Objective To explore the inhibitory effects of Nardostachys Jatamansi DC.volatile oil(NJVO)on psychological factors substance P(SP)/cortisol(CORT)-induced hyperpigmentation.Methods The model of psychologically-induced...Objective To explore the inhibitory effects of Nardostachys Jatamansi DC.volatile oil(NJVO)on psychological factors substance P(SP)/cortisol(CORT)-induced hyperpigmentation.Methods The model of psychologically-induced hyperpigmentation of B16F10 cells was created using SP(10 nmol/L)+CORT(10µmol/L)for 72 h.The levels of melanin content,tyrosinase(TYR)activity using NaOH lysis and L-dihydroxyphenylalanine(L-DOPA)oxidation methods were assessed,respectively.The effect of NJVO on SP/CORT-induced normal human skin tissue pigmentation was detected by Masson staining.Protein expressions of tyrosinase-related protein 1(TRP-1),tyrosinase-relative protein 2(DCT),and microphthalmia-associated transcription factor were determined using Western blot.The melanosome number,maturation,and melanosomal structure changes were detected through transmission electron microscopy and immunofluorescence experiments.In vivo,zebrafish pigment content was evaluated in SP/CORT-induced zebrafish hyperpigmentation model.Results NJVO significantly reduced the melanin content(P<0.01)and inhibited tyrosinase activity(P<0.01),the pigmentation of the normal skin tissue in the NJVO group was significantly lower than that in the SP/CORT group(P<0.05).And NJVO considerably downregulated expressions of melanogenesis-related proteins(TYR,TRP-1,DCT)in cells(P<0.01).In addition,the number of melanosomes was decreased and the dentrites formation of B16F10 cells was inhibited after NJVO treatment(P<0.01).In vivo,NJVO significantly reduced the pigment content in the zebrafish body(P<0.01).Conclusion NJVO effectively reversed SP/CORT-induced hyperpigmentation by suppressing the activity and expression of TYR and TRPs and inhibiting melanosome maturation in mouse B16F10 melanoma cells.展开更多
为实现南极磷虾(Euphausia superba)品质的快速评定,本研究将近红外光谱技术(Near infrared spectroscopy,NIRS)与偏最小二乘法(Partial least squares,PLS)相结合,构建用于快速预测磷虾体内非蛋白氮(Non-protein nitrogen,NPN)含量和...为实现南极磷虾(Euphausia superba)品质的快速评定,本研究将近红外光谱技术(Near infrared spectroscopy,NIRS)与偏最小二乘法(Partial least squares,PLS)相结合,构建用于快速预测磷虾体内非蛋白氮(Non-protein nitrogen,NPN)含量和挥发性盐基氮(Total volatile base nitrogen,TVB-N)含量的近红外定量分析模型。采集近红外光谱后,通过比较决定系数(Coefficient of determination,R^(2))、校正标准偏差(Root mean square error of calibration,RMSEC)、预测标准偏差(Root mean square error of prediction,RMSEP)等模型的评价参数,选取近红外光谱定量分析模型的最佳预处理方式、特征光谱范围以及主因子数。结果显示,NPN含量模型的最佳预处理方法为多元散射校正(Multiplicative signal correction,MSC),其特征光谱范围为8887.1~7774.2 cm-1;TVB-N含量模型则采用MSC与卷积平滑(Savitzky-Golay smoothing,SG)相结合的方式进行预处理,建模范围为全波段。两个定量模型的主因子数均为5。经模型的优化与外部验证,最终构建的PLS最优模型如下:NPN含量近红外定量分析模型的R^(2)为0.9384,RMSEC为0.279,RMSEP为0.443;TVB-N含量近红外定量分析模型的R^(2)为0.8685,RMSEC为3.800,RMSEP为4.070。研究结果表明,两个模型均具有良好的预测精度(R^(2)>0.85)和稳定性,其中NPN定量分析模型的预测能力优于TVB-N定量分析模型。综上,本研究基于NIRS与PLS构建的定量分析模型能够有效预测南极磷虾体内的NPN和TVB-N含量,为南极磷虾的品质评价提供了可靠的技术支持,满足快速评定的实际应用需求。展开更多
以分别来自巴布亚·新几内亚(Papua New Guinea)、厄瓜多尔(Ecuador)和加纳(Ghana)产地的3种可可豆和3种可可液块制成的6组100%黑巧克力作为研究对象。将超气相电子鼻与感官评价相结合,采用主成分分析和正交偏最小二乘判别分析对其...以分别来自巴布亚·新几内亚(Papua New Guinea)、厄瓜多尔(Ecuador)和加纳(Ghana)产地的3种可可豆和3种可可液块制成的6组100%黑巧克力作为研究对象。将超气相电子鼻与感官评价相结合,采用主成分分析和正交偏最小二乘判别分析对其风味进行解析,探索不同产地可可豆和可可液块制成的100%黑巧克力的风味差异。6款样品中共检测出30种挥发性风味化合物,基于预测变量重要性投影(variable importance in projection,VIP)值进一步筛选出醛类、酮类、吡嗪类等22种主要差异挥发性风味化合物。主成分分析与正交偏最小二乘判别分析结果相一致,3种可可豆制备的100%豆到块(bean to bar,BTB)黑巧克力与3款可可液块制备的100%黑巧克力风味得到明显区分,模型拟合效果好,验证有效,分析结果可靠。可可豆制备的100%BTB黑巧克力样品果香、烘烤香、可可香、坚果香会更浓郁,果味、酸味、巧克力味会更突显。综上,超快速气相电子鼻结合多元统计,可对不同产地可可豆和可可液块制备的100%黑巧克力风味进行有效快速区分,可可豆制备的100%BTB黑巧克力的挥发性风味化合物总含量和风味强度相比同产地的可可液块制备的100%黑巧克力表现更高,感官评价更突出,风味更愉悦。展开更多
