In order to optimize the microwave-assisted extraction technology of polyphenols from Loropetalum chinense （R. Br.） Oliv., the effects of microwave power, ethanol concentration, solid to liquid ratio and extraction ...In order to optimize the microwave-assisted extraction technology of polyphenols from Loropetalum chinense （R. Br.） Oliv., the effects of microwave power, ethanol concentration, solid to liquid ratio and extraction time on polyphenols extraction rate were investigated. On the basis of single-factor test, a four-factor and three-level orthogonal test was designed by response surface method to establish a mathematical model between the response value and various factors. The results showed that the intensity of effect of different influencing factor on polyphenols ex- traction rate ranked as microwave power＇s〉solid to liquid ratio＇s〉extraction time＇s〉 ethanol concentration＇s. The optimum microwave-assisted extraction conditions for polyphenols from L. chinense were as follows： extraction power 254 W, ethanol concentration 60%, extraction time 12.5 rain and solid to liquid ratio 1：17. Under the optimum extraction conditions, the extraction rate of polyphenols from L. chinense was 19.17%.展开更多
A reliable ultrasound-assisted extraction （UAE） method combined with HPLC-UV for quantification of eight active alkaloids in fruits of Macleaya cordata （Willd） R. Br. was developed. The optimization conditions of ...A reliable ultrasound-assisted extraction （UAE） method combined with HPLC-UV for quantification of eight active alkaloids in fruits of Macleaya cordata （Willd） R. Br. was developed. The optimization conditions of UAE were obtained by using Box-Behnken design of response surface methodology. Chromatography was carried out using a Kromasil C18 column by gradient elution with 0.1% phosphoric acid aqueous solution for HPLC-UV. All calibration curves showed good linear correlation coefficients （R^2〉0.999 6） and recoveries （from 97.3% to 104.9%） were acceptable. 1,1-diphenyl-2-picrylhydrazyl （DPPH） method was employed to test the antioxidant activity of the extract from the samples. The proposed method was successfully applied to quantifying eight components in nine samples of M.cordata, and significant variations of alkaloid contents and antioxidant aetivity of the samples from different habitats were demonstrated. It presents a powerful proof for the selection of the best sources to extract eight kinds of alkaloids.展开更多
[Objectives]To explore the stability of sanguinarine and chelerythrine in Macleaya cordata(Willd.)R.Br.[Methods]The solubility and stability of sanguinarine and chelerythrine in seven solvents were measured by HPLC.Be...[Objectives]To explore the stability of sanguinarine and chelerythrine in Macleaya cordata(Willd.)R.Br.[Methods]The solubility and stability of sanguinarine and chelerythrine in seven solvents were measured by HPLC.Besides,the effects of water quality,light source,oxidant,temperature,and pH on stability were investigated.[Results]The solubility and stability of sanguinarine and chelerythrine in methanol and ethanol are good;the stability of sanguinarine and chelerythrine in distilled water and rainwater is not affected by light and is very stable,but they are unstable in tap water whether they are protected from light or not;oxidants have a great influence on the stability of sanguinarine and chelerythrine;sanguinarine and chelerythrine are stable at room temperature lower than 54℃;sanguinarine is stable in pH 2.5-7.0,while chelerythrine is stable in pH 2.5-8.0.[Conclusions]The good stability of sanguinarine and chelerythrine under specific conditions shows that they have broad development prospects and value.展开更多
Objective:To evaluate the influence of extraction solvents on antioxidant and antimicrobial activities of the pulp and seed of Anisophyllea laurina R.Br.ex Sabine fruits.Methods:The antibacterial activities of pulp an...Objective:To evaluate the influence of extraction solvents on antioxidant and antimicrobial activities of the pulp and seed of Anisophyllea laurina R.Br.ex Sabine fruits.Methods:The antibacterial activities of pulp and seed extracts were tested by using disk diffusion method against eight bacterial strains and three fungal strains.Total phenolic,flavonoid,monomeric anthocyanin and tannin contents,and antioxidant activities were determined by spectrometric methods.Results:The antioxidant analysis of pulp extract revealed the strong radical scavenging capacity and total phenolic content(4 329.66 mg of gallic acid/100 g),while seed extract showed the high antioxidant activity and total tannin content(5 326.78 mg catechin equivalent/100 g).Antibacterial and antifungal activities of methanol and ethanol extracts exhibited potent growth inhibitory activity against Aeromonas hydrophila,Bacillus subtilis,Escherichia coli O157:H7,Pseudomonas aeruginosa,Salmonella Typhimurium and Staphylococcus aureus ATCC 6538 with minimum inhibitory concentration values ranged from 125 to 250 μg/mL.However,seed extract had the strongest potential activity against Aspergillus niger and Candida albicans with minimum inhibitory concentration value of 500 μg/mL compared to pulp extract.Conclusions:Our results therefore demonstrated that ethanol and methanol extractions were more efficient in extracting antioxidants and bioactive compound in pulp and seed.These results support that these plant extracts can be used for the treatment of bacterial infections.展开更多
