Geographic isolation is a key factor in shaping the genetic structure of many fish species.Two sympatric species,Culter alburnus and C.mongolicus,are economically important fish that are widely distributed in China an...Geographic isolation is a key factor in shaping the genetic structure of many fish species.Two sympatric species,Culter alburnus and C.mongolicus,are economically important fish that are widely distributed in China and have been recently used as new aquaculture species.We used the mitochondrial DNA control region(CR)as a marker to investigate the genetic structure of the two species.Phylogenetic analysis revealed two major lineages(Lineages I and II)that were highly consistent with geographical patterns for C.alburnus and C.mongolicus.Based on genetic distance,the Zhujiang(Pearl)River Basin(ZRB)populations potentially represented a cryptic subspecies,which might be differentiated as the result of strict geographic isolation,an earlier diverged event,or peripheral areas.Genetic diversity analysis suggested that the Changjiang(Yangtze)River Basin(CRB)populations are located at,or near the core region of their origination,not only due to the larger population size at the CRB,but also their habitat diversity and suitability for its survival,and the genetic diversity dif ferences among basin populations were significant for the two species.Moreover,demographic analysis indicated that the two Culter species and most populations had undergone a period of population expansion during warm interglacial periods.However,the C.alburnus population of Huaihe River Basin(Weishan Lake)exhibited different patterns during the interglacial period,which may due to the latest diverged time of HRB and Weishan Lake located at the permafrost.Notably,the ZRB(in Songtao Reservoir)C.mongolicus population showed no genetic diversity and had a unique haplotype,which could be treated as a special gene pool for species conservation.In summary,geographic isolation is most likely responsible for the two Culter species distribution patterns.展开更多
[ Objective ] This study aimed to provide references for reasonable drug administration in Culter mongolicus and promote the development of its breeding industry. [Method] C. mongolicus was administrated with cypermet...[ Objective ] This study aimed to provide references for reasonable drug administration in Culter mongolicus and promote the development of its breeding industry. [Method] C. mongolicus was administrated with cypermethrin, mebendazole, trichlorfon, cupric sulfate, trichloroisocyanuric acid, povidone iodine and phoxim, respectively, to analyze its susceptibility to different drugs. [ Result] C. mongolicus was very sensitive to cypermethrin; the median tolerance limit （TLM） at 3,8 h was 0. 072mg/L, minimum lethal concentration was 0. 063 - 0. 069 mg,/L, safe concentration was 0. 021 6 mg/L. C. mongolicus was insensitive to mebendazole, trichlorfon, cupric sulfate, trichloroisocyanuric acid and povidone iodine. According to safe concentration of various drugs, C. mongolicus exhibited higher susceptibility to cypermethrin than to phoxim. [ Conclusion] This study laid a solid foundation for better protection and development of C. mongolicus resources.展开更多
Research was conducted on topmouth culter (Culter alburnus) grown in ponds and lakes as well as wild types in order to determine their chemical composition and nutritional value. There are three types of fish that dif...Research was conducted on topmouth culter (Culter alburnus) grown in ponds and lakes as well as wild types in order to determine their chemical composition and nutritional value. There are three types of fish that differ in their proximate composition, amino acids, fatty acids, and minerals. Wild fish had a significantly lower crude lipid contents than cultured fish (P P ∑PUFAs) showed an obviously opposite trend. As compared with cultured fish, wild fish had significantly higher levels of n-3 PUFAs, arachidonic acid (AA), eicosapentaenoic acid (EPA) and docosahxaenoic acid (DHA) (P P > 0.05), except for Na, Fe and Se. In conclusion, diet composition and external aqueous environment may determine the differences between wild and cultured topmouth culter.展开更多