基金Supported by the Open Project of National Major Science and Technology Infrastructure of Translational Medicine,China(No.TMSK-2021-404)。
文摘Objective To explore the inhibitory effects of Nardostachys Jatamansi DC.volatile oil(NJVO)on psychological factors substance P(SP)/cortisol(CORT)-induced hyperpigmentation.Methods The model of psychologically-induced hyperpigmentation of B16F10 cells was created using SP(10 nmol/L)+CORT(10µmol/L)for 72 h.The levels of melanin content,tyrosinase(TYR)activity using NaOH lysis and L-dihydroxyphenylalanine(L-DOPA)oxidation methods were assessed,respectively.The effect of NJVO on SP/CORT-induced normal human skin tissue pigmentation was detected by Masson staining.Protein expressions of tyrosinase-related protein 1(TRP-1),tyrosinase-relative protein 2(DCT),and microphthalmia-associated transcription factor were determined using Western blot.The melanosome number,maturation,and melanosomal structure changes were detected through transmission electron microscopy and immunofluorescence experiments.In vivo,zebrafish pigment content was evaluated in SP/CORT-induced zebrafish hyperpigmentation model.Results NJVO significantly reduced the melanin content(P<0.01)and inhibited tyrosinase activity(P<0.01),the pigmentation of the normal skin tissue in the NJVO group was significantly lower than that in the SP/CORT group(P<0.05).And NJVO considerably downregulated expressions of melanogenesis-related proteins(TYR,TRP-1,DCT)in cells(P<0.01).In addition,the number of melanosomes was decreased and the dentrites formation of B16F10 cells was inhibited after NJVO treatment(P<0.01).In vivo,NJVO significantly reduced the pigment content in the zebrafish body(P<0.01).Conclusion NJVO effectively reversed SP/CORT-induced hyperpigmentation by suppressing the activity and expression of TYR and TRPs and inhibiting melanosome maturation in mouse B16F10 melanoma cells.
文摘为实现南极磷虾(Euphausia superba)品质的快速评定,本研究将近红外光谱技术(Near infrared spectroscopy,NIRS)与偏最小二乘法(Partial least squares,PLS)相结合,构建用于快速预测磷虾体内非蛋白氮(Non-protein nitrogen,NPN)含量和挥发性盐基氮(Total volatile base nitrogen,TVB-N)含量的近红外定量分析模型。采集近红外光谱后,通过比较决定系数(Coefficient of determination,R^(2))、校正标准偏差(Root mean square error of calibration,RMSEC)、预测标准偏差(Root mean square error of prediction,RMSEP)等模型的评价参数,选取近红外光谱定量分析模型的最佳预处理方式、特征光谱范围以及主因子数。结果显示,NPN含量模型的最佳预处理方法为多元散射校正(Multiplicative signal correction,MSC),其特征光谱范围为8887.1~7774.2 cm-1;TVB-N含量模型则采用MSC与卷积平滑(Savitzky-Golay smoothing,SG)相结合的方式进行预处理,建模范围为全波段。两个定量模型的主因子数均为5。经模型的优化与外部验证,最终构建的PLS最优模型如下:NPN含量近红外定量分析模型的R^(2)为0.9384,RMSEC为0.279,RMSEP为0.443;TVB-N含量近红外定量分析模型的R^(2)为0.8685,RMSEC为3.800,RMSEP为4.070。研究结果表明,两个模型均具有良好的预测精度(R^(2)>0.85)和稳定性,其中NPN定量分析模型的预测能力优于TVB-N定量分析模型。综上,本研究基于NIRS与PLS构建的定量分析模型能够有效预测南极磷虾体内的NPN和TVB-N含量,为南极磷虾的品质评价提供了可靠的技术支持,满足快速评定的实际应用需求。
文摘以分别来自巴布亚·新几内亚(Papua New Guinea)、厄瓜多尔(Ecuador)和加纳(Ghana)产地的3种可可豆和3种可可液块制成的6组100%黑巧克力作为研究对象。将超气相电子鼻与感官评价相结合,采用主成分分析和正交偏最小二乘判别分析对其风味进行解析,探索不同产地可可豆和可可液块制成的100%黑巧克力的风味差异。6款样品中共检测出30种挥发性风味化合物,基于预测变量重要性投影(variable importance in projection,VIP)值进一步筛选出醛类、酮类、吡嗪类等22种主要差异挥发性风味化合物。主成分分析与正交偏最小二乘判别分析结果相一致,3种可可豆制备的100%豆到块(bean to bar,BTB)黑巧克力与3款可可液块制备的100%黑巧克力风味得到明显区分,模型拟合效果好,验证有效,分析结果可靠。可可豆制备的100%BTB黑巧克力样品果香、烘烤香、可可香、坚果香会更浓郁,果味、酸味、巧克力味会更突显。综上,超快速气相电子鼻结合多元统计,可对不同产地可可豆和可可液块制备的100%黑巧克力风味进行有效快速区分,可可豆制备的100%BTB黑巧克力的挥发性风味化合物总含量和风味强度相比同产地的可可液块制备的100%黑巧克力表现更高,感官评价更突出,风味更愉悦。