[ Objective] The paper aimed to study the optimum extraction technology of pigments from petals of Matthiola incana（ L. ） R. Br. of differ- ent colors. [Method] Petal pigments of Aida variety （white violet）, Franc...[ Objective] The paper aimed to study the optimum extraction technology of pigments from petals of Matthiola incana（ L. ） R. Br. of differ- ent colors. [Method] Petal pigments of Aida variety （white violet）, Francesco variety （ red violet） and Arabella variety （ purple violet） were ana- lyzed by colorimetric reaction method and ultraviolet-visible spectrum method, and the optimum extraction technology of flavonoid compounds from petals of them was determined by single factor experiment and orthogonal experiment. [ Result] The optimum extraction conditions of white violet were as follows： with 95% ethanol ＋ 5% HCI as solvent, solid-liquid ratio （ 1 ： 40）, extraction time （3 h）, extraction temperature （65 ℃ ）. The opti- mum extraction conditions of red violet and purple violet were as follows： with acetone as solvent, solid-liquid ratio （1：40）, extraction time （3 h）, extraction temperature （65 ℃）. [ Conclusion] The study provided theoretical foundation for further development and exploitation of pigments from M. incana（ L. ） R. Br.展开更多
A new guanidine derivative,complanatin has been isolated from the seeds of Chinese traditional medicine Astragalus complanatus R.Br..Its structure was established on the basis of spectral data and X-ray crystallograph...A new guanidine derivative,complanatin has been isolated from the seeds of Chinese traditional medicine Astragalus complanatus R.Br..Its structure was established on the basis of spectral data and X-ray crystallographic analysis to be N-[3-carboxypropyl]-N-[3-methyl-2-butenyl]guanidine.展开更多
通过调查,对Livistona chinensis的后选模式、L.fengkaiensis和L.jenkinsiana的主模式、L.saribus的等新模式、L.speciosa的等模式与《Flora of China》中的上述种类作比较,证实《Flora of China》中的L.jenkinsiana是L.spe-ciosaKurz,...通过调查,对Livistona chinensis的后选模式、L.fengkaiensis和L.jenkinsiana的主模式、L.saribus的等新模式、L.speciosa的等模式与《Flora of China》中的上述种类作比较,证实《Flora of China》中的L.jenkinsiana是L.spe-ciosaKurz,后者被中国分类学文献(如《海南植物志》、《中国植物志》、《福建植物志》、《云南植物志》)误定为L.saribus。L.speciosa的果倒卵形、椭圆形或卵形,而L.jenkinsiana的果肾形或近球形。源于《中国植物志》的L.saribus并非原产于中国。在中国,L.speciosa原产于云南、广东、海南和福建,L.chinensis原产于广东和台湾,而L.jenkinsi-ana和L.saribus仅被引种至中国的植物园。展开更多
[Objectives]To study the inhibitory activity of two flavonoid glycosides isolated from Chlorophytum comosum Laxum R.Br on human nasopharyngeal carcinoma(NPC)cell line 5-8F in vitro and its mechanism.[Methods]The flavo...[Objectives]To study the inhibitory activity of two flavonoid glycosides isolated from Chlorophytum comosum Laxum R.Br on human nasopharyngeal carcinoma(NPC)cell line 5-8F in vitro and its mechanism.[Methods]The flavonoid glycosides were isolated and purified from the ethanol alcoholic extract of the roots of Liliaceae plant Chlorophytum comosum by silica gel column chromatography,macroporous resin column chromatography,Sephadex LH-20,and reverse column chromatography(ODS).The inhibitory activity of flavonoid glycosides on human nasopharyngeal carcinoma cells was analyzed by CCK-8 method,and the potential mechanism was preliminarily analyzed by molecular docking.[Results]Two flavonoid glycosides were identified as isovitexin 2″-0-rhamnoside and 7-2″-di-O-β-glucopyranosylisovitexin.Two flavonoid glycosides showed promising inhibitory effect on human nasopharyngeal carcinoma cell line 5-8F,with IC_(50) values of 24.8 and 27.5μmol/L,respectively.Molecular docking results showed that the potential targets of two flavonoid glycosides include CyclinD1,Bcl-2β-Catenin,ILK,TGF-β,in addition,two glycosides showed higher predicted binding affinity towards CyclinD1,which verifies the cytotoxicity of the two compounds on human nasopharyngeal carcinoma cell line 5-8F in vitro.[Conclusions]Two flavonoid glycosides are the active molecules in Chlorophytum comosum that can inhibit the proliferation of human nasopharyngeal carcinoma cells,and have the potential to be used in the research and development of anti nasopharyngeal carcinoma drugs.展开更多
基金Supported by Open Funds for Innovation Platforms of Colleges and Universities in Hunan Province(15K066)National College Students'Innovative Entrepreneuria Training Program(201510553003)Research Study and Innovative Experiment Plan Project for College Students in Hunan Province(2015-499)~~
