additives on the growth and metabolic rates of DBCs.展开更多
With the rapid development of agricultural industry, people have higher and higher requirements for the safety and quality of agricultural products, and rice, as one of the traditional food crops in China, has also re...With the rapid development of agricultural industry, people have higher and higher requirements for the safety and quality of agricultural products, and rice, as one of the traditional food crops in China, has also received extensive attention. Rice is the main food crop in China and plays an important role in Chinas agricultural production. Whether rice is planted green or not will have a significant impact on peoples health, and green rice planting will gradually become the future development trend of rice production. At present, the green ecological technology is developing rapidly in China, and the rice planting technology in China is constantly improving and perfecting, which can not only realize the greening and scale of rice production, but also improve the quality of rice planting and realize the sustainable development and progress of Chinas agriculture. In this paper, taking Donglan County as the main research background, the cultivation points and popularization measures of rice green high-yield technology are analyzed, which will provide theoretical basis for further popularization of rice green high-yield technology.展开更多
为探讨发酵桂闽引象草替代部分日粮对鸡肠道菌群的影响,试验选定30日龄的东兰乌鸡4羽,随机分成4组,每组1羽。使用发酵桂闽引象草分别替代5%(1组)、10%(2组)、15%(3组)及0%(K组)的日粮;以1、2、3组为试验组,K组为对照组,进行饲养试验。...为探讨发酵桂闽引象草替代部分日粮对鸡肠道菌群的影响,试验选定30日龄的东兰乌鸡4羽,随机分成4组,每组1羽。使用发酵桂闽引象草分别替代5%(1组)、10%(2组)、15%(3组)及0%(K组)的日粮;以1、2、3组为试验组,K组为对照组,进行饲养试验。采集鸡大肠内容物,对其细菌16S r DNA扩增子V3~V4区进行二代高通量测序,分析菌群的物种组成及相对丰度差异。结果表明:Chao1指数,2组(813.500)>3组(782.892)>1组(765.444)>K组(751.243);Shannon指数,K组(7.386)>2组(7.327)>3组(7.098)>1组(6.968)。从chao1指数来看,物种丰富度最高的是2组,最低的是K组;三个试验组的物种丰富度均高于对照组。从Shannon指数来看,对照组的菌群多样性高于试验组;三个试验组的菌群多样性以2组最高,以1组最低。拟杆菌门和厚壁菌门在1、2、3、K组的相对丰度分别为43.59%、55.33%、56.84%、56.01%和49.89%、40.32%、38.79%、39.95%,在各组中占比分别为93.48%、95.65%、95.63%、95.96%,是四组中的优势门,试验组和对照组中的相对丰度差异不明显。变形菌门在1、2、3组中的相对丰度(4.08%、1.41%、2.05%)高于对照组(0.97%),分别为对照组的4.2、1.45、2.11倍。互养菌门在1、2、3组中的相对丰度(0.72%、0.9%、0.49%)高于对照组(0.32%),分别为对照组的2.25、2.81、1.53倍。相对丰度前10位的属隶属于两个门(拟杆菌门和厚壁菌门)。多形杆状菌属在各组中的相对丰度分别为28.4%、29.4%、31.84%、28.23%,是四组中的优势属;其相对丰度在试验组和对照组中差异不明显。瘤胃球菌属和巨单胞菌属在1、2、3组中的相对丰度(8.03%、5.21%、3.97%和5.44%、2.05%、2.06%)高于对照组(2.44%和0.15%),分别是对照组的3.29、2.19、2.20倍和36.26、13.66、13.73倍。用发酵桂闽引象草替代部分日粮,鸡肠道菌群丰富度增高,多样性降低,对鸡肠道优势菌影响不明显,最主要的影响是变形菌门、互养菌门的数量增多及厚壁菌门中的瘤胃球菌属、巨单胞菌属的数量增多。展开更多
[Objectives]To study the effects of Chinese herbal medicine feed additives on the growth performance and dietary nutrient metabolic rate of black-one chickens.[Methods]480 pieces of 1-day-old Donglan Black-bone Chicke...[Objectives]To study the effects of Chinese herbal medicine feed additives on the growth performance and dietary nutrient metabolic rate of black-one chickens.[Methods]480 pieces of 1-day-old Donglan Black-bone Chickens were selected and divided into 4 groups,with 6 replicates in each group,with 20 pieces in each replicate.Among them,group A was the control group and was fed the basal diet;the groups B,C,and D were the experimental groups,which were separately supplemented with 0.25%,0.50%and 1.00%of Chinese herbal medicine additives in the basal diet,and the experimental period was 70 d.At the end of the feeding experiment,24 experimental chickens with similar body weight were selected from each group,and the same design of the feeding experiment was used to continue to feed the original diet for the metabolic experiment to determine the nutrient metabolic rate.