Sand dabs, an important commercial flatfish in the Bohai Sea, has recently decreased gradually in biomass, resulting from overfishing and marine pollution. Artificial culture and multiplication of sand dabs are essent...Sand dabs, an important commercial flatfish in the Bohai Sea, has recently decreased gradually in biomass, resulting from overfishing and marine pollution. Artificial culture and multiplication of sand dabs are essential and practical in China. The present paper examines the food habitof this fish to provide detailed information for its culture and multiplication.展开更多
Hyperglycemia and bacterial colonization in diabetic wounds aberrantly activate Nod-like receptor protein 3(NLRP3)in macrophages,resulting in extensive inflammatory infiltration and impaired wound healing.Targeted sup...Hyperglycemia and bacterial colonization in diabetic wounds aberrantly activate Nod-like receptor protein 3(NLRP3)in macrophages,resulting in extensive inflammatory infiltration and impaired wound healing.Targeted suppression of the NLRP3 inflammasome shows promise in reducing macrophage inflammatory disruptions.However,challenges such as drug off-target effects and degradation via lysosomal capture remain during treatment.In this study,engineered apoptotic bodies(BHB-dABs)derived from adipose stem cells loaded with β-hydroxybutyric acid(BHB)were synthesized via biosynthesis.These vesicles target M1-type macrophages,which highly express the folic acid receptor in the inflammatory microenvironment,and facilitate lysosomal escape through 1,2-distearoyl-sn-propyltriyl-3-phosphatidylethanolaminepolyethylene glycol functionalization,which may enhance the efficacy of NLRP3 inhibition for managing diabetic wounds.Invitro studies demonstrated the biocompatibility of BHB-dABs,their selective targeting of M1-type macrophages,and their ability to release BHB within the inflammatory microenvironment via folic acid and folic acid receptor signaling.These nanovesicles exhibited lysosomal escape,antiinflammatory,mitochondrial protection,and endothelial cell vascularization properties.Invivo experiments demonstrated that BHB-dABs enhance the recovery of diabetic wound inflammation and angiogenesis,accelerating wound healing.These functionalized apoptotic bodies efficiently deliver NLRP3 inflammasome inhibitors using a dual strategy of targeting macrophages and promoting lysosomal escape.This approach represents a novel therapeutic strategy for effectively treating chronic diabetic wounds.展开更多
文摘Sand dabs, an important commercial flatfish in the Bohai Sea, has recently decreased gradually in biomass, resulting from overfishing and marine pollution. Artificial culture and multiplication of sand dabs are essential and practical in China. The present paper examines the food habitof this fish to provide detailed information for its culture and multiplication.
基金supported by grants from the National Key Research and Development Program of China(2020YFA0908200)the National Natural Science Foundation of China(82072177,82272263,and 82272264)+2 种基金the“Two Hundred Talent”Program and the first Continuous Outstanding Special Technology Supporting Project and Shanghai Municipal Key Clinical Specialty(shslczdzk00901)the Shanghai“Rising Stars of Medical Talents”Youth Development Program,Shanghai Clinical Research Center of Plastic and Reconstructive Surgery supported by Science and Technology Commission of Shanghai Municipality(grant 22MC1940300)the Science and Technology Commission of Shanghai Municipality(23015820800).
文摘Hyperglycemia and bacterial colonization in diabetic wounds aberrantly activate Nod-like receptor protein 3(NLRP3)in macrophages,resulting in extensive inflammatory infiltration and impaired wound healing.Targeted suppression of the NLRP3 inflammasome shows promise in reducing macrophage inflammatory disruptions.However,challenges such as drug off-target effects and degradation via lysosomal capture remain during treatment.In this study,engineered apoptotic bodies(BHB-dABs)derived from adipose stem cells loaded with β-hydroxybutyric acid(BHB)were synthesized via biosynthesis.These vesicles target M1-type macrophages,which highly express the folic acid receptor in the inflammatory microenvironment,and facilitate lysosomal escape through 1,2-distearoyl-sn-propyltriyl-3-phosphatidylethanolaminepolyethylene glycol functionalization,which may enhance the efficacy of NLRP3 inhibition for managing diabetic wounds.Invitro studies demonstrated the biocompatibility of BHB-dABs,their selective targeting of M1-type macrophages,and their ability to release BHB within the inflammatory microenvironment via folic acid and folic acid receptor signaling.These nanovesicles exhibited lysosomal escape,antiinflammatory,mitochondrial protection,and endothelial cell vascularization properties.Invivo experiments demonstrated that BHB-dABs enhance the recovery of diabetic wound inflammation and angiogenesis,accelerating wound healing.These functionalized apoptotic bodies efficiently deliver NLRP3 inflammasome inhibitors using a dual strategy of targeting macrophages and promoting lysosomal escape.This approach represents a novel therapeutic strategy for effectively treating chronic diabetic wounds.
