本文对6例乙型肝炎病人(4例急性肝炎,1例慢性迁延性肝炎,1例 HBsAg 阳性携带者)的血清进行了 Dane 抗体的研究。采用双抗体沉淀法,系将被检血清与氚标记 Dane 颗粒作用沉淀后,加入抗入 Ig 血清再沉淀,以上清液中残留放射性作为 Dane 抗...本文对6例乙型肝炎病人(4例急性肝炎,1例慢性迁延性肝炎,1例 HBsAg 阳性携带者)的血清进行了 Dane 抗体的研究。采用双抗体沉淀法,系将被检血清与氚标记 Dane 颗粒作用沉淀后,加入抗入 Ig 血清再沉淀,以上清液中残留放射性作为 Dane 抗体活性的指标。检测结果有2例急性乙肝病人血清中测出 Dane 抗体。这一抗体不同于抗-HBs、抗-HBc 或抗-HBe。根据临床随访,2例 Dane 抗体阳性者中一例恢复较快,并以后出现抗-HBs,另一例恢复不甚显著,血清中 HBsAg、HBeAg 均为阳性。作者认为进一步明确 Dane 抗体在清除血循环中乙肝病毒的作用,对研究慢性乙型肝炎发病机制有一定帮助.展开更多
New clerodane diterpenoids-tinocallone A, B, C, D and epitinopaylloloside(1-4.and 8) have been isolated from the root of Tinospora capillipes.Their structures were elucidated by chemical and spectral analysis.
The American Kennel Club and the Fédération Cynologique Internationale recognize two phenotypic variants of Great Dane breed denominated American and European varieties. Historically, these varieties have be...The American Kennel Club and the Fédération Cynologique Internationale recognize two phenotypic variants of Great Dane breed denominated American and European varieties. Historically, these varieties have been segregated according to morphological characteristics. In an attempt to obtain a better characterization, breeders have been interested in the genetic parameters that could evaluate the within-breed diversity. In this document, we studied the genetic structure of Great Dane breed with 10 STR markers in 88 dogs using capillary electrophoresis. Cluster analysis, population differentiation and phylogenetic analyses revealed that American and European varieties are genetically independent. Nevertheless, within the American variety a genetic stratification was found. Additionally, a high misclassification (28%) was detected, which could be due to wrong registration or false paternity. Our results support the importance to deem genetic markers as useful tools in breeding control. Similarly, these studies serve as an accurate reference to establish standards by dog breeding associations and for choosing among dogs for inter-breeding. Nevertheless, genetic tools are only a complement of morphological methods, since both are reshuffling the control over the breeding line. Notwithstanding, this database provides an overall and scape concerning the impact of genetic diversity within-breed. Unmistakably, more databases are needed to increase the quality of the breeding line as well as the number of STR in order to study, with more detail, the genetic structure in the Great Dane race.展开更多
文摘本文对6例乙型肝炎病人(4例急性肝炎,1例慢性迁延性肝炎,1例 HBsAg 阳性携带者)的血清进行了 Dane 抗体的研究。采用双抗体沉淀法,系将被检血清与氚标记 Dane 颗粒作用沉淀后,加入抗入 Ig 血清再沉淀,以上清液中残留放射性作为 Dane 抗体活性的指标。检测结果有2例急性乙肝病人血清中测出 Dane 抗体。这一抗体不同于抗-HBs、抗-HBc 或抗-HBe。根据临床随访,2例 Dane 抗体阳性者中一例恢复较快,并以后出现抗-HBs,另一例恢复不甚显著,血清中 HBsAg、HBeAg 均为阳性。作者认为进一步明确 Dane 抗体在清除血循环中乙肝病毒的作用,对研究慢性乙型肝炎发病机制有一定帮助.
文摘New clerodane diterpenoids-tinocallone A, B, C, D and epitinopaylloloside(1-4.and 8) have been isolated from the root of Tinospora capillipes.Their structures were elucidated by chemical and spectral analysis.
文摘The American Kennel Club and the Fédération Cynologique Internationale recognize two phenotypic variants of Great Dane breed denominated American and European varieties. Historically, these varieties have been segregated according to morphological characteristics. In an attempt to obtain a better characterization, breeders have been interested in the genetic parameters that could evaluate the within-breed diversity. In this document, we studied the genetic structure of Great Dane breed with 10 STR markers in 88 dogs using capillary electrophoresis. Cluster analysis, population differentiation and phylogenetic analyses revealed that American and European varieties are genetically independent. Nevertheless, within the American variety a genetic stratification was found. Additionally, a high misclassification (28%) was detected, which could be due to wrong registration or false paternity. Our results support the importance to deem genetic markers as useful tools in breeding control. Similarly, these studies serve as an accurate reference to establish standards by dog breeding associations and for choosing among dogs for inter-breeding. Nevertheless, genetic tools are only a complement of morphological methods, since both are reshuffling the control over the breeding line. Notwithstanding, this database provides an overall and scape concerning the impact of genetic diversity within-breed. Unmistakably, more databases are needed to increase the quality of the breeding line as well as the number of STR in order to study, with more detail, the genetic structure in the Great Dane race.