文摘In order to optimize the microwave-assisted extraction technology of polyphenols from Loropetalum chinense （R. Br.） Oliv., the effects of microwave power, ethanol concentration, solid to liquid ratio and extraction time on polyphenols extraction rate were investigated. On the basis of single-factor test, a four-factor and three-level orthogonal test was designed by response surface method to establish a mathematical model between the response value and various factors. The results showed that the intensity of effect of different influencing factor on polyphenols ex- traction rate ranked as microwave power＇s〉solid to liquid ratio＇s〉extraction time＇s〉 ethanol concentration＇s. The optimum microwave-assisted extraction conditions for polyphenols from L. chinense were as follows： extraction power 254 W, ethanol concentration 60%, extraction time 12.5 rain and solid to liquid ratio 1：17. Under the optimum extraction conditions, the extraction rate of polyphenols from L. chinense was 19.17%.
基金Project(20576142) supposed by the National Natural Science Foundation of China Project(2009DFA31270) supported by the International Cooperation Project of Ministry of Science and Technology of China
文摘A reliable ultrasound-assisted extraction （UAE） method combined with HPLC-UV for quantification of eight active alkaloids in fruits of Macleaya cordata （Willd） R. Br. was developed. The optimization conditions of UAE were obtained by using Box-Behnken design of response surface methodology. Chromatography was carried out using a Kromasil C18 column by gradient elution with 0.1% phosphoric acid aqueous solution for HPLC-UV. All calibration curves showed good linear correlation coefficients （R^2〉0.999 6） and recoveries （from 97.3% to 104.9%） were acceptable. 1,1-diphenyl-2-picrylhydrazyl （DPPH） method was employed to test the antioxidant activity of the extract from the samples. The proposed method was successfully applied to quantifying eight components in nine samples of M.cordata, and significant variations of alkaloid contents and antioxidant aetivity of the samples from different habitats were demonstrated. It presents a powerful proof for the selection of the best sources to extract eight kinds of alkaloids.
基金Supported by Post-doctoral Foundation Project of Heilongjiang Province(LBHZ16094)Special Research Fund Project for Scientific and Technological Innovation Talents in Harbin City(2017RAQXJ115)Doctoral Starting up Project of Harbin University of Commerce(2019DS114).
文摘[Objectives]To explore the stability of sanguinarine and chelerythrine in Macleaya cordata(Willd.)R.Br.[Methods]The solubility and stability of sanguinarine and chelerythrine in seven solvents were measured by HPLC.Besides,the effects of water quality,light source,oxidant,temperature,and pH on stability were investigated.[Results]The solubility and stability of sanguinarine and chelerythrine in methanol and ethanol are good;the stability of sanguinarine and chelerythrine in distilled water and rainwater is not affected by light and is very stable,but they are unstable in tap water whether they are protected from light or not;oxidants have a great influence on the stability of sanguinarine and chelerythrine;sanguinarine and chelerythrine are stable at room temperature lower than 54℃;sanguinarine is stable in pH 2.5-7.0,while chelerythrine is stable in pH 2.5-8.0.[Conclusions]The good stability of sanguinarine and chelerythrine under specific conditions shows that they have broad development prospects and value.
基金Supported by National Natural Science Foundation of China(31271812)the National High Technology Research and Development Program of China(863 Program 2012AA022105C)
文摘Objective:To evaluate the influence of extraction solvents on antioxidant and antimicrobial activities of the pulp and seed of Anisophyllea laurina R.Br.ex Sabine fruits.Methods:The antibacterial activities of pulp and seed extracts were tested by using disk diffusion method against eight bacterial strains and three fungal strains.Total phenolic,flavonoid,monomeric anthocyanin and tannin contents,and antioxidant activities were determined by spectrometric methods.Results:The antioxidant analysis of pulp extract revealed the strong radical scavenging capacity and total phenolic content(4 329.66 mg of gallic acid/100 g),while seed extract showed the high antioxidant activity and total tannin content(5 326.78 mg catechin equivalent/100 g).Antibacterial and antifungal activities of methanol and ethanol extracts exhibited potent growth inhibitory activity against Aeromonas hydrophila,Bacillus subtilis,Escherichia coli O157:H7,Pseudomonas aeruginosa,Salmonella Typhimurium and Staphylococcus aureus ATCC 6538 with minimum inhibitory concentration values ranged from 125 to 250 μg/mL.However,seed extract had the strongest potential activity against Aspergillus niger and Candida albicans with minimum inhibitory concentration value of 500 μg/mL compared to pulp extract.Conclusions:Our results therefore demonstrated that ethanol and methanol extractions were more efficient in extracting antioxidants and bioactive compound in pulp and seed.These results support that these plant extracts can be used for the treatment of bacterial infections.