Myostatin is a member of the TGF-βsuperfamily and functions as a negative regulator for skeletal muscle development and growth.It has become the most targeted gene in aquaculture that used for selective breeding.Prev...Myostatin is a member of the TGF-βsuperfamily and functions as a negative regulator for skeletal muscle development and growth.It has become the most targeted gene in aquaculture that used for selective breeding.Previous studies involved in genome editing in several fish species confirmed that CRISPR/Cas9(Clustered Regularly Interspaced Short Palindromic Repeats)system was highly efficient with lower off-target effect,however,no reports were raised in Culter alburnus.In this study,we employed CRISPR/Cas9 gene editing system to successfully disrupt mstn gene by co-injection with Cas9 protein and the targeted sgRNA in C.alburnus.Various Indel mutations were obtained with 82% knockout efficiency in the F0 generation by PCR sequencing.In addition,mutations in mstn that induced by CRISPR/Cas9 were detected in the F1 generation by individually mating the wild-type female with the F0 generation of mstn-KO male at sexual maturity.More importantly,the body weight and length were significantly elevated in mstn±group when compared to those of the control.As expected in mstn±group,the expression level of mstn was sharply reduced,whereas a slight increase was observed in two growth-related genes(myod and myog).Moreover,higher numbers of muscle fibers were observed in mstn±group,meaning that growth performance in mstn±individuals might be represented by increasing the number of muscle fibers.Taken together,our current study successfully obtained a site-specific modification of mstn using CRISPR/Cas9 technology,and these results provided a new insight for facilitating topmouth culter genetic studies and breeding.展开更多
The topmouth culter(Culter alburnus)is at the top of the food chain and plays an important role in the maintenance of freshwater ecosystem stability.It is also a commercially important aquaculture fish species in Chin...The topmouth culter(Culter alburnus)is at the top of the food chain and plays an important role in the maintenance of freshwater ecosystem stability.It is also a commercially important aquaculture fish species in China.To establish conservation strategies and breeding programs,a total of 16 polymorphic microsatellite loci were isolated from transcriptome-derived sequences of C.alburnus.Characterization of these loci was assessed against 1 wild and 3 cultured populations in Huzhou,China.The number of alleles per locus ranged from 3 to 10 with an average of 5.25.A bottleneck effect was observed in all 4 populations;The ChangXing population showed moderate genetic differentiation when compared with other populations(FST values,0.0695-0.0788).The microsatellite loci would be useful for further studies on population genetic diversity,genetic structure,and assessments of the artificial propagation release effect of C.alburnus.展开更多
基金Supported by the National Key Technology Research and Development Program(No.2015BAD25B01)the Earmarked Fund for China Agriculture Research system(No.CARS-45)
文摘Geographic isolation is a key factor in shaping the genetic structure of many fish species.Two sympatric species,Culter alburnus and C.mongolicus,are economically important fish that are widely distributed in China and have been recently used as new aquaculture species.We used the mitochondrial DNA control region(CR)as a marker to investigate the genetic structure of the two species.Phylogenetic analysis revealed two major lineages(Lineages I and II)that were highly consistent with geographical patterns for C.alburnus and C.mongolicus.Based on genetic distance,the Zhujiang(Pearl)River Basin(ZRB)populations potentially represented a cryptic subspecies,which might be differentiated as the result of strict geographic isolation,an earlier diverged event,or peripheral areas.Genetic diversity analysis suggested that the Changjiang(Yangtze)River Basin(CRB)populations are located at,or near the core region of their origination,not only due to the larger population size at the CRB,but also their habitat diversity and suitability for its survival,and the genetic diversity dif ferences among basin populations were significant for the two species.Moreover,demographic analysis indicated that the two Culter species and most populations had undergone a period of population expansion during warm interglacial periods.However,the C.alburnus population of Huaihe River Basin(Weishan Lake)exhibited different patterns during the interglacial period,which may due to the latest diverged time of HRB and Weishan Lake located at the permafrost.Notably,the ZRB(in Songtao Reservoir)C.mongolicus population showed no genetic diversity and had a unique haplotype,which could be treated as a special gene pool for species conservation.In summary,geographic isolation is most likely responsible for the two Culter species distribution patterns.