[Results]Except the 50-70 d stage,the average daily gain(ADG)of group B in other stages was increased by 5.00%-9.15%(P<0.05),and the average body weight of group B at 70 d was also increased by 4.86%(P<0.05);the ADG of group C at 36-49,1-49 and 1-70 d and the average individual weight at 70 d were also higher than that of group A by 5.90%,2.66%,2.68%and 2.57%,respectively(P>0.05);at 36-49 d,the average daily feed intake(ADFI)of group A was higher than that of group B by 6.04%(P<0.01),and was also higher than that of groups C and D by 5.12%and 4.13%(P<0.05),respectively;at 1-49 d,the ADFI of group A was higher than that of group C by 2.86%(P<0.05),and there was no significant difference between the four groups of experimental chickens at each stage(P>0.05);at 22-35 and 1-70 d,the feed to gain ratio(F/G)of group B was 6.32%and 3.41%lower than that of group A(P<0.05),and at 36-49 and 1-49 d,the F/G of group B was even lower;at 36-49 d,the F/G of group C was 10.15%lower than that of group A(P<0.05),and at 1-49 d,it was also lower than that of group A by 5.29%(P<0.01);there was no significant difference between the two groups at other stages.In terms of nutrient metabolism:the three different amounts of this additive have increased the metabolic rate of DM,CP,CF,NFE,CA,Ca,P,TE and other nutrients in the diet to varying degrees,especially the addition of 0.25%and 0.50%had the significant increase.[Conclusions]The addition of 0.25%Chinese herbal medicine additives in the diet can significantly improve the growth performance and main nutrient metabolic rate of Donglan Black-bone Chickens.展开更多
基金Supported by Guangxi Natural Science Foundation(2013GXNSFDA019013)Fundamental Research Fund of Guangxi Institute of Animal Science(GMYK2014-05)~~
文摘[Objective] This study aimed to understand the genetic diversity and breed conservation effect, and to provide reference for future germplasm resources protection and breeding researches of Guangxi Donglan black-bone chicken. [Method] The wing venous blood of Donglan Black-bone chicken（silky feather and contour feather） was collected, and the total DNA was extracted. The 18 microsatellite loci were amplified by PCR technology, and the obtained gene fragments were scanned. The number of alleles（Na）, number of effective alleles（Ne）, heterozygosity（H） and polymorphism information content（PIC） were determined using Pop Gen 32 biological software to do cluster analysis of Guangxi indigenous chicken breeds. [Result] The18 microsatellite loci of Donglan black-bone chicken were found to be highly polymorphic. A total of 98 alleles were detected out from 80 Donglan black-bone chicken samples. The averages of Na, Ne, PIC and H per locus were 5.44, 2.78, 0.5382 and 0.596 3, respectively. A total of 93 and 98 alleles were detected out from silky- and contour-feather Donglan black-bone chickens, respectively. The averages of Ne per locus were 2.62 and 2.76, of PIC per locus were 0.508 8 and 0.539 8,of H per locus were 0.624 7 and 0.599 1, respectively. The results of cluster analysis showed that there was greater genetic distance between Donglan black-bone chicken and any of other Guangxi indigenous chicken breeds, including three yellow chicken, Xiayan chicken and Nandan Yao chicken, which was consistent with the breed evolution, geographical distribution and breeding history of Donglan black-bone chicken. [Conclusion] There are greater genetic variation and abundant genetic diversity in the conserved population of Donglan black-bone chicken, suggesting abundant genetic diversity and great breeding potential of Donglan black-bone chicken.
基金Supported by Natural Science Foundation of Guangxi Zhuang Autonomous Region(2013jj DA30049)
文摘In this study, PCR-RFLP technique was employed to detect the genetic polymorphism of NPY gene and analyze the effects of various genotypes on the total number of eggs at 300 days of age in 135 Donglan black-bone chicken. According to the results, there were three genotypes （AA, AB and BB） of NPYgene in Donglan black-bone chicken group. Different genotypes exhibited significant effects （P 〈 0. 05 ） on the total number of eggs at 300 days of age. The total number of eggs at 300 days of age of AA genotype was significantly higher than that of BB genotype （P 〈 0. 05）. Therefore, the polymorphic site of NPY gene could be used as a candidate molecular marker that affects egg laying in Donglan black-bone chicken.