基金Supported by Natural Science Foundation in Chongqing(CSTC,2006BB1336)Applied Basic Research Program of Chongqing Education Department(KJ060308)
文摘[ Objective] The paper aimed to study the optimum extraction technology of pigments from petals of Matthiola incana（ L. ） R. Br. of differ- ent colors. [Method] Petal pigments of Aida variety （white violet）, Francesco variety （ red violet） and Arabella variety （ purple violet） were ana- lyzed by colorimetric reaction method and ultraviolet-visible spectrum method, and the optimum extraction technology of flavonoid compounds from petals of them was determined by single factor experiment and orthogonal experiment. [ Result] The optimum extraction conditions of white violet were as follows： with 95% ethanol ＋ 5% HCI as solvent, solid-liquid ratio （ 1 ： 40）, extraction time （3 h）, extraction temperature （65 ℃ ）. The opti- mum extraction conditions of red violet and purple violet were as follows： with acetone as solvent, solid-liquid ratio （1：40）, extraction time （3 h）, extraction temperature （65 ℃）. [ Conclusion] The study provided theoretical foundation for further development and exploitation of pigments from M. incana（ L. ） R. Br.
文摘A new guanidine derivative,complanatin has been isolated from the seeds of Chinese traditional medicine Astragalus complanatus R.Br..Its structure was established on the basis of spectral data and X-ray crystallographic analysis to be N-[3-carboxypropyl]-N-[3-methyl-2-butenyl]guanidine.
文摘通过调查,对Livistona chinensis的后选模式、L.fengkaiensis和L.jenkinsiana的主模式、L.saribus的等新模式、L.speciosa的等模式与《Flora of China》中的上述种类作比较,证实《Flora of China》中的L.jenkinsiana是L.spe-ciosaKurz,后者被中国分类学文献(如《海南植物志》、《中国植物志》、《福建植物志》、《云南植物志》)误定为L.saribus。L.speciosa的果倒卵形、椭圆形或卵形,而L.jenkinsiana的果肾形或近球形。源于《中国植物志》的L.saribus并非原产于中国。在中国,L.speciosa原产于云南、广东、海南和福建,L.chinensis原产于广东和台湾,而L.jenkinsi-ana和L.saribus仅被引种至中国的植物园。
基金Supported by Youth Fund Project of Zhaoqing University(QZ202235)Zhaoqing Science and Technology Plan Project(2022040311011).
文摘[Objectives]To study the inhibitory activity of two flavonoid glycosides isolated from Chlorophytum comosum Laxum R.Br on human nasopharyngeal carcinoma(NPC)cell line 5-8F in vitro and its mechanism.[Methods]The flavonoid glycosides were isolated and purified from the ethanol alcoholic extract of the roots of Liliaceae plant Chlorophytum comosum by silica gel column chromatography,macroporous resin column chromatography,Sephadex LH-20,and reverse column chromatography(ODS).The inhibitory activity of flavonoid glycosides on human nasopharyngeal carcinoma cells was analyzed by CCK-8 method,and the potential mechanism was preliminarily analyzed by molecular docking.[Results]Two flavonoid glycosides were identified as isovitexin 2″-0-rhamnoside and 7-2″-di-O-β-glucopyranosylisovitexin.Two flavonoid glycosides showed promising inhibitory effect on human nasopharyngeal carcinoma cell line 5-8F,with IC_(50) values of 24.8 and 27.5μmol/L,respectively.Molecular docking results showed that the potential targets of two flavonoid glycosides include CyclinD1,Bcl-2β-Catenin,ILK,TGF-β,in addition,two glycosides showed higher predicted binding affinity towards CyclinD1,which verifies the cytotoxicity of the two compounds on human nasopharyngeal carcinoma cell line 5-8F in vitro.[Conclusions]Two flavonoid glycosides are the active molecules in Chlorophytum comosum that can inhibit the proliferation of human nasopharyngeal carcinoma cells,and have the potential to be used in the research and development of anti nasopharyngeal carcinoma drugs.
