Objective To explore the inhibitory effects of Nardostachys Jatamansi DC.volatile oil(NJVO)on psychological factors substance P(SP)/cortisol(CORT)-induced hyperpigmentation.Methods The model of psychologically-induced...Objective To explore the inhibitory effects of Nardostachys Jatamansi DC.volatile oil(NJVO)on psychological factors substance P(SP)/cortisol(CORT)-induced hyperpigmentation.Methods The model of psychologically-induced hyperpigmentation of B16F10 cells was created using SP(10 nmol/L)+CORT(10µmol/L)for 72 h.The levels of melanin content,tyrosinase(TYR)activity using NaOH lysis and L-dihydroxyphenylalanine(L-DOPA)oxidation methods were assessed,respectively.The effect of NJVO on SP/CORT-induced normal human skin tissue pigmentation was detected by Masson staining.Protein expressions of tyrosinase-related protein 1(TRP-1),tyrosinase-relative protein 2(DCT),and microphthalmia-associated transcription factor were determined using Western blot.The melanosome number,maturation,and melanosomal structure changes were detected through transmission electron microscopy and immunofluorescence experiments.In vivo,zebrafish pigment content was evaluated in SP/CORT-induced zebrafish hyperpigmentation model.Results NJVO significantly reduced the melanin content(P<0.01)and inhibited tyrosinase activity(P<0.01),the pigmentation of the normal skin tissue in the NJVO group was significantly lower than that in the SP/CORT group(P<0.05).And NJVO considerably downregulated expressions of melanogenesis-related proteins(TYR,TRP-1,DCT)in cells(P<0.01).In addition,the number of melanosomes was decreased and the dentrites formation of B16F10 cells was inhibited after NJVO treatment(P<0.01).In vivo,NJVO significantly reduced the pigment content in the zebrafish body(P<0.01).Conclusion NJVO effectively reversed SP/CORT-induced hyperpigmentation by suppressing the activity and expression of TYR and TRPs and inhibiting melanosome maturation in mouse B16F10 melanoma cells.展开更多
为鉴定线香中主要挥发性成分,进一步揭示这些成分在不同样品间的差异及其对香气特征的贡献,采用顶空固相微萃取-气相色谱-质谱联用技术(headspace solid-phase microextraction gas chromatography-mass spectrometry,HS-SPME-GC-MS)结...为鉴定线香中主要挥发性成分,进一步揭示这些成分在不同样品间的差异及其对香气特征的贡献,采用顶空固相微萃取-气相色谱-质谱联用技术(headspace solid-phase microextraction gas chromatography-mass spectrometry,HS-SPME-GC-MS)结合多元统计分析方法对粘粉燃烧产物、金银花粉、金银花粉燃烧产物、线香燃烧产物中挥发性成分进行提取鉴定,并确定关键挥发性成分。结果表明,从4种样品中共鉴定出102种挥发性成分,以芳香族、杂环类、酮类化合物等成分为主。主成分分析、正交偏最小二乘法判别分析及聚类热图分析表明,金银花粉燃烧产物和线香燃烧产物挥发性成分组成相似,与粘粉燃烧产物及金银花粉挥发性成分存在较大差异,并筛选出25种投影变量的重要性(variable important for the projection,VIP)>1的关键挥发性成分。研究结果为金银花线香的进一步开发提供理论依据,也为中药材的应用拓展了新方向。展开更多
基金Supported by the Open Project of National Major Science and Technology Infrastructure of Translational Medicine,China(No.TMSK-2021-404)。
文摘Objective To explore the inhibitory effects of Nardostachys Jatamansi DC.volatile oil(NJVO)on psychological factors substance P(SP)/cortisol(CORT)-induced hyperpigmentation.Methods The model of psychologically-induced hyperpigmentation of B16F10 cells was created using SP(10 nmol/L)+CORT(10µmol/L)for 72 h.The levels of melanin content,tyrosinase(TYR)activity using NaOH lysis and L-dihydroxyphenylalanine(L-DOPA)oxidation methods were assessed,respectively.The effect of NJVO on SP/CORT-induced normal human skin tissue pigmentation was detected by Masson staining.Protein expressions of tyrosinase-related protein 1(TRP-1),tyrosinase-relative protein 2(DCT),and microphthalmia-associated transcription factor were determined using Western blot.The melanosome number,maturation,and melanosomal structure changes were detected through transmission electron microscopy and immunofluorescence experiments.In vivo,zebrafish pigment content was evaluated in SP/CORT-induced zebrafish hyperpigmentation model.Results NJVO significantly reduced the melanin content(P<0.01)and inhibited tyrosinase activity(P<0.01),the pigmentation of the normal skin tissue in the NJVO group was significantly lower than that in the SP/CORT group(P<0.05).And NJVO considerably downregulated expressions of melanogenesis-related proteins(TYR,TRP-1,DCT)in cells(P<0.01).In addition,the number of melanosomes was decreased and the dentrites formation of B16F10 cells was inhibited after NJVO treatment(P<0.01).In vivo,NJVO significantly reduced the pigment content in the zebrafish body(P<0.01).Conclusion NJVO effectively reversed SP/CORT-induced hyperpigmentation by suppressing the activity and expression of TYR and TRPs and inhibiting melanosome maturation in mouse B16F10 melanoma cells.
文摘为鉴定线香中主要挥发性成分,进一步揭示这些成分在不同样品间的差异及其对香气特征的贡献,采用顶空固相微萃取-气相色谱-质谱联用技术(headspace solid-phase microextraction gas chromatography-mass spectrometry,HS-SPME-GC-MS)结合多元统计分析方法对粘粉燃烧产物、金银花粉、金银花粉燃烧产物、线香燃烧产物中挥发性成分进行提取鉴定,并确定关键挥发性成分。结果表明,从4种样品中共鉴定出102种挥发性成分,以芳香族、杂环类、酮类化合物等成分为主。主成分分析、正交偏最小二乘法判别分析及聚类热图分析表明,金银花粉燃烧产物和线香燃烧产物挥发性成分组成相似,与粘粉燃烧产物及金银花粉挥发性成分存在较大差异,并筛选出25种投影变量的重要性(variable important for the projection,VIP)>1的关键挥发性成分。研究结果为金银花线香的进一步开发提供理论依据,也为中药材的应用拓展了新方向。