基金Supported by Guangxi Innovation-driven Development Major Project(GK AA17204024)Guangxi Key Research and Development Program(GK AB18294038)Guangxi Broiler Innovation Team Construction Project of National Modern Agricultural Industry Technology System(nycytxgxcxtd-19-01)。
文摘[Objectives]The study was conducted to evaluate the effects of diet with"Lianhuang"additive of Chinese herbal medicine(LACHM)on growth performance and metabolic rates of dietary nutrients in Donglan black chickens(DBCs).[Methods]80 DBCs aged 1 d were blocked into 4 groups randomly and assigned to 6 replicates in each group with 20 chicks in each replicate.Group A as control group fed basal diet.Groups B,C and D as treatment groups consumed the basal diet added with 0.25%,0.50%and 1.00%of LACHM,respectively during the test period of 70 d.In the end of feeding test,24 DBCs with similar body weight from each group were chosen out and used for a metabolism test to determine the metabolic rates of dietary nutrients from each treatment.The metabolism test was conducted as the same design as the feeding test and the DBCs from each group were fed the same diet as previous test.[Results]The ADG of chickens in the test increased by 5.00%-9.15%(P<0.05)except the stage of 50-70 d,and the individual average body weight(IABW)at 70 d in group B also increased by 4.86%(P<0.05).Compared with group A,the ADG of group C in the stages of 36-49,1-49 and 1-70 d and the IABW in the end of 70 d increased by 5.90%,2.66%,2.68%and 2.57%(P>0.05),respectively.The ADFI in the stage of 36-49 d from group A was 6.04%more than that from group B(P<0.01),and 5.12%and 4.13%more than those from groups C and D(P<0.05),respectively,and the ADFI of group A in the stage of 1-49 d was still 2.86%more than that from group C(P<0.05).However,no differences in ADFI were found in other stages in the test among the 4 groups(P>0.05).The F/G in the stages of 22-35 and 1-70 d from group B was 6.32%and 3.41%lower than those from group A(P<0.05),respectively,and even 11.43%and 7.31%lower in the stages of 36-49 and 1-49 d from group B than those from group A(P<0.01),respectively.The F/G of group C in the stage of 36-49 d was also 10.15%lower than that of group A(P<0.05),and still 5.29%lower than that from group A in 1-49 d(P<0.01),but no differences were found in other stages between the two groups(P>0.05).The metabolic rates of MD,CP,CF,NFE,CA,TE,Ca and P in the 3 treatment diets for group B,C and D all increased to some extent.Among them,the increasing extents of diets with 0.25%and 0.50%of LACHM for groups B and C were much greater.Therefore,the diet with 0.25%of LACHM could improve the growth performance and metabolic rates of dietary nutrients in DBCs significantly.[Conclusions]This study provides a theoretical reference for the effects of traditional Chinese medicine additives on the growth and metabolic rates of DBCs.
文摘With the rapid development of agricultural industry, people have higher and higher requirements for the safety and quality of agricultural products, and rice, as one of the traditional food crops in China, has also received extensive attention. Rice is the main food crop in China and plays an important role in Chinas agricultural production. Whether rice is planted green or not will have a significant impact on peoples health, and green rice planting will gradually become the future development trend of rice production. At present, the green ecological technology is developing rapidly in China, and the rice planting technology in China is constantly improving and perfecting, which can not only realize the greening and scale of rice production, but also improve the quality of rice planting and realize the sustainable development and progress of Chinas agriculture. In this paper, taking Donglan County as the main research background, the cultivation points and popularization measures of rice green high-yield technology are analyzed, which will provide theoretical basis for further popularization of rice green high-yield technology.