基金Supported by Science and Technology Project of Jiangxi Province(20132BBF60051)
文摘[ Objective ] This study aimed to provide references for reasonable drug administration in Culter mongolicus and promote the development of its breeding industry. [Method] C. mongolicus was administrated with cypermethrin, mebendazole, trichlorfon, cupric sulfate, trichloroisocyanuric acid, povidone iodine and phoxim, respectively, to analyze its susceptibility to different drugs. [ Result] C. mongolicus was very sensitive to cypermethrin; the median tolerance limit （TLM） at 3,8 h was 0. 072mg/L, minimum lethal concentration was 0. 063 - 0. 069 mg,/L, safe concentration was 0. 021 6 mg/L. C. mongolicus was insensitive to mebendazole, trichlorfon, cupric sulfate, trichloroisocyanuric acid and povidone iodine. According to safe concentration of various drugs, C. mongolicus exhibited higher susceptibility to cypermethrin than to phoxim. [ Conclusion] This study laid a solid foundation for better protection and development of C. mongolicus resources.
文摘Research was conducted on topmouth culter (Culter alburnus) grown in ponds and lakes as well as wild types in order to determine their chemical composition and nutritional value. There are three types of fish that differ in their proximate composition, amino acids, fatty acids, and minerals. Wild fish had a significantly lower crude lipid contents than cultured fish (P P ∑PUFAs) showed an obviously opposite trend. As compared with cultured fish, wild fish had significantly higher levels of n-3 PUFAs, arachidonic acid (AA), eicosapentaenoic acid (EPA) and docosahxaenoic acid (DHA) (P P > 0.05), except for Na, Fe and Se. In conclusion, diet composition and external aqueous environment may determine the differences between wild and cultured topmouth culter.
基金supported by Zhejiang Provincial Natural Science Foundation of China(LTGN23C190008)Key Scientific and Technological Grant of Zhejiang for Breeding New Agricultural Varieties(2021C02069-3).
文摘Myostatin is a member of the TGF-βsuperfamily and functions as a negative regulator for skeletal muscle development and growth.It has become the most targeted gene in aquaculture that used for selective breeding.Previous studies involved in genome editing in several fish species confirmed that CRISPR/Cas9(Clustered Regularly Interspaced Short Palindromic Repeats)system was highly efficient with lower off-target effect,however,no reports were raised in Culter alburnus.In this study,we employed CRISPR/Cas9 gene editing system to successfully disrupt mstn gene by co-injection with Cas9 protein and the targeted sgRNA in C.alburnus.Various Indel mutations were obtained with 82% knockout efficiency in the F0 generation by PCR sequencing.In addition,mutations in mstn that induced by CRISPR/Cas9 were detected in the F1 generation by individually mating the wild-type female with the F0 generation of mstn-KO male at sexual maturity.More importantly,the body weight and length were significantly elevated in mstn±group when compared to those of the control.As expected in mstn±group,the expression level of mstn was sharply reduced,whereas a slight increase was observed in two growth-related genes(myod and myog).Moreover,higher numbers of muscle fibers were observed in mstn±group,meaning that growth performance in mstn±individuals might be represented by increasing the number of muscle fibers.Taken together,our current study successfully obtained a site-specific modification of mstn using CRISPR/Cas9 technology,and these results provided a new insight for facilitating topmouth culter genetic studies and breeding.
基金supported by the Major science and technology programs for agriculture (aquaculture)new variety breeding in Zhejiang province (2021C02069-3).
文摘The topmouth culter(Culter alburnus)is at the top of the food chain and plays an important role in the maintenance of freshwater ecosystem stability.It is also a commercially important aquaculture fish species in China.To establish conservation strategies and breeding programs,a total of 16 polymorphic microsatellite loci were isolated from transcriptome-derived sequences of C.alburnus.Characterization of these loci was assessed against 1 wild and 3 cultured populations in Huzhou,China.The number of alleles per locus ranged from 3 to 10 with an average of 5.25.A bottleneck effect was observed in all 4 populations;The ChangXing population showed moderate genetic differentiation when compared with other populations(FST values,0.0695-0.0788).The microsatellite loci would be useful for further studies on population genetic diversity,genetic structure,and assessments of the artificial propagation release effect of C.alburnus.
