AIM To reveal the inhibitory effects ofCurcuma aromatica oil(CAO)on cellproliferation of hepatoma in mice.METHODS Two tumor inhibitory experimentsof CAO on hepatoma in mice were conducted.The inhibitory effects of CAO...AIM To reveal the inhibitory effects ofCurcuma aromatica oil(CAO)on cellproliferation of hepatoma in mice.METHODS Two tumor inhibitory experimentsof CAO on hepatoma in mice were conducted.The inhibitory effects of CAO on proliferation ofhepatoma in mice were evaluated by DNA imagecytometry and immunohistochemical staining ofproliferating cell nuclear antigen(PCNA).RESULTS The tumor inhibitory rates of CAOwere 52% and 51% in two experiments,respectively.Compared with those of the saline-treated control groups,both differences werestatistically significant(P【0.01).In the groupof mice treated with CAO,the cellular nuclearDNA OD value(249±70),areas(623 μm^2±228 μm^2)and DNA(2.38±0.67)index of hepaticcarcinomas were significantly lower than thoseof the control group(430±160,1073 μm^2±101 μm^2 and 4.48±0.71).CAO also couldincrease diploidy cell rates(29.00%±9.34% vs2.97%±5.69%,P【0.01)and decreasepentaploidy cell exceeding rate(30.04%±15.10% vs 70.89%±14.94%,P【0.01).In thegroup of mice treated with CAO,the labelingindexes of proliferating cell nuclear antigen (PCNA-LI)were 30%±4%,which weresignificantly lower than 40%±6% of the controlgroup(P【0.01).CONCLUSION The inhibition of CAO on thegrowth of hepatoma in mice might be associatedwith its depression on cellular proliferativeactivity.展开更多
本研究收集了10份姜黄属(Curcuma)种质资源,应用32对SSR标记对其进行了遗传多样性和系统发育分析,一共检测到116个多态性位点,引物多态信息含量(polymorphism information content,PIC)平均值为0.6143,观测杂合度(observed heterozygosi...本研究收集了10份姜黄属(Curcuma)种质资源,应用32对SSR标记对其进行了遗传多样性和系统发育分析,一共检测到116个多态性位点,引物多态信息含量(polymorphism information content,PIC)平均值为0.6143,观测杂合度(observed heterozygosity,Ho)平均值为0.5102,期望杂合度(expected heterozygosity,He)平均值为0.6432。Shannon遗传多样性指数(Shannon's information index,I)平均值为1.0802;Nei's遗传多样性指数(Nei's genetic diversity index,Nei)平均值为0.6008,平均遗传分化系数(genetic differentiation,Fst)为0.1886。通过亲缘关系分析与主坐标分析,10份姜黄属资源分为2个亚群,亚群间基因流(gene flow,Nm)为1.0752。结果表明,10份姜黄属种质资源具有较高的遗传多样性,本研究有利于姜黄属植物的鉴定及遗传研究。展开更多
基金National key project of the 9~(th) 5-year Plan for Medicine and Health,No.96-906-07-04Guangdong provincial natural scientific grants,No.980663.
文摘AIM To reveal the inhibitory effects ofCurcuma aromatica oil(CAO)on cellproliferation of hepatoma in mice.METHODS Two tumor inhibitory experimentsof CAO on hepatoma in mice were conducted.The inhibitory effects of CAO on proliferation ofhepatoma in mice were evaluated by DNA imagecytometry and immunohistochemical staining ofproliferating cell nuclear antigen(PCNA).RESULTS The tumor inhibitory rates of CAOwere 52% and 51% in two experiments,respectively.Compared with those of the saline-treated control groups,both differences werestatistically significant(P【0.01).In the groupof mice treated with CAO,the cellular nuclearDNA OD value(249±70),areas(623 μm^2±228 μm^2)and DNA(2.38±0.67)index of hepaticcarcinomas were significantly lower than thoseof the control group(430±160,1073 μm^2±101 μm^2 and 4.48±0.71).CAO also couldincrease diploidy cell rates(29.00%±9.34% vs2.97%±5.69%,P【0.01)and decreasepentaploidy cell exceeding rate(30.04%±15.10% vs 70.89%±14.94%,P【0.01).In thegroup of mice treated with CAO,the labelingindexes of proliferating cell nuclear antigen (PCNA-LI)were 30%±4%,which weresignificantly lower than 40%±6% of the controlgroup(P【0.01).CONCLUSION The inhibition of CAO on thegrowth of hepatoma in mice might be associatedwith its depression on cellular proliferativeactivity.