文摘为探讨发酵桂闽引象草替代部分日粮对鸡肠道菌群的影响,试验选定30日龄的东兰乌鸡4羽,随机分成4组,每组1羽。使用发酵桂闽引象草分别替代5%(1组)、10%(2组)、15%(3组)及0%(K组)的日粮;以1、2、3组为试验组,K组为对照组,进行饲养试验。采集鸡大肠内容物,对其细菌16S r DNA扩增子V3~V4区进行二代高通量测序,分析菌群的物种组成及相对丰度差异。结果表明:Chao1指数,2组(813.500)>3组(782.892)>1组(765.444)>K组(751.243);Shannon指数,K组(7.386)>2组(7.327)>3组(7.098)>1组(6.968)。从chao1指数来看,物种丰富度最高的是2组,最低的是K组;三个试验组的物种丰富度均高于对照组。从Shannon指数来看,对照组的菌群多样性高于试验组;三个试验组的菌群多样性以2组最高,以1组最低。拟杆菌门和厚壁菌门在1、2、3、K组的相对丰度分别为43.59%、55.33%、56.84%、56.01%和49.89%、40.32%、38.79%、39.95%,在各组中占比分别为93.48%、95.65%、95.63%、95.96%,是四组中的优势门,试验组和对照组中的相对丰度差异不明显。变形菌门在1、2、3组中的相对丰度(4.08%、1.41%、2.05%)高于对照组(0.97%),分别为对照组的4.2、1.45、2.11倍。互养菌门在1、2、3组中的相对丰度(0.72%、0.9%、0.49%)高于对照组(0.32%),分别为对照组的2.25、2.81、1.53倍。相对丰度前10位的属隶属于两个门(拟杆菌门和厚壁菌门)。多形杆状菌属在各组中的相对丰度分别为28.4%、29.4%、31.84%、28.23%,是四组中的优势属;其相对丰度在试验组和对照组中差异不明显。瘤胃球菌属和巨单胞菌属在1、2、3组中的相对丰度(8.03%、5.21%、3.97%和5.44%、2.05%、2.06%)高于对照组(2.44%和0.15%),分别是对照组的3.29、2.19、2.20倍和36.26、13.66、13.73倍。用发酵桂闽引象草替代部分日粮,鸡肠道菌群丰富度增高,多样性降低,对鸡肠道优势菌影响不明显,最主要的影响是变形菌门、互养菌门的数量增多及厚壁菌门中的瘤胃球菌属、巨单胞菌属的数量增多。
基金Supported by National Modern Agricultural Industry Technology System Guangxi Broiler Innovation Team Building Project(nycytxgxcxtd-19-01)Guangxi Agricultural Science and Technology Self-financing Project(Z2022114).
文摘[Objectives]To study the effects of Chinese herbal medicine feed additives on the growth performance and dietary nutrient metabolic rate of black-one chickens.[Methods]480 pieces of 1-day-old Donglan Black-bone Chickens were selected and divided into 4 groups,with 6 replicates in each group,with 20 pieces in each replicate.Among them,group A was the control group and was fed the basal diet;the groups B,C,and D were the experimental groups,which were separately supplemented with 0.25%,0.50%and 1.00%of Chinese herbal medicine additives in the basal diet,and the experimental period was 70 d.At the end of the feeding experiment,24 experimental chickens with similar body weight were selected from each group,and the same design of the feeding experiment was used to continue to feed the original diet for the metabolic experiment to determine the nutrient metabolic rate.[Results]Except the 50-70 d stage,the average daily gain(ADG)of group B in other stages was increased by 5.00%-9.15%(P<0.05),and the average body weight of group B at 70 d was also increased by 4.86%(P<0.05);the ADG of group C at 36-49,1-49 and 1-70 d and the average individual weight at 70 d were also higher than that of group A by 5.90%,2.66%,2.68%and 2.57%,respectively(P>0.05);at 36-49 d,the average daily feed intake(ADFI)of group A was higher than that of group B by 6.04%(P<0.01),and was also higher than that of groups C and D by 5.12%and 4.13%(P<0.05),respectively;at 1-49 d,the ADFI of group A was higher than that of group C by 2.86%(P<0.05),and there was no significant difference between the four groups of experimental chickens at each stage(P>0.05);at 22-35 and 1-70 d,the feed to gain ratio(F/G)of group B was 6.32%and 3.41%lower than that of group A(P<0.05),and at 36-49 and 1-49 d,the F/G of group B was even lower;at 36-49 d,the F/G of group C was 10.15%lower than that of group A(P<0.05),and at 1-49 d,it was also lower than that of group A by 5.29%(P<0.01);there was no significant difference between the two groups at other stages.In terms of nutrient metabolism:the three different amounts of this additive have increased the metabolic rate of DM,CP,CF,NFE,CA,Ca,P,TE and other nutrients in the diet to varying degrees,especially the addition of 0.25%and 0.50%had the significant increase.[Conclusions]The addition of 0.25%Chinese herbal medicine additives in the diet can significantly improve the growth performance and main nutrient metabolic rate of Donglan Black-bone Chickens.
